Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,376,8660TG35.8% 52.0 / 92.2 81V12G (GTC→GGC) ycjSputative NADH‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (31/21);  new base G (16/13);  total (47/34)
Fisher's exact test for biased strand distribution p-value = 8.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.02e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCGGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTGGCAGAAAAATACGGGAATGCATCCGTGTG  >  NC_000913_3_pae_pgi/1376721‑1377013
                                                                                                                                                 |                                                                                                                                                   
gcgGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGcgcg                                                                                                                                                 >  1:220827/1‑150 (MQ=255)
 cgGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTTGTTGCGTACCTGATAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGa                                                                                                                                                <  2:533809/150‑1 (MQ=255)
 cgGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTACGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGa                                                                                                                                                >  2:677460/1‑150 (MQ=255)
 cgGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGa                                                                                                                                                <  4:102027/150‑1 (MQ=255)
       aCTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAg                                                                                                                                          <  1:243853/150‑1 (MQ=255)
        cTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACCAGCTCTCCGCTGCGGGTCGCGATAATCgg                                                                                                                                         >  2:384893/1‑150 (MQ=255)
        cTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAgg                                                                                                                                         >  3:264855/1‑150 (MQ=255)
        cTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAgg                                                                                                                                         >  3:231731/1‑150 (MQ=255)
         tATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGgc                                                                                                                                        >  2:584134/1‑150 (MQ=255)
           tCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGgcgc                                                                                                                                      >  2:728167/1‑150 (MQ=255)
               ggCTGTGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGc                                                                                                                                  <  2:21621/150‑1 (MQ=255)
                      tAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTgg                                                                                                                           >  3:80780/1‑150 (MQ=255)
                      tAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTgg                                                                                                                           >  1:851043/1‑150 (MQ=255)
                      tAGTGCACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTgg                                                                                                                           <  4:192272/150‑1 (MQ=255)
                         tgtACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCgg                                                                                                                        >  1:72416/1‑150 (MQ=255)
                         tgtACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCgg                                                                                                                        >  2:182254/1‑150 (MQ=255)
                          gtACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGCGGCGGa                                                                                                                       >  3:286805/1‑150 (MQ=255)
                               aaGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAgg                                                                                                                  >  4:206671/1‑150 (MQ=255)
                                 gggCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGtt                                                                                                                >  1:72088/1‑150 (MQ=255)
                                    cGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCAt                                                                                                             <  1:288353/150‑1 (MQ=255)
                                      tATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGc                                                                                                           >  1:569752/1‑150 (MQ=255)
                                          cGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCg                                                                                                       >  4:570243/1‑150 (MQ=255)
                                          cGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCg                                                                                                       >  1:806253/1‑150 (MQ=255)
                                           gggCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGACAAGTGCCATGACCAGATCTCCGCTGAGGCTCGCGATACTAGGCGCCGGCCAGGTGGCGGATAAGGTTCATGCTTCGt                                                                                                      <  3:226008/150‑1 (MQ=255)
                                                gAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGtactac                                                                                                 >  3:372302/1‑150 (MQ=255)
                                                      ccTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCAcc                                                                                           >  3:39768/1‑150 (MQ=255)
                                                       cTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCAccc                                                                                          >  3:409282/1‑150 (MQ=255)
                                                        tGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCCTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCg                                                                                         >  3:461658/1‑150 (MQ=255)
                                                            cAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAAc                                                                                     <  1:155015/150‑1 (MQ=255)
                                                             aGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACg                                                                                    >  2:631729/1‑150 (MQ=255)
                                                                  tACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTg                                                                               <  2:72573/150‑1 (MQ=255)
                                                                     cGTGATTCGTTGGCCATGTTGCGTACCTGTTAAAAGTTCTCTGTGAAACGAGCACTGACAAGCTCTCCGCTGCGGGGCGCGATAAAAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCTTACCACTGCACCCTCAACGATCTTGag                                                                            <  2:558801/150‑2 (MQ=255)
                                                                       tGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACt                                                                          <  3:555149/150‑1 (MQ=255)
                                                                       tGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACt                                                                          <  3:491130/150‑1 (MQ=255)
                                                                         aTTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACtgg                                                                        <  2:519697/150‑1 (MQ=255)
                                                                           tCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTATCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACtggtg                                                                      >  1:511790/1‑150 (MQ=255)
                                                                            cGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACtggtgg                                                                     <  2:220827/150‑1 (MQ=255)
                                                                             gTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGc                                                                    >  2:830912/1‑150 (MQ=255)
                                                                              ttGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGct                                                                   <  1:285946/150‑1 (MQ=255)
                                                                                ggCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGctgt                                                                 <  3:500210/150‑1 (MQ=255)
                                                                                 gCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGctgtc                                                                >  3:311765/1‑150 (MQ=255)
                                                                                     ggTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGTGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCtgtg                                                            >  3:319973/1‑150 (MQ=255)
                                                                                      gTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGctgtcacaga                                                           <  4:231731/150‑6 (MQ=255)
                                                                                      gTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGa                                                           >  3:276472/1‑150 (MQ=255)
                                                                                             aCCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAgccgc                                                    >  2:33095/1‑150 (MQ=255)
                                                                                             aCCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAgccgc                                                    >  2:716676/1‑150 (MQ=255)
                                                                                             aCCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAgccgc                                                    <  4:448311/150‑1 (MQ=255)
                                                                                               cTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGAGAAGGGTCATGCTGCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCt                                                  >  3:401679/1‑150 (MQ=255)
                                                                                               cTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCt                                                  >  3:405877/1‑150 (MQ=255)
                                                                                               cTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCt                                                  <  4:492851/150‑1 (MQ=255)
                                                                                                tGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATc                                                                                 <  1:45200/118‑1 (MQ=255)
                                                                                                tGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATc                                                                                 >  2:45200/1‑118 (MQ=255)
                                                                                                tGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAgccgccag                                                 <  1:653799/150‑3 (MQ=255)
                                                                                                 gCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCttt                                                >  3:198188/1‑150 (MQ=255)
                                                                                                  cTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTc                                               <  2:492718/150‑1 (MQ=255)
                                                                                                  cTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTc                                               <  1:745758/150‑1 (MQ=255)
                                                                                                   tAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTcc                                              >  2:841101/1‑150 (MQ=255)
                                                                                                    aaGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTccc                                             <  2:72088/150‑1 (MQ=255)
                                                                                                      gagGTCTTTGTGAAAAGTGCAATAACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCcag                                           <  1:676557/150‑1 (MQ=255)
                                                                                                       agGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACtggt                                                                       <  2:656081/121‑1 (MQ=255)
                                                                                                       agGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACtggt                                                                       >  1:656081/1‑121 (MQ=255)
                                                                                                         gTCTTTGTGAAAAGTGCAAGGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCCGGGGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCCGTCTCGGACAGCCGCCTTTCCcaggcg                                        >  2:668161/1‑150 (MQ=255)
                                                                                                          tCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTAATACTGCATCAGTCCAGATCTGCCATTGGTGGCTGTCCGTGAACGCCGCCTTTCCcagccgc                                       >  1:753793/1‑150 (MQ=255)
                                                                                                           cTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCcaggcgca                                      >  1:484726/1‑150 (MQ=255)
                                                                                                            tttGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCcaggcgcag                                     <  1:878225/150‑1 (MQ=255)
                                                                                                                  aaaaGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTg                               <  4:57059/150‑1 (MQ=255)
                                                                                                                  aaaaGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTg                               >  3:57059/1‑150 (MQ=255)
                                                                                                                  aaaaGTGCAATGACAAGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTg                               >  1:261116/1‑150 (MQ=255)
                                                                                                                   aaaGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTgg                              <  4:80780/150‑1 (MQ=255)
                                                                                                                    aaGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTGGc                             >  4:83776/1‑150 (MQ=255)
                                                                                                                              aCAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTGGCAGAAAAATAc                   <  2:479517/150‑1 (MQ=255)
                                                                                                                                aaGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTGGCAGAAAAATACgg                 <  4:286805/150‑1 (MQ=255)
                                                                                                                                 aGCTCTCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTGGCAGAAAAATACggg                >  1:722862/1‑150 (MQ=255)
                                                                                                                                   ctctCCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTGGCAGAAAAATACGGGaa              >  1:604073/1‑150 (MQ=255)
                                                                                                                                    tctcCGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCCGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTGGCAGAAAAATACGGGAAt             >  1:427485/1‑150 (MQ=255)
                                                                                                                                     ctcCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTGGCAGAAAAATACGGGAATg            >  2:740304/1‑150 (MQ=255)
                                                                                                                                      tcCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTGGAAGCACAATACGGGAATGc           >  2:739196/1‑150 (MQ=255)
                                                                                                                                        cGCTGCGGGGCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTCCTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTGGCAGAAAAATACGGGAATGCAt         >  1:719601/1‑150 (MQ=255)
                                                                                                                                         gCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTGGCAGAAAAATACGGGAATGCATc        <  1:631729/150‑1 (MQ=255)
                                                                                                                                              gggTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTGGCAGAAAAATACGGGAATGCATCCgtgt   <  1:584134/150‑1 (MQ=255)
                                                                                                                                               ggTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTGGCAGAAAAATACGGGAATGCATCCGtgtg  <  2:511790/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCGGATAACTATCAGGGCTATGTAGTGTACGAAGGGCGTATCCGGGCGGAAGATCCTGCCCAGGCGTACCGTGATTCGTTGGCCTGGTTGCGTACCTGCTAAGAGGTCTTTGTGAAAAGTGCAATGACAAGCTCTCCGCTGCGGGTCGCGATAATAGGCGCAGGCCAGGTGGCGGATAAGGTTCATGCTTCGTACTACTGCACCCGCAACGATCTGGAACTGGTGGCTGTCTGTGACAGCCGCCTTTCCCAGGCGCAGGCGCTGGCAGAAAAATACGGGAATGCATCCGTGTG  >  NC_000913_3_pae_pgi/1376721‑1377013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑