Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 4,159,127 C→A A213E (GCG→GAG)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi4,159,1270CA96.2% 347.2 / 4.7 105A213E (GCG→GAG) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (57/44);  total (58/47)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

Only 100 of 105 total aligned reads displayed.

TGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGC  >  NC_000913_3_pae_pgi/4158983‑4159273
                                                                                                                                                |                                                                                                                                                  
tGTTGATGGCTATCTATGTAGTTCACCAGTGGGCGAACCGCGAGTGAGTACCGATGGACGATCTGGCAGGGGAGAAGGTGCTGATGCTGGAAGATGGTTACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATg                                                                                                                                               <  4:334305/150‑1 (MQ=255)
 gTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGa                                                                                                                                              <  2:1041662/150‑1 (MQ=255)
  ttGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGaa                                                                                                                                             <  3:401669/150‑1 (MQ=255)
  ttGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGaa                                                                                                                                             <  1:1206555/150‑1 (MQ=255)
   tGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAg                                                                                                                                            >  4:222844/1‑150 (MQ=255)
     cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGTAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAAAGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGAt                                                                                                                                          >  1:211197/1‑150 (MQ=255)
      tGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATa                                                                                                                                         >  1:291461/1‑150 (MQ=255)
      tGGCTATCTATGAAGAGCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATa                                                                                                                                         >  2:626346/1‑150 (MQ=255)
        gCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATaca                                                                                                                                       >  1:549578/1‑150 (MQ=255)
           atctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATacacac                                                                                                                                    <  1:557938/150‑1 (MQ=255)
              tatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTc                                                                                                                                 >  2:1106463/1‑150 (MQ=255)
                tGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGGAGATACACACTTCcg                                                                                                                               >  2:869059/1‑150 (MQ=255)
                 gAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCcgc                                                                                                                              >  2:1005742/1‑150 (MQ=255)
                    gATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCcgcgcg                                                                                                                           >  1:908086/1‑150 (MQ=255)
                        aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCa                                                                                                                       >  1:561580/1‑150 (MQ=255)
                          ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGCTCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCatc                                                                                                                     <  4:591810/150‑3 (MQ=255)
                            gTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCt                                                                                                                   >  1:559825/1‑150 (MQ=255)
                                gCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGaa                                                                                                               <  2:69590/150‑1 (MQ=255)
                                 cGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGaaa                                                                                                              >  1:130671/1‑150 (MQ=255)
                                   aaCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAAct                                                                                                            >  1:738169/1‑150 (MQ=255)
                                     ccGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAActct                                                                                                          <  1:420823/150‑1 (MQ=255)
                                      cgcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTg                                                                                                         <  1:1106463/150‑1 (MQ=255)
                                       gcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgc                                                                                                        <  2:561580/150‑1 (MQ=255)
                                        cgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCTATCAGGCAATGGGTGTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgcg                                                                                                       <  2:957760/150‑1 (MQ=255)
                                        cgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgcg                                                                                                       <  2:899094/150‑1 (MQ=255)
                                              cGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAt                                                                                                 <  3:222844/150‑1 (MQ=255)
                                                      tGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcgg                                                                                         <  4:38322/150‑1 (MQ=255)
                                                       ggCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAg                                                                                                                                            >  2:823127/1‑98 (MQ=255)
                                                       ggCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAg                                                                                                                                            <  1:823127/98‑1 (MQ=255)
                                                         ccGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGaa                                                                                                                                             <  3:292512/95‑1 (MQ=255)
                                                         ccGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGaa                                                                                                                                             >  4:292512/1‑95 (MQ=255)
                                                         ccGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg                                                                                      >  4:634201/1‑150 (MQ=255)
                                                          cGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGaaa                                                                                                              <  1:274799/125‑1 (MQ=255)
                                                          cGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGaaa                                                                                                              >  2:274799/1‑125 (MQ=255)
                                                            aTCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTa                                                                                   >  1:770408/1‑150 (MQ=255)
                                                            aTCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTa                                                                                   >  4:157724/1‑150 (MQ=255)
                                                               tGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCg                                                                                <  1:700852/150‑1 (MQ=255)
                                                               tGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCg                                                                                <  1:470844/150‑1 (MQ=255)
                                                               tGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCg                                                                                >  4:688861/1‑150 (MQ=255)
                                                                ggCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCgg                                                                               <  1:196589/150‑1 (MQ=255)
                                                                 gCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCggg                                                                              >  2:686432/1‑150 (MQ=255)
                                                                   aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAt                                                                            >  2:412126/1‑150 (MQ=255)
                                                                   aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAt                                                                            >  1:409435/1‑150 (MQ=255)
                                                                   aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATAAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAt                                                                            >  1:769774/1‑150 (MQ=255)
                                                                    ggggAAAAACTGCTGATGCTGGAAGATGGTCACTTTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATc                                                                           <  2:549578/150‑1 (MQ=255)
                                                                     gggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCa                                                                          >  4:613467/1‑150 (MQ=255)
                                                                      ggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAc                                                                         >  4:231984/1‑150 (MQ=255)
                                                                      ggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAc                                                                         >  2:293627/1‑150 (MQ=255)
                                                                       gAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACt                                                                        <  1:468863/150‑1 (MQ=255)
                                                                       gAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACt                                                                        >  3:530153/1‑150 (MQ=255)
                                                                       gAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACt                                                                        >  4:681214/1‑150 (MQ=255)
                                                                        aaaaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACtt                                                                       <  4:12074/150‑1 (MQ=255)
                                                                         aaaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACttt                                                                      <  3:212567/150‑1 (MQ=255)
                                                                            aCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACt                                                                   <  1:104326/150‑1 (MQ=255)
                                                                              tgctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGc                                                                 <  2:409435/150‑1 (MQ=255)
                                                                               gctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGcc                                                                >  2:630080/1‑150 (MQ=255)
                                                                                ctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCa                                                               >  4:252277/1‑150 (MQ=255)
                                                                                   aTGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCaa                                                                                                    >  2:355631/1‑110 (MQ=255)
                                                                                   aTGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCaa                                                                                                    <  1:355631/110‑1 (MQ=255)
                                                                                       tGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctgg                                                        <  2:1139492/150‑1 (MQ=255)
                                                                                        ggAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgc                                                                                                        <  2:937968/101‑1 (MQ=255)
                                                                                        ggAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgc                                                                                                        >  1:937968/1‑101 (MQ=255)
                                                                                        ggAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtg                                                                                             >  2:748816/1‑112 (MQ=255)
                                                                                        ggAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtg                                                                                             <  1:748816/112‑1 (MQ=255)
                                                                                         gAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctggct                                                      >  1:912420/1‑150 (MQ=255)
                                                                                         gAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTAATGCCAGCgctggct                                                      >  4:633469/1‑150 (MQ=255)
                                                                                           aGATGGTCACTTTTTGTGCGATCAGGCAATGGGTTTCTGTGTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCtgt                                                    <  2:211197/150‑1 (MQ=255)
                                                                                                gTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccgc                                               >  2:334350/1‑150 (MQ=255)
                                                                                                  cACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGccgccg                                             <  2:291461/150‑1 (MQ=255)
                                                                                                       tttGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgcg                                        >  3:575398/1‑150 (MQ=255)
                                                                                                        ttGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgcgc                                       <  3:252277/150‑1 (MQ=255)
                                                                                                           cgcgATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCaaa                                    >  1:169171/1‑150 (MQ=255)
                                                                                                           cgcgATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCaaa                                    >  3:153116/1‑150 (MQ=255)
                                                                                                           cgcgATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCaaa                                    >  3:68217/1‑150 (MQ=255)
                                                                                                           cgcgATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCaaa                                    >  3:67331/1‑150 (MQ=255)
                                                                                                            gcgATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAAc                                   >  4:516322/1‑150 (MQ=255)
                                                                                                               aTCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAAcgcg                                >  1:820977/1‑150 (MQ=255)
                                                                                                                  aGGCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATg                             <  2:130671/150‑1 (MQ=255)
                                                                                                                    gCAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATggg                           <  2:651041/150‑1 (MQ=255)
                                                                                                                     cAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATgggg                          <  2:504236/150‑1 (MQ=255)
                                                                                                                     cAATGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATgggg                          <  2:542995/150‑1 (MQ=255)
                                                                                                                      aaTGGGTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGgt                         >  3:397383/1‑150 (MQ=255)
                                                                                                                          ggTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGgttgtt                     >  2:924231/1‑150 (MQ=255)
                                                                                                                           gTTTCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGttt                    <  1:626346/150‑1 (MQ=255)
                                                                                                                           gTTTCTGTTTTGAAGCCGGGGAGGAGGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGttt                    <  2:211270/150‑1 (MQ=255)
                                                                                                                             ttCTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTAt                  >  2:225415/1‑150 (MQ=255)
                                                                                                                               cTGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCt                <  1:519061/150‑1 (MQ=255)
                                                                                                                                tGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTg               >  1:335774/1‑150 (MQ=255)
                                                                                                                                tGTTTTGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTg               <  2:769774/150‑1 (MQ=255)
                                                                                                                                  ttttGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGcc             >  4:762547/1‑150 (MQ=255)
                                                                                                                                  ttttGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGcc             <  1:932757/150‑1 (MQ=255)
                                                                                                                                    ttGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGt           >  3:330563/1‑150 (MQ=255)
                                                                                                                                    ttGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGt           >  1:392115/1‑150 (MQ=255)
                                                                                                                                     tGAAGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTg          <  2:854816/150‑1 (MQ=255)
                                                                                                                                       aaGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCa        >  1:712517/1‑150 (MQ=255)
                                                                                                                                        aGCCGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCAt       >  4:50910/1‑150 (MQ=255)
                                                                                                                                           cGGGGAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTaa    <  2:478905/150‑1 (MQ=255)
                                                                                                                                            ggggAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAg   >  2:754935/1‑150 (MQ=255)
                                                                                                                                            ggggAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAGCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAg   >  4:169815/1‑150 (MQ=255)
                                                                                                                                             gggAGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGc  >  1:1035964/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
TGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGC  >  NC_000913_3_pae_pgi/4158983‑4159273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: