Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_pgi 526,796 C→G 19.9% A1179G (GCG→GGG)  rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi526,7960CG19.9% 144.3 / 29.6 71A1179G (GCG→GGG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (21/34);  new base G (9/5);  total (30/41)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTG  >  NC_000913_3_pae_pgi/526649‑526932
                                                                                                                                                   |                                                                                                                                        
ttGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGcgcg                                                                                                                                         <  1:555712/149‑1 (MQ=255)
ttGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCGCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCGGCGAAGACGGTAAAACGGCGTGGAGcgcg                                                                                                                                         >  1:898907/1‑149 (MQ=255)
   cgtagggCGGGCTGACGGTGGAGCAACTGGCCAGAAAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGaa                                                                                                                                      <  2:194195/143‑1 (MQ=255)
   cgcAGTGCGGGCGGCCGGGGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGaa                                                                                                                                      <  2:991561/149‑1 (MQ=255)
    gcAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATTTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGACGTGGCGCggggggt                                                                                                                                     >  1:12098/1‑146 (MQ=11)
      aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                                   <  1:320322/149‑1 (MQ=255)
        tGCGGGCTGACGGTGGGGCAACTGGGCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCAACGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatga                                                                                                                                 <  1:424998/149‑1 (MQ=255)
        tGCGGGCTGACGGTGGAGCAACTGGCCATACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatga                                                                                                                                 <  1:41615/149‑1 (MQ=255)
         gggggtggACGTTGTAGCATCTGCCCAGTACGGTGGAGCCGGAATACACACCGTCGCGAAAAGCTTATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCGGCGGAGACGGCAATACGGCGTGGAGCGCGGAATatgat                                                                                                                                <  2:423697/142‑1 (MQ=17)
               tggcGGTGGAGCAACTGACCAGACAGGGTGAGCAGGAATACACCTCGGCGCGAAAATCTCATCTTTACCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgg                                                                                                                          <  2:22482/146‑1 (MQ=14)
               tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATAAGCGAAACAGGAAAAAAGTGGGGGAGAGCGGAATATGAGGaagtt                                                                                                                          >  2:655706/1‑146 (MQ=14)
               tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAACTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGAGCGGCGCGGGGAGTAGGATGAAtag                                                                                                                          >  1:889004/1‑147 (MQ=11)
                gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCCCTGAGACCACCGGGGACTGCCGCTGGCGCGTATCAGCGCAGACGGCAAGACGGACAGGAGCGCGCAAAACAATGaaggca                                                                                                                         >  2:799475/1‑145 (MQ=14)
                gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                                         <  1:834313/149‑1 (MQ=255)
                   ggTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTGATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCa                                                                                                                      >  1:788596/1‑149 (MQ=255)
                     tGGGGCATCTGGCCAGACAGGTGGTGCCGGTATACTCTCTGGCGGGAAGAGTTCATCTTTATCACTGCGACCACGGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAc                                                                                                                    <  1:473035/149‑1 (MQ=14)
                     tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAc                                                                                                                    <  2:830377/149‑1 (MQ=255)
                       gAGGAACTGGCTAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCa                                                                                                                  <  1:472310/149‑1 (MQ=255)
                       gAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCa                                                                                                                  <  1:806306/149‑1 (MQ=255)
                       gAGCAACTGGCCAGACAGGTGGAGCAGGCATACACACCGCCGCGGAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCa                                                                                                                  >  1:396520/1‑149 (MQ=11)
                        aGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAg                                                                                                                 >  1:376857/1‑149 (MQ=255)
                         gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATATTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                <  1:926964/149‑1 (MQ=1)
                             cTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTaa                                                                                                            >  1:921156/1‑149 (MQ=255)
                              tGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                           >  1:855650/1‑149 (MQ=255)
                              tGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTAGCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                           <  2:565250/149‑1 (MQ=11)
                                gCCATACAGGTGGGGCCGGAATACACACCGGCACGCACAGGTCGTCCTTGTCACTGCGACCATCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATga                                                                                                         <  2:712069/149‑1 (MQ=11)
                                  cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                       >  1:742636/1‑149 (MQ=255)
                                  cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                       >  1:817637/1‑149 (MQ=255)
                                  cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATTAATGGGGCAACCAGCTTAATgagg                                                                                                       >  1:245887/1‑149 (MQ=255)
                                   aggcAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGACGAATGGGGCAACTAGCTTAATgagga                                                                                                      <  2:745007/146‑1 (MQ=255)
                                     aCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                          <  2:1001718/127‑1 (MQ=255)
                                     aCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                          >  1:1001718/1‑127 (MQ=255)
                                       aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGATTAATGAGGAGAAc                                                                                                  >  1:516475/1‑149 (MQ=255)
                                             gggcGGAATACACACCGGGGCGAGATGTTCATTTTTATCACTGCGACCACCTGGGACTGCCGCTGGGGCTTATCGGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcat                                                                                            <  2:775599/146‑1 (MQ=14)
                                               ccGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatca                                                                                          >  1:920482/1‑149 (MQ=255)
                                               ccGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGGATATGATGAATGGGGCAACCAGCTTAATGAGGAGCACCCGcataa                                                                                          >  2:71870/1‑147 (MQ=255)
                                               ccGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatca                                                                                          <  1:542447/149‑1 (MQ=255)
                                                cGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCCGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGAAACCAGCTAAATGAGGAGAACCCGcctcac                                                                                         >  2:417319/1‑148 (MQ=11)
                                                 ggAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacc                                                                                        <  1:417319/149‑3 (MQ=255)
                                                  gAATACAGACCGGCGCGAAAAGCTCATTTTTATCACTACTACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                       <  1:439567/149‑1 (MQ=255)
                                                    ctACACTCCGGCGCGAAAAGCTCAGCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGTCGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATgtgt                                                                                     <  2:997242/148‑1 (MQ=255)
                                                      acacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGAGTGCCGTTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTAt                                                                                   <  1:441794/149‑1 (MQ=255)
                                                      acacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgg                                                                                                                          >  1:578358/1‑110 (MQ=255)
                                                      acacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgg                                                                                                                          <  2:578358/110‑1 (MQ=255)
                                                       cacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATc                                                                                  <  2:902534/149‑1 (MQ=255)
                                                         ctcggacgcgAAACGCTCAACTTTCTGCCTGCGACCACCGGGGACTCCCGGTGGCGCTTATCAGGGAAGACGGCAATACGGCGTGGAGCGTGGAATATGATCAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAg                                                                                <  2:169636/143‑1 (MQ=17)
                                                                gacaaaaGCGCGTCTTTGTGATCGCGGCCACGGGGGCAGGGCGGTGGCGCTTATCAGCGAAGACGGCAATACGGCGGGGAGCGCGGAATATGATGAATGGGGCAATCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                         <  2:168199/146‑1 (MQ=17)
                                                                    aaaGCTCATCTTTATCCCGGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCt                                                                     <  1:49837/149‑1 (MQ=255)
                                                                      aGCTCAGCTGTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGc                                                                   <  2:642573/149‑1 (MQ=255)
                                                                        cTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCa                                                                 <  2:728516/149‑1 (MQ=255)
                                                                          cATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAgg                                                               >  1:984079/1‑149 (MQ=255)
                                                                             cTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACCGCCGCGAGAGCGGAGTATGATGAATGCGGAAAGCAGCTCAACGCGGAGAACCGGCATCCAGTGTGTCGGCCGTCCCGTCTGCCatgcca                                                            >  2:757069/1‑144 (MQ=12)
                                                                               ttATCACTGCGACCACCGGGGACTGCCGCAGGGGCTTATCAGCGAAGAAGGCAATACCGCGAGGGACGCGGGGTATGATCGAAAGGTCAACCAGCTAAATGCCTCGAGCCCGCGTCACGTGGTTAAGCCGTAACGACTACCAGGccaag                                                          >  2:411239/1‑147 (MQ=255)
                                                                                tATCACTGCGACCACCGGGGACTGCCGCGGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagca                                                         <  2:137477/149‑1 (MQ=255)
                                                                                         gACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAtgatga                                                <  2:788596/149‑1 (MQ=255)
                                                                                              ccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCGGCAGAAAGATGAGGAAt                                           >  1:718239/1‑149 (MQ=255)
                                                                                               cGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTAACAGCCGTACCGTCTACCAGGGCAGCAGCATGATGAGGAATc                                          >  1:16214/1‑149 (MQ=255)
                                                                                                         cGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTAc                                >  1:230648/1‑149 (MQ=255)
                                                                                                         cGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTAc                                >  1:997206/1‑149 (MQ=255)
                                                                                                            tGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACtat                             <  1:371333/149‑1 (MQ=255)
                                                                                                                gcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAATCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAcc                         <  2:558082/149‑1 (MQ=255)
                                                                                                                       aGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCg                  >  2:462180/1‑149 (MQ=255)
                                                                                                                       aGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGCCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCg                  <  1:74502/149‑1 (MQ=255)
                                                                                                                          gAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGta               >  2:988978/1‑149 (MQ=255)
                                                                                                                          gAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGCGTATCAGCCGTACCGTCTCCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGta               <  1:654983/149‑1 (MQ=255)
                                                                                                                             gACGGCGATGAGGCGTGGCGCGAGGAATATGTGGAATGGGGCACCCAGCTTAATGGGCAGAACCCGCATGATGTGGAGCAGCCTTACCGTCTGCCAGGGCCGCCGCATGATGCGGAATCAGGGCTGTACTATAACCGTCACCGGtacta            <  2:576574/149‑1 (MQ=14)
                                                                                                                                ggCAAGACGGCGTGGAGCGCGGAAGATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGa         <  2:130509/149‑1 (MQ=255)
                                                                                                                                  cAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCAGCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAAAATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGATACAACAATc       >  1:88595/1‑149 (MQ=17)
                                                                                                                                   aaTAGGGCGTGGAGCGCTGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATAATGTGTATCAGCCGGACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATcc      <  1:109222/149‑1 (MQ=255)
                                                                                                                                   aaTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGCGAACCCCAGTGATAGGTGTCAGCTGTACCGTCTGCTAGTGCATCATTAGAAGCTTGAAGCATGGCTGTACTATAACCGTCCCCGGTACTCCGGTcc      >  2:463441/1‑149 (MQ=12)
                                                                                                                                   aaTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAggg                                                              <  1:22473/93‑1 (MQ=255)
                                                                                                                                   aaTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAggg                                                              >  2:22473/1‑93 (MQ=255)
                                                                                                                                   aaTACGGCGTGGAGCGCGGAATATGATGAAGGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATAAGCCGTACCGTCTGCCAGGGCGGCAGCATGCTGGGGGATCAGGGCTGAACTCTAACCGTCGCCGGTACTACGATct      >  2:234054/1‑148 (MQ=17)
                                                                                                                                       cGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTg  <  1:999986/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                        
TTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTG  >  NC_000913_3_pae_pgi/526649‑526932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: