Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 948,983 A→T Y196N (TAT→AAT)  ycaN ← LysR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi948,9830AT98.1% 163.4 / NA 53Y196N (TAT→AAT) ycaNLysR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (25/27);  minor base G (1/0);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATAACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCTTTTTCGA  >  NC_000913_3_KO_pgi/948837‑949120
                                                                                                                                                  |                                                                                                                                         
gATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTAc                                                                                                                                         <  1:459983/149‑1 (MQ=255)
 aTCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTGCATCAGCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCGGGGATTACg                                                                                                                                        >  1:480652/1‑149 (MQ=255)
   cTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGaa                                                                                                                                      >  2:485050/1‑149 (MQ=255)
   cTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGaa                                                                                                                                      >  1:798867/1‑149 (MQ=255)
            gagCAGACACGCGATACCGGCTACCATCAAGGCGGCCTCCAGTTCACCATCAACATCATCCAGAATGCTATCTCCTGCGACCGCAATTTCCAGCGCCTTTGCAACTTGCCAGTTGAAATGTTTGCCGCGGGGATGCCGAAAcaccacgc                                                                                                                             >  2:83957/1‑147 (MQ=255)
                 gATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAACAACAACACATtgt                                                                                                                        >  2:625930/1‑148 (MQ=255)
                  aTACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATtgat                                                                                                                       >  1:97046/1‑149 (MQ=255)
                  aTACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTGGCCGCTGGGATTACGAAAAACAACACATtgat                                                                                                                       >  1:813499/1‑149 (MQ=255)
                      ccGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGAtt                                                                                                                   >  2:280960/1‑149 (MQ=255)
                             cAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGa                                                                                                            >  1:202141/1‑149 (MQ=255)
                               gCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAATACAACACATTGATGATTAAGCAGATc                                                                                                          <  1:485050/149‑1 (MQ=255)
                                   ccATCAAAACGGACGCGTGTTCAGCATCAATATCATCCAGAATGATATTTCCTGCGACAGCAATCTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGa                                                                                                      <  2:289075/149‑1 (MQ=255)
                                        aaaaCGGCCTCCAGAACAGCATCAAAAACATCAAAAATGAGATGTCGTGCGAGATCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGCATg                                                                                                 >  2:669194/1‑149 (MQ=255)
                                          aaCGGCCTCCAGTTCAGCCTCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAACCACATCATCAgcacacc                                                                                               >  2:446947/1‑143 (MQ=255)
                                            cgcccTCTACTTCAGCATCAACACCATCTATATTGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCgg                                                                                             <  2:363909/146‑1 (MQ=255)
                                            cGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCgg                                                                                             <  2:366753/149‑1 (MQ=255)
                                                cTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGACGATCATGAGGATGGCggggat                                                                                         >  2:416660/1‑147 (MQ=255)
                                                 tCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACTACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGttt                                                                                        >  2:567322/1‑149 (MQ=255)
                                                 tCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGttt                                                                                        >  1:584886/1‑149 (MQ=255)
                                                   cAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGc                                                                                      <  1:541546/149‑1 (MQ=255)
                                                     gTTCACCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCa                                                                                    <  1:362612/149‑1 (MQ=255)
                                                      ttCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCat                                                                                   >  1:733671/1‑149 (MQ=255)
                                                            aTCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGAtt                                                                                                                   <  1:729957/111‑1 (MQ=255)
                                                            aTCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGAtt                                                                                                                   >  2:729957/1‑111 (MQ=255)
                                                                aCATCATCCAGAATGATAGTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCaaa                                                                         <  2:131905/149‑1 (MQ=255)
                                                                aCATCAAACAGAAGGATATTTCCTGCTATAGCAATTTCCATGTCCTTTGCAAATTGCCAGTGGAAAGGTTTGACGAAAGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCaaa                                                                         <  2:734536/149‑1 (MQ=255)
                                                                      tcCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTActc                                                                   >  1:726892/1‑149 (MQ=255)
                                                                      tcCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTActc                                                                   <  2:584886/149‑1 (MQ=255)
                                                                      tcCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGAAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAAATCATGAGGATGGCGGGGTTTGCCATAGCTTGCACAGTActc                                                                   >  1:784194/1‑149 (MQ=255)
                                                                        cAGAATGATATTCCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTg                                                                 <  1:17767/149‑1 (MQ=255)
                                                                          gAATGATATTTCCTGCGACAGCAATTTCCAGCTCCATTGCTACTGGCCAGTGGAAAGGTTCGCCGCTGGGATTACGAAAAACAACACATTGATCATTAAGCAGATCATGAGGATCGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGc                                                               <  2:714099/149‑1 (MQ=255)
                                                                             tGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATAAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTATGGCGtt                                                            >  2:249764/1‑149 (MQ=255)
                                                                                     ccTGCGACAGCAATTTCCAGCTCCTTGGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGCTCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGTTGCTac                                                    <  1:468330/149‑1 (MQ=255)
                                                                                        gCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAGAGTACTCTGGCGTTACTGCTAcaca                                                 >  1:448921/1‑149 (MQ=255)
                                                                                         cGACAGCAATTTCCAGCTCCTTGGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACAt                                                <  1:857616/149‑1 (MQ=255)
                                                                                          gACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATa                                               >  2:624683/1‑149 (MQ=255)
                                                                                           aCATCAATTTCCAGTTCCTTTGCAAATTGCCCGTGGAAAGGTTTGTCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATaa                                              <  2:145736/149‑1 (MQ=255)
                                                                                            cAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAAt                                             <  1:280960/149‑1 (MQ=255)
                                                                                              gCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAAttt                                           >  1:732205/1‑149 (MQ=255)
                                                                                                  tttCCAGCTCCTTTGCAAAATGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACt                                       <  2:195478/149‑1 (MQ=255)
                                                                                                        gCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCggg                                 <  2:775652/149‑1 (MQ=255)
                                                                                                                gCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGa                                                                                                            <  2:98224/66‑1 (MQ=255)
                                                                                                                gCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGa                                                                                                            >  1:98224/1‑66 (MQ=255)
                                                                                                                 cAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGcc                        >  2:41650/1‑149 (MQ=255)
                                                                                                                         cAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGTGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTgct                >  1:361388/1‑147 (MQ=255)
                                                                                                                         cAGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGAt                <  2:388264/149‑1 (MQ=255)
                                                                                                                          aGTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATc               <  1:199063/149‑1 (MQ=255)
                                                                                                                           gTGGAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCa              <  1:859576/149‑1 (MQ=255)
                                                                                                                              gAAAGGTTTGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCatat           <  1:179813/149‑1 (MQ=255)
                                                                                                                               aaaGGTTTGCCGCTGGGAATACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATc          <  1:721590/149‑1 (MQ=255)
                                                                                                                                      tGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTtgctg                                                        >  2:332643/1‑96 (MQ=255)
                                                                                                                                      tGCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTtgctg                                                        <  1:332643/96‑1 (MQ=255)
                                                                                                                                       gCCGCTGGGATTACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCTTTTTcga  <  1:421455/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                         
GATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATATTTCCTGCGACAGCAATTTCCAGCTCCTTTGCAAATTGCCAGTGGAAAGGTTTGCCGCTGGGATAACGAAAAACAACACATTGATGATTAAGCAGATCATGAGGATGGCGGGGTTTGCCATATCTTGCAAAGTACTCTGGCGTTGCTGCTACACATAATTTCACTGGCGGGCCGATCGCCACGCTGATCATATCTTTTTCGA  >  NC_000913_3_KO_pgi/948837‑949120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑