Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913_3_pae_pgi 621,005 G→C 9.4% R62G (CGC→GGC)  fepG ← iron‑enterobactin ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi621,0050GC 9.4% 151.8 / 7.5 53R62G (CGC→GGC) fepGiron‑enterobactin ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (29/19);  new base C (3/2);  total (32/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01

CAGCACCATCGCCACCAGCACGCCGCTCCACGCCCCGGTGTTAAAGCCCATTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAA  >  NC_000913_3_pae_pgi/620859‑621153
                                                                                                                                                  |                                                                                                                                                    
cAGCACCATCGCCAACTGCACGCCGCCCCACGCCCCGGTGTTAAAGCCCATTACGTCAGGGCTGCCGAGCGGGTTACGCATCGGCGACAGAAAAAGCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACGGCGCCATCAGCACGCGTg                                                                                                                                                    <  2:991824/149‑1 (MQ=255)
     ccATCGCCCCCAGCACGCCGCTCCACGCCCTGGTGTTGATGTCCCTTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGTAAGATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTaaa                                                                                                                                               <  1:840243/149‑1 (MQ=255)
        tCGCCACCAGCACGCCGCTCCACGCCCCGGTGTTAAAGCCCATTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGc                                                                                                                                            <  2:718544/149‑1 (MQ=255)
          gCCACCAGCACGCCGCTCCACGCCCCGGTGTTAAAGCCCATTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCa                                                                                                                                          <  1:213946/149‑1 (MQ=255)
             accaGCACGCCGCTCCACGCCCCGCTGTTAAAGCCCATTACGTCAGGGCTGCCGCGCGGGTTACGCATCAGCGACCGAAAAACCGCGCCACTGACGCCCAGTGCTGCGCCAACCCACAGCCCCATCCGCACGCCTCGCAACCGccctcc                                                                                                                                       >  2:176367/1‑145 (MQ=255)
                     gccgcTCCACGCCCCGGTGTTAAAGCCCATTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCCTGGTAAACGCCATTCGGTGacca                                                                                                                               >  1:372339/1‑149 (MQ=255)
                     gccgcTCCACGCCCCGGTGTTAAAGCCCATTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCCTGGTAAACGCCATTCGGTGacca                                                                                                                               >  1:372375/1‑149 (MQ=255)
                         cTCCACGCCCCGGTGTTAAAGCCCATTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCAt                                                                                                                           <  1:266553/149‑1 (MQ=255)
                         cTCCACGCCCCGGTATTAAAACCCATTACGTCAGGGCTGCCGAGCGGGTTACGCAACAGCGACTAAAAAATCGCGCCACTGACGCCCGGTGCTGCGCAAAGCAACAGCGCCATCAGCCAGCGTAGGAAACGCCACTCGCTGAccaccac                                                                                                                           >  2:525848/1‑148 (MQ=255)
                          tCCACGCCCCGGTGTTAAAGCCCCTTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATc                                                                                                                          <  1:481261/149‑1 (MQ=255)
                          tCCACGCCCCGGTGTTAAAGCCCATTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATc                                                                                                                          >  1:752624/1‑149 (MQ=255)
                          cccACGCCCCGGTGTTAAAGCCCATTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATc                                                                                                                          <  1:456020/148‑1 (MQ=255)
                                  ccGGTGTTAAAGCCCATTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGCCTGAAAAATCGCGCCACTGGCGCCCAGTCCTGCGCCAACCAACACCGCCATCAGCCCACGTGGCAAACGCCCTCCGGCCACCACCATCGCCCTACt                                                                                                                  >  2:430496/1‑149 (MQ=255)
                                  ccGGTGTTAAAGCCCATTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGATACGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATaat                                                                                                                  >  1:987115/1‑147 (MQ=255)
                                      tgtTAAAGCCCATTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCGCTGACGCCCAGTGCTGCGTCAACAAATAGCCGCAGCACCGCTCGCGGTAAACGCCATTCGCTGACCACCATCTTCATACTgctc                                                                                                              >  2:965339/1‑147 (MQ=255)
                                           aaGCCCATTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGgcgc                                                                                                         >  1:549008/1‑149 (MQ=255)
                                            aGCCCATTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCCCGGCGCa                                                                                                        >  1:110276/1‑149 (MQ=255)
                                              cccATTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATc                                                                                                      >  1:754543/1‑149 (MQ=255)
                                              cccATTACGTCAGGACTGCCGAGCAGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGACATACTGCGCGGCGCATc                                                                                                      >  1:868771/1‑149 (MQ=255)
                                                 aTTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGcc                                                                                                   >  2:623933/1‑149 (MQ=255)
                                                  ttACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGccc                                                                                                  >  1:878810/1‑149 (MQ=255)
                                                    aCGTCAGGGCTGCCGATCGGGTTACTCATTAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCAt                                                                                                <  1:269187/149‑1 (MQ=255)
                                                      gTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCa                                                                                              >  2:540825/1‑149 (MQ=255)
                                                           gacTGCCGTGCATGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCAATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGcgcg                                                                                         >  2:10799/3‑149 (MQ=255)
                                                                             gCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCCGCACGCGTGGTAAACACCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGGGCATCAGCGCGGCGAATACCTGCGAGGtt                                                                       >  1:293841/1‑149 (MQ=255)
                                                                                      aCTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCACATCGCCCATCAGCGCGCCCAATAGCTCCGAGGTTTCCAACGTg                                                              >  1:759713/1‑149 (MQ=255)
                                                                                          aaaaaTCGCGCCACTGACGCCCAGTGCTGCGCCAATTACCACCGCCATCAGCACGCGTGGTAAACGCCATTCGGTAACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCAAGCGTGACgg                                                          >  1:38751/1‑149 (MQ=255)
                                                                                          aaaaaTCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACgg                                                          >  2:237458/1‑149 (MQ=255)
                                                                                             aaTCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCCTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCATCGTGACGGCAc                                                       <  2:372375/149‑1 (MQ=255)
                                                                                             aaTCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCCTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCATCGTGACGGCAc                                                       <  2:372339/149‑1 (MQ=255)
                                                                                                 gcgcTACTGACGCCCAGTGATGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCTATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCt                                                   <  2:835270/149‑1 (MQ=255)
                                                                                                 gcgcCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGATAAACGCCATTCGGTGACCACCATCGACATACTGCGCGGCGCATCGCCCATCAGCGCGGCGACTACCTGCGAGGTTCCCCGCGTGACGGCCCCGCt                                                   >  2:943414/1‑149 (MQ=255)
                                                                                                   gcCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCTAGCGTGACGGCagtgctac                                                 >  1:376975/1‑142 (MQ=255)
                                                                                                   gcCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACcgccgc                                                 >  1:672119/1‑149 (MQ=255)
                                                                                                          aCGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCAACGCGCCGTAATcc                                          >  2:444904/1‑149 (MQ=255)
                                                                                                            gCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATcccc                                        <  2:798367/149‑1 (MQ=255)
                                                                                                            gCCCAGTGATGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATcccc                                        <  1:49044/149‑1 (MQ=255)
                                                                                                            gCCCAGTGATGCGACAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGAGTAATcccc                                        >  2:623316/1‑149 (MQ=255)
                                                                                                              ccAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTTACGGCACCGCTGCGTCATCCCCAg                                      >  2:552967/1‑149 (MQ=255)
                                                                                                              ccAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAg                                      <  1:623933/149‑1 (MQ=255)
                                                                                                                     tgcGCCAATCAACAGGGCCATCAGCACGCGTGGTACACGCCATTCGGTGACCGCCAGCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCCGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTg                               <  2:712262/149‑1 (MQ=255)
                                                                                                                        gcCAATCAACAGCGCCATCAGCACGCGTGGTCCACCAGGTACGTTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGGGGTTTCCAGCGTGACGGCACCGCTGCGGAAAACCCATATACCTGCaa                            >  1:491116/1‑149 (MQ=255)
                                                                                                                         ccAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCCGCGTAATCCCCAGATACCTGCAAc                           >  1:562557/1‑149 (MQ=255)
                                                                                                                          cAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGGCCACCATCGTCATACTGTGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATACCAAGCTACATgagacc                          >  1:532128/1‑144 (MQ=255)
                                                                                                                            aTCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACcac                        <  2:878810/149‑1 (MQ=255)
                                                                                                                             tCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACcaca                       >  2:837347/1‑149 (MQ=255)
                                                                                                                                  aGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCCGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGc                  <  1:237458/149‑1 (MQ=255)
                                                                                                                                  aGCGCCACCAGCCGGGGTGGTCACCGCCATTCGGTCACCCCCATCGTCAGACTTCGCGGCGCACCGCCCATCATCGCGGTGAATACCTGCGAGGTTTCCAGCGTGACGGCACCTCTGCGTAATCCCCAGATACCTGCAACCACACAGGc                  <  2:699557/149‑1 (MQ=255)
                                                                                                                                       cATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCCACCACACAGGCGGaaa             >  2:937525/1‑149 (MQ=255)
                                                                                                                                        aTCAGCACGCGTGGTAAACGCCATTCGGTTACCACCGTCGTCATACTGCGGGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTACAGCGTGACGGCACCGCTGCGTAATCCCCAGATACATGCAACCACACAGGCGGAAAc            >  2:819395/1‑149 (MQ=255)
                                                                                                                                          cAGCACGCGTAGTAAAGGCCATTCGGTGACCACCATCGCCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACca          <  2:1001622/149‑1 (MQ=255)
                                                                                                                                             cACGCGTGGTACACGCCAATCGGTGGCCTGCATCCTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACcagca       <  1:272066/149‑1 (MQ=255)
                                                                                                                                              aCGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACcagcag      <  1:965339/149‑1 (MQ=255)
                                                                                                                                                  gTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCaaa  <  1:540825/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CAGCACCATCGCCACCAGCACGCCGCTCCACGCCCCGGTGTTAAAGCCCATTACGTCAGGGCTGCCGAGCGGGTTACGCATCAGCGACTGAAAAATCGCGCCACTGACGCCCAGTGCTGCGCCAATCAACAGCGCCATCAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCGGCGCATCGCCCATCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAA  >  NC_000913_3_pae_pgi/620859‑621153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑