Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi526,7920AC33.0% 116.3 / 98.0 113S1178R (AGC→CGC) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (34/41);  new base C (11/26);  total (45/68)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-02
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 119 total aligned reads displayed.

GGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGG  >  NC_000913_3_pae_pgi/526646‑526937
                                                                                                                                                  |                                                                                                                                                 
ggCTTGCGCAGGGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACAGCGGCGCGAAAAGCTCATCTTTATCACTGCGACCCCCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGgcgc                                                                                                                                                 <  2:222555/149‑1 (MQ=255)
 gCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCCATACGGCGTGGcgcg                                                                                                                                                >  1:518347/1‑149 (MQ=255)
  cTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACATACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGcgcgg                                                                                                                                               <  2:1142577/149‑2 (MQ=255)
   ttGCGCAGTGCGGGCTGGCGGGGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGcgcggg                                                                                                                                              <  1:668539/149‑3 (MQ=255)
   ttGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGcgcggg                                                                                                                                              <  2:952779/149‑3 (MQ=255)
   ttGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGcgcg                                                                                                                                              >  1:56147/1‑149 (MQ=255)
   ttGCGCAGGGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGcgcg                                                                                                                                              <  2:679455/149‑1 (MQ=255)
   tggcgcAGGGCGGGCAGNTGGNGNANCAACTGGCCANACTGGTGGAGACGGAATACACACCGGCGCGAAAAGCTCATCTCTATCACTGCGACCACCGGGGACTGCTGCTGGCGCTTATCAGCGTAGACGGCAATACGGCGTGGcgcggg                                                                                                                                              <  1:999216/147‑3 (MQ=11)
     gcgcGGGGCGGGCTGAGGGTGGAGCAACGGGCCAGACAGGTGGAGCCGGAATACACTCCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCGTATCCCCCAAGACGGCAATACGGCGTGGCGCGGGGa                                                                                                                                            <  2:1000745/149‑1 (MQ=11)
     gcgcAGTGCGGGCTGACGGTGGGGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACGGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGa                                                                                                                                            <  2:514394/149‑1 (MQ=255)
     gcgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGa                                                                                                                                            <  2:667289/149‑1 (MQ=255)
      cgcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGag                                                                                                                                           >  1:391253/1‑148 (MQ=255)
       gcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGagt                                                                                                                                          <  1:287792/149‑3 (MQ=255)
       gcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGagt                                                                                                                                          <  1:287908/149‑3 (MQ=255)
         agggcgggcggACGGGGGAGCAACTGGCAAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAgtat                                                                                                                                        <  1:290091/146‑1 (MQ=11)
         aGTGCGGGCTGACGGGGGAGCAACTGGCCAGACAGGTGGAGCTGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAgtat                                                                                                                                        <  1:1021659/149‑1 (MQ=11)
           ggCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatga                                                                                                                                      <  2:881708/148‑1 (MQ=255)
                  tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAATACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                               <  1:1060560/149‑1 (MQ=11)
                   ggcGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCCATACGGTGTGGAGCGCGGAATATGATGAATggg                                                                                                                              <  1:262545/147‑1 (MQ=255)
                   gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                              <  1:780378/149‑1 (MQ=255)
                   gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                                              <  1:702789/149‑1 (MQ=255)
                       gTGGAGCAACTGGCCAGCCAGGGGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGAAATCCGTCCTTGCGCGGGGAGTATGATGAATGGGGCaa                                                                                                                          <  1:318157/149‑1 (MQ=11)
                        tGGAGCAACGGGCCAGAGAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                                         <  1:1270561/149‑1 (MQ=11)
                         ggAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGCAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAcc                                                                                                                        >  2:1260986/1‑149 (MQ=255)
                          gAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCa                                                                                                                       >  1:1086376/1‑149 (MQ=255)
                           aGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAg                                                                                                                      <  2:1006750/149‑1 (MQ=255)
                            gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                     <  1:663302/149‑1 (MQ=255)
                            gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGCAGCGGGGAGTTTGGTCAATGGGGCGATCAGc                                                                                                                     >  2:255589/1‑149 (MQ=14)
                               aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTa                                                                                                                  <  2:1079981/149‑1 (MQ=255)
                               aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTa                                                                                                                  >  1:690158/1‑149 (MQ=255)
                                cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTCTCCGCGGATACTGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTaa                                                                                                                 >  1:637340/1‑149 (MQ=11)
                                cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTaa                                                                                                                 <  1:253630/149‑1 (MQ=255)
                                cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTaa                                                                                                                 <  2:1128208/149‑1 (MQ=255)
                                 tGGCCAGAGAGGTGGAGCCGGAATACACGCCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAAt                                                                                                                <  2:1087106/149‑1 (MQ=255)
                                 tGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAAt                                                                                                                <  2:576317/149‑1 (MQ=255)
                                 gggCCAGACTGGTGGAGCCGGAATACCCGCCGGCGGGAATAGCTCATCTTAATCGCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                                <  2:813892/148‑1 (MQ=11)
                                   gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCCGGGCGCCGGTAGGAAGATGAATGGGGCAACCAGCTTAAtcg                                                                                                              >  2:631899/1‑147 (MQ=11)
                                    ccgggaaGGTGGAGGAGGAATACACACCGGCGCGAAATGCTCATATTTAACACGGCGACCACCGGGGACTGCCGCTGGAGATTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgag                                                                                                             <  2:443737/143‑1 (MQ=14)
                                    ccAGGCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgag                                                                                                             <  2:950137/149‑1 (MQ=255)
                                     cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                            <  1:371426/149‑1 (MQ=255)
                                       gACAGGGGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgaggag                                                                                                          <  2:1274413/149‑1 (MQ=11)
                                          aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                       <  1:1042754/149‑1 (MQ=255)
                                          aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                       <  1:906511/149‑1 (MQ=255)
                                           ggTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATga                                                                                                              >  1:453248/1‑141 (MQ=255)
                                           ggTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAAcc                                                                                                      <  2:905973/149‑1 (MQ=255)
                                             tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCACTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGGGGAGAACCCg                                                                                                    >  1:950782/1‑149 (MQ=11)
                                               gAGCCGGAATACACACCGGCGCGAAGAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGCAGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCTACCAGCTTAATGAGGAGATCCCGca                                                                                                  >  1:1184548/1‑149 (MQ=17)
                                               gAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGca                                                                                                  >  1:857934/1‑149 (MQ=255)
                                               gAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCGGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGCATATGATGAATGGGGCAACCAGCGTAATGAGGAGAACCCGca                                                                                                  >  1:704743/1‑149 (MQ=255)
                                                aGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTAGCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcat                                                                                                 <  2:1189006/149‑1 (MQ=255)
                                                 gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGaatc                                                                                                >  1:813724/1‑149 (MQ=255)
                                                 gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCAGGGACTGCCGCTGGCGCTGATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGACTACGAGGGATGGGGCAACCAGCTTAATGAGGCGAAGCCGcatc                                                                                                >  2:1076985/1‑149 (MQ=14)
                                                   cGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcac                                                                                              <  2:787621/149‑2 (MQ=255)
                                                   cGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcat                                                                                              >  1:831595/1‑149 (MQ=255)
                                                     gAATACACACCGTCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                                     <  2:591203/140‑1 (MQ=255)
                                                     gAATACACACCGTCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                                     >  1:591203/1‑140 (MQ=255)
                                                     gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                            <  2:113820/149‑1 (MQ=255)
                                                      aaTACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgtg                                                                                           <  2:923594/149‑1 (MQ=255)
                                                           acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                     <  1:1305288/118‑1 (MQ=11)
                                                           acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                     >  2:1305288/1‑118 (MQ=11)
                                                           acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCa                                                                                      >  1:578797/1‑149 (MQ=255)
                                                           acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCa                                                                                      >  1:403502/1‑149 (MQ=255)
                                                              ccGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGCGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAGGTGTATCAGcc                                                                                   >  2:841949/1‑149 (MQ=255)
                                                                   gcgAAAGGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                              <  2:1223710/149‑1 (MQ=255)
                                                                      aacatcacATCGTTATCACTGCGATCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGTCAACTAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTc                                                                           <  1:784616/142‑1 (MQ=18)
                                                                      aaaaGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGAAGGCGCTTATCAGAGAAGACGGCAATACGGCGGGGAGCGCGAACAATGATGAATGAGGGAACCAGCTTAATGAGGAGAACACGAGTCATGTGTGTCAGCCGTACTGTc                                                                           >  2:497985/1‑149 (MQ=17)
                                                                           cTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCa                                                                      >  1:927113/1‑149 (MQ=255)
                                                                           cTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCa                                                                      <  1:40121/149‑1 (MQ=255)
                                                                           cTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCa                                                                      <  1:846837/149‑1 (MQ=255)
                                                                            tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                     >  1:509616/1‑149 (MQ=255)
                                                                             cATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCCTGTGTATCAGCCGTACCGTCGGCCAgg                                                                    >  2:639518/1‑149 (MQ=255)
                                                                             cATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAgg                                                                    >  1:520240/1‑149 (MQ=255)
                                                                               tCTTTATCACTGCGACCACCGGGGACGGCCGCTGGCGCTTATCATCGAAGACGGCAGTACAGCCAGGAGCACGGAATATGGTGCATAGAGCATCCAGCGTAGTGAGGTCAACCGGCCTCATGTGTATCAGCCGTACGGTCTTCCATGgc                                                                  >  2:696241/1‑149 (MQ=255)
                                                                                 tttATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGCGGAGCACCCGCATCATGTGTATCAGCCGTACCGTCTGCCCGGgccc                                                                >  2:262973/1‑147 (MQ=255)
                                                                                     tctcTGCGACCACCGGGGCGCGACGTCCGCACTAATCAGCGAATACGGCAATACGGCGTGGAGCGCGGAATATGATTAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagcagc                                                            <  1:507903/146‑1 (MQ=14)
                                                                                       agtGCCACCACCGGAGACGGGCGCTGGCGCTTATCAGCGAAGACGGCCAGACGGCGTGGAGGGCGGAAGATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCat                                                          <  2:365550/147‑1 (MQ=17)
                                                                                        cTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatg                                                         >  1:1216115/1‑149 (MQ=255)
                                                                                         tGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGc                                                                                                                            >  2:598282/1‑81 (MQ=255)
                                                                                          gCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                                                       <  1:1082228/149‑1 (MQ=255)
                                                                                          gCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                                                       <  2:520240/149‑1 (MQ=255)
                                                                                           cGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgatg                                                      <  1:462810/149‑1 (MQ=255)
                                                                                              ccaccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAgg                                                   <  2:56147/149‑1 (MQ=255)
                                                                                                  cGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATc                                               >  1:1012026/1‑149 (MQ=255)
                                                                                                    gggACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGTTGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAg                                             <  1:848172/149‑1 (MQ=255)
                                                                                                      gccTGCCGGTGGCGCTTATCAGCGAAGACGGCAATACCGCGTGGAGCGCGTAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAggg                                           <  1:599487/147‑1 (MQ=255)
                                                                                                           ccGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                      >  1:134983/1‑149 (MQ=255)
                                                                                                           ccGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                      <  1:692246/149‑1 (MQ=255)
                                                                                                                  cgcgaaTCAGCGAAGGCGGCAATACGGGGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAAGGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGGCTGCCAGGGCAGCAGCATGATGAGGAACCAGGGCTGTACTATAAc                               <  2:815601/144‑1 (MQ=17)
                                                                                                                          aGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCg                       >  1:332057/1‑149 (MQ=255)
                                                                                                                           gCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCt                                                                          <  2:464126/97‑1 (MQ=255)
                                                                                                                           gCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCt                                                                          >  1:464126/1‑97 (MQ=255)
                                                                                                                           gCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCgg                      <  2:567421/149‑1 (MQ=255)
                                                                                                                            cGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGt                     >  2:175812/1‑149 (MQ=255)
                                                                                                                            cGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGAGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGt                     >  1:669129/1‑149 (MQ=255)
                                                                                                                                gACGGTAATACGGCGGGGGGCGCGGAAGATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGtacta                 <  2:55505/149‑1 (MQ=255)
                                                                                                                                    gCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGAt             >  1:874790/1‑149 (MQ=255)
                                                                                                                                     gaaTACGGCGTGGAGCGCCGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATc            <  2:448235/148‑1 (MQ=255)
                                                                                                                                          cGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTg       <  2:832625/149‑1 (MQ=255)
                                                                                                                                          cGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTg       <  1:807612/149‑1 (MQ=255)
                                                                                                                                               tGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAggg  <  2:690158/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGCTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGG  >  NC_000913_3_pae_pgi/526646‑526937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑