New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 4404097	45 (1.240)	5 (0.140)	5/276	4.3	10.1%	coding (977/1299 nt)	purA	adenylosuccinate synthetase
	?	NC_000913_3_KO_pgi	= 4404109	45 (1.260)					coding (989/1299 nt)	purA	adenylosuccinate synthetase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/4403965‑4404097 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtcAGGCAGAAGCCAGACAGGGAGTTCAGCTGTACCGCACGACGAACGGCAACGGTGTCCAGCCAGCCGGTACGACGACGACGCCCCGTAGTTGCGCCGAATTCGTTACCCTGCTTGCAGAGGAACTCGCCAGTTTCATCAAACAGTTCG  <  NC_000913_3_KO_pgi/4404109‑4403963                                                                                                                                                                                                                                                                                          aagTGTTGGATGAAACTGGCGGCTTCCGCTGCAAGCAGGGTAACGAATTAGGGGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCAGGCAGAAGCCAGACA                                                                                                                                     <  2:48076/146‑1           TGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCAGGCAGAAGCCAGAC                                                                                                                                      >  1:228290/1‑138           TGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCAGGCAGAAGCCAGAC                                                                                                                                      <  2:228290/138‑1                                                                                        TGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTCAGGCAGAAGCCAGACAGGGAGTTCAGCTGTAC                                                                                                                     <  2:319648/78‑1                                                                                        TGGACACCGTTGCCGTTCGTCGTGCGGTAAAGCTGAACTCCCTGTCAGGCAGAAGCCAGACAGGGAGTTCAGCTGTAC                                                                                                                     >  1:319648/1‑78                                                                                                                                    TCAGGCAGAAGCCAGACAGGGAGTTCAGCTGTACCGCACGACGAACGGCAACGGTGTCCAGCCAGCCGGTACGACGACGACGCCCCGTAGTTGCGCCGAATTCGTTACCCTGCTTGCAGAGGAACTCGCCCGTTTCGTCAAACAGTTCG  >  1:247667/1‑149                                                                                                                                                                                                                                                                                          AACTGTTTGATGAAACTGGCGAGTTCCTCTGCAAGCAGGGTAACGAATTCGGCGCAACTACGGGGCGTCGTCGTCGTACCGGCTGGCTGGACACCGTTGCCGTTCGTCGTGCGGTACAGCTGAACTCCCTGTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/4403965‑4404097 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtcAGGCAGAAGCCAGACAGGGAGTTCAGCTGTACCGCACGACGAACGGCAACGGTGTCCAGCCAGCCGGTACGACGACGACGCCCCGTAGTTGCGCCGAATTCGTTACCCTGCTTGCAGAGGAACTCGCCAGTTTCATCAAACAGTTCG  <  NC_000913_3_KO_pgi/4404109‑4403963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.