Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,707,4400GT21.7% 192.3 / 46.9 83E174D (GAG→GATrsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base G (31/34);  new base T (10/8);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 6.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGATTATCGCACTCGCAGTCGCGAAGAGTTAATCGAGCGCATACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCT  >  NC_000913_3_pae_pgi/1707293‑1707589
                                                                                                                                                   |                                                                                                                                                     
cGATTATCGCACTCGCAGTCGCGAAGAGTTAATCGAGCGCATACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTg                                                                                                                                                     >  3:127159/1‑150 (MQ=255)
cGATTATCGCACTCGCAGTCGCGAAGAGTTAATCGAGCGCATACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTg                                                                                                                                                     <  3:680002/150‑1 (MQ=255)
       cGCACTCGCAGTCGCGAAGAGTTAATCGAGCGCATACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCg                                                                                                                                              <  2:555353/150‑1 (MQ=255)
        gCACTCGCAGTCGCGAAGAGTTAATCGAGCGCATACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATGCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGAGAACATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGt                                                                                                                                             >  2:1147356/1‑150 (MQ=255)
         cACTCGCAGTCGCGAAGAGTTAATCGAGCGCATACATCAGTTTGGTGTTGCCCGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTa                                                                                                                                            >  2:781802/1‑150 (MQ=255)
              gCAGTCGCGAAGAGTTAATCGAGCGCATACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTa                                                                                                                                       <  4:144220/150‑1 (MQ=255)
                   cgcgAAGAGTTAATCGAGCGCATACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGGGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTAccgcc                                                                                                                                  <  3:391548/150‑1 (MQ=255)
                    gcgAAGAGTTAATCGAGCGCATACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCTGTAAATTGCAGGGTGGCGGAGAGAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTAccgccg                                                                                                                                 >  3:524672/1‑150 (MQ=255)
                    gcgAAGAGTTAATCGAGCGCATACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGATTGCGAGCCGTACATTAccgccg                                                                                                                                 >  1:819394/1‑150 (MQ=255)
                            ttAATCGAGCGCATACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGt                                                                                                                         >  3:350498/1‑150 (MQ=255)
                             tAATCGAGCGCATACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGATTGCGAGCCGTACATTACCGCCGATGACCGtt                                                                                                                        <  2:819394/150‑1 (MQ=255)
                              aaTCGAGCGCATACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGATTGCGAGCCGTACATTACCGCCGATGACCGttt                                                                                                                       <  4:523284/150‑1 (MQ=255)
                               aTCGAGCGCATACATCAGTTTGGTGTTGCCCGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTg                                                                                                                      <  1:781802/150‑1 (MQ=255)
                                gcGAGCGCGTACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGAGAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGa                                                                                                                     <  4:668546/149‑1 (MQ=255)
                                  gAGCGCATACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATg                                                                                                                   >  2:288020/1‑150 (MQ=255)
                                  gAGCGCATACATCAGGTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGAGTATCAACGCGGCTGAGTGCGAGCCGGACATTACCGCCGATGACCGTTTGATg                                                                                                                   <  2:764842/150‑1 (MQ=255)
                                   aGCGCATACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGc                                                                                                                  >  4:576729/1‑150 (MQ=255)
                                         tACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGAtt                                                                                                            <  1:637685/150‑1 (MQ=255)
                                            aTCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTgcg                                                                                                         >  4:516741/1‑150 (MQ=255)
                                             tCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTgcgc                                                                                                        <  1:940391/150‑1 (MQ=255)
                                             tCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTgcgc                                                                                                        >  4:524994/1‑150 (MQ=255)
                                                    ggTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGATTGCGAGCCGTACATTAccg                                                                                                                                    >  1:800076/1‑115 (MQ=255)
                                                    ggTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGATTGCGAGCCGTACATTAccg                                                                                                                                    <  2:800076/115‑1 (MQ=255)
                                                     gtgtTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGATTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAgg                                                                                                <  4:6292/150‑1 (MQ=255)
                                                       gtTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTc                                                                                              <  4:237119/150‑1 (MQ=255)
                                                        ttGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTcc                                                                                             >  1:132407/1‑149 (MQ=255)
                                                          gCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTa                                                                                           <  4:653127/150‑1 (MQ=255)
                                                            cGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTaga                                                                                         >  1:663352/1‑150 (MQ=255)
                                                             gggCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTagag                                                                                        <  4:83028/150‑1 (MQ=255)
                                                              ggCTGGGCGGTGCAGGATTCCCGACAGGCGTTAACTTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAgg                                                                                       >  4:527518/1‑150 (MQ=255)
                                                              ggCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAgg                                                                                       <  1:288020/150‑1 (MQ=255)
                                                                 tGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTa                                                                                    >  1:286444/1‑150 (MQ=255)
                                                                 tGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTa                                                                                    >  2:734869/1‑150 (MQ=255)
                                                                   ggCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTAtt                                                                                  >  1:367335/1‑150 (MQ=255)
                                                                    gCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAg                                                                                                                >  1:1096364/1‑119 (MQ=255)
                                                                    gCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAg                                                                                                                <  2:1096364/119‑1 (MQ=255)
                                                                        tGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGATTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCAt                                                                             >  3:508283/1‑150 (MQ=255)
                                                                           aGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCt                                                                          >  3:623408/1‑150 (MQ=255)
                                                                            ggATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGATTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCtt                                                                         <  2:656646/150‑1 (MQ=255)
                                                                            ggATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCtt                                                                         <  3:527851/150‑1 (MQ=255)
                                                                              aTTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTgc                                                                       <  3:524994/150‑1 (MQ=255)
                                                                               ttCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTgcg                                                                      <  4:127159/150‑1 (MQ=255)
                                                                                     aCAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATAtt                                                                <  3:147092/150‑1 (MQ=255)
                                                                                         gCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGc                                                            <  2:663352/150‑1 (MQ=255)
                                                                                          cGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCa                                                           <  3:369700/150‑1 (MQ=255)
                                                                                            ttAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAgg                                                                                       >  1:513238/1‑120 (MQ=255)
                                                                                            ttAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAgg                                                                                       <  2:513238/120‑1 (MQ=255)
                                                                                              aaaTTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAgg                                                                                       <  1:337899/118‑1 (MQ=255)
                                                                                              aaaTTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAgg                                                                                       >  2:337899/1‑118 (MQ=255)
                                                                                              aaaTTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCa                                                       >  2:1129876/1‑150 (MQ=255)
                                                                                               aaTTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCAc                                                      <  2:132407/150‑1 (MQ=255)
                                                                                                    cAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGaa                                                 <  4:623408/150‑1 (MQ=255)
                                                                                                        gTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGATTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTc                                             <  4:542009/150‑1 (MQ=255)
                                                                                                         tGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGATTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCt                                            >  2:920114/1‑150 (MQ=255)
                                                                                                           gCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTa                                          <  3:720593/150‑1 (MQ=255)
                                                                                                            cGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTAt                                         >  3:354235/1‑150 (MQ=255)
                                                                                                            cGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTAt                                         >  3:354378/1‑150 (MQ=255)
                                                                                                             ggAGATAAGATTGAAACGTTGATTATCAACGCGGCTGATTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATc                                        >  1:277443/1‑150 (MQ=255)
                                                                                                             ggAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTc                                                                                 >  1:329331/1‑109 (MQ=255)
                                                                                                             ggAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTc                                                                                 <  2:329331/109‑1 (MQ=255)
                                                                                                              gagaTAAGATTGAAACGTTGATTATCAGCGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCg                                       <  2:506066/150‑1 (MQ=255)
                                                                                                              gagaTAAGATTGAAACGTTGATTATCAACGCGGCTGATTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCg                                       >  4:600704/1‑150 (MQ=255)
                                                                                                              gagaTAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCg                                       <  2:286444/150‑1 (MQ=255)
                                                                                                                  tAAGATTGAAACGTTGATTATCAACGCGGCTGATTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCAt                                   <  4:508283/150‑1 (MQ=255)
                                                                                                                   aaGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAgg                                                                                                <  1:981957/88‑1 (MQ=255)
                                                                                                                   aaGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAgg                                                                                                >  2:981957/1‑88 (MQ=255)
                                                                                                                    aGATTGAAACGTTGATTATCAACGCGGCTGATTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTg                                 >  2:1033166/1‑150 (MQ=255)
                                                                                                                       ttGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAg                              >  1:848569/1‑150 (MQ=255)
                                                                                                                        tGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTGTTATCGGCATTGAAGa                             <  3:663116/150‑1 (MQ=255)
                                                                                                                              gTTGATTATCAACGCGGCTGATTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAGATAACaa                       >  2:253948/1‑150 (MQ=255)
                                                                                                                              gTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTgc                                                                       >  1:859030/1‑102 (MQ=255)
                                                                                                                              gTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTgc                                                                       <  2:859030/102‑1 (MQ=255)
                                                                                                                               ttGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAGATAACaaa                      <  3:576729/150‑1 (MQ=255)
                                                                                                                                  aTTATCAACGCGGCTGATTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAGATAACAAACCg                   <  2:249164/150‑1 (MQ=255)
                                                                                                                                  aTTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGACGCAGAGGGTATTTGGAAACTCGAACCTACTCAACAGCATCGCCAAATTCTTATAGGCATCGAAGATAACAAACCg                   >  1:108452/1‑150 (MQ=255)
                                                                                                                                      tCAACGCGGCTGATTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAgg               >  1:726768/1‑150 (MQ=255)
                                                                                                                                      tCAACGCGGCTGAGCGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAgg               >  4:405463/1‑150 (MQ=255)
                                                                                                                                       catcgcgCCTGTGTGCGAGCCGTACATTACCGCCTATGTCCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCTCATATTCTGCCGCCACGCGAAATGCTAATCGGCATTGAAGATAACAAACCGCAGGc              <  2:944001/147‑1 (MQ=255)
                                                                                                                                        aaCGCGGCTGATTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCg             >  4:539265/1‑150 (MQ=255)
                                                                                                                                          cgcgGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGAt           >  3:107949/1‑150 (MQ=255)
                                                                                                                                           gcgGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGTAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGAtt          >  3:53784/1‑150 (MQ=255)
                                                                                                                                             ggCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTc                                             >  1:698708/1‑113 (MQ=255)
                                                                                                                                             ggCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTc                                             <  2:698708/113‑1 (MQ=255)
                                                                                                                                                   gTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCAtgct  >  4:276505/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGATTATCGCACTCGCAGTCGCGAAGAGTTAATCGAGCGCATACATCAGTTTGGTGTTGCCGGGCTGGGCGGTGCAGGATTCCCGACAGGCGTTAAATTGCAGGGTGGCGGAGATAAGATTGAAACGTTGATTATCAACGCGGCTGAGTGCGAGCCGTACATTACCGCCGATGACCGTTTGATGCAGGATTGCGCGGCTCAGGTCGTAGAGGGTATTCGCATTCTTGCGCATATTCTGCAGCCACGCGAAATTCTTATCGGCATTGAAGATAACAAACCGCAGGCGATTTCCATGCT  >  NC_000913_3_pae_pgi/1707293‑1707589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: