New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_pgi = 304606929 (0.630)6 (0.130) 6/276 5.4 16.4% intergenic (‑168/+99) ygfF/gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
?NC_000913_3_pae_pgi = 3046082 33 (0.740)intergenic (‑181/+86) ygfF/gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_pgi/3045921‑3046069
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccctcGCCCCTTTGGGGAGAGGGTTAGG  <  NC_000913_3_pae_pgi/3046082‑3046060
                                                                                                                                                                            
TAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC                         <  2:712255/149‑1
                      GATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGTGAGGGCCGGGGTGAGGGTAAATATTCGCGTTAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAGGGTTAG   <  1:357715/149‑1
                       ATTGCTAACCAACCGGCTCAATCTTGAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATAGTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAGGGTTAGG  <  2:32504/149‑1
                            TAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAGGGTT     <  1:526963/141‑1
                            TAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAGGGTT     >  2:526963/1‑141
                                                                                                                              CTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAG         <  1:469333/39‑1
                                                                                                                              CTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAG         >  2:469333/1‑39
                                                                                                                                                                            
TAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_pgi/3045921‑3046069
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccctcGCCCCTTTGGGGAGAGGGTTAGG  <  NC_000913_3_pae_pgi/3046082‑3046060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.