BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_pgi	3,440,128	G→A	T301I (ACT→ATT)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_pgi	3,440,128	0	G	A	100.0%	408.8 / NA	115	T301I (ACT→ATT)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (59/56); total (59/56)

Only 100 of 115 total aligned reads displayed.

ACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCA  >  NC_000913_3_pae_pgi/3439982‑3440272

aCTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATaa                                                                                                                                               >  4:611525/1‑150 (MQ=255)

    ttACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAgaga                                                                                                                                           >  4:126400/1‑150 (MQ=255)

    ttACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAgaga                                                                                                                                           >  4:39579/1‑150 (MQ=255)

    ttACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAAACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAgaga                                                                                                                                           >  4:719330/1‑150 (MQ=255)

        gATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGCtttt                                                                                                                                       >  2:1080663/1‑150 (MQ=255)

        gATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGAtttt                                                                                                                                       >  3:579833/1‑150 (MQ=255)

        gATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGACGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGAtgtt                                                                                                                                       >  1:755317/1‑150 (MQ=255)

           ggCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGGGATTTTTTa                                                                                                                                    <  3:591364/150‑1 (MQ=255)

           ggCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTa                                                                                                                                    <  3:84115/150‑1 (MQ=255)

            gcgcATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTAc                                                                                                                                   >  3:748610/1‑150 (MQ=255)

              gcgtGACCTTATCCTGCGCAGTAAAACCGTAACCTGCGATCCGGTTACTCGTCAGCGATGCTTGCCGGGGGCCTGTTTTCCAGGCGCATGCCCAGAGACATTCCACAGGAAGCCAGCACGTCTTTAATCTCAAGAAGAGATTTTTTACCa                                                                                                                                 >  4:164331/4‑150 (MQ=255)

              gcATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCa                                                                                                                                 >  1:1232816/1‑150 (MQ=255)

              gcATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCa                                                                                                                                 >  1:1119805/1‑150 (MQ=255)

               cATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCaa                                                                                                                                <  2:284895/150‑1 (MQ=255)

                 tGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGTCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAgg                                                                                                                              >  2:1109136/1‑150 (MQ=255)

                   aCCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATaa                                                                                                                                               >  1:1136121/1‑131 (MQ=255)

                   aCCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATaa                                                                                                                                               <  2:1136121/131‑1 (MQ=255)

                   aCCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGtt                                                                                                                            <  2:406233/150‑1 (MQ=255)

                   aCCTTATCCTTCTCAGTAAAACCTTAACCTGGGATCCGGTTACTCGTCAGCGAGGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGtt                                                                                                                            <  2:765645/150‑1 (MQ=255)

                     cTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAg                                                                                                                          >  4:620258/1‑150 (MQ=255)

                           cTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGttt                                                                                                                    >  4:292926/1‑150 (MQ=255)

                           cTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGttt                                                                                                                    >  3:418599/1‑150 (MQ=255)

                           cTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGttt                                                                                                                    <  1:244112/150‑1 (MQ=255)

                            ttCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTATCAAGGTTAGGCGtttt                                                                                                                   <  3:611525/150‑1 (MQ=255)

                            ttCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGtttt                                                                                                                   >  1:601008/1‑150 (MQ=255)

                            ttCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGtttt                                                                                                                   >  1:769602/1‑150 (MQ=255)

                              ctcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCGAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTaa                                                                                                                 >  1:181188/1‑150 (MQ=255)

                              ctcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTaa                                                                                                                 <  1:1165282/150‑1 (MQ=255)

                               tcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                                                                >  2:959280/1‑150 (MQ=255)

                               tcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                                                                <  4:25066/150‑1 (MQ=255)

                                 aGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGGGATTTTTTACCAAGGTTAGGCGTTTTa                                                                                                                  <  1:884546/146‑1 (MQ=255)

                                 aGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTa                                                                                                                  >  2:884546/1‑146 (MQ=255)

                                 aGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGa                                                                                                              >  1:51343/1‑150 (MQ=255)

                                   tAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                                                                <  4:128988/146‑1 (MQ=255)

                                   tAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGc                                                                                                            <  2:387354/150‑1 (MQ=255)

                                    aaaaCCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCt                                                                                                           <  3:39409/150‑1 (MQ=255)

                                     aaaCCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTc                                                                                                          >  4:522210/1‑150 (MQ=255)

                                        ccTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAAc                                                                                                       >  1:384606/1‑150 (MQ=255)

                                         cTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAAcc                                                                                                      >  3:197315/1‑150 (MQ=255)

                                            caCCTGTGATCCGGTTACTCGTCAGCGGTGCTTGCCGGTGGCGAGTTGTCCAGGCGGATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTCATCTCCATCAGAGATGTTTTACCAAGGTTTGGGCTTTTAAGGAGATCAcccgcc                                                                                                   <  2:137504/149‑4 (MQ=255)

                                               cTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTa                                                                                                <  3:352774/150‑1 (MQ=255)

                                                     tCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGt                                                                                          >  1:455587/1‑150 (MQ=255)

                                                         gTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCa                                                                                      >  3:300485/1‑150 (MQ=255)

                                                           tACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGa                                                                                    <  3:53244/150‑1 (MQ=255)

                                                              tCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGAtttttt                                                                                                                                     <  2:989717/98‑1 (MQ=255)

                                                              tCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGAtttttt                                                                                                                                     >  1:989717/1‑98 (MQ=255)

                                                              tCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCa                                                                                 >  4:228273/1‑150 (MQ=255)

                                                              tCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGACTTTAATCTCAATAAGAGAGTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCa                                                                                 >  3:224161/1‑150 (MQ=255)

                                                               cGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAc                                                                                <  3:182527/150‑1 (MQ=255)

                                                                gTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCaa                                                                                                        >  2:778082/1‑125 (MQ=255)

                                                                gTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCaa                                                                                                        <  1:778082/125‑1 (MQ=255)

                                                                 tCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCg                                                                              >  3:588463/1‑150 (MQ=255)

                                                                 tCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCg                                                                              <  1:325774/150‑1 (MQ=255)

                                                                 tCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCg                                                                              >  1:284730/1‑150 (MQ=255)

                                                                   aGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTCATCTCAATAAGAGATTTTTTACCAAGGGTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTAACAGATCACCGat                                                                            >  1:6537/1‑150 (MQ=255)

                                                                       aTGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCATGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAg                                                                        <  2:432230/150‑1 (MQ=255)

                                                                           ttGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGTCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGa                                                                    <  1:1109136/150‑1 (MQ=255)

                                                                               catgcgCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                                                                >  3:17624/6‑102 (MQ=255)

                                                                               catgcgCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                                                                <  4:17624/97‑1 (MQ=255)

                                                                                    gCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTACTCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTg                                                           >  2:138254/1‑150 (MQ=255)

                                                                                     ccAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGc                                                          <  2:284730/150‑1 (MQ=255)

                                                                                       aGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCtt                                                        <  3:574113/150‑1 (MQ=255)

                                                                                          tttCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTaa                                                     <  2:393692/150‑1 (MQ=255)

                                                                                           ttCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAg                                                    >  4:520769/1‑150 (MQ=255)

                                                                                             ccAGGCGCATGGCCAGGGACAGTCCACGGGAAGCCATCACGTCTTTAATCTCAATATGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGTATAGCTTCTGCTTTAAGGc                                                  <  2:6537/150‑1 (MQ=255)

                                                                                              cAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAGGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCgg                                                                                                  >  3:633552/1‑101 (MQ=255)

                                                                                              cAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCgg                                                                                                  <  4:633552/101‑1 (MQ=255)

                                                                                               aGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAg                                                >  2:166609/1‑150 (MQ=255)

                                                                                                ggCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGt                                               <  3:234249/150‑1 (MQ=255)

                                                                                                ggCGCATGCATAGAACAAGTCCACGGGACGTCAGCCCGTCTTTATTCTCAATAAGGGATTTGTTACCAAGGTTAGGCGTTTTAAGGAGCTCATCCTCGGTACGCTATTACAGATCACCGATATCGTGGATAGCTTCTGCTTTAAGGCAGt                                               <  4:183808/150‑1 (MQ=255)

                                                                                                        cccAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCagag                                       >  1:1130203/1‑150 (MQ=255)

                                                                                                        cccAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCagag                                       <  2:601008/150‑1 (MQ=255)

                                                                                                          cAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGACATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCg                                     >  2:1176822/1‑150 (MQ=255)

                                                                                                            gagaCAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGa                                   <  1:166609/150‑1 (MQ=255)

                                                                                                               aCAGTCCACGGGAAGCCAGCACGTCTTTAATCTAAATAAGAGATTTTTTACAAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCCGTTAGCAGAGCGGACAg                                >  2:873205/1‑150 (MQ=255)

                                                                                                                 aGTCCACGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGata                                                                           <  1:709765/105‑1 (MQ=255)

                                                                                                                      aCGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCATGGTTATGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATACAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTc                         >  2:866802/1‑150 (MQ=255)

                                                                                                                      aCGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTc                         <  2:129913/150‑1 (MQ=255)

                                                                                                                       cGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTcc                        <  4:588463/150‑1 (MQ=255)

                                                                                                                       cGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTcc                        <  1:959280/150‑1 (MQ=255)

                                                                                                                       cGGGAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTcc                        <  3:719330/150‑1 (MQ=255)

                                                                                                                          gAAGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGa                     >  3:677386/1‑150 (MQ=255)

                                                                                                                           aaGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAg                                >  4:281892/1‑138 (MQ=255)

                                                                                                                            aGCCAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATc                   <  4:300485/150‑1 (MQ=255)

                                                                                                                               cAGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTc                <  1:468864/150‑1 (MQ=255)

                                                                                                                               cAGCACGTCTTTAATCCCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGTGGACAGTCAATTCCAGATCGTc                >  2:415627/1‑150 (MQ=255)

                                                                                                                                aGCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTGAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTGAGGCAGTTAGAAGAGAGTACAGTCAGTTCAAGATcaggt               >  2:1070834/1‑146 (MQ=255)

                                                                                                                                 gCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCaa                            >  3:362541/1‑136 (MQ=255)

                                                                                                                                 gCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCaa                            <  4:362541/136‑1 (MQ=255)

                                                                                                                                 gCACGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCaa              >  1:355523/1‑150 (MQ=255)

                                                                                                                                   aCGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACa            >  3:303026/1‑150 (MQ=255)

                                                                                                                                   aCGTCTTTAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACa            <  3:292926/150‑1 (MQ=255)

                                                                                                                                     gTCTTTAATCTCAATAAGATATTGTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAgg          <  3:393620/150‑1 (MQ=255)

                                                                                                                                        tttAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGgcg       >  3:159868/1‑150 (MQ=255)

                                                                                                                                         ttAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGTGGACAGTCAATTCCAGATCGTCAACAGGgcgc      <  1:415627/150‑1 (MQ=255)

                                                                                                                                         ttAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGgcgc      >  1:430566/1‑150 (MQ=255)

                                                                                                                                         ttAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGgcgc      >  3:723089/1‑150 (MQ=255)

                                                                                                                                          tAATCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGCGCgca     <  1:337736/150‑1 (MQ=255)

                                                                                                                                            aTCTCAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCgcagc   >  1:1114246/1‑150 (MQ=255)

                                                                                                                                             tctcAATAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCgcagca  <  4:723089/150‑1 (MQ=255)

ACTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCAATTCCAGATCGTCAACAGGGCGCAGCA  >  NC_000913_3_pae_pgi/3439982‑3440272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`