Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 3,440,137 T→G K298T (AAA→ACA)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi3,440,1370TG98.4% 196.9 / NA 62K298T (AAA→ACA) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (28/33);  minor base C (1/0);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATC  >  NC_000913_3_KO_pgi/3439994‑3440282
                                                                                                                                               |                                                                                                                                                 
gcgcATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTa                                                                                                                                              <  1:832308/149‑1 (MQ=255)
gcgcATGACCTTATCCTCCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTa                                                                                                                                              <  1:858202/149‑1 (MQ=255)
 cgcATGACCTTATCCTTCTCAGTACAACCTTAACCTGTGATCCGGTTACTCGCCAGCGATGCTTGCCGGTAGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTAc                                                                                                                                             <  1:835109/149‑1 (MQ=255)
  gcATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAACCTCAGTAAGAGATGTTTTAcc                                                                                                                                            >  1:498770/1‑149 (MQ=255)
   cATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTAATCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCCGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGCCTTTATAACCAAGAAGAGATGTTTTACCa                                                                                                                                           >  2:223791/1‑149 (MQ=255)
          ttATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAg                                                                                                                                    >  1:408897/1‑149 (MQ=255)
               cTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCCGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGtt                                                                                                                               >  1:312326/1‑149 (MQ=255)
                ttCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGttt                                                                                                                              >  1:868589/1‑149 (MQ=255)
                    cAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAg                                                                                                                          <  2:134613/149‑1 (MQ=255)
                    cAGTAAAACCTTAACCTGTGATCCGGTTACGCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGAGAGTCCACGGGAAGCCAGGACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAg                                                                                                                          <  2:147963/149‑1 (MQ=255)
                      gTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGa                                                                                                                        >  2:417077/1‑149 (MQ=255)
                       tAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGGAAGAGTTGGTTTACCAAGGTTAGGCGTTTTAAGGAg                                                                                                                       >  1:701170/1‑149 (MQ=255)
                           aCCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCtga                                                                                                                   >  1:160908/1‑147 (MQ=255)
                                agccTGCGATCCGGTTACTCGCGAGCGATGCTTGCCGGTGGCCAGGTTTCCAGGCGCATGCCCAGAGACAGTTCACGGGAAGCCAGCACGTCTTTAATCTCGGTAAGAGATGTTTTACCAATGTTAGGCGTTTTAAGGAGCTCAACCTc                                                                                                              <  2:366474/147‑1 (MQ=255)
                                 cccggtgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                                                                             <  1:160238/145‑1 (MQ=255)
                                 aCCTTTGAGCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                                                                             <  1:160157/149‑1 (MQ=255)
                                 aCCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                                                                             <  2:900383/149‑1 (MQ=255)
                                   cTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTGTTACCCAGGTTAGGCGTTTTAAGGAGCGCCACCTccga                                                                                                           >  2:165326/1‑146 (MQ=255)
                                   cTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCACATGCCGGGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCggc                                                                                                           >  1:528529/1‑148 (MQ=255)
                                        aTCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTACGGAGATCAACCTCGGTACGCt                                                                                                      >  2:317216/1‑149 (MQ=255)
                                              ttACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCa                                                                                                >  2:49242/1‑149 (MQ=255)
                                                 ctggTCAGCGATGCTTGCCGGTGGCCAGTGTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGAt                                                                                             <  2:504187/146‑1 (MQ=255)
                                                 cTCGTCAGCGAGGTTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTACGAGATGTTTTACCAAGGTTAGTCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGAt                                                                                             <  1:317216/149‑1 (MQ=255)
                                                  tCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATc                                                                                            >  1:899585/1‑149 (MQ=255)
                                                  tCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCCCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATc                                                                                            <  1:55832/149‑1 (MQ=255)
                                                     tCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGTGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCATCCTCGGTACGCTGTACCAGATCAcc                                                                                         <  2:905219/149‑1 (MQ=255)
                                                     tCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCCCAGTAAGAGATCTTTTGCCAAGGATAGGAGGTTTAAGCACCTCAACCTCGGTACGCTGTGCCAGCTCAcc                                                                                         >  2:566413/1‑149 (MQ=255)
                                                             gCTTGCTGGTGTCCAGTTTTCCAGGCGCATGCTCAGAGGCAGTTCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGCGATGGTTTACCAAGGTTAGGCGTTTTAAGGAGCGCAACCTCGGTACGCTGTACCAGATCACCGATATAGt                                                                                 <  1:436089/149‑1 (MQ=255)
                                                             gCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGt                                                                                 >  1:586141/1‑149 (MQ=255)
                                                               ttGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTgg                                                                               >  2:279588/1‑149 (MQ=255)
                                                                tGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGa                                                                              <  2:586141/149‑1 (MQ=255)
                                                                 gCCGGGGGCCGGTTTGCCAGGGGGATTCTCAGAGACAGTCCATGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTTCCAAGGTTAGGCTTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGAt                                                                             <  2:223810/149‑1 (MQ=255)
                                                                      tGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTCGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCtt                                                                        <  1:30327/149‑1 (MQ=255)
                                                                         ccAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGGTTTAAGGAGCTCAACCGCGGTACGCTGTACCAGAGCGCCAAGATAATGGATAGCTtgtg                                                                     >  2:163240/1‑149 (MQ=255)
                                                                          cAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGc                                                                    >  1:517058/1‑149 (MQ=255)
                                                                               ttCGAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTaa                                                               <  2:563201/149‑1 (MQ=255)
                                                                               ttCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGATGTAAAATAGCACCGATATAGTGGATAGCTTCTGCTTTaa                                                               <  1:670403/149‑1 (MQ=255)
                                                                                 ccAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAgg                                                             <  1:338601/149‑1 (MQ=255)
                                                                                  cAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGc                                                            >  2:528049/1‑149 (MQ=255)
                                                                                   aGGCGCATGCCGAGAAACAGTGCACGAGAAGCCAGCAAGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCTGGACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCa                                                           <  2:610226/149‑1 (MQ=255)
                                                                                   aGGCGCATGCCCAGGGACAGTCCACGGGAAGCCAGCACTTCTTTAATCTCAGTAAGATATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCa                                                           <  2:339909/149‑1 (MQ=255)
                                                                                       gcATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTa                                                       <  1:638045/149‑1 (MQ=255)
                                                                                        cATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGCCCCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAg                                                      <  1:809526/149‑1 (MQ=255)
                                                                                            cccAGAGACAGGCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCaga                                                  <  1:581378/149‑1 (MQ=255)
                                                                                              cAGAGACAGTCCACGGGAAGCAAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATAACCGATCTAGTGGGTAGCTTCTGCTTTAACGCAGTTaaccgatc                                                >  2:785366/1‑142 (MQ=255)
                                                                                                 agaCAGTCCACGGGACTCCAGCTCGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGa                                             <  2:660421/149‑1 (MQ=255)
                                                                                                   aCAGTCGACGGGAAGCCAGCACGTCTTTAATCGCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACa                                           <  2:91160/149‑1 (MQ=255)
                                                                                                     aGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGCCAGt                                         >  2:521175/1‑149 (MQ=255)
                                                                                                      gTCCACGGGAAGCCAGCACGTCTTTAACCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTc                                        <  2:498770/149‑1 (MQ=255)
                                                                                                       tCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCa                                       >  2:263951/1‑149 (MQ=255)
                                                                                                                 gCCAGCACGTCTTTAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTGTGAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATc                             <  1:359789/149‑1 (MQ=255)
                                                                                                                            tttAATCTCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGACGTTAGCAGCGCGGACAGTCAATTCCAGATCGTCAACACGgc                  >  1:390758/1‑149 (MQ=255)
                                                                                                                                aTCCCAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGATCTCAACCTCGGTACGCTGTACCAGATCACCGACATAGTGGAAAGCTTCTGCTTTCAGGCAGCTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCgcag              <  1:212048/149‑1 (MQ=255)
                                                                                                                                 tctcAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGgcg                 <  2:441997/145‑1 (MQ=255)
                                                                                                                                 tctcAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGgcg                 >  1:441997/1‑145 (MQ=255)
                                                                                                                                 tctcAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACACTCAACTCCCAATCGCGAACCGGgcgctcc             >  1:883658/1‑146 (MQ=255)
                                                                                                                                   tcAGTAAGGGATGGTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGcagcag           <  1:377022/149‑1 (MQ=255)
                                                                                                                                   tcAGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTGCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGcagcag           >  1:663423/1‑149 (MQ=255)
                                                                                                                                     aGTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTAACAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAAAGCGGGCAGTCAATTCCAGCTCGTCAAGCGGGCGCAGCAggc         >  2:884436/1‑148 (MQ=255)
                                                                                                                                      gTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCagag                                                 >  2:426115/1‑108 (MQ=255)
                                                                                                                                      gTAAGAGATGTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCagag                                                 <  1:426115/108‑1 (MQ=255)
                                                                                                                                            gaTGTTTTACGAATTTTTGGCGGTTTAAGTAGCTCAACCTCTGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAggatcggatc  >  2:670338/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCAGGATCGGATC  >  NC_000913_3_KO_pgi/3439994‑3440282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: