Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_pgi 4,561,064 G→A 9.1% T291I (ACT→ATT)  yjiJ ← DUF1228 family putative inner membrane MFS superfamily transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi4,561,0640GA 9.1% 102.9 / 3.6 44T291I (ACT→ATT) yjiJDUF1228 family putative inner membrane MFS superfamily transporter
Reads supporting (aligned to +/- strand):  ref base G (16/24);  new base A (2/2);  total (18/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.12e-01

GCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCAGAGATGTCGTGCCCATAAAGGTACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAGCAACCAGGCACAATTGATAAGCCGACTAACGTCCAGAGATGGGCGGTTAACAATGGTG  >  NC_000913_3_pae_pgi/4560921‑4561211
                                                                                                                                               |                                                                                                                                                   
gCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCAGAGATGTCGTGCCCATAAAGGTACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAagc                                                                                                                                                >  1:318842/1‑149 (MQ=255)
  tgtccaAAGGCTAAAAGGTTGAGGTTTCCCGGGACGCTCAGTTGGGGGGCGATAGTCATCACCAGAGATGTCGTGCCCATAAAGGTACCGCCAAATCCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAagcag                                                                                                                                              <  2:484765/145‑1 (MQ=255)
   tCACAAAGCCTAAAAGATTGAGATTTCCCGGGACGCTCAGTTGGCGGGCGATAGTCATCACCAGAGATGTCGTGCCCATAAAGGTACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAcacagc                                                                                                                                             >  1:594953/1‑149 (MQ=255)
    cacaAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCAGAGATGTCGTGCCCATAAAGGTACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGACGCGAGTCGCTGGCGAGAGTAAgaagca                                                                                                                                            <  2:994950/149‑1 (MQ=255)
      caAAGCCTAAAAGCTTGAGATTTCCCGACACGCACATTTGGCGGGCGATAGTCATCACCAGAGATGTCGTGCCCCTAAAGGTACCGCCAAAACCAATACTGCTGATGCTAAGCAAGAAAGGCGACGCCCCGGCGAAAGAGAGCAGcaca                                                                                                                                          >  2:217923/1‑149 (MQ=255)
           ccTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCAGAGATGTCGTGCCCATAAAGGTACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAATAAGCAGCACACAGAt                                                                                                                                     >  1:621340/1‑149 (MQ=255)
           ccTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCAGAGATGTCGTGCCCATAAAGGTACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAATAAGCAGCACACAGAt                                                                                                                                     >  1:621817/1‑149 (MQ=255)
                     tGAGATGTCCCAGTACGCTCAGTTGGCTGGCGATAGGCATCACCAGAGATGTCGAGCCCATCAAGGTACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTCCAcc                                                                                                                           <  2:762889/149‑1 (MQ=255)
                        gaTTTCCCGGTACGCTCAGTTGGCGGGCGATAGACATCACCAGAGATGTCGTGCCCATAAAGGTACCGCGAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGGAAGCGGCACACAGATAGCCGccaccaac                                                                                                                        >  2:855715/1‑147 (MQ=255)
                          tttCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCAGAGATGTCGTGCCCATAAAGGTACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCaa                                                                                                                      >  1:21078/1‑149 (MQ=255)
                                 gTACGCTCAGTTGGCGGGCGATAGTCATCACCAGAGATGTCGTGCCCATAAAGGTACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGcc                                                                                                               <  2:681735/149‑1 (MQ=255)
                                         aGTTGGCGGGCGATAGACATCACCAGAAAAGGCGAGCCCACAAAGGGACCGCCAGAACCAAGACGGCTGATGAAAAGCAAGACAGGCACGTCGCCCACCAAAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTcaggca                                                                                                       <  2:759906/149‑1 (MQ=255)
                                                      tAGTGATCACCAGAGATGTCGTGCCCATAAAGGAACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGccccc                                                                                          <  1:850331/149‑1 (MQ=255)
                                                                cAGAGATGTCGTGCCCATAAAGGTACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTtg                                                                                <  1:31175/149‑1 (MQ=255)
                                                                        tCGTGCCCATAAAGGTACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCAt                                                                        >  1:816078/1‑149 (MQ=255)
                                                                        tCGTGCCCATAAAGGTACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCAt                                                                        >  2:973309/1‑149 (MQ=255)
                                                                         cgcgCCCATAAAGGTACCGCCAACACCAAGACCGCTGATGATAAGCAAGAGAGGCGAGTCGCTGTCGAGAGTAAGCAGCACAAAGAGAGCCTCCACCAGCAAACTCTCCGTCAGGCACGTCAAAGCATCCTCACGTTTTGCTGCCCATa                                                                       <  2:36953/146‑1 (MQ=255)
                                                                            gCCCAGAAAGGGACCGCCAAAACCGAGACTGCTGATGACAAGAAAGAGAGGCGATGCGCTCGCGAGAGTAAGCATCACACATATATCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATagcc                                                                    <  2:53455/149‑1 (MQ=255)
                                                                             cccATAAAGGTACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAATAAGCAGCACCCAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCCAAGTGATGCTGCCCATagcca                                                                   <  2:621817/149‑1 (MQ=255)
                                                                             cccATAAAGGTACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAATAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATagcca                                                                   <  2:621340/149‑1 (MQ=255)
                                                                                aTAAAGGTACCGCCAAAACCAAGACTGCTGCTGATAAGCAAGAGAGGCGAATCGCTTGCGAGAGTAAGCAGCACACAGATAGCCTGTACCAGCAAATTCACCTTGAGCAAGAGCAAAGCTCCGCAACGTTTAGCTGCGCATagccagcc                                                                >  2:201857/1‑149 (MQ=255)
                                                                                  gaaGGCACCGCCAAAACCAAGGCGGCTGATGATAACCAAGAGAGGCGAGTCGCTGGCCAGAGACAGCACCATACAGCTAGCCTGCTCCAGCAAATTCGCCGTCAAGCAGGGCAAATCCTCCCAACGTTTTGCTGCCCATAGCCAGCGaa                                                              <  2:362197/148‑1 (MQ=255)
                                                                                       tACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAGCaa                                                         >  2:655787/1‑149 (MQ=255)
                                                                                          cGCCAAAACCAAGACTGCGGATGAAAAGCAAGAGAGGCTAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAGCAACCa                                                      <  2:365315/149‑1 (MQ=255)
                                                                                          cGCAAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCCCAAGCAAATTCGACGTCAGCAAGGGCCAAGCCAACAATCGTTTTGCCGCTCATAGCCAGTCAAAGcttcca                                                      <  2:92692/149‑6 (MQ=255)
                                                                                            ccAAAAACAAGACTGCTGATGATAAGCAAGAGAGGCGAGTTGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAAATCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAGCAACCAgg                                                    <  1:208990/149‑1 (MQ=255)
                                                                                                      gACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGATTTGCTGCCCATAGCCAGCCAAAGCAACCAGGCACAATTGAt                                          >  1:956581/1‑149 (MQ=255)
                                                                                                        ctgctgATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCATGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAGCAACCAGGCACAATTGATaa                                        <  2:273413/149‑1 (MQ=255)
                                                                                                         tgttgaggaTAGGCAAGACGGGGGAGTCGCGGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCACCCAATGTTTTGCTGCCCATAGCCAGCCAAAGCAACCAGGCACAATTGATAAg                                       <  2:72936/146‑1 (MQ=255)
                                                                                                          gtcgatgatATGCAAGAGAGGAGAGTCGCCGGCTATAGTAAGCAGCAAACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCCACGTTCTGCTGCCCATAGCCAGTCAACGCAACCAGGCACAATTGCTAAGc                                      <  2:866860/146‑1 (MQ=255)
                                                                                                           ctgATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAGCAACCAGGCACAATTGATAAGcc                                     <  2:318842/149‑1 (MQ=255)
                                                                                                            tgatgaTAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAg                                                            >  1:673906/1‑125 (MQ=255)
                                                                                                            tgatgaTAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAg                                                            <  2:673906/125‑1 (MQ=255)
                                                                                                                    aGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAGCAACCAGGCACAATTGATAAGCCGACTAACGt                            >  1:163984/1‑149 (MQ=255)
                                                                                                                     gCAAGCGAGCGGGGTCGGTCGCCAGAGTAATCAGCACACAGCTAGCCTGCACCAGCAAATTCGCCGTCAGCCAGGGTAAAGCCCCCCAACGTTTTGCGGCCCCTAGCCAGCTACAGCAACCAGGCACAATTGATAAGCCGACTAACGTc                           <  2:85364/149‑1 (MQ=255)
                                                                                                                      cAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCACCCCATAGCCAGCCAAAGCAACCAGGCACAATTGATAAGCCGACTAACGTcc                          >  1:784573/1‑149 (MQ=255)
                                                                                                                       aaGAGAGCCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTTCTGCCCATAGCCAGCCAAAGCAACCAGGCACAATTGATAAGCCGACTAACGTCCa                         <  1:193565/149‑1 (MQ=255)
                                                                                                                        tggggtgCGGGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAGCAACCAGGCACAATTGATAAGCCGACTAACGTCCag                        <  2:753242/143‑1 (MQ=255)
                                                                                                                              gCGAGTCGCTGGCGTGAGTAGGTAGCACACAGATAGCCTGCACCAGCAAGTTCGCCGTCAGGCAGGGCAAAGCCCCCGAACGTTTTGCTGCCCATAGCCAGCCAAAGCAATCTGGTACAATTGATAAGCCGACTAACGTCCAGAGATgg                  <  2:61756/149‑1 (MQ=255)
                                                                                                                              gCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTGGCCGTCGGACAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAGCAACCAGGAACAATTGAAAAACCCACTAACGTCCAGAGATgg                  >  2:919213/1‑149 (MQ=255)
                                                                                                                                      cTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAg                                                            >  1:341281/1‑99 (MQ=255)
                                                                                                                                      cTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAg                                                            <  2:341281/99‑1 (MQ=255)
                                                                                                                                         gCGGGAGTAAGCCGCCTACAGATAGCCGGCACTAGCAAATTCGCCGTCAGGCAGTGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAGCAATCAGGCACAGGTGATTAGCCCATTAAGGTTCGGAGATGGGCGGTTAACaa       <  2:261573/149‑1 (MQ=255)
                                                                                                                                           gagaGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCTAAAGCAACCAGGCACAATTGATAAGCCGACTAACGTCCAGAGATGGGCGGTTTACAATg     >  2:929710/1‑149 (MQ=255)
                                                                                                                                             gagTAAGCAGCACACAGATAGCCTGTACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGTCAGCCAAAGCAACCAGGCACAATTGATAAGCCGACTAACGTCCAGAGATGGGCGGTTAACAATGGt   <  2:896605/149‑1 (MQ=255)
                                                                                                                                             gagTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCGGGGCAAAGCCCCCCCACGTTTTGCTGCCCATAGCCAGCCAAAGCAACCAGGCACAATTGATAAGCCGACTAACGTCCAGAGATGGGCGGTTAACAATGGt   >  1:884163/1‑149 (MQ=255)
                                                                                                                                              agTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAGCAACCAGGCACAATTGATAAGCCGACTAACGTCCAGAGATGGGCGGTTAACAATGGTg  >  1:703874/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GCGTCACAAAGCCTAAAAGATTGAGATTTCCCGGTACGCTCAGTTGGCGGGCGATAGTCATCACCAGAGATGTCGTGCCCATAAAGGTACCGCCAAAACCAAGACTGCTGATGATAAGCAAGAGAGGCGAGTCGCTGGCGAGAGTAAGCAGCACACAGATAGCCTGCACCAGCAAATTCGCCGTCAGGCAGGGCAAAGCCCCCCAACGTTTTGCTGCCCATAGCCAGCCAAAGCAACCAGGCACAATTGATAAGCCGACTAACGTCCAGAGATGGGCGGTTAACAATGGTG  >  NC_000913_3_pae_pgi/4560921‑4561211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑