Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_pgi 383,153 C→T 10.3% L261L (CTG→CTA)  yaiP ← putative family 2 glycosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi383,1530CT10.3% 105.3 / 2.6 45L261L (CTG→CTA) yaiPputative family 2 glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (20/19);  new base T (2/3);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CCAACACCACTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGA  >  NC_000913_3_pae_pgi/383014‑383297
                                                                                                                                           |                                                                                                                                                
ccAACACCACTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATa                                                                                                                                         >  1:519606/1‑149 (MQ=255)
     accacTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGAc                                                                                                                                    >  1:403316/1‑149 (MQ=255)
       cacTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACcg                                                                                                                                  >  1:796869/1‑149 (MQ=255)
        gcTCCATGGGGTCCGGTGGTGATGAATTCATGAAACCAGTTAGTGAGATAAAGCCCAACGCCATAAAGCACAACCAACAACATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACcgc                                                                                                                                 <  2:152668/148‑1 (MQ=255)
        acTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAATTCCCATCGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACcgc                                                                                                                                 <  1:291780/149‑1 (MQ=255)
                        gggggatgaATTTATAAAAGCATGTAGCATGATAAAGCACAACGCCATAAAGCACAGCCAAAAGCATAGGAACTATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGTTGTTTCTGACAGGCCGCGTATCCCACAATCCaa                                                                                                                 <  2:565087/145‑1 (MQ=255)
                         tgtgATTAATTCATTAAAACTGGTAGTGTGATAAATCCCAACGCCATAAAGCATAACCAACAACATAGGAAATATACTGAAGAAACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAAc                                                                                                                <  1:336118/148‑1 (MQ=255)
                          gtgATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCAAAATAAAAATCATTTATGCAGGAGCATACAGACCGCGTATCCCACAATCCAAcg                                                                                                               >  1:359661/1‑149 (MQ=255)
                             atgaATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAAcgccg                                                                                                            >  2:601675/1‑149 (MQ=255)
                                       tAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGc                                                                                                  <  1:923125/149‑1 (MQ=255)
                                           ccAGGTAGTGAGATAAATCACAACGCCATAAAGCCCAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGGGCATACAGACCGCGTACCCCACAATCCCACGGCGGCCGccactccact                                                                                              >  2:708266/1‑149 (MQ=255)
                                              ggTAGTGAGATACATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCActcct                                                                                           <  1:110130/149‑1 (MQ=255)
                                                 aGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGAAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCAc                                                                                        <  1:789553/149‑1 (MQ=255)
                                                  gTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGAGGATCAATAACAGTCTTTTTTTCAGGCGCATACAGCCCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCAcc                                                                                       >  2:362771/1‑148 (MQ=255)
                                                      cagaaaCCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAATTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCCCGCggg                                                                                   <  2:320713/146‑1 (MQ=255)
                                                           aTCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGtt                                                                              >  2:75206/1‑149 (MQ=255)
                                                            tCCGAACGCCATAAAGGACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTg                                                                             <  2:681830/149‑1 (MQ=255)
                                                               cAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACCCCTCACGCGGGCTGTTGCAt                                                                          >  2:840182/1‑149 (MQ=255)
                                                                 acaccATAAAGCACAACAAACAGCATAGGAAATGTACTGAAGATACCGAAGCGGCTAAAAAAAAGACTTTTACTCTGGCGCATACTGTCCGCGTATCCCACCATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTc                                                                        <  2:362629/146‑1 (MQ=255)
                                                                 aCGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTc                                                                        <  1:312381/149‑1 (MQ=255)
                                                                      aTAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCtgtg                                                                   >  1:152620/1‑149 (MQ=255)
                                                                       ttaaGCACAACCAACAGCATAGGAAATATACTGAAGATACGGAAGCGGTTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCGCACAATCCAACGCCGCCAGAGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTgg                                                                  <  2:289526/147‑1 (MQ=255)
                                                                       gaaaGCACACCCAACAGTAGAGGAAGTATGAGCAAGATACCGAAGCGGCTAAAGAAAAGTCTGTTTTGCAGGTGCATACAGACCGCGTATTCCACAATCTAACGCCGCCAGCGACGCCACTCCTCACGCGTGCTGTTGCATTCCTGTgg                                                                  <  2:438564/148‑1 (MQ=255)
                                                                           gCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGtat                                                              >  1:214506/1‑149 (MQ=255)
                                                                            cacaACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGtata                                                             <  1:601675/149‑1 (MQ=255)
                                                                               aaccaacAGCATAGGAAATAGACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGa                                                          <  1:918695/149‑1 (MQ=255)
                                                                                accaacAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGTAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGAt                                                         >  2:495896/1‑149 (MQ=255)
                                                                                accaacAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGTAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGAt                                                         >  2:495589/1‑149 (MQ=255)
                                                                                   aacAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATACAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTCCATTCCTGTGGGTATACGATGcg                                                      >  1:707037/1‑148 (MQ=255)
                                                                                             gAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGcc                                            >  1:697068/1‑149 (MQ=255)
                                                                                                atatACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGc                                         >  1:768213/1‑149 (MQ=255)
                                                                                                     cTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCAATTCGCCTGCCGAAt                                    >  2:479653/1‑149 (MQ=255)
                                                                                                           aTACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGTCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTa                              <  2:748519/149‑1 (MQ=255)
                                                                                                              ccGAAGCGGCTAAATAATAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGcc                           <  1:26350/149‑1 (MQ=255)
                                                                                                                taaGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCTCGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGt                         <  1:15914/148‑1 (MQ=255)
                                                                                                                    cGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGc                     <  1:75206/149‑1 (MQ=255)
                                                                                                                           ataaaAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCAGCCAGCGACGCCACTCCTCCCGCGGGCTCTCGCGGACCAGTTGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGACACCAAt              >  1:484531/1‑149 (MQ=255)
                                                                                                                            taaaAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATCCGGTAGCCGTTTGCCACCAATg             >  1:220693/1‑149 (MQ=255)
                                                                                                                            taaaAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATg             >  1:720047/1‑149 (MQ=255)
                                                                                                                             aaaaGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGa                                                                                                     >  2:386247/1‑60 (MQ=255)
                                                                                                                             aaaaGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGa                                                                                                     <  1:386247/60‑1 (MQ=255)
                                                                                                                                 gttttttATGCCCGCGGAAACAGACCGCGGATCCCACAATCCAACGCCGCAAGCGGCGCTCCTCTTCACGCGGGCGGTGGCATTCCGGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAg        <  2:73392/146‑1 (MQ=255)
                                                                                                                                    ttttATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGCCCAGGtc     >  2:44216/1‑148 (MQ=255)
                                                                                                                                       tATGTAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTgaga  <  1:495896/149‑1 (MQ=255)
                                                                                                                                       gaTGTAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTgaga  <  1:495589/148‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                
CCAACACCACTCCATGCGGCCCGGTGGTGATGAATTCATTAAACCAGGTAGTGAGATAAATCCCAACGCCATAAAGCACAACCAACAGCATAGGAAATATACTGAAGATACCGAAGCGGCTAAATAAAAGTCTTTTATGCAGGCGCATACAGACCGCGTATCCCACAATCCAACGCCGCCAGCGACGCCACTCCTCACGCGGGCTGTTGCATTCCTGTGGGTATACGATGCAGCGATTCGCCTGCCGAATACGGTAGCCGTTTGCCACCAATGTCCAGGTGAGA  >  NC_000913_3_pae_pgi/383014‑383297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑