Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 668,742 C→G E99Q (GAA→CAA)  rsfS ← ribosomal silencing factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi668,7420CG99.0% 329.3 / ‑4.9 96E99Q (GAA→CAA) rsfSribosomal silencing factor
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base G (47/47);  minor base T (1/0);  total (49/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

GGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGG  >  NC_000913_3_KO_pgi/668596‑668888
                                                                                                                                                  |                                                                                                                                                  
ggccggAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACACGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATa                                                                                                                                                 >  2:580479/1‑150 (MQ=255)
 gccggAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATAc                                                                                                                                                >  2:537169/1‑150 (MQ=255)
   cggAATTTCAATCGGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCCCGGCGACAATTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAg                                                                                                                                              <  1:288978/150‑1 (MQ=255)
   cggAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCGCGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAg                                                                                                                                              <  2:1387378/150‑1 (MQ=255)
   cggAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAg                                                                                                                                              >  2:755078/1‑150 (MQ=255)
   cggAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAg                                                                                                                                              <  1:748122/150‑1 (MQ=255)
    ggAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAgg                                                                                                                                             >  1:1268036/1‑150 (MQ=255)
    ggAATTTCAATCAGCTCGAAGGGCATATCTTGCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAgg                                                                                                                                             <  1:927106/150‑1 (MQ=255)
     gAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGc                                                                                                                                            <  1:455711/150‑1 (MQ=255)
     gAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGc                                                                                                                                            <  1:927563/150‑1 (MQ=255)
     gAATTTCAATCAGCGCGAAGGGCATGTCTTTCGGACAACCACGCAGGTACTCGGTAAAACCGGTATGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGGTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGc                                                                                                                                            <  1:1296143/150‑1 (MQ=255)
         ttCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACg                                                                                                                                        <  2:1223495/150‑1 (MQ=255)
           cAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCGGTCCGGCATTTGCGTTCCCACGGCGACAAGTTGCAGCTTCACCCAGCAACTCCAGAGTTGTTCCAGCGCATACAGGCTACGGc                                                                                                                                      >  1:680623/1‑150 (MQ=255)
             aTCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGctc                                                                                                                                    <  2:1268036/150‑1 (MQ=255)
              gcAGCTCGAAGTGCAGTTCTTTCGGAAAACGACGCAGGTAGTCGGTAAAATCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCTCGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGctct                                                                                                                                   <  2:363297/149‑1 (MQ=255)
                aGCTCGAAGGGCATATCCTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGctctct                                                                                                                                 <  1:12054/150‑1 (MQ=255)
                     gAATGGCATATCTGTCGGAAAACGATGCTGGTTGTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCAGTCATTAAATCCAGAGTTTTTCCCATGGATACAGGCGACGGCTCTCTTCCTg                                                                                                                            <  2:540900/150‑1 (MQ=255)
                        gggCATATCTTTCGGAAAACGACGCAGGTTTTCGCGATATCCGTTGCGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATCAACTCCAGAGTTTTTCCAGTTGCTACAGGCGACGGCTCTCTTACTGcct                                                                                                                         <  2:680623/150‑3 (MQ=255)
                        gggCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCAt                                                                                                                         <  1:98167/150‑1 (MQ=255)
                        cagCATATCTTTCGGAAAACGACGCGTGTACGCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCAt                                                                                                                         <  2:738062/148‑1 (MQ=255)
                         ggCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATg                                                                                                                        >  1:746337/1‑150 (MQ=255)
                          gCATATCTGTCGGAAAAGGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGa                                                                                                                       <  2:92013/150‑1 (MQ=255)
                            atatCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACa                                                                                                                     <  1:190499/150‑1 (MQ=255)
                              atCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACa                                                                                                                     >  2:403711/1‑148 (MQ=255)
                              atCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACa                                                                                                                     <  1:403711/148‑1 (MQ=255)
                               tCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATgg                                                                                                                  <  1:270808/150‑1 (MQ=255)
                                cTTTCGGAAAGCGACGCAGGTGCTTGGTAAAGCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGa                                                                                                                 <  2:236317/150‑1 (MQ=255)
                                 tttCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGCCGGCCCTCTTCCCGCACGCAATGGAc                                                                                                                >  2:53738/1‑150 (MQ=255)
                                 tttCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGCAATggtc                                                                                                                >  2:1310848/1‑148 (MQ=255)
                                 tttCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGAc                                                                                                                >  2:37406/1‑150 (MQ=255)
                                  ttCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACa                                                                                                               >  1:950648/1‑150 (MQ=255)
                                    cGGAGAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCAGTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGCCAAt                                                                                                             >  2:1112121/1‑150 (MQ=255)
                                     ggAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACCGCCACTTTCCTGCCTGACATGGACAGTc                                                                                                            >  1:998517/1‑150 (MQ=255)
                                     ggAAAACGACGCAGGTACTCGGTAAAACCCGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGACTTTTCCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATc                                                                                                            <  2:107579/150‑1 (MQ=255)
                                      gAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATca                                                                                                           >  1:422725/1‑150 (MQ=255)
                                      gAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGAGCACGGCTCCCATCCTGCACGACCTggcgaaaaa                                                                                                           >  2:653037/1‑143 (MQ=255)
                                       aaaaCGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATcac                                                                                                          <  1:753185/150‑1 (MQ=255)
                                            gacgCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGTTACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGc                                                                                                     <  1:792179/150‑1 (MQ=255)
                                                   gTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCCACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGAAACAGGCGACGGCTCTCTTCCTGCATGCCATGGACAATGACATCGCACCAATc                                                                                              >  1:1062352/1‑150 (MQ=255)
                                                   gTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCACACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGGCTTCTTCCTGCATTACATGGACAATCGAATCCCCCAAATc                                                                                              >  2:699766/1‑150 (MQ=255)
                                                     aCTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGATTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCa                                                                                            <  2:534441/150‑1 (MQ=255)
                                                      cTCGGTAAAACCGGTTTGTACCCAGTCCGGCGTTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCAc                                                                                           >  1:954675/1‑150 (MQ=255)
                                                      cTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCAc                                                                                           <  2:422725/150‑1 (MQ=255)
                                                       tCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATCGACAATCACATCGCCCAAATCCAcc                                                                                          >  2:192135/1‑149 (MQ=255)
                                                             aaaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATc                                                                                    <  2:950648/150‑1 (MQ=255)
                                                              aaCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCCTGCAATATACTAGCACATCGCCCAAATCCACGACAATcc                                                                                   >  2:679955/1‑150 (MQ=255)
                                                               aCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATAGACAATCACATCGCCCAAATCCACGACAATCCa                                                                                  >  1:1164769/1‑150 (MQ=255)
                                                                 cGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGt                                                                                <  1:1320949/150‑1 (MQ=255)
                                                                   gTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCg                                                                              >  2:964529/1‑150 (MQ=255)
                                                                   gTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCg                                                                              >  1:1145792/1‑150 (MQ=255)
                                                                   gTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGCCg                                                                              >  2:576292/1‑150 (MQ=255)
                                                                       gTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGgcgc                                                                          >  1:951865/1‑150 (MQ=255)
                                                                        tACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCt                                                                         >  2:1305838/1‑150 (MQ=255)
                                                                         aCCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGa                                                                                                                 >  2:867858/1‑109 (MQ=255)
                                                                         aCCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGa                                                                                                                 <  1:867858/109‑1 (MQ=255)
                                                                          cccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAAt                                                                                                             >  2:1300413/1‑112 (MQ=255)
                                                                          cccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAAt                                                                                                             <  1:1300413/112‑1 (MQ=255)
                                                                           ccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCCCGTTGACTACAATCCAGTCGGCGCTGtt                                                                      >  2:1101210/1‑150 (MQ=255)
                                                                           ccAGTCCGGCATTTTCGTTCCCACGGCGACAAGATGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGtt                                                                      <  1:37406/150‑1 (MQ=255)
                                                                               tCCGGCATTTTCGTTCCCACGGCGAGAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTTTTTTTTTTTTTAACAACGGCTCTCCTCCTGCATTATATGGACAATTACATCGCCCAAATCGCCGCCAATCCTGGCCGCGCTCTTTTCa                                                                  >  2:903649/1‑150 (MQ=255)
                                                                               tCCGGCATTTTCGTTCCCACCGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACCATCCAGTCGGCGCTGTTTTCa                                                                  <  1:1036724/150‑1 (MQ=255)
                                                                                ccGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCAc                                                                 <  1:1246072/150‑1 (MQ=255)
                                                                                      ttttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGCCGCTGTTTTCCCCTTCtc                                                           >  2:259896/1‑149 (MQ=255)
                                                                                        ttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACg                                                         >  2:1058143/1‑150 (MQ=255)
                                                                                        ttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGCAGGCGCGGTTTTCACCTTCTAcc                                                         >  1:763741/1‑149 (MQ=255)
                                                                                           gTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACATGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCCCGACAATCAAGTCCGCGCTGTTTTCACCTTCTGCGCCg                                                      >  1:1231177/1‑150 (MQ=255)
                                                                                               ccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCg                                                  >  2:135744/1‑150 (MQ=255)
                                                                                                 aCGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGt                                                >  1:533055/1‑150 (MQ=255)
                                                                                                   ggCGATAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTaa                                              <  2:181945/150‑1 (MQ=255)
                                                                                                   ggCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTaa                                              <  1:1058143/150‑1 (MQ=255)
                                                                                                        cAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCGGTTTTCACCTTCTCCGCCGACCGGTAACGGGc                                         >  2:331215/1‑150 (MQ=255)
                                                                                                              gCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTg                                   >  1:1247925/1‑150 (MQ=255)
                                                                                                                           ttAACTCCAGAGTTTTTCCCGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTg                      >  1:244555/1‑150 (MQ=255)
                                                                                                                            taccTCCAGAGTTTTTCCAGTTGATACCGGGGACGGCTCTTTTCCTGCATGACATGGACAATCGCATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGc                     <  2:481392/147‑1 (MQ=255)
                                                                                                                            tAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCCGGCGGTAACAGGCCCGCTGCGCGAGACTCCTGc                     >  1:35026/1‑150 (MQ=255)
                                                                                                                                tCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACACTCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACaa                 >  1:263474/1‑150 (MQ=255)
                                                                                                                                tCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAt                                                                                     >  1:627642/1‑82 (MQ=255)
                                                                                                                                tCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAAt                                                                                     <  2:627642/82‑1 (MQ=255)
                                                                                                                                tCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACaa                 >  2:338024/1‑150 (MQ=255)
                                                                                                                                 ccAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAAc                <  1:754253/150‑1 (MQ=255)
                                                                                                                                  cAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACg               >  2:1362017/1‑150 (MQ=255)
                                                                                                                                   agagTTTTTCCAGTTGATACAGGCGACGGTTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGt              >  2:692862/1‑150 (MQ=255)
                                                                                                                                   agagTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGt              <  1:259896/150‑1 (MQ=255)
                                                                                                                                   agagTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGt              <  1:477669/150‑1 (MQ=255)
                                                                                                                                    gagTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTg             >  2:1451525/1‑150 (MQ=255)
                                                                                                                                    gagTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTg             <  2:954675/150‑1 (MQ=255)
                                                                                                                                    gagTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTg             <  1:692862/150‑1 (MQ=255)
                                                                                                                                      gttgttCCAGTTGATACAGGCGACGGCGCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGt           <  2:1062352/150‑1 (MQ=255)
                                                                                                                                       tttttCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCTCGACAATTCAGTCAGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTc          >  1:468419/1‑150 (MQ=255)
                                                                                                                                         tttCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAg        <  2:1426710/150‑1 (MQ=255)
                                                                                                                                          ttCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGc       >  1:1137805/1‑150 (MQ=255)
                                                                                                                                            ccAGTTGATACAGGCGACGGCGCTCTTCCGGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCGGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCaa     <  2:244555/150‑1 (MQ=255)
                                                                                                                                              aGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATg   <  1:1101210/150‑1 (MQ=255)
                                                                                                                                              aGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGCCACCAATg   >  2:352885/1‑150 (MQ=255)
                                                                                                                                              aGTTGATACAGGCGACGCCTCTCTTCCTGCATGACATGGACAACCTCATCGCCCAACTCCACGACAATCCAGTCGGCGCGGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATg   <  1:53738/150‑1 (MQ=255)
                                                                                                                                               gTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACAGCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATgg  <  1:218392/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATGG  >  NC_000913_3_KO_pgi/668596‑668888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: