Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi460,1460TC25.2% 170.1 / 64.2 99F420S (TTC→TCC) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base T (41/33);  new base C (11/14);  total (52/47)
Fisher's exact test for biased strand distribution p-value = 3.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 101 total aligned reads displayed.

CAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTC  >  NC_000913_3_pae_pgi/459997‑460294
                                                                                                                                                     |                                                                                                                                                    
cAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGtt                                                                                                                                                      >  2:1144954/1‑150 (MQ=255)
    gccgcggggCGTAAATATGTCCGTACGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGGCGGGTAAACTGTTCCAGAAAATGTCGAAAATGGAAAAAAAAAACCCGCTGTTCCTg                                                                                                                                                  <  2:831388/144‑1 (MQ=255)
     ccACCGGGCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCt                                                                                                                                                   >  3:106605/1‑148 (MQ=255)
     ccACCGGGCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCt                                                                                                                                                   <  4:106605/148‑1 (MQ=255)
      cACCGGGCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCt                                                                                                                                                <  1:744798/150‑1 (MQ=255)
          gggCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGAt                                                                                                                                            <  4:56027/150‑1 (MQ=255)
            gCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAGAATGGCGAGAGTGGGCGTGAAAAACCGGCTGTTCCTGCTCGATga                                                                                                                                          >  2:944709/1‑150 (MQ=255)
            gCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATga                                                                                                                                          <  1:895861/150‑1 (MQ=255)
            gCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATga                                                                                                                                          <  3:181946/150‑1 (MQ=255)
            gCGTAAATATGTCCGTAAGGTGCGGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATga                                                                                                                                          <  3:571728/150‑1 (MQ=255)
              gTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATgaga                                                                                                                                        <  1:396639/150‑1 (MQ=255)
               tAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGAt                                                                                                                                       >  4:466537/1‑150 (MQ=255)
                 aaTATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCg                                                                                                                                     <  1:265785/150‑1 (MQ=255)
                 aaTATGTCCGCATGGCGCTGGTCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGATAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCg                                                                                                                                     <  3:136615/150‑1 (MQ=255)
                     tGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACaa                                                                                                                                 <  3:173245/150‑1 (MQ=255)
                        ccGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAAt                                                                                                                              >  4:205743/1‑150 (MQ=255)
                         cGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTc                                                                                                                                                     <  1:1034353/126‑1 (MQ=255)
                         cGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTc                                                                                                                                                     >  2:1034353/1‑126 (MQ=255)
                                cgcTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTg                                                                                                                      <  4:125149/150‑1 (MQ=255)
                                 gcTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGa                                                                                                                     >  2:640278/1‑150 (MQ=255)
                                   tGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACAACGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACa                                                                                                                   <  2:152560/150‑1 (MQ=255)
                                     ggcggcGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCt                                                                                                                                                <  3:186158/119‑1 (MQ=255)
                                     ggcggcGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCt                                                                                                                                                >  4:186158/1‑119 (MQ=255)
                                      gcggcgTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGc                                                                                                                <  1:1144954/150‑1 (MQ=255)
                                          cgtgcgtgATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGCTCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGATCCTGCTCGATGAGAGCGACAAAATGTCTTCTGACATGCGtcg                                                                                                            >  2:859874/1‑148 (MQ=255)
                                          cgtgcgtgATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTgg                                                                                                            >  2:859941/1‑150 (MQ=255)
                                          cgtgcgtgATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTgg                                                                                                            >  4:67380/1‑150 (MQ=255)
                                              cgtgATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGAt                                                                                                        >  4:156002/1‑150 (MQ=255)
                                                  atgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCgg                                                                                                    >  2:1003546/1‑150 (MQ=255)
                                                  atgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCgg                                                                                                    >  4:212348/1‑150 (MQ=255)
                                                  atgaAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCgg                                                                                                    >  2:363150/1‑150 (MQ=255)
                                                     aaGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCct                                                                                                 >  3:263038/1‑150 (MQ=255)
                                                       gCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCctct                                                                                               >  3:584672/1‑150 (MQ=255)
                                                         ggAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGc                                                                                             <  1:1157390/150‑1 (MQ=255)
                                                         ggAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGc                                                                                             >  1:329518/1‑150 (MQ=255)
                                                          gAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCa                                                                                            >  2:390990/1‑150 (MQ=255)
                                                           aaaTCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATGGACAAAATGTCATCTGACATGCGTGGCGATCCGGCCTCTGCAc                                                                                           >  2:912055/1‑150 (MQ=255)
                                                                        ccgccgTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAATCCCGCTGTTCCTGCTCGATGAGATCGACAAAAGGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCt                                                                              <  2:123851/150‑1 (MQ=255)
                                                                            cgTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGAt                                                                          <  1:905827/150‑1 (MQ=255)
                                                                               aCTTACCTCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCa                                                                       <  4:602285/150‑1 (MQ=255)
                                                                               aCTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCa                                                                       >  3:429466/1‑150 (MQ=255)
                                                                               aCTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCa                                                                       <  1:653564/150‑1 (MQ=255)
                                                                                cTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACaaaa                                                                                                                               <  2:501515/93‑1 (MQ=255)
                                                                                cTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACaaaa                                                                                                                               >  1:501515/1‑93 (MQ=255)
                                                                                cTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCag                                                                      >  3:164525/1‑150 (MQ=255)
                                                                                 ttACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGAt                                                                                                                                            >  3:293115/1‑79 (MQ=255)
                                                                                 ttACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGAt                                                                                                                                            <  4:574691/79‑1 (MQ=255)
                                                                                 ttACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGAt                                                                                                                                            <  4:293115/79‑1 (MQ=255)
                                                                                 ttACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGAt                                                                                                                                            >  3:574691/1‑79 (MQ=255)
                                                                                 ttACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCaga                                                                     >  4:701226/1‑150 (MQ=255)
                                                                                  tACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCagag                                                                    <  2:150216/150‑1 (MQ=255)
                                                                                  tACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCagag                                                                    <  3:602128/150‑1 (MQ=255)
                                                                                   aCATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATgaga                                                                                                                                        >  4:417288/1‑81 (MQ=255)
                                                                                   aCATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATgaga                                                                                                                                        <  3:417288/81‑1 (MQ=255)
                                                                                   aCATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGc                                                                   >  2:95227/1‑150 (MQ=255)
                                                                                    cATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCa                                                                  <  3:462203/150‑1 (MQ=255)
                                                                                     aTCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCg                                                                                                                                              <  2:259352/73‑1 (MQ=255)
                                                                                     aTCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCg                                                                                                                                              >  1:259352/1‑73 (MQ=255)
                                                                                     aTCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAg                                                                 >  2:457461/1‑150 (MQ=255)
                                                                                      tCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGa                                                                >  1:293685/1‑150 (MQ=255)
                                                                                             tATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGc                                                         >  2:166165/1‑150 (MQ=255)
                                                                                             tATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGc                                                         >  2:864695/1‑150 (MQ=255)
                                                                                             tATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGc                                                         >  3:281220/1‑150 (MQ=255)
                                                                                              aTGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCg                                                        <  4:346555/150‑1 (MQ=255)
                                                                                                gCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGtt                                                      <  1:640278/150‑1 (MQ=255)
                                                                                                  cGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGCGCAGAACGTAGCGTTcc                                                    >  2:372073/1‑149 (MQ=255)
                                                                                                    ggTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGc                                                  <  1:95227/150‑1 (MQ=255)
                                                                                                       aaaCTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTTCTTGTTCCAGAGCAGAACGTAGCGTTCAGCGAc                                               <  2:298453/150‑1 (MQ=255)
                                                                                                         aCTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCa                                             >  2:814549/1‑150 (MQ=255)
                                                                                                           tGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGtcttct                                                                                                                       <  2:775815/74‑1 (MQ=255)
                                                                                                           tGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGtcttct                                                                                                                       >  1:775815/1‑74 (MQ=255)
                                                                                                           tGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACt                                           >  4:594770/1‑150 (MQ=255)
                                                                                                           tGATCCAGAAAATGGCCAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACt                                           >  3:662467/1‑150 (MQ=255)
                                                                                                              tCCAGAAAATGGCGCAATGTTTCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTAcc                                        <  1:954375/150‑1 (MQ=255)
                                                                                                               ccAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCt                                       >  1:874862/1‑150 (MQ=255)
                                                                                                                cAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTg                                                                                              <  3:637095/94‑1 (MQ=255)
                                                                                                                cAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTg                                                                                              >  4:637095/1‑94 (MQ=255)
                                                                                                                cAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTg                                      <  3:240345/150‑1 (MQ=255)
                                                                                                                  gAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGa                                    >  1:2294/1‑150 (MQ=255)
                                                                                                                      aTGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTg                                >  4:181147/1‑150 (MQ=255)
                                                                                                                        ggCGAACGTGGGCGTGAACAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTCGCGATCCGGCCTCTGCACTGCTTGAAGTGATGAATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGACGtcaa                              <  2:93961/150‑4 (MQ=255)
                                                                                                                         gCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCt                                       <  1:868154/140‑1 (MQ=255)
                                                                                                                         gCGAAAGTGGGCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCt                                       >  2:868154/1‑140 (MQ=255)
                                                                                                                           gAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTa                           <  1:166165/150‑1 (MQ=255)
                                                                                                                            aaaGTTGGTGTGAAAAACCCGCTTTTCCTGCTTGATGAGATCGACAAATTGTCTTCTGTCATGCGTTGCGTTCCGGCCTTTGCACTGCTTGAAGTGCTGGTTCCAGAGCAGAACTTAGCGTTCTGCTACCACTACCTGGAAGTGGATTAc                          <  4:212560/150‑1 (MQ=255)
                                                                                                                                 gggCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGAtct                     >  1:1136775/1‑150 (MQ=255)
                                                                                                                                   gCGTGAAAAACCCGCTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCa                   >  4:279906/1‑150 (MQ=255)
                                                                                                                                     gTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGc                 <  4:584672/150‑1 (MQ=255)
                                                                                                                                       gAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGa               >  4:366959/1‑150 (MQ=255)
                                                                                                                                       gAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGa               >  4:517776/1‑150 (MQ=255)
                                                                                                                                         aaaaCCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACg             <  4:662467/150‑1 (MQ=255)
                                                                                                                                          aaaCCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGt            >  4:427218/1‑150 (MQ=255)
                                                                                                                                          aaaCCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGt            >  1:713504/1‑150 (MQ=255)
                                                                                                                                            aCCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGa          <  3:205743/150‑1 (MQ=255)
                                                                                                                                            aCCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGa          <  3:67380/150‑1 (MQ=255)
                                                                                                                                              ccGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGAc                                               <  2:310425/111‑1 (MQ=255)
                                                                                                                                              ccGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGAc                                               >  1:310425/1‑111 (MQ=255)
                                                                                                                                              ccGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATg        >  2:161892/1‑150 (MQ=255)
                                                                                                                                                 cTGTCCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGttt     <  3:362992/150‑1 (MQ=255)
                                                                                                                                                    ttCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGCTCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTc  <  1:462008/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAAAGCCACCGGGCGTAAATATGTCCGTATGGCGCTGGGCGGCGTGCGTGATGAAGCGGAAATCCGTGGTCACCGCCGTACTTACATCGGTTCTATGCCGGGTAAACTGATCCAGAAAATGGCGAAAGTGGGCGTGAAAAACCCGCTGTTCCTGCTCGATGAGATCGACAAAATGTCTTCTGACATGCGTGGCGATCCGGCCTCTGCACTGCTTGAAGTGCTGGATCCAGAGCAGAACGTAGCGTTCAGCGACCACTACCTGGAAGTGGATTACGATCTCAGCGACGTGATGTTTGTC  >  NC_000913_3_pae_pgi/459997‑460294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑