Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 3,486,599 C→A H160Q (CAC→CAA crp → cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi3,486,5990CA97.7% 461.9 / 0.6 134H160Q (CAC→CAAcrpcAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (60/70);  total (61/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 135 total aligned reads displayed.

CCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCAC  >  NC_000913_3_pae_pgi/3486450‑3486745
                                                                                                                                                     |                                                                                                                                                  
   gACATTCTGATGCGTTTGTCTGCACNGATGGCGCGTCGNCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCg                                                                                                                                                 >  3:781958/1‑150 (MQ=255)
   gACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCg                                                                                                                                                 >  1:1629763/1‑150 (MQ=255)
                 tttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAAt                                                                                                                                   <  1:189484/150‑1 (MQ=255)
                    gTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCaa                                                                                                                                <  3:626656/150‑1 (MQ=255)
                     tCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCGGACGCTATGACTCAACCGGACGGTATGCAAATCaaa                                                                                                                               <  3:1029054/150‑1 (MQ=255)
                       tGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAAt                                                                                                                             >  3:679594/1‑150 (MQ=255)
                        gCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCGTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAAtt                                                                                                                            >  1:1264817/1‑150 (MQ=255)
                        gCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAAtt                                                                                                                            >  1:614832/1‑150 (MQ=255)
                         cacaGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTa                                                                                                                           >  1:1113496/1‑150 (MQ=255)
                          acaGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTAc                                                                                                                          <  3:1010816/150‑1 (MQ=255)
                          acaGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTAc                                                                                                                          <  2:708399/150‑1 (MQ=255)
                          acaGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTAc                                                                                                                          >  2:321834/1‑150 (MQ=255)
                                ggCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCa                                                                                                                    <  4:516236/150‑1 (MQ=255)
                                ggCGCGTCGCCGGCAAGTCACTTCGGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCa                                                                                                                    <  2:572813/150‑1 (MQ=255)
                                 gcgcGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCACGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAg                                                                                                                   >  2:983531/1‑150 (MQ=255)
                                   gcgTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGa                                                                                                                 >  2:962314/1‑150 (MQ=255)
                                     gtcgtcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGaaa                                                                                                               <  2:211235/150‑1 (MQ=255)
                                        gtcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTg                                                                                                            <  1:1597205/150‑1 (MQ=255)
                                          cTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGt                                                                                                          <  2:1455293/150‑1 (MQ=255)
                                          cTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTAccc                                                                                                                        <  3:815980/136‑1 (MQ=255)
                                          cTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTAccc                                                                                                                        >  4:815980/1‑136 (MQ=255)
                                           tGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTc                                                                                                         >  2:1420739/1‑150 (MQ=255)
                                            gCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCa                                                                                                        <  2:804794/150‑1 (MQ=255)
                                            gCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCa                                                                                                        <  2:472055/150‑1 (MQ=255)
                                             cAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAg                                                                                                       >  4:415946/1‑150 (MQ=255)
                                              aaGTCACTTCAGAGAAAGGGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGa                                                                                                      <  2:753301/150‑1 (MQ=255)
                                               aGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGAt                                                                                                     <  3:783996/150‑1 (MQ=255)
                                                gTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGAtt                                                                                                    >  3:19167/1‑150 (MQ=255)
                                                 tCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTg                                                                                                   <  1:1109402/150‑1 (MQ=255)
                                                 tCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTg                                                                                                   >  2:1186486/1‑150 (MQ=255)
                                                     ttCCGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg                                                                                               >  4:810496/1‑150 (MQ=255)
                                                      tCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGc                                                                                              >  1:1277013/1‑150 (MQ=255)
                                                       gagagAAAGGGGGCAAACTGGCGGTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCt                                                                                             <  4:781958/149‑1 (MQ=255)
                                                        agagAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTg                                                                                            >  3:829451/1‑150 (MQ=255)
                                                         gagaAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGt                                                                                           <  1:1145254/150‑1 (MQ=255)
                                                         gagaAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGt                                                                                           >  3:554564/1‑150 (MQ=255)
                                                         gagaAAGTGGGCAACCTGGCGTTCATCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGt                                                                                                          >  2:1688305/1‑135 (MQ=255)
                                                         gagaAAGTGGGCAACCTGGCGTTCATCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGt                                                                                                          <  1:1688305/135‑1 (MQ=255)
                                                          agaAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCGGATTGTCGGCTGtt                                                                                          <  1:962314/150‑1 (MQ=255)
                                                          agaAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGtt                                                                                          >  1:920198/1‑150 (MQ=255)
                                                          agaAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGAAAATCAAAATTACCCGCCAGGAAATTGGTCCGTTTGTCGGCTGtt                                                                                          <  4:406660/150‑1 (MQ=255)
                                                            aaaGGGGGCAACCTGGCGTGCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtct                                                                                        <  2:1020606/150‑1 (MQ=255)
                                                               gTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGt                                                                                     >  1:112607/1‑150 (MQ=255)
                                                                  ggCAAGCTGGCGTTCCACGACGTGACGGGCCGCATTGCACAGGCGCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGACAGGAAATTGGACAGATTGTCGGCTGTTCACGTGaa                                                                                  >  4:518941/1‑150 (MQ=255)
                                                                   gCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGaaa                                                                                 >  1:1505954/1‑150 (MQ=255)
                                                                     aaCCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAAcc                                                                               >  1:1555931/1‑150 (MQ=255)
                                                                     aaCCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGATGAATCTGCCAAAACAACAAGACGCAATGACTCATCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAAcc                                                                               <  1:1429428/150‑1 (MQ=255)
                                                                       ccTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGt                                                                             <  1:956596/150‑1 (MQ=255)
                                                                       ccTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGt                                                                             <  1:797771/150‑1 (MQ=255)
                                                                         tGGCGTTCCTCGACCTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAATCGGACGGTATAAAAAACAAAATTAACAGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTgg                                                                           <  2:396288/150‑1 (MQ=255)
                                                                                ccTCGGCGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAt                                                                    <  2:425817/150‑1 (MQ=255)
                                                                                   cGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCt                                                                 >  2:498749/1‑150 (MQ=255)
                                                                                     aCGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGa                                                               <  2:614832/150‑1 (MQ=255)
                                                                                      cGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGaa                                                              <  1:1671100/150‑1 (MQ=255)
                                                                                      cGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGaa                                                              <  3:849284/150‑1 (MQ=255)
                                                                                      cGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGaa                                                              <  2:1398131/150‑1 (MQ=255)
                                                                                      cGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACAAGACGCTATGACTCAACAGGACGGCATGCAACTCAACATTCCCCCCCAGGAAATTGGTCAGCTGGTCTGCTGTGCGCGTGTAGCCGTGGGACTCATTCTGaa                                                              <  2:579646/150‑1 (MQ=255)
                                                                                           cGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGc                                                         <  2:912797/150‑1 (MQ=255)
                                                                                             ggCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGCCTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTg                                                       <  2:975499/150‑1 (MQ=255)
                                                                                             ggCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTg                                                       <  4:554564/150‑1 (MQ=255)
                                                                                                cGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTATCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGaa                                                    <  2:1079876/150‑1 (MQ=255)
                                                                                                cGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGaa                                                    <  1:998389/150‑1 (MQ=255)
                                                                                                    ttGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc                                                <  3:901312/150‑1 (MQ=255)
                                                                                                    ttGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc                                                <  3:415946/150‑1 (MQ=255)
                                                                                                       cacaGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGaa                                                              <  1:42756/133‑1 (MQ=255)
                                                                                                       cacaGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGaa                                                              >  2:42756/1‑133 (MQ=255)
                                                                                                       cacaGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGa                                             >  4:1026448/1‑150 (MQ=255)
                                                                                                       cacaGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGa                                             >  4:1026540/1‑150 (MQ=255)
                                                                                                       cacaGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGa                                             >  1:263995/1‑150 (MQ=255)
                                                                                                        acaGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGt                                                                                                                                           >  1:46158/1‑55 (MQ=255)
                                                                                                        acaGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGt                                                                                                                                           <  2:46158/55‑1 (MQ=255)
                                                                                                        acaGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGaa                                            <  3:161127/150‑1 (MQ=255)
                                                                                                        acaGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGaa                                            >  3:508523/1‑150 (MQ=255)
                                                                                                         caGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAAc                                           <  3:521039/150‑1 (MQ=255)
                                                                                                           gACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCt                                         <  4:679594/150‑1 (MQ=255)
                                                                                                              tctGCTGAATCTGGCAAAACAACCCGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAt                                      <  2:1264817/150‑1 (MQ=255)
                                                                                                              tctGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAt                                      <  2:1561239/150‑1 (MQ=255)
                                                                                                                tgctgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGa                                                  >  4:509475/1‑136 (MQ=255)
                                                                                                                tgctgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAtct                                    >  1:1341527/1‑150 (MQ=255)
                                                                                                                 gctgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTTGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGAtctc                                   <  1:572403/150‑1 (MQ=255)
                                                                                                                 gctgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCACGTGCCCCAGTGCGATGCATTCTGAAGATGCTGGAAGATCAGAACCTGAtctc                                   <  2:920198/150‑1 (MQ=255)
                                                                                                                    gAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGc                                <  1:1186486/150‑1 (MQ=255)
                                                                                                                       tCTGGCAAAACAACCAGACGCTATGAATCAACCGGACGGTATGCAAATCAAACTTACCCGCCCGGAAATTCGTCAGATTGTCGGCTGTTATCGTGACACCGTGGGACGCACTCTGAAGATGCTGGAAGGTCAGGACGTGATCTCCGcaca                             >  2:120559/1‑150 (MQ=255)
                                                                                                                         tGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACg                           <  1:713910/150‑1 (MQ=255)
                                                                                                                         tGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACg                           >  2:1426188/1‑150 (MQ=255)
                                                                                                                         tGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACg                           >  1:124160/1‑150 (MQ=255)
                                                                                                                          ggCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACgg                          >  4:318227/1‑150 (MQ=255)
                                                                                                                                  aacCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCa                  >  3:917718/1‑150 (MQ=255)
                                                                                                                                   acCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAAccct                 <  4:261125/150‑3 (MQ=255)
                                                                                                                                    ccAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAtc                <  2:433617/150‑1 (MQ=255)
                                                                                                                                    ccAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAtc                <  4:19167/150‑1 (MQ=255)
                                                                                                                                    ccAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAtc                >  4:259607/1‑150 (MQ=255)
                                                                                                                                       gACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAtcgtc             <  1:1426188/150‑1 (MQ=255)
                                                                                                                                              tGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACg      <  3:259607/150‑1 (MQ=255)
                                                                                                                                              tGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACg      <  2:681134/150‑1 (MQ=255)
                                                                                                                                                aCTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGc    <  2:1113496/150‑1 (MQ=255)
                                                                                                                                                  tCAACCGGACGGTATGCAAATCAAAATTACCCGTc                                                                                                                     >  2:185657/1‑35 (MQ=255)
                                                                                                                                                  tCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTa        >  2:688745/1‑144 (MQ=255)
                                                                                                                                                  tCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTa        <  1:688745/144‑1 (MQ=255)
                                                                                                                                                  tCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCAc  >  4:100331/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCAC  >  NC_000913_3_pae_pgi/3486450‑3486745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: