Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 4,158,929 C→A A147E (GCG→GAG)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi4,158,9290CA92.4% 361.9 / 22.0 119A147E (GCG→GAG) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (4/5);  new base A (52/58);  total (56/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.11e-01

Only 100 of 119 total aligned reads displayed.

CCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAG  >  NC_000913_3_pae_pgi/4158785‑4159075
                                                                                                                                                |                                                                                                                                                  
ccACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTgg                                                                                                                                               >  2:6429/1‑150 (MQ=255)
ccACAGTTGGACCCTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTgg                                                                                                                                               >  2:1033208/1‑150 (MQ=255)
 cacaGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGt                                                                                                                                              >  1:1439851/1‑150 (MQ=255)
      ttGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAag                                                                                                                                         >  2:531416/1‑150 (MQ=255)
       tGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAaga                                                                                                                                        <  3:320947/150‑1 (MQ=255)
         gACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAagaga                                                                                                                                      <  2:748520/150‑1 (MQ=255)
            cGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCg                                                                                                                                   >  2:87197/1‑150 (MQ=255)
              tACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGaa                                                                                                                                 >  1:174091/1‑150 (MQ=255)
                ccTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGc                                                                                                                               >  2:1325376/1‑150 (MQ=255)
                  tGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGcat                                                                                                                             >  2:958118/1‑150 (MQ=255)
                   gCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGcatt                                                                                                                            <  2:2058/150‑1 (MQ=255)
                     tACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGcattca                                                                                                                          <  2:143940/150‑1 (MQ=255)
                     tACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGcattca                                                                                                                          >  1:143940/1‑150 (MQ=255)
                      aCCGCATATTATCCCTATCCTGCACCAGACCTTACCAAAGCTCGCAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGACACAGAGCGAAGccttcat                                                                                                                         >  2:1280347/1‑150 (MQ=255)
                       ccGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGcattcatt                                                                                                                        <  2:1280442/150‑1 (MQ=255)
                        cGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTg                                                                                                                       >  2:1244300/1‑150 (MQ=255)
                         gCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGa                                                                                                                      >  1:897708/1‑150 (MQ=255)
                         gCATATTATCCCTATGCTGCACCAGACCTTTCCAAAACTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGa                                                                                                                      >  4:714689/1‑150 (MQ=255)
                          cATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGaa                                                                                                                     <  2:390056/150‑1 (MQ=255)
                           atatTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAg                                                                                                                    <  2:568235/150‑1 (MQ=255)
                             attatCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTg                                                                                                                  <  2:26529/150‑1 (MQ=255)
                              ttatCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGt                                                                                                                                              >  2:589991/1‑121 (MQ=255)
                                    cTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCgttgt                                                                                                           <  4:532774/150‑1 (MQ=255)
                                        gctgcACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTtga                                                                                                       <  1:6429/150‑1 (MQ=255)
                                           gcACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTtgatga                                                                                                    <  4:407452/150‑1 (MQ=255)
                                             aCCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGc                                                                                                  <  4:441985/150‑1 (MQ=255)
                                              ccAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGcc                                                                                                 >  2:182090/1‑150 (MQ=255)
                                                 gACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAt                                                                                              <  2:678309/150‑1 (MQ=255)
                                                 gACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAAt                                                                                              <  2:988080/150‑1 (MQ=255)
                                                    cTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGtt                                                                                           >  4:647683/1‑150 (MQ=255)
                                                      ttCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGttt                                                                                                         >  1:574755/1‑134 (MQ=255)
                                                      ttCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGttt                                                                                                         <  2:574755/134‑1 (MQ=255)
                                                       tCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCt                                                                                        <  1:1377970/150‑1 (MQ=255)
                                                          aaaGCTGGAAATGTAGCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGc                                                                                     <  3:279963/150‑1 (MQ=255)
                                                               tGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGctatct                                                                                <  2:897708/150‑1 (MQ=255)
                                                                   aaTGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGa                                                                            >  3:830770/1‑150 (MQ=255)
                                                                    aTGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGATCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGaa                                                                           <  1:1461042/150‑1 (MQ=255)
                                                                     tGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAg                                                                          <  1:1244300/150‑1 (MQ=255)
                                                                      gTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGa                                                                         <  2:1207939/150‑1 (MQ=255)
                                                                       tATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGAt                                                                        <  3:733080/150‑1 (MQ=255)
                                                                       tATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGAt                                                                        <  1:1033208/150‑1 (MQ=255)
                                                                       tATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATc                                                                       >  2:854429/1‑150 (MQ=255)
                                                                           tGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGTCAATGTTGCTGGCTATCTATGAAGATCAcc                                                                    >  4:684520/1‑150 (MQ=255)
                                                                           tGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCAcc                                                                    <  2:700379/150‑1 (MQ=255)
                                                                              aTGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGt                                                                 >  4:50800/1‑150 (MQ=255)
                                                                               tGAAGCACAGACCCACCAGTTACTGGCGCAACTGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTgg                                                               <  1:854429/150‑1 (MQ=255)
                                                                                gAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGct                                                                                    <  3:22061/129‑1 (MQ=255)
                                                                                gAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGct                                                                                    >  4:22061/1‑129 (MQ=255)
                                                                                 aaGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAaga                                                                                                                                        >  4:83244/1‑76 (MQ=255)
                                                                                 aaGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAaga                                                                                                                                        <  3:83244/76‑1 (MQ=255)
                                                                                 aaGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTtga                                                                                                       >  1:1014576/1‑109 (MQ=255)
                                                                                 aaGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTtga                                                                                                       <  2:1014576/109‑1 (MQ=255)
                                                                                 aaGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGcat                                                                                                                             <  1:734707/87‑1 (MQ=255)
                                                                                 aaGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGcat                                                                                                                             >  2:734707/1‑87 (MQ=255)
                                                                                 aaGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTggg                                                              <  4:830770/150‑1 (MQ=255)
                                                                                    cacaGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGa                                                           >  4:573478/1‑150 (MQ=255)
                                                                                          cccACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACcgcg                                                     <  1:87197/150‑1 (MQ=255)
                                                                                           ccaccaGTTACTGGCGCAACTGGACAGCGGCAGACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGCAGATCACCCGTGGGCGAACCGCGa                                                    >  2:147511/1‑150 (MQ=255)
                                                                                           ccaccaGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGcattcat                                                                                                                         <  1:824199/81‑1 (MQ=255)
                                                                                           ccaccaGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGa                                                    <  1:1196876/150‑1 (MQ=255)
                                                                                            caccaGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAg                                                                                                   >  4:333859/1‑102 (MQ=255)
                                                                                            caccaGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAg                                                                                                   <  3:333859/102‑1 (MQ=255)
                                                                                            caccaGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGaa                                                   <  1:199317/150‑1 (MQ=255)
                                                                                             cccaGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGTTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAAt                                                  <  3:722012/149‑1 (MQ=255)
                                                                                                aGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCg                                               <  3:647683/150‑1 (MQ=255)
                                                                                                aGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCg                                               >  1:377389/1‑150 (MQ=255)
                                                                                                 gTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGt                                              <  1:1325376/150‑1 (MQ=255)
                                                                                                   tACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTAc                                            <  3:225631/150‑1 (MQ=255)
                                                                                                   tACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTAc                                            >  1:357702/1‑150 (MQ=255)
                                                                                                      tGGCGCAACGGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGa                                         >  2:1034745/1‑150 (MQ=255)
                                                                                                       ggCGCAACTGGACAGCGGCAAACTCGAATGCGTGATCCTCGAGCTGGTGAAAGAGAGCGACGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTGTCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGAt                                        >  1:1072007/1‑150 (MQ=255)
                                                                                                        gcgcAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATg                                                                                             <  2:1060114/96‑1 (MQ=255)
                                                                                                        gcgcAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATg                                                                                             >  1:1060114/1‑96 (MQ=255)
                                                                                                        gcgcAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTtga                                                                                                       >  4:182207/1‑86 (MQ=255)
                                                                                                              cTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGttt                                                                                                         <  4:490532/78‑1 (MQ=255)
                                                                                                              cTGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGttt                                                                                                         >  3:490532/1‑78 (MQ=255)
                                                                                                               tGGACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATc                                <  1:369432/150‑1 (MQ=255)
                                                                                                                 gACAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTg                              <  1:1034745/150‑1 (MQ=255)
                                                                                                                  aCAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTgg                             >  1:596961/1‑150 (MQ=255)
                                                                                                                  aCAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTgg                             >  1:1268004/1‑150 (MQ=255)
                                                                                                                   cAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGaa                                                                           >  1:685769/1‑103 (MQ=255)
                                                                                                                   cAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGaa                                                                           >  1:685985/1‑103 (MQ=255)
                                                                                                                   cAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGaa                                                                           <  2:685769/103‑1 (MQ=255)
                                                                                                                   cAGCGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGaa                                                                           <  2:685985/103‑1 (MQ=255)
                                                                                                                      cGGCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGcc                                                                                                                <  3:479137/63‑1 (MQ=255)
                                                                                                                       ggCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGGGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTgg                             <  2:230051/145‑1 (MQ=255)
                                                                                                                       ggCAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTgg                             >  1:230051/1‑145 (MQ=255)
                                                                                                                         cAAACTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGa                      >  2:1250863/1‑150 (MQ=255)
                                                                                                                           aaCTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGaaa                    >  2:259853/1‑150 (MQ=255)
                                                                                                                            aCTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGAt                                 <  4:431607/136‑1 (MQ=255)
                                                                                                                            aCTCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGAt                                 >  3:431607/1‑136 (MQ=255)
                                                                                                                              tCGATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAAc                 <  4:393983/150‑1 (MQ=255)
                                                                                                                                gATTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAActg               <  1:646666/150‑1 (MQ=255)
                                                                                                                                 aTTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAActgc              <  1:958118/150‑1 (MQ=255)
                                                                                                                                 aTTGCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGGTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAActgc              >  2:1110456/1‑150 (MQ=255)
                                                                                                                                    gCGTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGAGCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGAGAAAAACTGCTGa           <  1:147511/150‑1 (MQ=255)
                                                                                                                                      gTGATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATg         >  3:449691/1‑150 (MQ=255)
                                                                                                                                        gATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACcgc                                                      <  1:1314418/103‑1 (MQ=255)
                                                                                                                                        gATCCTCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCt       >  2:265656/1‑150 (MQ=255)
                                                                                                                                             tCGAGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAg  >  4:765017/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAG  >  NC_000913_3_pae_pgi/4158785‑4159075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: