Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913_3_KO_pgi	4,233,840	+GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA	intergenic (‑607/‑500)	lysC ← / → yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 4233840	0 (0.000)	21 (0.860) +GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA	18/190	0.4	100%	intergenic (‑607/‑500)	lysC/yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
	?	NC_000913_3_KO_pgi	4233841 =	NA (NA)					intergenic (‑608/‑499)	lysC/yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

AATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGAT  >  NC_000913_3_KO_pgi/4233730‑4233986                                                                                                                |                                                                                                                                                  ttcgaagttcctttactttttagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                              <  1:114‑M2/38‑1 (MQ=255) ttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                              >  2:515218‑M2/112‑149 (MQ=255)        ttcctatactttctagtgaataggaacttctaactgcaggtcgacgggtccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                       <  1:596596‑M2/45‑1 (MQ=255)           ctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                    >  1:465993‑M2/102‑149 (MQ=255)              tactttctagagaataggattttcgttctgtaggtcgttggttccgcgggctgccgtaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAc                                                                                                 <  1:18614‑M2/51‑1 (MQ=255)               actttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAcc                                                                                                <  1:360156‑M2/52‑1 (MQ=255)                    ctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc                                                                                           <  2:452643‑M2/57‑1 (MQ=255)                       gagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                    >  2:617608‑M2/90‑137 (MQ=255)                       gagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                                    <  1:617608‑M2/48‑1 (MQ=255)                       gagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTg                                                                                        >  1:523035‑M2/90‑149 (MQ=255)                            taggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCg                                                                                   >  2:385881‑M2/85‑149 (MQ=255)                            taggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCg                                                                                   <  1:578897‑M2/65‑1 (MQ=255)                               gaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAc                                                                                 >  1:25488‑M2/82‑148 (MQ=255)                               gaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAc                                                                                 <  2:25488‑M2/67‑1 (MQ=255)                                         ctgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTc                                                                      <  1:542424‑M2/78‑1 (MQ=255)                                         ctgcaggtagacgcatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTc                                                                      <  1:542420‑M2/78‑1 (MQ=255)                                              ggtcgacgggtccccggaatgctcgaccaatggtctgattaacctctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                                 <  2:344900‑M2/83‑1 (MQ=255)                                               gtggacgtagccccggaatgctcgaccaatgtttggattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa                                                                <  2:183599‑M2/84‑1 (MQ=255)                                               gtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc                                                                                           <  1:539801‑M2/57‑1 (MQ=255)                                               gtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc                                                                                           >  2:539801‑M2/66‑122 (MQ=255)                                                        tccccggaatgctcgaccactggtctgattaactgctataaagagtggcgcggttaATCATCGTCGATATGTATGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGGCTGATGCGACGGGGTCGCGTCTTATCAGGCCTAAAg                                                       >  2:494292‑M2/57‑149 (MQ=255)                                                                      taccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGatat                                         >  2:570034‑M2/43‑149 (MQ=255)                                                                      gaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGatat                                         <  1:30809‑M2/107‑1 (MQ=255)                                                                          aatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTCCAGGTCGATGCCGATAGGTAc                                     >  2:174630‑M2/39‑149 (MQ=255)                                                                                 tgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAt                              >  1:501140‑M2/32‑149 (MQ=255)                                                                                   attaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc                            >  1:532899‑M2/30‑149 (MQ=255)                                                                                        ccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCaa                       <  1:385881‑M2/125‑1 (MQ=255)                                                                                        ccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCaa                       <  2:60760‑M2/125‑1 (MQ=255)                                                                                         cgctataaagcgtggcgcggttaATCATCGTCGATATGTAGCCCGGATAAGGCGGTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGATCGATGCCGATATGTACATCGTATTCGGCAAt                      >  2:630422‑M2/24‑149 (MQ=255)                                                                                          gctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt                     <  1:426036‑M2/127‑1 (MQ=255)                                                                                            tataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTaa                   >  1:103514‑M2/21‑149 (MQ=255)                                                                                                   cggggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTAAATGGCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAg            <  1:570034‑M2/136‑1 (MQ=255)                                                                                                     tggcgcggttaATTAGCGTCGAGATGTATGCCGGATAAGGGGTTCACGCCGCATCCGGCAACCGATGCCGGATGCGACGCGGTCGCGGCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCa          <  2:591004‑M2/138‑1 (MQ=255)                                                                                                            gttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGCTATGTCCATCGTATTCGGCAATTAATACATAGCACgattga   >  1:119724‑M2/5‑149 (MQ=255)                                                                                                             ttaATCATCGTCGCTATGTATGCCGGATAAGGCGTTCACGGCCGGTTCGGCAACCGATGCCTGTTGCCACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattgat  >  2:137587‑M2/4‑149 (MQ=255)                                                                                                             ttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattgat  >  2:328397‑M2/4‑149 (MQ=255)                                                                                                             ttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattgat  <  2:103514‑M2/146‑1 (MQ=255)                                                                                                                |                                                                                                                                                  AATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGAT  >  NC_000913_3_KO_pgi/4233730‑4233986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.