New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 237489336 (0.990)5 (0.140) 5/280 4.4 12.7% coding (111/1356 nt) menE O‑succinylbenzoate‑CoA ligase
?NC_000913_3_KO_pgi = 2374914 33 (0.910)coding (90/1356 nt) menE O‑succinylbenzoate‑CoA ligase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGATCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCA  >  NC_000913_3_KO_pgi/2374771‑2375056
                                                                                                                                               |                                                                                                                                              
cgcgCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCagtt                                                                                                                                           <  2:65789/149‑1 (MQ=255)
 gcgcCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCagttg                                                                                                                                          >  1:505537/1‑149 (MQ=255)
  cgcCCCGCATGGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCagttga                                                                                                                                         <  2:178841/149‑1 (MQ=255)
       cGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACCCCGCTGCCCTCAACCACCCCCTGCCCCGCAAATCCGGAGGCTAATTCATCGACGCGCGCACCAAGCTCCCGCCCGTTGAGCTGc                                                                                                                                    >  1:502312/1‑149 (MQ=255)
             gCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCa                                                                                                                              >  1:339212/1‑149 (MQ=255)
                   aCGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGa                                                                                                                        <  2:595770/149‑1 (MQ=255)
                   aCGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGa                                                                                                                        <  2:353424/149‑1 (MQ=255)
                   aCGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGa                                                                                                                        >  1:456997/1‑149 (MQ=255)
                          ccagGCGAGCAGCGTTTGCGGCTTATCCCTCGCCCGCAACACCCCACCGCGGCCCTCTACCCCCCCCTGAACGGCCGCCCGGGCCGCTGCGTCATCGACCCGGGCCCGAAGCGCGCGCCACATGGGTAGCCCGTCACTCAGACGTAAgg                                                                                                                 >  2:47818/1‑149 (MQ=255)
                              gCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGAt                                                                                                             <  2:438080/149‑1 (MQ=255)
                                 agcagcGTTTGCGGCGTATTCCACGCCCGAAATATCACGCGGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGt                                                                                                          <  2:103403/149‑1 (MQ=255)
                                                 tATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTgc                                                                                          <  1:379701/149‑1 (MQ=255)
                                                   ttCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTgccg                                                                                        >  2:194167/1‑149 (MQ=255)
                                                   ttCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACAACGAATGCTGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCCCACTTgccg                                                                                        >  2:428637/1‑149 (MQ=255)
                                                      cACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCa                                                                                     <  1:75785/149‑1 (MQ=255)
                                                      cACGCCCGCAACATCACCCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCa                                                                                     <  1:256827/149‑1 (MQ=255)
                                                       aCGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAg                                                                                    <  1:194167/149‑1 (MQ=255)
                                                            cGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACg                                                                               <  1:157233/149‑1 (MQ=255)
                                                                              cccTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCaga                                                                <  1:95760/146‑1 (MQ=255)
                                                                              cccTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCaga                                                                >  2:95760/1‑146 (MQ=255)
                                                                                cTCCACCACCCCCGGCACCGCAAATCCGGAGGATAATTCAACGACGGGAGCACAAAGCGCGCGCCATTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTGCTCCCCGCACTTGCCGCCAATGACGCCACGGCCAGTCAGagaaga                                                           <  2:534481/149‑1 (MQ=255)
                                                                                cTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGagaaga                                                           <  1:380267/149‑1 (MQ=255)
                                                                                     ccaccCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGGCAGAGAAGATCATa                                                      >  2:288368/1‑149 (MQ=255)
                                                                                          cccTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGCTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGATCATaacaac                                                 <  2:548600/149‑1 (MQ=255)
                                                                                           ccTGCACCGCAAATCCGGAGGCTAATTCATCGCCGCGCGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCa                                                                      >  2:302881/1‑127 (MQ=255)
                                                                                           ccTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCa                                                                      <  1:302881/127‑1 (MQ=255)
                                                                                                  cGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGATCATAACAACCGCTcccg                                         >  1:69390/1‑147 (MQ=255)
                                                                                                   gCAAATCCGGAGTGTAATTTATCGACGCGATCACAAAGCTCGCGCCCGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGATCATAACAACCGCTCCAGt                                        <  1:373803/149‑1 (MQ=255)
                                                                                                           ggAGGCTAATTCACCGACGCCAGCACAAAGCTCCCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGGCGCTACTGACGCCACGGCCAGTCAGAGAAGATCATAACAACCGCTCCAGTGCATCAAc                                <  1:601325/149‑1 (MQ=255)
                                                                                                                cTAATTCAGCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGATCATAACAACCGCTCCAGTGCATCAACTTCCa                           <  2:287116/149‑1 (MQ=255)
                                                                                                                       aTCGACGCGAGCACCAAGCTAGCGCCAGTTGAGTTGCTCGTCATTAAGCCGTAAGGCGATGGTTTATCCCCGCACTTGCCGCCAGTGACGCCACGGCCCGTCAGAGACGATCATAACACACGCTCACATGCAACAACTTCCACGGCAgg                    >  2:339316/1‑149 (MQ=255)
                                                                                                                                  cacaAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGATCATAACAACCGCTCCAGTGCATCAACTTCCCCGACAGGCAGCGtactac         >  2:414972/1‑149 (MQ=255)
                                                                                                                                         cTCGCTCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGATCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCa  <  1:264580/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
CGCGCCCCGCATTGCAGTAACGCCAGCCAGGCGAGCAGCGTTTGCGGCGTATTCCACGCCCGCAACATCACGCCGCTGCCCTCAACCACCCCCTGCACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGATCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCA  >  NC_000913_3_KO_pgi/2374771‑2375056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.