Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_KO_pgi	3,440,086	C→A	G315V (GGC→GTC)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_KO_pgi	3,440,086	0	C	A	100.0%	277.7 / NA	84	G315V (GGC→GTC)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (42/42);  total (42/42)

TGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAG  >  NC_000913_3_KO_pgi/3439947‑3440226                                                                                                                                            |                                                                                                                                             tGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAg                                                                                                                                    >  1:1382800/1‑150 (MQ=255) tGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAg                                                                                                                                    >  2:39208/1‑150 (MQ=255)             gtgTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGGATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATGCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGc                                                                                                                        <  2:333494/149‑1 (MQ=255)                  gCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCGTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCa                                                                                                                   <  2:934283/150‑1 (MQ=255)                       tCAGTTGACGACCACTCGTACGATGGCGCATGACCTTATCCTGCTCAGTAAAACCTTAGCCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCt                                                                                                              <  1:1367432/150‑1 (MQ=255)                        cAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGGTGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCtt                                                                                                             <  2:970401/150‑1 (MQ=255)                         aGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCttt                                                                                                            >  1:872623/1‑150 (MQ=255)                          gTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCAGGGGAAGCCAGCACGTCTTTa                                                                                                           >  1:1344586/1‑150 (MQ=255)                          gTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCGTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTa                                                                                                           <  1:800121/150‑1 (MQ=255)                           ttGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCCCGTCTTTaa                                                                                                          >  1:492001/1‑150 (MQ=255)                                gacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAAtctcc                                                                                                     >  1:1327947/1‑149 (MQ=255)                                gacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCa                                                                                                     <  1:861116/150‑1 (MQ=255)                                gacCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCa                                                                                                     >  1:293315/1‑150 (MQ=255)                                 acCACTCTTACGATGGCGCATGACCTTAGCCTTCGCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTTCCGGTGGCCAGTTTGCCAGGCGCATGACCAGAGACAGTCCACGGGGAGCCAGCACGTCTTTAATCTCAg                                                                                                    >  1:313283/1‑150 (MQ=255)                                      tctTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAaga                                                                                               >  2:96170/1‑150 (MQ=255)                                      tctTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAaga                                                                                               >  1:176656/1‑150 (MQ=255)                                        ttACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCCGGCGCATGACCAGAGACAGTCCACGGGACGCCAGCACGTCTTTAATCCCAgaacgaac                                                                                             >  2:41172/1‑143 (MQ=255)                                          aCGATGGCGCATGACCTTATCCTTCTCAGTAAAACTTTTACCTTTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAtt                                                                                           <  1:1355664/150‑1 (MQ=255)                                              tGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAg                                                                                                                                    <  2:41879/105‑1 (MQ=255)                                              tGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAg                                                                                                                                    >  1:41879/1‑105 (MQ=255)                                               ggCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGAttttttc                                                                                      <  2:293315/150‑2 (MQ=255)                                               ggCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTa                                                                                      >  2:1115914/1‑150 (MQ=255)                                                   cATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCt                                                                                                              >  1:328003/1‑122 (MQ=255)                                                   cATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCt                                                                                                              <  2:328003/122‑1 (MQ=255)                                                   cATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCaa                                                                                  <  1:39208/150‑1 (MQ=255)                                                    aTGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAg                                                                                 <  2:813852/150‑1 (MQ=255)                                                      caCCTCACCCTTCACAGTAAACCCTTAACCTGTGATCCCTGTACTCCTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGATACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGCAAGAGATTTTTTACCAAGGt                                                                               <  1:268/149‑1 (MQ=255)                                                       aCCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTACGAGAGTTTTTTCCAAGGtt                                                                              >  1:815164/1‑150 (MQ=255)                                                        ccTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTa                                                                             <  2:786433/150‑1 (MQ=255)                                                          ttATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAgg                                                                           <  1:453217/150‑1 (MQ=255)                                                          ttATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAgg                                                                           <  2:627594/150‑1 (MQ=255)                                                          ttATCCTGTTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAgg                                                                           <  2:257533/150‑1 (MQ=255)                                                           tATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACgg                                                                                                                             >  2:152348/1‑99 (MQ=255)                                                           tATCCTTCTCAGTAAAACCTTAACCTGTGAGCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACgg                                                                                                                             <  1:152348/99‑1 (MQ=255)                                                            aTCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGGGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGAAAGAGATTTTTTACCAAGGTTCGGCg                                                                         >  2:432833/1‑150 (MQ=255)                                                              ccTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACATTCCACGGGAAGCCAGCACGTCTTTCATCTCAGTAAGAGATTTTTTACCCAGGTTAGGCGtt                                                                       >  1:1031141/1‑150 (MQ=255)                                                              ccTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGtt                                                                       <  1:815916/150‑1 (MQ=255)                                                              ccTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGtt                                                                       <  2:176656/150‑1 (MQ=255)                                                               cTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCtt                                                                                                             >  2:374531/1‑111 (MQ=255)                                                               cTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCtt                                                                                                             <  1:374531/111‑1 (MQ=255)                                                                ttCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGtttt                                                                     >  1:162662/1‑150 (MQ=255)                                                                ttCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGtttt                                                                     >  2:57078/1‑150 (MQ=255)                                                                  ctcATTAAAACCTTAACCTCTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCCGTTTTCCAGGCGCATGACCAGCGAAAGTCCACATGAAGCCAGCACGTCTCGAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTaa                                                                   <  1:282525/150‑1 (MQ=255)                                                                  ctcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTaa                                                                   <  2:872623/150‑1 (MQ=255)                                                                   tcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                  <  2:24863/150‑1 (MQ=255)                                                                   ccAGTAAAACCTTACCCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                  <  2:1285161/149‑1 (MQ=255)                                                                     aGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAAATTTTTTACCAAGGTTCGCAGGTTTAAGGa                                                                >  2:334487/1‑150 (MQ=255)                                                                      gTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAg                                                               <  1:96170/150‑1 (MQ=255)                                                                                aaCCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                     <  2:718827/150‑1 (MQ=255)                                                                                 aCCTGTGATCCGGTTACTCGGCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCgg                                                    <  2:776985/150‑1 (MQ=255)                                                                                   cTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTaa                                                                                                 >  2:473265/1‑103 (MQ=255)                                                                                   cTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTaa                                                                                                 <  1:473265/103‑1 (MQ=255)                                                                                    tgtgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCCCAACCTCGGTAc                                                 >  2:914034/1‑150 (MQ=255)                                                                                    tgtgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGGGTTCCAGGCGCATGACCAGCGCAAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTAc                                                 >  1:1141088/1‑150 (MQ=255)                                                                                        aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAAc                                                         >  2:815384/1‑138 (MQ=255)                                                                                        aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAAc                                                         <  1:815384/138‑1 (MQ=255)                                                                                        aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTg                                             >  2:434640/1‑150 (MQ=255)                                                                                        aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTg                                             <  1:234327/150‑1 (MQ=255)                                                                                         tCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGt                                            >  1:1402862/1‑150 (MQ=255)                                                                                             gTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCa                                        >  1:440432/1‑150 (MQ=255)                                                                                              ttACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACa                                                                                                                                     >  2:678842/1‑56 (MQ=255)                                                                                              ttACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACa                                                                                                                                     <  1:678842/56‑1 (MQ=255)                                                                                              ttACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAg                                       >  2:470431/1‑150 (MQ=255)                                                                                                   cGTCAGCGATGCTTGCCGGTGGCTAGTTTTCCAGGAGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAc                                  <  1:1279502/150‑1 (MQ=255)                                                                                                    gTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAcc                                 >  1:316158/1‑150 (MQ=255)                                                                                                    gTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCGCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAcc                                 <  1:1115914/150‑1 (MQ=255)                                                                                                     tCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCg                                >  2:1354195/1‑150 (MQ=255)                                                                                                       aGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGCCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCACCCTCGGTACGCTGTACCAGATAACCGat                              >  2:714131/1‑150 (MQ=255)                                                                                                           aTGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGATAGTCCACGGGAAGTCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAg                          <  2:1214149/150‑1 (MQ=255)                                                                                                            ttcTTGCCGGTGGCCAGTGTTCCAGGCGGATGACTAGAGACAGTCCACTGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGt                         <  2:1063771/148‑1 (MQ=255)                                                                                                               ttGCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGa                      <  2:162662/150‑1 (MQ=255)                                                                                                                 gCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGAt                                     >  2:1326854/1‑133 (MQ=255)                                                                                                                 gCCGGTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGAt                                     <  1:1326854/133‑1 (MQ=255)                                                                                                                     gTGGCCAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCGGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCtt                >  1:1268145/1‑150 (MQ=255)                                                                                                                      tGGCGAGTTTTCCAGGCGAATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTc               <  2:26356/150‑1 (MQ=255)                                                                                                                          cAGTTTTCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCt           >  1:137860/1‑150 (MQ=255)                                                                                                                           aGTTTTCCAGGCGCATGACCAGAGATAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCtt          >  2:541148/1‑150 (MQ=255)                                                                                                                              tttCCAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTaa       <  1:1303212/150‑1 (MQ=255)                                                                                                                                  cAGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACAGATATAGTGGATAGCTTCTGCTTTGAGGCa   >  2:236941/1‑150 (MQ=255)                                                                                                                                   aGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg             <  2:178351/139‑1 (MQ=255)                                                                                                                                   aGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg             >  1:178351/1‑139 (MQ=255)                                                                                                                                   aGGCGCATGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAg  >  2:768147/1‑150 (MQ=255)                                                                                                                                    ggCGCGTGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAg                                                                                 <  1:1263838/70‑1 (MQ=255)                                                                                                                                    ggCGCCTGACCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAg                                                                                 >  2:1263838/1‑70 (MQ=255)                                                                                                                                            |                                                                                                                                             TGGCGATGGCTGCTGTTGCGGTTCAGTTGACGACCACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAG  >  NC_000913_3_KO_pgi/3439947‑3440226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑