Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_KO_pgi 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_KO_pgi 1299499–1300695 1300697 3–1199 57 [0] [0] 57 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 12994980 (0.000)56 (1.130) 46/278 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_KO_pgi 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

CTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCC  >  NC_000913_3_KO_pgi/1299353‑1299644
                                                                                                                                                 |                                                                                                                                                  
ctcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAata                                                                                                                                                 >  2:283749‑M1/1‑146 (MQ=255)
 tcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                                                                                                                                >  1:123776‑M1/1‑145 (MQ=255)
   aaaTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataata                                                                                                                                              <  1:703950‑M1/149‑7 (MQ=255)
    aaTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGATGAAGGATAGGGGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataa                                                                                                                                             >  1:433813‑M1/1‑142 (MQ=255)
         cAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaat                                                                                                                                        >  2:905468‑M1/1‑137 (MQ=255)
            ttagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcatttgt                                                                                                                                     >  1:135136‑M1/1‑134 (MQ=255)
                    aaGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGATCAataataaccactttttaatcaat                                                                                                                             >  2:398966‑M1/1‑126 (MQ=255)
                        aTTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATGATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTACTACaaaataatcaactgttaaattattgtg                                                                                                                         >  2:657965‑M1/1‑122 (MQ=255)
                             cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                                    >  1:853542‑M1/1‑117 (MQ=255)
                                  cTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGTAAAGTGCTAAataataatcaatttttaatttattgtacatttcccta                                                                                                               >  2:426786‑M1/1‑112 (MQ=255)
                                  cTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta                                                                                                               <  1:738509‑M1/149‑38 (MQ=255)
                                    gCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactact                                                                                                             <  1:283749‑M1/149‑40 (MQ=255)
                                      ttCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGGGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcaatactgg                                                                                                           >  1:597381‑M1/1‑108 (MQ=255)
                                      ttCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                                                                           >  1:515685‑M1/1‑108 (MQ=255)
                                         tcctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaac                                                                                                        <  2:123776‑M1/149‑45 (MQ=255)
                                           ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaacag                                                                                                      >  1:184359‑M1/1‑103 (MQ=255)
                                            tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgt                                                                                                     <  2:322775‑M1/149‑48 (MQ=255)
                                              aCCATCCTGTTATTTTCGCCACATCATAACCCTGGGCTTTCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTTGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaa                                                                                                   <  2:480982‑M1/149‑50 (MQ=255)
                                              aCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatttttcatttcattactggaactttaa                                                                                                   >  1:32952‑M1/1‑100 (MQ=255)
                                                     tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattattcaattattgtgcatttcactactggaactgtaatcaggaa                                                                                            >  1:165428‑M1/1‑93 (MQ=255)
                                                      gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                           <  1:272570‑M1/149‑58 (MQ=255)
                                                      gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacggcttgaactgtaatcagaaaa                                                                                           <  2:878697‑M1/149‑58 (MQ=255)
                                                       ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaag                                                                                          >  2:573221‑M1/1‑91 (MQ=255)
                                                            ttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                                     <  1:759506‑M1/149‑64 (MQ=255)
                                                                       atAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagcc                                                                          >  1:140616‑M1/1‑75 (MQ=255)
                                                                           tCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatc                                                                      >  2:179575‑M1/1‑71 (MQ=255)
                                                                              ttggctTGCTGAAGAATTAGTGAAATTATATTATTAATTCCACTGCCTTTGGTAGAGGAAATTGCTAAtttataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagcctttcttagccaatctct                                                                   <  2:335002‑M1/147‑82 (MQ=255)
                                                                              tGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctct                                                                   <  2:335004‑M1/149‑82 (MQ=255)
                                                                                       tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                                                                             >  2:716206‑M1/1‑59 (MQ=255)
                                                                                       tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                                                                             <  1:716327‑M1/130‑72 (MQ=255)
                                                                                       tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                                                                             <  1:716206‑M1/130‑72 (MQ=255)
                                                                                       tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                                                                             >  2:716327‑M1/1‑59 (MQ=255)
                                                                                       tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGAGAGAGGAAAGTGCTGCaagagaataaattgttaaatcattatgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatatctatttgcttg                                                          >  2:871641‑M1/1‑59 (MQ=255)
                                                                                               aatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                                  <  2:433813‑M1/149‑99 (MQ=255)
                                                                                                 ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                           >  1:745283‑M1/1‑49 (MQ=255)
                                                                                                 ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                           <  2:745283‑M1/122‑74 (MQ=255)
                                                                                                     aaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagaaatgcttagccaatctctattttattgaatagaaagatgtt                                            >  2:220363‑M1/1‑45 (MQ=255)
                                                                                                      aTGATATTATTAATTCCACTGGCGTTTGGAGAGGAAAGTGTGAAataataatcaatttttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                           <  2:368910‑M1/149‑106 (MQ=255)
                                                                                                          tattatTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgtttagccaatctctatttgattgaattgaaaggtgtttgtta                                       >  2:765936‑M1/1‑40 (MQ=255)
                                                                                                                 aaTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccactataaacgccggttaattgaaagatgtttgttaaggcatg                                <  1:670435‑M1/149‑117 (MQ=255)
                                                                                                                    tCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaagtgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                             <  1:271114‑M1/149‑120 (MQ=255)
                                                                                                                     cccctggCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgcaactgtaatcagaaaagatagacaggcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatg                            <  1:372472‑M1/146‑121 (MQ=255)
                                                                                                                       aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaaaatgtttgttaagccatgaatgca                          >  2:15171‑M1/1‑27 (MQ=255)
                                                                                                                       aCTGCCTTTGGTAGAGGAAAGTGATAAataatgatcaattgtgaacttattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcc                          >  1:715291‑M1/1‑27 (MQ=255)
                                                                                                                           ccTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagct                      >  2:390649‑M1/1‑23 (MQ=255)
                                                                                                                             tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                    <  1:482447‑M1/149‑129 (MQ=255)
                                                                                                                             tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                    <  1:179575‑M1/149‑129 (MQ=255)
                                                                                                                             tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgggcatttcactactggaactgtaatccgaatagatagacatgcttagccaatcactatttgattaaattgaaagatgtttgttaaggcatggatgcaagctat                    >  2:512425‑M1/1‑21 (MQ=255)
                                                                                                                             tttGGTAGAGGAAAGTACTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat                    >  1:788678‑M1/1‑21 (MQ=255)
                                                                                                                              ttGGTAGAGGAAAGTGCTAAatactaacgccattgtgtgttattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagacatggatgaaagctata                   >  2:420187‑M1/1‑20 (MQ=255)
                                                                                                                                ggTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctataga                 >  1:75086‑M1/1‑18 (MQ=255)
                                                                                                                                ggTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctataga                 <  1:190649‑M1/149‑132 (MQ=255)
                                                                                                                                  tAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                                  >  2:417043‑M1/1‑16 (MQ=255)
                                                                                                                                  tAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                                  <  1:417043‑M1/66‑51 (MQ=255)
                                                                                                                                       gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgat          >  1:145946‑M1/1‑11 (MQ=255)
                                                                                                                                       gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaagtgaaagatgtttgttaaggcatggatgcaagctatagattctgat          >  1:344610‑M1/1‑11 (MQ=255)
                                                                                                                                               tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaa  <  2:593979‑M1/149‑147 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCC  >  NC_000913_3_KO_pgi/1299353‑1299644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: