New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_pgi = 304606929 (0.630)6 (0.130) 6/276 5.4 14.8% intergenic (‑168/+99) ygfF/gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
?NC_000913_3_pae_pgi = 3504887 41 (0.920)intergenic (+104/+48) frlR/yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_pgi/3045921‑3046069
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccctcGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACGTACGGGATGTAAAATTCAACGCGCCAGCATCGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGTTAATGTCTTTTATTATCAATAGTTAAGGTGATGCGATTGGCGC  <  NC_000913_3_pae_pgi/3504887‑3504740
                                                                                                                                                                                                                                                                                                         
TAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC                                                                                                                                                      <  2:712255/149‑1
                      GATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGTGAGGGCCGGGGTGAGGGTAAATATTCGCGTTAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAGGGTTAG                                                                                                                                <  1:357715/149‑1
                       ATTGCTAACCAACCGGCTCAATCTTGAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATAGTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAGGGTTAGG                                                                                                                               <  2:32504/149‑1
                            TAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAGGGTT                                                                                                                                  <  1:526963/141‑1
                            TAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAGGGTT                                                                                                                                  >  2:526963/1‑141
                                                                                                                              CTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAG                                                                                                                                      <  1:469333/39‑1
                                                                                                                              CTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAG                                                                                                                                      >  2:469333/1‑39
                                                                                                                                                   TCGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACGTACGGGATGTAAAATTCAACGCGCCAGCATCGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGTTAATGTCTTTTATTATCAATAGTTAAGGTGATGCGATTGGCG   >  2:273011/1‑149
                                                                                                                                                    CGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACGTACGGGATGTAAAATTCAACGCGCCAGCATCGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGTTAATGTCTTTTATTATCAATAGTTAAGGTGATGCGATTGGCGC  <  1:140890/149‑1
                                                                                                                                                                                                                                                                                                         
TAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_pgi/3045921‑3046069
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ccctcGCCCCTTTGGGGAGAGGGTTAGGGTGAGGGGAACGTACGGGATGTAAAATTCAACGCGCCAGCATCGCCGGATGCGATGCTGGCGCATCTTATCCGGCCTACGGTTAATGTCTTTTATTATCAATAGTTAAGGTGATGCGATTGGCGC  <  NC_000913_3_pae_pgi/3504887‑3504740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.