Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_pgi 2,028,158 G→C 12.0% intergenic (‑141/+30) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi2,028,1580GC12.0% 124.0 / 10.5 52intergenic (‑141/+30)yedQ/yodCputative membrane‑anchored diguanylate cyclase/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (23/21);  new base C (2/4);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.38e-01

ACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTG  >  NC_000913_3_pae_pgi/2028016‑2028299
                                                                                                                                              |                                                                                                                                             
aCCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATcc                                                                                                                                         >  1:653313/1‑149 (MQ=255)
 cccTGACGACTCCCAACCTGCTTTTTTTTTTTTTTGTCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGCCACTACCACACCATGCCGGATGTCATcag                                                                                                                                        >  2:1000934/1‑147 (MQ=255)
  ccTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAATCTGAGATAAATTTATAATAGTGGCATGATAAAACGCGACTATACATCATTGTATGCATTAGCACACCAGGCCGGATGTGGTCAgg                                                                                                                                       >  1:391900/1‑149 (MQ=255)
   cTGACGACTCGCAACCTGCTTTTTTATGATTCTGGAATAACCAGGCGATAGCGTAGCGCAAAACGACGATATTTTTATAATGGTGGCATGGTAATACGAGACTATAACAACGTGCAGGCACTAGCACACCAGGCAGGCTGTGATccctg                                                                                                                                      >  2:100123/1‑146 (MQ=255)
    tGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGcc                                                                                                                                     >  1:436233/1‑149 (MQ=255)
          cTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCTGgtgt                                                                                                                               <  2:436233/149‑1 (MQ=255)
           tCCCAACCTTCTTTTTTAGTATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGGGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTGgtgtg                                                                                                                              <  2:552019/149‑2 (MQ=255)
           tCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGAt                                                                                                                                 <  2:637142/146‑1 (MQ=255)
           tCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGAt                                                                                                                                 >  1:637142/1‑146 (MQ=255)
             ccAACCTGCTTTTTTATTATTCTTGCATAACCTGGCGATAGTGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATCGAAGTCATGAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTaa                                                                                                                            <  1:239397/149‑1 (MQ=255)
             ccAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTaa                                                                                                                            <  1:238703/149‑1 (MQ=255)
              cAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAAt                                                                                                                           >  1:313626/1‑149 (MQ=255)
               aaCCTGCTTTTTTATGATTCTGGCATAACCTGGCGATATCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACTCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTACTg                                                                                                                          >  1:211590/1‑149 (MQ=255)
                      ttttttATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGAATGTGATCCGGCCTGATGTTAATGATACCCt                                                                                                                   >  1:519809/1‑149 (MQ=255)
                          ttATGATTCTGGCATAACCTGGCGATAGCGTACCGCCAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTca                                                                                                               >  1:993066/1‑149 (MQ=255)
                          ttATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGATTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTca                                                                                                               <  2:474379/149‑1 (MQ=255)
                          ttATGATTCTGGAATAACCTGGCGATAGCTTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTATCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTca                                                                                                               <  2:317900/149‑1 (MQ=255)
                               aTTGTGGCATAACCTGGCGAGAGAGTACCGCAAAACAGCGATAATTGTATAATGGGGGCATGATAAGACGCGACTATTATTCTTTGCAGGCATTAGCACACCAGGCCGGCTATGGTACAACTTGATGTTAATGATACCCTTTCACActt                                                                                                          <  2:468795/149‑1 (MQ=255)
                                   tGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACttcttc                                                                                                      <  2:653313/149‑1 (MQ=255)
                                      cATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGc                                                                                                   >  2:538589/1‑149 (MQ=255)
                                             tGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCCGAACGa                                                                                            >  1:668700/1‑149 (MQ=255)
                                             tGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGa                                                                                            >  1:667687/1‑149 (MQ=255)
                                              ggCTATAGCTTACCGCAAAACATTGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGAc                                                                                           <  1:154768/149‑1 (MQ=255)
                                               gCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGAGTTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGAct                                                                                          >  1:834969/1‑149 (MQ=255)
                                                   tAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCAAGATAAGACGCGACTATCATTCATTGCAGGCATTGGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGCTACCCTTTCACACTTCTTCCGCAGAACGAatcccc                                                                                      >  2:80124/1‑149 (MQ=255)
                                                          ccGCAAAACAGCGATAATTTGATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGTATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGTAGAACGACTCCCCTCCCCCg                                                                               <  2:170372/149‑1 (MQ=255)
                                                                   aGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCAGGCCTGATGTTAATGATACCATTGCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGCAACAAGcg                                                                      >  2:958842/1‑148 (MQ=255)
                                                                    gCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTggt                                                                                                                                 >  2:317588/1‑87 (MQ=38)
                                                                    gCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTggt                                                                                                                                 <  1:317588/89‑3 (MQ=38)
                                                                     cGATAGTTTTATAACGGGGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCg                                                                    <  2:659634/149‑1 (MQ=255)
                                                                     cGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcc                                                                                        >  2:727839/1‑129 (MQ=255)
                                                                     cGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcc                                                                                        <  1:727839/129‑1 (MQ=255)
                                                                           ttttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                                                              >  2:77156/1‑149 (MQ=255)
                                                                            tttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGTCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt                                                             <  1:816509/149‑1 (MQ=255)
                                                                            tttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt                                                             >  1:49187/1‑149 (MQ=255)
                                                                                      tggCATCATAAGACGCGACTATCATTCACTGCAGTCATTAGCACACCAGGCCGGATGTTATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCTCGTCACACTCTCGGTTTCATGAAAAGCTTc                                                   <  2:91481/149‑1 (MQ=255)
                                                                                           tGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGa                                                                                            >  2:85611/1‑103 (MQ=255)
                                                                                           tGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGa                                                                                            <  1:85611/103‑1 (MQ=255)
                                                                                                aGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGAcc                                         <  2:313626/149‑1 (MQ=255)
                                                                                                          aTCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGt                               <  1:77156/149‑1 (MQ=255)
                                                                                                              ttcattGCAGCCATTAGAACACCACGCCGGAGGTGATACGGCCTGATGTTAATGACACCCTTTCACACTTCTTCTGCAGAACGACTCCCCTCCCCTGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCGCGTAACCGTCATa                           <  2:950840/149‑1 (MQ=255)
                                                                                                                       cgCATTAGCATCCCAGTCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGaca                  <  2:754049/148‑1 (MQ=255)
                                                                                                                        gCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGacac                 <  2:49187/149‑1 (MQ=255)
                                                                                                                            tAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACCCCCCGCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCaa             <  1:390602/149‑1 (MQ=255)
                                                                                                                               cacaCCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCa          <  2:992415/149‑1 (MQ=255)
                                                                                                                                 cacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATa        >  2:312079/1‑149 (MQ=255)
                                                                                                                                  acCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATAc       >  2:445254/1‑149 (MQ=255)
                                                                                                                                  acCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATAc       >  1:515325/1‑149 (MQ=255)
                                                                                                                                    cAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACGCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAACTTCCCGCTTGACCCCGTAACCGACATACCAACGACACTCAACAATACCg     >  1:446238/1‑149 (MQ=255)
                                                                                                                                       ggcGGATGATATCCGGCCTGATGTAAATGATATCCTTTCTCACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCACGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGATg  <  2:13572/147‑1 (MQ=255)
                                                                                                                                       gCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTg  <  1:445254/149‑1 (MQ=255)
                                                                                                                                       gCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCCCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCATCATACCAACGACACTCAACCATACCACTg  >  1:56301/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                             
ACCCTGACGACTCCCAACCTGCTTTTTTATGATTCTGGCATAACCTGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGACCCCGTAACCGTCATACCAACGACACTCAACCATACCGCTG  >  NC_000913_3_pae_pgi/2028016‑2028299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑