Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 2,513,351 C→A L104M (CTG→ATG)  nupC → nucleoside (except guanosine) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi2,513,3510CA98.7% 261.8 / NA 76L104M (CTG→ATG) nupCnucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (41/34);  minor base G (1/0);  total (42/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAACT  >  NC_000913_3_KO_pgi/2513205‑2513492
                                                                                                                                                   |                                                                                                                                             
gCTTCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGa                                                                                                                                            >  2:1203697/1‑150 (MQ=255)
    cGTGAGAGGCTTCTCTAAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCgg                                                                                                                                        >  2:1265936/1‑150 (MQ=255)
     gTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGt                                                                                                                                       <  2:201322/150‑1 (MQ=255)
         aaGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATtc                                                                                                                                   <  2:620264/150‑1 (MQ=255)
          aGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTCCATCCAGAAAGGGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATtcc                                                                                                                                  >  2:1323797/1‑149 (MQ=255)
           ggCTTCTCCGAAATGTTCGAAGAACTGCTCGGAGTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATtctc                                                                                                                                 <  1:675906/150‑1 (MQ=255)
           ggCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATtctc                                                                                                                                 <  2:139442/150‑1 (MQ=255)
                ctcCGAAATGTTCGAAAAACTGCTCGGATTTGACAATGAATGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGca                                                                                                                            <  2:1390254/150‑1 (MQ=255)
                  ccGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGcaca                                                                                                                          >  1:365527/1‑150 (MQ=255)
                  ccGAAATGTTCGAAAAACTGCTCGGAGTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAGGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGcaca                                                                                                                          >  2:769328/1‑150 (MQ=255)
                     aaaTGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTc                                                                                                                       >  1:943400/1‑150 (MQ=255)
                              aaaaaCTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCTATGATCGGTATTCTCCAGCACATTCGCGTGTTGc                                                                                                              <  2:1152134/150‑1 (MQ=255)
                                    tGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGa                                                                                                        <  1:215072/150‑1 (MQ=255)
                                     gCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGat                                                                                                       <  2:622411/150‑1 (MQ=255)
                                      cTCGGATTTGCCAACGGAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAGTGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGatc                                                                                                      >  1:1270422/1‑150 (MQ=255)
                                          gATTTGCCGACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATcc                                                                                                  <  1:1323797/150‑1 (MQ=255)
                                          gATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATcc                                                                                                  <  1:1265936/150‑1 (MQ=255)
                                            tttGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCcgc                                                                                                <  1:731948/150‑1 (MQ=255)
                                             ttGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCcgcg                                                                                               <  2:943400/150‑1 (MQ=255)
                                              tGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGAGCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCgcgc                                                                                              <  2:1408238/150‑1 (MQ=255)
                                                ccAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCaa                                                                                            >  1:1009224/1‑150 (MQ=255)
                                                ccAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCaa                                                                                            >  2:979845/1‑150 (MQ=255)
                                                 cAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGAGTGTTGCCGGTGATCATCCGCGCAAt                                                                                           >  2:1131149/1‑150 (MQ=255)
                                                  aaCGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAAtt                                                                                          >  2:1291362/1‑150 (MQ=255)
                                                           aCTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCtc                                                                                 >  2:44855/1‑149 (MQ=255)
                                                                 ttCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTcc                                                                           >  1:164505/1‑150 (MQ=255)
                                                                 ttCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCAGAGCAACTAGTTTCTTGCTCTcc                                                                           >  2:602448/1‑150 (MQ=255)
                                                                 ttCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGCGATCATCCGCGCAATTGGTTTCCTGCTCTcc                                                                           >  2:1349184/1‑150 (MQ=255)
                                                                   cGTCTTTGGTAGCATGAATGATCAAGCAAAGCATGGCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCaa                                                                         >  1:357670/1‑150 (MQ=255)
                                                                    gTCTTTGTTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCACTCGGTTTCCTGCTCTTCaaa                                                                        <  2:871850/150‑1 (MQ=255)
                                                                    gTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATAGTTTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCaaa                                                                        >  1:1012196/1‑150 (MQ=255)
                                                                     tCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAg                                                                       >  2:1326937/1‑150 (MQ=255)
                                                                       tttGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCgt                                                                                                                   <  2:866539/104‑1 (MQ=255)
                                                                       tttGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCgt                                                                                                                   >  1:866539/1‑104 (MQ=255)
                                                                       tttGGGAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTc                                                                     >  2:989276/1‑150 (MQ=255)
                                                                          ggTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAAc                                                                  <  1:1291362/150‑1 (MQ=255)
                                                                               cATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCAt                                                             >  1:762824/1‑150 (MQ=255)
                                                                                atgaatgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATg                                                            <  1:1203697/150‑1 (MQ=255)
                                                                                atgaatgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATg                                                            <  2:1410351/150‑1 (MQ=255)
                                                                                   aatgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGc                                                         <  2:1270422/150‑1 (MQ=255)
                                                                                     tgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATg                                                            <  2:1395098/145‑1 (MQ=255)
                                                                                     tgaTCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATg                                                            >  1:1395098/1‑145 (MQ=255)
                                                                                        tCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGGTCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACt                                                    >  1:1045387/1‑150 (MQ=255)
                                                                                          aaGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTgg                                                  >  2:359574/1‑150 (MQ=255)
                                                                                            gatcTGTCATTCTTCTTCCTGAAAGTGCTGTGCCAAATCGGCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGaa                                                <  2:108482/147‑1 (MQ=255)
                                                                                                tGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTCTTTAACGGGACGTTCGCGTGTTGCCGGTGATCATACGCGCAATTTGGTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCt                                            >  1:1429502/1‑150 (MQ=255)
                                                                                                  gCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCttt                                          <  1:802459/150‑1 (MQ=255)
                                                                                                  gCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGAGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGattagatc                                          >  1:108482/1‑143 (MQ=255)
                                                                                                  gCATTCTTCCTCCTTAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATAATTCGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCttt                                          <  1:341787/150‑1 (MQ=255)
                                                                                                    aTTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTaa                                        <  1:1275741/150‑1 (MQ=255)
                                                                                                         ttcttcCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTTTCCAAAATCAACGGCATGGGCAAACTGGAATCCTTTAACGcc                                    >  2:1174133/1‑150 (MQ=255)
                                                                                                         ttcttcCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCACACTGGAATCCTTTAACGcc                                    >  2:837492/1‑150 (MQ=255)
                                                                                                         ttcttcCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGcc                                    >  1:1336567/1‑150 (MQ=255)
                                                                                                              ccTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAg                               <  2:357670/150‑1 (MQ=255)
                                                                                                               cTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAAc                                                                  <  1:306020/114‑1 (MQ=255)
                                                                                                               cTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAAc                                                                  >  2:306020/1‑114 (MQ=255)
                                                                                                               cTGAAAGTGCGGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCCACAAATTCAACGGCCTGGGCCAACTGGAACCGCTTACCGCCGTCAGc                              >  2:401005/1‑150 (MQ=255)
                                                                                                                tGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAACCTGGAATCCTTTAACCCCGTCAGCt                             >  1:84635/1‑150 (MQ=255)
                                                                                                                tGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGCTGATCATCCGCGCTATTGGGTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGcc                             >  2:1252960/1‑149 (MQ=255)
                                                                                                                    aGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCAt                                                             >  2:947061/1‑114 (MQ=255)
                                                                                                                    aGTGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCAt                                                             <  1:947061/114‑1 (MQ=255)
                                                                                                                      tGCTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGa                       >  1:530550/1‑150 (MQ=255)
                                                                                                                        cTGTGCCCAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGAtt                     >  1:393316/1‑150 (MQ=255)
                                                                                                                              ccAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCCCATTCGCGTGTTGCCGGTGAGCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCCTGTGCAAGCTGGAATCCTTTAACGCCGTCAGCTCCCTGATGCTGGGt               >  1:946892/1‑150 (MQ=255)
                                                                                                                              ccAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGt               <  2:1336567/150‑1 (MQ=255)
                                                                                                                              ccAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGt               <  1:752251/150‑1 (MQ=255)
                                                                                                                              ccAATCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGCGt               <  2:762824/150‑1 (MQ=255)
                                                                                                                                aaTCGTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCa             >  2:595957/1‑150 (MQ=255)
                                                                                                                                    gTCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCt         >  1:839079/1‑150 (MQ=255)
                                                                                                                                     tCTTTATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGATTTCCTGCTCTCCAATGTCAACGACATGGGCAACCTGGAATCCTCTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTg        >  1:1293346/1‑150 (MQ=255)
                                                                                                                                       tttATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGaa      >  1:1076023/1‑150 (MQ=255)
                                                                                                                                       tttATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGaa      <  2:1009224/150‑1 (MQ=255)
                                                                                                                                         tATCTCTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGaaaa    <  2:1209927/150‑1 (MQ=255)
                                                                                                                                           tctcTGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAACt  <  1:979845/150‑1 (MQ=255)
                                                                                                                                               tGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGa                                                                                                        >  2:761070/1‑44 (MQ=255)
                                                                                                                                               tGCAATGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGa                                                                                                        <  1:761070/44‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
GCTTCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGTAGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTATTCTCCAGCACATTCGCGTGTTGCCGGTGATCATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGCATGGGCAAACTGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCTGAAAACT  >  NC_000913_3_KO_pgi/2513205‑2513492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: