Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi4,295,3200CT31.4% 118.5 / 62.4 87intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (34/25);  new base T (17/10);  total (52/35)
Fisher's exact test for biased strand distribution p-value = 8.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGC  >  NC_000913_3_pae_pgi/4295173‑4295466
                                                                                                                                                    |                                                                                                                                                  
tCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc                                                                                                                                                  <  1:298436/150‑1 (MQ=32)
  ggATAAGGCGTTTATGCCGCATCCGACATCAACGCATGATGCGACGCTTAACGCGTCTTATCCGGCCTACGACAGGCTGTGCGTTAGACTGCTTTAGCCTGACTTTTTAGATTGTATATCGCATTTACGCTGCATCAGACATCAATGCCt                                                                                                                                                >  1:803020/1‑150 (MQ=2)
           gTTTATCCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACg                                                                                                                                       >  2:786861/1‑150 (MQ=17)
                tGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTTTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATGTAGCGTGATTTTGTAGGTCGGATAAGGCGATTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGc                                                                                                                                  <  1:877694/150‑1 (MQ=255)
                 gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGGAATAGCCTGATTGAGCGTGATTTTGTAGGTCGGAGAAGGCGTGTACGCCGCATCCGGCAGCAACGCCTGAAGCGACGCTTGcc                                                                                                                                 >  4:262614/1‑150 (MQ=11)
                 gCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGcc                                                                                                                                 <  1:37324/150‑1 (MQ=35)
                   cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgc                                                                                                                               >  4:373129/1‑150 (MQ=21)
                   cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgc                                                                                                                               >  2:176558/1‑150 (MQ=21)
                   cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgc                                                                                                                               <  3:366924/150‑1 (MQ=21)
                     cgcactgcATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCCTCCGACATCAACGCCTGATGCGACGCTTGCCGCGt                                                                                                                             <  2:294834/143‑1 (MQ=255)
                     cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt                                                                                                                             >  1:88102/1‑150 (MQ=37)
                      aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc                                                                                                                            >  1:832167/1‑150 (MQ=25)
                        ccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                          >  3:319127/1‑150 (MQ=21)
                          gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGGAATAGCCGGAGGGATCAGGATTTTTGTCGGTGCGGAAGTCCGTTACGCCGCATCCGACATCAACGCCGTATGCGATTCTTTCCGCGTCttcg                                                                                                                        >  1:668286/1‑148 (MQ=2)
                               gtaggCCTGATAAGACGCGTTAAGCGTCGCATCAGGCATACGCCAGACAGCGCAATGGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                   >  2:574586/5‑150 (MQ=11)
                                aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                                                                                                                                                    >  1:229632/1‑116 (MQ=33)
                                aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                  >  4:393451/1‑150 (MQ=25)
                                 aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt                                                                                                                 <  3:101577/150‑1 (MQ=38)
                                 aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgc                                                                                                                               <  2:698617/136‑1 (MQ=19)
                                 aCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCcgc                                                                                                                               >  1:698617/1‑136 (MQ=25)
                                   gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat                                                                                                               >  1:143899/1‑149 (MQ=18)
                                   gCCTGATAAGACGCGTTAAGCGTCGCATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTat                                                                                                               >  2:858696/1‑149 (MQ=11)
                                     cTGATCCGAAGCTTAACGTGTCTTATGAGGCCTAAGTCATGCGGAACAATGGTAAGACTTAGCGTCATTTAGTAAATCGGACAAGGCGTTTCCGCCGCATACGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGc                                                                                                             <  2:143465/150‑1 (MQ=2)
                                      tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctt                                                                                                            >  2:18463/1‑146 (MQ=17)
                                       gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatctta                                                                                                           >  1:74003/1‑145 (MQ=2)
                                       gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCa                                                                                                           <  3:13715/150‑1 (MQ=39)
                                            gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCTAGACAg                                                                                                      >  1:4494/1‑150 (MQ=38)
                                               gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCt                                                                                                                                                <  3:184457/105‑1 (MQ=33)
                                                    aCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGa                                                                                                         >  3:380270/1‑139 (MQ=17)
                                                     cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                                             <  1:396227/150‑1 (MQ=38)
                                                      gcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGcc                                                                                            <  1:694944/150‑1 (MQ=39)
                                                        gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                                                                          <  4:333698/150‑1 (MQ=39)
                                                          cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGc                                                                                                                                                  <  2:346871/92‑1 (MQ=34)
                                                          cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg                                                                                                                                            >  1:780409/1‑98 (MQ=33)
                                                          cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg                                                                                                                                            <  2:780409/98‑1 (MQ=34)
                                                            tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                          >  4:110449/1‑114 (MQ=9)
                                                            tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                          <  3:110449/114‑1 (MQ=25)
                                                             aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGcc                                                                                                                                                 <  2:221233/90‑1 (MQ=18)
                                                             aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACAGCAATGcc                                                                                                                                                 >  1:221233/1‑90 (MQ=255)
                                                               cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc                                                                                   <  3:194044/150‑1 (MQ=39)
                                                                 ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGc                                                                                                                                  >  4:412538/1‑101 (MQ=25)
                                                                 ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGc                                                                                                                                  <  3:412538/101‑1 (MQ=25)
                                                                  gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAAGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATATCCTGATTTAGCGTg                                                                                >  3:477045/1‑150 (MQ=255)
                                                                    cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAt                                                                              >  3:59794/1‑150 (MQ=39)
                                                                    cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAAGACCGAAAAGGCGTCTTGGCGGGATCGAACATCAAGGAGTGAGTAGACCCCGGCCGCGGCTTATTCGGCCTACGCCAAACAGCGCAATAGCCTGATTTAGCGTGAt                                                                              >  4:262048/1‑150 (MQ=2)
                                                                        gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTg                                                                          >  4:190295/1‑150 (MQ=38)
                                                                        gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACACCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTg                                                                          >  4:17912/1‑150 (MQ=38)
                                                                         ccAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                                                                         <  1:190533/150‑1 (MQ=39)
                                                                               aGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCg                                                                   <  4:114433/150‑1 (MQ=38)
                                                                                  gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAt                                                                >  2:667178/1‑150 (MQ=38)
                                                                                  gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCGTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAt                                                                <  1:247075/150‑1 (MQ=37)
                                                                                      tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                     <  2:652478/93‑1 (MQ=255)
                                                                                      tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                     >  1:652478/1‑93 (MQ=255)
                                                                                         ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGt                                                         >  3:431327/1‑150 (MQ=255)
                                                                                          cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGtt                                                        >  4:250128/1‑150 (MQ=38)
                                                                                           tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACACCGCATCCGACATCAACGCCTGATGCGACGCtt                                                                                                                                    >  4:350364/1‑73 (MQ=255)
                                                                                           tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACACCGCATCCGACATCAACGCCTGATGCGACGCtt                                                                                                                                    <  3:350364/73‑1 (MQ=25)
                                                                                            gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTa                                                      <  1:269369/150‑1 (MQ=38)
                                                                                                tAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc                                                  <  3:476559/150‑1 (MQ=39)
                                                                                                tAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGAGGCGATTGACGCGTCTTATAAGGCCTACGACAGCCCGCGCAATAGAATGATTTGGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc                                                  <  3:371799/150‑1 (MQ=11)
                                                                                                tAGCGTGAATTAGTAGGACGGCCAAGGCGTTTACGCCCCGTACGACGTCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACTCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc                                                  <  4:382844/150‑1 (MQ=11)
                                                                                                 aGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGACAGCGCAATAGCCTGCTTTCGCGTGATTTTGTAGGTCGGATCCGGCGTTTCTGCCg                                                 >  2:746801/1‑150 (MQ=14)
                                                                                                   cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCtt                                                                                                                                    <  1:103671/65‑1 (MQ=255)
                                                                                                   cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCtt                                                                                                                                    >  2:103671/1‑65 (MQ=37)
                                                                                                    gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTAATGCCGCAt                                              >  4:535363/1‑150 (MQ=37)
                                                                                                      gATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATcc                                            <  1:144412/150‑1 (MQ=37)
                                                                                                       aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                                                                             >  4:98602/1‑68 (MQ=255)
                                                                                                       aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                                                                             <  3:98602/68‑1 (MQ=255)
                                                                                                             tgaGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATc                                      >  1:360793/3‑150 (MQ=38)
                                                                                                             gTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATc                                      >  1:818307/1‑150 (MQ=39)
                                                                                                             gTAGGTCGGATAAGGCGTTTACACCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATc                                      <  3:17912/150‑1 (MQ=38)
                                                                                                                ggTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                                                                                                    >  3:84166/1‑69 (MQ=16)
                                                                                                                 gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTATATGCCGAATCCGACATCAACg                                  >  1:29978/1‑150 (MQ=255)
                                                                                                                     gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGTCGTTTACGCCGCAGCCGACCTCAACCCCTg                              >  4:559403/1‑150 (MQ=11)
                                                                                                                      aTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGt                                                                     <  2:126471/110‑1 (MQ=40)
                                                                                                                      aTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGt                                                                     >  1:126471/1‑110 (MQ=40)
                                                                                                                        aaGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATg                           <  1:667178/150‑1 (MQ=39)
                                                                                                                          ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAg                                                                                                      <  2:519242/73‑1 (MQ=255)
                                                                                                                          ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAg                                                                                                      >  1:519242/1‑73 (MQ=255)
                                                                                                                           gCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTGTGCCGCATCCGACATCAACGCCCGCTGCgg                        >  4:252469/1‑149 (MQ=255)
                                                                                                                                 aCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCAGATCGGACCTACGCCCGTCAGCGCTCCAGCCAGGTTTAACGTGATCTTGTACGTCGGATAAGTCGAAAAAAACGCATCCGACATCAACGCCTGATGCGACGCTTa                  >  1:39726/1‑150 (MQ=2)
                                                                                                                                   gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAc                >  2:321538/1‑150 (MQ=35)
                                                                                                                                   gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCACGTCGGATCAGGCCTGCGCCGGGCAGCGGAATAGCCCGGTTTGTCGATATTGTGTAGCGCGGAAAAGGCGATTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAc                >  2:877694/1‑150 (MQ=11)
                                                                                                                                    ccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcg               >  1:764382/1‑150 (MQ=25)
                                                                                                                                     cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgc              >  1:510164/1‑150 (MQ=35)
                                                                                                                                      gcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAcgcg             >  3:420063/1‑150 (MQ=35)
                                                                                                                                         tCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCt          >  4:306207/1‑150 (MQ=35)
                                                                                                                                                 cAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc  >  4:134288/1‑150 (MQ=32)
                                                                                                                                                    |                                                                                                                                                  
TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGC  >  NC_000913_3_pae_pgi/4295173‑4295466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: