| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_000913_3_KO_pgi | 4,233,757 | +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC | intergenic (‑524/‑583) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913_3_KO_pgi | = 4233757 | 0 (0.000) | 26 (1.060) +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC |
22/190 | 0.2 | 100% | intergenic (‑524/‑583) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| ? | NC_000913_3_KO_pgi | 4233758 = | 0 (0.000) | intergenic (‑525/‑582) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein | |||||
GGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCG > NC_000913_3_KO_pgi/4233611‑4233847 | actcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcgggcactacgg > 2:489105‑M2/148‑149 (MQ=255)actcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcagacactacgg > 1:187161‑M2/148‑149 (MQ=255) aacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaagatcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTa > 1:183599‑M2/144‑149 (MQ=255) attacgctaacggcactaaaaccatcacatttttttttttttttcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGc > 2:438856‑M2/141‑149 (MQ=255) taacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactccGGTGTAGGCTGGAgct > 2:346841‑M2/134‑149 (MQ=255) taaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTcgagg > 2:30809‑M2/125‑147 (MQ=255) aaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGATGGAGCTGCTTCGAAGTTc < 2:538231‑M2/28‑1 (MQ=255) catcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGCGTAGGCTGGAGCTGCTTCGAAGTTCCta > 2:57572‑M2/119‑149 (MQ=255) acatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACt < 2:621329‑M2/35‑1 (MQ=255) catttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACtt > 1:452643‑M2/114‑149 (MQ=255) gtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGCAGTGCCTAAGCTTTCTAGAGaa > 1:117055‑M2/105‑149 (MQ=255) ctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTc < 1:356882‑M2/55‑1 (MQ=255) tacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatccacccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCg > 1:92516‑M2/94‑149 (MQ=255) caatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGaa < 2:218358‑M2/58‑1 (MQ=255) ttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCa > 2:360156‑M2/87‑149 (MQ=255) aaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTc > 2:195695‑M2/83‑149 (MQ=255) aaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTc < 1:412414‑M2/67‑1 (MQ=255) agtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGa < 1:439106‑M2/69‑1 (MQ=255) gtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTc > 1:311698‑M2/80‑107 (MQ=255) gtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTc < 2:311698‑M2/28‑1 (MQ=255) tcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctcgcaggcactacGGAGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACg > 2:114‑M2/79‑149 (MQ=255) tctcaaaatcagaagagtattgcaaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATcccc < 1:392474‑M2/78‑1 (MQ=255) ctcaagatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCg < 1:103674‑M2/79‑1 (MQ=255) ctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCg < 2:187161‑M2/79‑1 (MQ=255) gaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCcccggaatgctcga > 1:529220‑M2/61‑143 (MQ=255) aagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATccccggaatgctcgac < 1:195695‑M2/90‑8 (MQ=255) tcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATa < 1:5687‑M2/47‑1 (MQ=255) tcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATa > 2:5687‑M2/37‑83 (MQ=255) | GGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCG > NC_000913_3_KO_pgi/4233611‑4233847 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |