Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 1,166,727 G→C E215Q (GAA→CAA)  ndh → respiratory NADH dehydrogenase 2/cupric reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,166,7270GC99.0% 364.8 / NA 104E215Q (GAA→CAA) ndhrespiratory NADH dehydrogenase 2/cupric reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (49/54);  minor base T (0/1);  total (49/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

GCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGAA  >  NC_000913_3_pae_pgi/1166583‑1166873
                                                                                                                                                |                                                                                                                                                  
gcgAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCg                                                                                                                                               >  2:199150/1‑150 (MQ=255)
  gAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTACGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCggg                                                                                                                                             >  2:115657/1‑150 (MQ=255)
    aTGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGgag                                                                                                                                           <  1:541307/150‑1 (MQ=255)
      ggCAAAGTGAACATGGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGaa                                                                                                                                         <  1:199150/150‑1 (MQ=255)
       gCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAAc                                                                                                                                        >  4:154657/1‑150 (MQ=255)
       gCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAAc                                                                                                                                        >  1:366013/1‑150 (MQ=255)
        cAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGACTTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACg                                                                                                                                       >  2:650260/1‑150 (MQ=255)
             tGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTAttt                                                                                                                                  <  1:405358/150‑1 (MQ=255)
              gAACATTGCGATTGTCGGCGTCGGCGCGACGGGTGTAGACCTCTACGCTGAATTTCAAAACGCAGTCAAACAACTGCACAGCAAAGGTTACAAAGGCCTGACCAATGAAGACATGAACGTAACGCTGGTACAATCGGGAGAACGTAtttt                                                                                                                                 <  2:784193/150‑1 (MQ=255)
              gAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTAtttt                                                                                                                                 <  2:632824/150‑1 (MQ=255)
                  aTTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCt                                                                                                                             >  3:495115/1‑150 (MQ=255)
                    tGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGc                                                                                                                           <  2:215053/150‑1 (MQ=255)
                    tGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGc                                                                                                                           <  3:239490/150‑1 (MQ=255)
                     gCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGc                                                                                                                                                <  1:442905/128‑1 (MQ=255)
                     gCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGc                                                                                                                                                >  2:442905/1‑128 (MQ=255)
                      cGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGt                                                                                                                         <  2:937381/150‑1 (MQ=255)
                           gTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCCGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCg                                                                                                                    >  3:484033/1‑150 (MQ=255)
                            tCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGc                                                                                                                   >  1:353980/1‑150 (MQ=255)
                            tCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGc                                                                                                                   <  4:59279/150‑1 (MQ=255)
                               gcggcggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCAc                                                                                                                >  3:726666/1‑150 (MQ=255)
                               gcggcggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCAc                                                                                                                >  3:411309/1‑150 (MQ=255)
                               gcggcggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCAc                                                                                                                >  3:136698/1‑150 (MQ=255)
                                     gagcggcgGGTGTAGAACTCTCCGCTGCATGGCACAACGCGGTCAAGCAACTGCCCAGCTACGGTTACAAAGGCCTGACCACGGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTAtct                                                                                                          <  3:422794/148‑1 (MQ=255)
                                         gACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTctgc                                                                                                      <  1:535965/150‑1 (MQ=255)
                                           cGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTctgctg                                                                                                    <  3:154657/150‑1 (MQ=255)
                                             ggTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCg                                                                                                  <  2:1148163/150‑1 (MQ=255)
                                               tgtAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAGGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGc                                                                                                >  1:954033/1‑150 (MQ=255)
                                               tgtAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGc                                                                                                >  1:953845/1‑150 (MQ=255)
                                                gtAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGcc                                                                                               <  4:345419/150‑1 (MQ=255)
                                                 tAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGccc                                                                                              <  4:526812/150‑1 (MQ=255)
                                                 tAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGccc                                                                                              <  4:526483/150‑1 (MQ=255)
                                                  aGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCca                                                                                             >  1:522125/1‑150 (MQ=255)
                                                      ctctCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAAc                                                                                         <  4:136698/150‑1 (MQ=255)
                                                        ctcCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGa                                                                                       <  2:282272/150‑1 (MQ=255)
                                                         tcCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAg                                                                                      >  2:744956/1‑150 (MQ=255)
                                                           cGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCt                                                                                    >  2:1008826/1‑150 (MQ=255)
                                                                 aTTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTAtct                                                                                                          <  1:505532/122‑1 (MQ=255)
                                                                 aTTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTAtct                                                                                                          >  2:505532/1‑122 (MQ=255)
                                                                  ttGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGaaa                                                                             >  4:704645/1‑150 (MQ=255)
                                                                    gCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCTCACAACGAGCTAACGAAACt                                                                           >  1:933537/1‑150 (MQ=255)
                                                                      acaACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCgg                                                                                                                                              >  2:128279/1‑81 (MQ=255)
                                                                      acaACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCgg                                                                                                                                              <  1:128279/81‑1 (MQ=255)
                                                                      acaACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTg                                                                         <  3:37869/150‑1 (MQ=255)
                                                                        caCGCGGTCAAGCAACTGCACAGCTAAGGTTCCAACGGCCTGACCAACGAAGCACTGAACGTCACGCTGGTACCCGCGGCAGAACGTATTTTGCCTGCGTTCCCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGc                                                                       <  2:308109/149‑1 (MQ=255)
                                                                        aaCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGc                                                                       >  4:729313/1‑150 (MQ=255)
                                                                        aaCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGc                                                                       <  1:1008826/150‑1 (MQ=255)
                                                                        aaCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGc                                                                       <  2:366013/150‑1 (MQ=255)
                                                                         aCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCg                                                                      >  3:453435/1‑150 (MQ=255)
                                                                          cgcgGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGt                                                                     >  4:184175/1‑150 (MQ=255)
                                                                           gcgGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGtt                                                                    >  3:396683/1‑150 (MQ=255)
                                                                            cgGTCNAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCNCNTNTCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTc                                                                   <  1:115657/150‑1 (MQ=255)
                                                                             ggTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTcg                                                                  >  3:281071/1‑150 (MQ=255)
                                                                              gTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTcgc                                                                 <  1:744956/150‑1 (MQ=255)
                                                                               tCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTcgcg                                                                >  1:933541/1‑150 (MQ=255)
                                                                                cAAGCAGCTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGt                                                               <  2:519048/150‑1 (MQ=255)
                                                                                cAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGt                                                               <  4:484033/150‑1 (MQ=255)
                                                                                  aGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGc                                                             <  1:639091/150‑1 (MQ=255)
                                                                                   gCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGt                                                                                                              <  2:947787/100‑1 (MQ=255)
                                                                                   gCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGt                                                                                                              >  1:947787/1‑100 (MQ=255)
                                                                                   gCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCt                                                            <  3:705125/150‑1 (MQ=255)
                                                                                    cAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTg                                                           >  4:538499/1‑150 (MQ=255)
                                                                                     aaCTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGa                                                          <  2:269340/150‑1 (MQ=255)
                                                                                      aCTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGAc                                                         <  3:390390/150‑1 (MQ=255)
                                                                                        tGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCTCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGc                                                       <  2:933537/150‑1 (MQ=255)
                                                                                          cacaGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCaa                                                     >  3:772583/1‑150 (MQ=255)
                                                                                          cacaGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCaa                                                     <  4:453435/150‑1 (MQ=255)
                                                                                             aGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAAcc                                                  <  2:1018735/150‑1 (MQ=255)
                                                                                                 aCGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATgg                                              <  3:206320/150‑1 (MQ=255)
                                                                                                  cGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGt                                             <  3:316324/150‑1 (MQ=255)
                                                                                                     ttACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCAc                                          <  1:905969/150‑1 (MQ=255)
                                                                                                       aCAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCa                                        <  4:166825/150‑1 (MQ=255)
                                                                                                       aCAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCa                                        <  4:495115/150‑1 (MQ=255)
                                                                                                         aaaGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGt                                      >  4:494365/1‑150 (MQ=255)
                                                                                                           aGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACaa                                                                                          >  2:1157785/1‑96 (MQ=255)
                                                                                                           aGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACaa                                                                                          <  1:1157785/96‑1 (MQ=255)
                                                                                                           aGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGc                                    <  2:134008/150‑1 (MQ=255)
                                                                                                           aGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGc                                    <  2:353980/150‑1 (MQ=255)
                                                                                                             gCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCtg                                  >  1:913915/1‑150 (MQ=255)
                                                                                                               cTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCtgat                                <  2:1043057/150‑1 (MQ=255)
                                                                                                               cTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCtgat                                <  4:726666/150‑1 (MQ=255)
                                                                                                                 gACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCtgatga                              <  1:689952/150‑1 (MQ=255)
                                                                                                                  aCCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGaa                             >  3:482861/1‑150 (MQ=255)
                                                                                                                   ccAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAg                            <  3:494365/150‑1 (MQ=255)
                                                                                                                     aaCGAAGCGCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAggc                          >  2:239968/1‑150 (MQ=255)
                                                                                                                      aCGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAggcg                         >  1:1165678/1‑150 (MQ=255)
                                                                                                                        gAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAggcggc                       >  4:245571/1‑150 (MQ=255)
                                                                                                                           gCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACTAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTg                    <  1:716511/150‑1 (MQ=255)
                                                                                                                             ccTGAACGTAACGCTGGTACAAGCGGGGGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGca                  >  2:880048/1‑150 (MQ=255)
                                                                                                                             ccTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGca                  >  3:464970/1‑150 (MQ=255)
                                                                                                                              cTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGcac                 >  4:706094/1‑150 (MQ=255)
                                                                                                                              cTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGcac                 >  1:397172/1‑150 (MQ=255)
                                                                                                                                   cGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTaa            >  3:290150/1‑150 (MQ=255)
                                                                                                                                    gTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTaaa           <  1:276807/150‑1 (MQ=255)
                                                                                                                                       aCGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGAt        <  3:704645/150‑1 (MQ=255)
                                                                                                                                       aCGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGAt        >  1:1202813/1‑150 (MQ=255)
                                                                                                                                         gCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCaa                                                     <  4:691350/103‑1 (MQ=255)
                                                                                                                                         gCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCaa                                                     >  3:691350/1‑103 (MQ=255)
                                                                                                                                           tGGTACAAGCGGGAGAACGTATTATGCCTGCGAAACGGCTGCGGATCTCGGCGGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCg    <  3:729313/150‑1 (MQ=255)
                                                                                                                                             gTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGc     >  3:51536/1‑147 (MQ=255)
                                                                                                                                             gTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGaa  >  2:630160/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGAA  >  NC_000913_3_pae_pgi/1166583‑1166873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: