Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 2,416,374 T→G M543R (ATG→AGG)  pta → phosphate acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi2,416,3740TG99.5% 677.6 / ‑4.2 188M543R (ATG→AGG) ptaphosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (99/88);  total (100/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 196 total aligned reads displayed.

TGGTGCTCGGTACGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCATGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTAC  >  NC_000913_3_pae_pgi/2416227‑2416519
                                                                                                                                                   |                                                                                                                                                 
    gCTCGGTACGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCGAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAggctgct                                                                                                                                             >  2:1208842/1‑150 (MQ=255)
    gCTCGGTACGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAggctgct                                                                                                                                             <  4:604268/150‑1 (MQ=255)
     cTCGGTACGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAggctg                                                                                                                                               <  2:1557248/147‑1 (MQ=255)
     cTCGGTACGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAggctg                                                                                                                                               >  1:1557248/1‑147 (MQ=255)
           aCGCTGATGCTGGAACAGGATGAAGTTCATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGaa                                                                                                                                      >  2:1893780/1‑150 (MQ=255)
                gATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGgt                                                                                                                                 >  1:1921242/1‑150 (MQ=255)
                    cTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGgtttac                                                                                                                             <  1:1090973/150‑1 (MQ=255)
                     tGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAggctgct                                                                                                                                             <  3:956938/133‑1 (MQ=255)
                       gAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGgtttacgtt                                                                                                                          >  2:236380/1‑150 (MQ=255)
                          cAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGgtttacgtttac                                                                                                                       <  2:748641/150‑1 (MQ=255)
                           aGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGtttacgtttacg                                                                                                                      <  4:396077/150‑1 (MQ=255)
                               tGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGt                                                                                                                    >  1:1776015/1‑148 (MQ=255)
                                gAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGAc                                                                                                                 >  2:124337/1‑150 (MQ=255)
                                     tGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGc                                                                                                            >  3:649814/1‑150 (MQ=255)
                                       aTGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGa                                                                                                          >  1:1577838/1‑150 (MQ=255)
                                       aTGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGa                                                                                                          <  3:336974/150‑1 (MQ=255)
                                         ggTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATc                                                                                                        >  2:1507602/1‑150 (MQ=255)
                                         ggTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATc                                                                                                        <  2:40794/150‑1 (MQ=255)
                                           tCTGGTTTCCGGCGATGTTAACTCACCCGCAAACACCATCGGGCCGCCGCTTCAGCCGATCAAAACTGCACCGGGCAGCGCCCTGGTATCTTCCGTGTGCTTCAGGCTGCTGCCGGAACAGGGTTACGTTTACGGTGACTGCGCGATCaa                                                                                                      >  4:616101/1‑150 (MQ=255)
                                             tGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGTTATCTTCCGTGTTCTTCAGGCTGCTGCCTGAACAGGTTTACGTTTACGGTGACTGTGCGATCAAcc                                                                                                    <  3:489785/150‑1 (MQ=255)
                                               gTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCg                                                                                                  >  3:48279/1‑150 (MQ=255)
                                               gTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCg                                                                                                  >  1:7929/1‑150 (MQ=255)
                                                tttCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCgg                                                                                                 <  1:483544/150‑1 (MQ=255)
                                                    cGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGTTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATcc                                                                                             >  3:673165/1‑150 (MQ=255)
                                                     ggTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACtgt                                                                                                              <  1:1005847/132‑1 (MQ=255)
                                                     ggTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCg                                                                                            >  1:1413870/1‑150 (MQ=255)
                                                     ggTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCg                                                                                            >  1:674961/1‑150 (MQ=255)
                                                       tGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGAc                                                                                          <  1:599098/150‑1 (MQ=255)
                                                            ttCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAAccc                                                                                                   >  1:1007729/1‑136 (MQ=255)
                                                            ttCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAAccc                                                                                                   <  2:1007729/136‑1 (MQ=255)
                                                            ttCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTg                                                                                     >  1:729695/1‑150 (MQ=255)
                                                            ttCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTg                                                                                     >  4:144881/1‑150 (MQ=255)
                                                             tCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGa                                                                                    >  1:433853/1‑150 (MQ=255)
                                                              cacaCTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGCTCAATTCTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGaa                                                                                   <  1:1501477/150‑1 (MQ=255)
                                                              cacaCTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGgtttacgttt                                                                                                                         <  1:1307279/112‑1 (MQ=255)
                                                              cacaCTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGaa                                                                                   <  3:1113738/150‑1 (MQ=255)
                                                              cacaCTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGaa                                                                                   >  3:754120/1‑150 (MQ=255)
                                                               tcacTATCGCAATCACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAAc                                                                                  <  2:873617/149‑1 (MQ=255)
                                                                 acTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAg                                                                                <  2:1715129/150‑1 (MQ=255)
                                                                 acTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCAACGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAg                                                                                >  2:1306662/1‑150 (MQ=255)
                                                                   tACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCt                                                                              >  1:1739868/1‑150 (MQ=255)
                                                                     ccGCAAACACCATCCGTCCGCCGCTGCCGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCGGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTgg                                                                            >  2:179299/1‑150 (MQ=255)
                                                                     ccGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTgg                                                                            <  2:1284501/150‑1 (MQ=255)
                                                                     ccGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTgg                                                                            >  2:468265/1‑150 (MQ=255)
                                                                      cGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGt                                                                                                                    >  4:539578/1‑109 (MQ=255)
                                                                      cGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGc                                                                           >  2:374804/1‑150 (MQ=255)
                                                                      cGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGc                                                                           <  1:1322273/150‑1 (MQ=255)
                                                                        cAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAAccc                                                                                                   >  1:36182/1‑124 (MQ=255)
                                                                        cAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAg                                                                         >  1:372492/1‑150 (MQ=255)
                                                                        cAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAg                                                                         >  1:160305/1‑150 (MQ=255)
                                                                        cAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAg                                                                         >  4:698740/1‑150 (MQ=255)
                                                                         aaaCACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGa                                                                        >  3:530199/1‑150 (MQ=255)
                                                                              ccATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATcg                                                                   <  2:1921242/150‑1 (MQ=255)
                                                                              ccATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATcg                                                                   <  2:110109/150‑1 (MQ=255)
                                                                               cATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATcgc                                                                  <  2:1047873/150‑1 (MQ=255)
                                                                                aTCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACtgt                                                                                                              >  1:992282/1‑105 (MQ=255)
                                                                                aTCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGc                                                                                       >  2:1742334/1‑128 (MQ=255)
                                                                                aTCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGc                                                                                       <  1:1742334/128‑1 (MQ=255)
                                                                                 tccgtccgCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGa                                                                <  4:48279/150‑1 (MQ=255)
                                                                                 tccgtccgCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGa                                                                <  2:796315/150‑1 (MQ=255)
                                                                                   cgtccgCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGAtt                                                              <  1:124337/150‑1 (MQ=255)
                                                                                    gtccgCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTc                                                             <  4:1105082/150‑1 (MQ=255)
                                                                                     tccgCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCa                                                            <  2:347330/150‑1 (MQ=255)
                                                                                        gccgcTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTc                                                         >  4:625719/1‑150 (MQ=255)
                                                                                            cTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCt                                                     <  3:651668/150‑1 (MQ=255)
                                                                                                  cTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTcc                                               <  2:1413870/150‑1 (MQ=255)
                                                                                                      tCAAAACTGCACCGGGCAGCTACCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTg                                           <  1:1963703/150‑1 (MQ=255)
                                                                                                       cAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGc                                          >  4:1233459/1‑150 (MQ=255)
                                                                                                        aaaaCTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCg                                         >  3:56854/1‑150 (MQ=255)
                                                                                                         aaaCTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCgg                                        >  4:677058/1‑150 (MQ=255)
                                                                                                          aaCTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGc                                       <  4:835694/150‑1 (MQ=255)
                                                                                                             tGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCtt                                    >  2:1829846/1‑150 (MQ=255)
                                                                                                             tGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCtt                                    >  2:1647578/1‑150 (MQ=255)
                                                                                                               cACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCg                                  <  1:1208842/150‑1 (MQ=255)
                                                                                                                aCCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCgg                                 <  1:1647578/150‑1 (MQ=255)
                                                                                                                 ccGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGt                                <  4:660017/150‑1 (MQ=255)
                                                                                                                  cGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTa                               >  2:1577434/1‑150 (MQ=255)
                                                                                                                   gggCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTAt                              <  2:847189/150‑1 (MQ=255)
                                                                                                                   gggCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTAt                              <  2:7929/150‑1 (MQ=255)
                                                                                                                     gCAGCTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCg                            <  1:385948/150‑1 (MQ=255)
                                                                                                                         cTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCgg                                 >  4:215048/1‑141 (MQ=255)
                                                                                                                         cTCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCgg                                 <  3:215048/141‑1 (MQ=255)
                                                                                                                          tCCCTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACcg                       >  2:929810/1‑150 (MQ=255)
                                                                                                                            ccTGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTATGGTGACTGTGCGATCAACCCGGATCCGa                                                                                           >  2:1314961/1‑80 (MQ=255)
                                                                                                                            ccTGGTATCTTCCGTGTTCTTCAGGCTGCTACCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCg                                              >  1:1815576/1‑125 (MQ=255)
                                                                                                                            ccTGGTATCTTCCGTGTTCTTCAGGCTGCTACCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCg                                              <  2:1815576/125‑1 (MQ=255)
                                                                                                                            ccTGGTATCTTCCGTGTTCTTCAGGCTGATGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACcgcg                     >  3:1227343/1‑150 (MQ=255)
                                                                                                                              tGGTATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACcgcgcg                   >  3:360362/1‑150 (MQ=255)
                                                                                                                                 tATCTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTg                <  1:1893523/150‑1 (MQ=255)
                                                                                                                                    cTTCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTa             <  1:1834552/150‑1 (MQ=255)
                                                                                                                                     ttCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCt                                                     >  3:621863/1‑109 (MQ=255)
                                                                                                                                     ttCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTAt            >  3:1123287/1‑150 (MQ=255)
                                                                                                                                      tCCGTGTTCTTGAGGCTGCTGCCGGAAAAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATAGCGATTCAGTCCGCTGATTCCGCTGCGCCCTTCGGAAACGAAACGAGAGTTGCCATg           >  2:1627942/1‑150 (MQ=255)
                                                                                                                                      tCCGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCt                                                                                      >  1:494732/1‑75 (MQ=255)
                                                                                                                                        catgtTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGct         >  2:1936078/3‑150 (MQ=255)
                                                                                                                                        cGTGTTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGct         <  4:90491/150‑1 (MQ=255)
                                                                                                                                           gtTCTTCAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCTGTATCGAACCGCGCGTTGCTATGCtctc      <  2:69876/150‑1 (MQ=255)
                                                                                                                                            ttcttcAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTcc     <  1:806142/150‑1 (MQ=255)
                                                                                                                                               ttcAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTAc  >  3:147394/1‑150 (MQ=255)
                                                                                                                                               ttcAGGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTAc  >  1:63176/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGGTGCTCGGTACGCTGATGCTGGAACAGGATGAAGTTGATGGTCTGGTTTCCGGTGCTGTTCACACTACCGCAAACACCATCCGTCCGCCGCTGCAGCTGATCAAAACTGCACCGGGCAGCTCCCTGGTATCTTCCGTGTTCTTCATGCTGCTGCCGGAACAGGTTTACGTTTACGGTGACTGTGCGATCAACCCGGATCCGACCGCTGAACAGCTGGCAGAAATCGCGATTCAGTCCGCTGATTCCGCTGCGGCCTTCGGTATCGAACCGCGCGTTGCTATGCTCTCCTAC  >  NC_000913_3_pae_pgi/2416227‑2416519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: