Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 1,195,102 T→G intergenic (‑151/‑21) rluE ← / → icd 23S rRNA pseudouridine(2457) synthase/isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,195,1020TG99.1% 407.4 / NA 116intergenic (‑151/‑21)rluE/icd23S rRNA pseudouridine(2457) synthase/isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (65/50);  minor base A (1/0);  total (66/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

TAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAA  >  NC_000913_3_pae_pgi/1194959‑1195246
                                                                                                                                               |                                                                                                                                                
taataaCGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCt                                                                                                                                            >  1:434567/1‑150 (MQ=255)
  ataaCGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTGTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCg                                                                                                                                          >  2:874301/1‑150 (MQ=255)
     aCGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTCACAAAAAATTGCTCTATAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAg                                                                                                                                       <  1:1010491/150‑1 (MQ=255)
     aCGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTTGTGGCAGACGAGCAAACCAGGAGCGCTCGAAg                                                                                                                                       <  2:646410/150‑1 (MQ=255)
      cgcgCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAgg                                                                                                                                      <  2:299201/150‑1 (MQ=255)
        cgcATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGgag                                                                                                                                    <  1:882850/150‑1 (MQ=255)
          cATCCTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGagag                                                                                                                                  <  3:691856/150‑1 (MQ=255)
           aTCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAgg                                                                                                                                 >  2:327618/1‑150 (MQ=255)
                     aCGGCAAACAATAGGGGAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGaaa                                                                                                                       <  4:649660/150‑1 (MQ=255)
                       ggCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGaaagt                                                                                                                     >  1:1017866/1‑150 (MQ=255)
                        gCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGaaagta                                                                                                                    >  3:626617/1‑150 (MQ=255)
                        gCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGaaagta                                                                                                                    >  2:1129061/1‑150 (MQ=255)
                          aaaCAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGaaagtaaa                                                                                                                  >  1:1099666/1‑150 (MQ=255)
                          aaaCAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGaaagtaaa                                                                                                                  <  1:581664/150‑1 (MQ=255)
                                tAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAgtt                                                                                                            <  2:51745/150‑1 (MQ=255)
                                tAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAgtt                                                                                                            <  2:1099666/150‑1 (MQ=255)
                                    gtagtaTTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAACGCATCCGTATCGCAGGACGCAAACGCATATGCAACGCGGTGGCAGACGCGCAAACCAGGAGCGCTCGAAGGCGCGGTGAACGGAAAGCACAGTAGTTGTTc                                                                                                        >  2:308589/1‑150 (MQ=255)
                                    gtaggaTTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCATGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCTCTCGAACGAGAGGTGAATGGAAAGTAAAGTAGTTGTTc                                                                                                        <  2:172857/150‑1 (MQ=255)
                                     tagtaTTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTcc                                                                                                       >  1:958318/1‑150 (MQ=255)
                                        taTTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACTCAAAAGCATATGCAACGTGGTGGCAGACGATCAAACCACAAGCGCTCGAAGGAGAGGTGAATGGAAAGTAGAGTAGTTGTTCCGGc                                                                                                    >  2:589331/1‑150 (MQ=255)
                                        taTTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGc                                                                                                    >  4:268049/1‑150 (MQ=255)
                                           tGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGcaca                                                                                                 >  2:355182/1‑150 (MQ=255)
                                            gACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGaa                                                                                                                             >  3:236631/1‑121 (MQ=255)
                                            gACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGaa                                                                                                                             <  4:236631/121‑1 (MQ=255)
                                                 gCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAgg                                                                                              <  3:170021/147‑1 (MQ=255)
                                                 gCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAgg                                                                                              >  4:170021/1‑147 (MQ=255)
                                                  ccAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCaag                                                                                          >  2:1092408/1‑150 (MQ=255)
                                                  ccAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCaag                                                                                          >  1:292955/1‑150 (MQ=255)
                                                   cAAGTACAAATCAATAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCaaga                                                                                         <  4:455374/150‑1 (MQ=255)
                                                    aaTTACAAATCATTCACAAAAAATTGCTCTAGAGCATGCGTATCGCAGGACGGAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCaagaa                                                                                        <  2:632686/150‑1 (MQ=255)
                                                         cAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCa                                                                                   <  3:476393/150‑1 (MQ=255)
                                                          aaaTCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGTGGAGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCAc                                                                                  >  4:411090/1‑150 (MQ=255)
                                                          aaaTCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGa                                                                                                                         >  2:322362/1‑111 (MQ=255)
                                                          aaaTCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGa                                                                                                                         <  1:322362/111‑1 (MQ=255)
                                                           aaTCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCAcc                                                                                 <  1:327618/150‑1 (MQ=255)
                                                           aaTCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCAcc                                                                                 >  2:56237/1‑150 (MQ=255)
                                                               aTTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGc                                                                             >  2:793890/1‑150 (MQ=255)
                                                                ttAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCa                                                                            >  2:1067519/1‑150 (MQ=255)
                                                                 tAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCaa                                                                           >  1:70448/1‑150 (MQ=255)
                                                                   aCAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCaaaa                                                                         <  2:593773/150‑1 (MQ=255)
                                                                   aCAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGAGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCaaaa                                                                         >  4:147725/1‑150 (MQ=255)
                                                                    cAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGCGGTGGCAGACGAGCAAACCAGGAGCGCCCGAAAGAGAGGAGAATGGAAAGTAACGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAAc                                                                        >  2:175449/1‑150 (MQ=255)
                                                                     aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACg                                                                       >  3:60917/1‑150 (MQ=255)
                                                                     aaaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACg                                                                       >  1:1075471/1‑150 (MQ=255)
                                                                      aaaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACgg                                                                      >  4:106505/1‑150 (MQ=255)
                                                                      aaaaaTTGCTCTAAAGCAACCGTATCGCAGGACGCAAACGCATATGCTACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGAGAATGGAAAGTAAAGCAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACgg                                                                      >  2:732423/1‑150 (MQ=255)
                                                                       aaaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGc                                                                     >  2:834977/1‑150 (MQ=255)
                                                                        aaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTcc                                                                                                       >  4:606722/1‑115 (MQ=255)
                                                                        aaaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTcc                                                                                                       <  3:606722/115‑1 (MQ=255)
                                                                         aaTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCaaaataccat                                                                   <  2:594141/150‑7 (MQ=255)
                                                                          aTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCaaa                                                                  >  1:964370/1‑150 (MQ=255)
                                                                          aTTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCaaa                                                                  >  3:725921/1‑150 (MQ=255)
                                                                                 tAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACg                                                           >  1:85423/1‑150 (MQ=255)
                                                                                  aaaGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTcc                                                                                                       <  3:340366/105‑1 (MQ=255)
                                                                                  aaaGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTcc                                                                                                       >  4:340366/1‑105 (MQ=255)
                                                                                    aGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTc                                                        <  4:60917/150‑1 (MQ=255)
                                                                                         ccGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGaa                                                   <  1:1129061/150‑1 (MQ=255)
                                                                                         ccGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGaa                                                   <  2:199059/150‑1 (MQ=255)
                                                                                              tCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATcc                                              >  2:213581/1‑150 (MQ=255)
                                                                                               cGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCg                                             >  4:548381/1‑150 (MQ=255)
                                                                                               cGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACAAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCg                                             <  2:1004201/150‑1 (MQ=255)
                                                                                                gCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGa                                            <  1:213581/150‑1 (MQ=255)
                                                                                                gCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGa                                            <  2:188882/150‑1 (MQ=255)
                                                                                                gCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGa                                            <  2:958318/150‑1 (MQ=255)
                                                                                                 cAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGAt                                           >  4:461197/1‑150 (MQ=255)
                                                                                                 cAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGAt                                           <  3:268049/150‑1 (MQ=255)
                                                                                                         aaaCGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCtt                                   >  3:450906/1‑150 (MQ=255)
                                                                                                         aaaCGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCtt                                   >  2:610109/1‑150 (MQ=255)
                                                                                                         aaaCGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCtt                                   >  2:342947/1‑150 (MQ=255)
                                                                                                          aaCGCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTa                                  <  2:82461/150‑1 (MQ=255)
                                                                                                             gCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACAt                               >  4:551125/1‑150 (MQ=255)
                                                                                                             gCATATGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACAt                               >  3:676925/1‑150 (MQ=255)
                                                                                                               atatGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTg                             >  2:225940/1‑150 (MQ=255)
                                                                                                                tatGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATGGa                            >  1:352443/1‑150 (MQ=255)
                                                                                                                 atGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTc                                                        >  2:1007007/1‑121 (MQ=255)
                                                                                                                 atGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTc                                                        <  1:1007007/121‑1 (MQ=255)
                                                                                                                 atGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGaa                           <  3:548381/150‑1 (MQ=255)
                                                                                                                  tGCAACGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAg                          >  2:836646/1‑150 (MQ=255)
                                                                                                                  tGCAACGTGGGGGCAGACGAGCAAACCATGAGCGCTGGAAGGAGAAGTAAAGGGAAATTAAAGGAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAg                          <  1:943034/150‑1 (MQ=255)
                                                                                                                     aaCGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGAt                                                                                     >  2:1165449/1‑88 (MQ=255)
                                                                                                                      aCGTGGTGGCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGa                      <  1:834977/150‑1 (MQ=255)
                                                                                                                            tggCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAAt                >  2:983800/1‑150 (MQ=255)
                                                                                                                            tggCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAAt                >  2:138067/1‑150 (MQ=255)
                                                                                                                             ggCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAAc                                                            >  2:910992/1‑105 (MQ=255)
                                                                                                                             ggCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAAc                                                            <  1:910992/105‑1 (MQ=255)
                                                                                                                             ggCAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGATCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATc               >  1:679434/1‑150 (MQ=255)
                                                                                                                               cAGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCgn             <  4:450906/150‑2 (MQ=255)
                                                                                                                                aGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGc                                                                     >  1:1152818/1‑93 (MQ=255)
                                                                                                                                aGACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGc                                                                     <  2:1152818/93‑1 (MQ=255)
                                                                                                                                 gACGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGgtg           >  2:800446/1‑150 (MQ=255)
                                                                                                                                  aCGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGgtgt          >  3:191636/1‑150 (MQ=255)
                                                                                                                                  aCGAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGgtgt          <  3:106505/150‑1 (MQ=255)
                                                                                                                                    gAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAg        >  2:628545/1‑150 (MQ=255)
                                                                                                                                    gAGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAg        <  3:462178/150‑1 (MQ=255)
                                                                                                                                     aGCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTGCCTGAAAACCCGATTATCCCTTACATTGAAGGGGAGGGAATCGGTGTAGa       >  4:325009/1‑150 (MQ=255)
                                                                                                                                      gCAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGAt      <  4:676925/150‑1 (MQ=255)
                                                                                                                                       cAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCaagaag                                                                                       <  4:121453/68‑1 (MQ=255)
                                                                                                                                       cAAACCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCaagaag                                                                                       >  3:121453/1‑68 (MQ=255)
                                                                                                                                         aaCCAGGAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTa   >  3:503263/1‑150 (MQ=255)
                                                                                                                                          aCCAGGAGCGCTCGAAGGAGCGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGCTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTaa  >  2:568930/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TAATAACGCGCATCTTTCATGACGGCAAACAATAGGGTAGTATTGACAAGCCAATTACAAATCATTAACAAAAAATTGCTCTAAAGCATCCGTATCGCAGGACGCAAACGCATATGCAACGTGGTGGCAGACGAGCAAACCAGTAGCGCTCGAAGGAGAGGTGAATGGAAAGTAAAGTAGTTGTTCCGGCACAAGGCAAGAAGATCACCCTGCAAAACGGCAAACTCAACGTTCCTGAAAATCCGATTATCCCTTACATTGAAGGTGATGGAATCGGTGTAGATGTAA  >  NC_000913_3_pae_pgi/1194959‑1195246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: