Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 3,316,818 C→G A237P (GCG→CCG)  nusA ← transcription termination/antitermination L factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi3,316,8180CG98.5% 450.0 / ‑6.5 130A237P (GCG→CCG) nusAtranscription termination/antitermination L factor
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base G (65/62);  total (66/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 130 total aligned reads displayed.

TAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCG  >  NC_000913_3_pae_pgi/3316671‑3316964
                                                                                                                                                   |                                                                                                                                                  
tAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGa                                                                                                                                                  >  1:149426/1‑150 (MQ=255)
    cACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGAtttt                                                                                                                                              >  2:194963/1‑150 (MQ=255)
               ccGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAAc                                                                                                                                   <  1:826663/150‑1 (MQ=255)
               ccGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAAc                                                                                                                                   >  2:739566/1‑150 (MQ=255)
                  ggTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCg                                                                                                                                <  3:212960/150‑1 (MQ=255)
                  ggTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGACAACCCg                                                                                                                                >  4:353034/1‑150 (MQ=255)
                         atcCCACAGGACGATATCGATACGCGCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATcgcg                                                                                                                         <  4:728441/150‑1 (MQ=255)
                          tcCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGa                                                                                                                        <  3:678197/150‑1 (MQ=255)
                           cccACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCa                                                                                                                                          <  1:1170914/131‑1 (MQ=255)
                           cccACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCa                                                                                                                                          >  2:1170914/1‑131 (MQ=255)
                            ccACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGc                                                                                                                      >  1:21109/1‑150 (MQ=255)
                                aGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTCGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCgct                                                                                                                  <  3:499752/150‑1 (MQ=255)
                                 ggACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCgctg                                                                                                                 <  3:122033/150‑1 (MQ=255)
                                  gACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGCTTATCGTTGGCTTTCACCGGGATTTTCGCCCGAGAACCCGGATCGCGAGCCgctgc                                                                                                                >  2:1195757/1‑150 (MQ=255)
                                    cGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCtt                                                                                                              >  1:249342/1‑150 (MQ=255)
                                    cGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCtt                                                                                                              >  3:434591/1‑150 (MQ=255)
                                     gATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCgctgc                                                                                                                <  1:330651/147‑1 (MQ=255)
                                     gATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCgctgc                                                                                                                >  2:330651/1‑147 (MQ=255)
                                     gATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCttt                                                                                                             >  1:1008229/1‑150 (MQ=255)
                                     gATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCttt                                                                                                             >  3:671752/1‑150 (MQ=255)
                                      atatCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTa                                                                                                            <  1:540953/150‑1 (MQ=255)
                                       tatCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGATAACCCGGATCGCGAGCCGCTGCTTTaa                                                                                                           <  4:621951/150‑1 (MQ=255)
                                         tCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAAtt                                                                                                         >  3:706457/1‑150 (MQ=255)
                                           gATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTc                                                                                                       >  4:559759/1‑150 (MQ=255)
                                           gATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTc                                                                                                       >  2:429175/1‑150 (MQ=255)
                                           gATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTc                                                                                                       >  1:1109594/1‑150 (MQ=255)
                                           gATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTc                                                                                                       <  3:334391/150‑1 (MQ=255)
                                           gATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCGGCTTTAATTTc                                                                                                       >  1:755664/1‑150 (MQ=255)
                                              aCGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAAt                                                                                                    <  1:630777/150‑1 (MQ=255)
                                                gCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAAcc                                                                                                                                  >  4:329219/1‑118 (MQ=255)
                                                gCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCa                                                                                                  >  1:91472/1‑150 (MQ=255)
                                                gCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCa                                                                                                  >  2:841674/1‑150 (MQ=255)
                                                   cGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCActt                                                                                               <  4:161450/150‑1 (MQ=255)
                                                       aCCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACttcttc                                                                                           <  4:706457/150‑1 (MQ=255)
                                                        cccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCg                                                                                          <  1:945764/150‑1 (MQ=255)
                                                        cccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTCTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCg                                                                                          >  2:264715/1‑150 (MQ=255)
                                                         ccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCActtc                                                                                              >  1:281365/1‑145 (MQ=255)
                                                         ccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCActtc                                                                                              <  2:281365/145‑1 (MQ=255)
                                                             ttCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGAt                                                                                     <  1:739566/150‑1 (MQ=255)
                                                                 gTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCt                                                                                 <  2:176313/150‑1 (MQ=255)
                                                                  tAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTg                                                                                <  4:671752/150‑1 (MQ=255)
                                                                   aGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTgg                                                                               <  3:106833/150‑1 (MQ=255)
                                                                   aGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTgg                                                                               <  1:841674/150‑1 (MQ=255)
                                                                    gACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGc                                                                              <  2:676488/150‑1 (MQ=255)
                                                                     acacCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCa                                                                             <  3:252558/150‑1 (MQ=255)
                                                                         cGCCTGAACACGCGCGCCACGCGTACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTc                                                                         <  1:1149379/150‑1 (MQ=255)
                                                                          gCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCa                                                                        >  1:1005053/1‑150 (MQ=255)
                                                                             tGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATa                                                                     <  4:262741/150‑1 (MQ=255)
                                                                             tGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATa                                                                     >  2:1021868/1‑150 (MQ=255)
                                                                              gAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATAc                                                                    <  2:149426/150‑1 (MQ=255)
                                                                                  acGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGCTTTCTGGCACTTCAATACGGaa                                                                >  2:855829/1‑150 (MQ=255)
                                                                                  acGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGaa                                                                >  1:598706/1‑150 (MQ=255)
                                                                                       cgcCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGtt                                                           <  2:1008229/150‑1 (MQ=255)
                                                                                        gcCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTc                                                          >  4:416081/1‑150 (MQ=255)
                                                                                         ccACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCg                                                         <  2:477996/150‑1 (MQ=255)
                                                                                            cGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATc                                                      <  3:486551/150‑1 (MQ=255)
                                                                                             gCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCa                                                     >  4:639779/1‑150 (MQ=255)
                                                                                               aTACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGCGTTTCGCACGCGAACCCGGCTCGCGAGCCGCCGCTTTAATTTCAATCACTTCTTCGCGGATTTCTGGCACCGCAATACGGAACAGTTCGATCCGc                                                   >  2:929483/1‑150 (MQ=255)
                                                                                               aTACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGc                                                   <  4:434591/150‑1 (MQ=255)
                                                                                                   cTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCAttt                                               >  3:728654/1‑150 (MQ=255)
                                                                                                       gCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCgg                                           <  2:156340/150‑1 (MQ=255)
                                                                                                         aaGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTaa                                                                                                           >  1:647901/1‑84 (MQ=255)
                                                                                                         aaGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTaa                                                                                                           <  2:647901/84‑1 (MQ=255)
                                                                                                         aaGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTgg                                                                               >  2:534084/1‑112 (MQ=255)
                                                                                                         aaGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTgg                                                                               <  1:534084/112‑1 (MQ=255)
                                                                                                           gCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTg                                       <  1:429175/150‑1 (MQ=255)
                                                                                                               cTACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAAc                                   <  2:1109779/150‑1 (MQ=255)
                                                                                                                tACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGCACAGTTCGATCAGCATTTCCGGCTTGGAACg                                  >  2:568112/1‑150 (MQ=255)
                                                                                                                tACCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACg                                  >  1:1111967/1‑150 (MQ=255)
                                                                                                                 aCCGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGa                                 <  2:231854/150‑1 (MQ=255)
                                                                                                                  ccGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCgctgc                                                                                                                <  2:832454/70‑1 (MQ=255)
                                                                                                                  ccGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCgctgc                                                                                                                >  1:832454/1‑70 (MQ=255)
                                                                                                                  ccGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGcc                                                                                        <  1:1023410/94‑1 (MQ=255)
                                                                                                                  ccGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGcc                                                                                        >  2:1023410/1‑94 (MQ=255)
                                                                                                                  ccGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAg                                <  2:249342/150‑1 (MQ=255)
                                                                                                                   cGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGGGt                               >  4:202276/1‑150 (MQ=255)
                                                                                                                   cGGATCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGt                               >  2:904195/1‑150 (MQ=255)
                                                                                                                      aTCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGAc                            <  2:598706/150‑1 (MQ=255)
                                                                                                                      aTCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGAc                            >  2:1099944/1‑150 (MQ=255)
                                                                                                                      aTCGATACGTTGATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGTTCGCGAGCCGCTGCTTTAAATTCAATCACTTCTACGCCGATTTCAGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGAc                            <  1:428068/150‑1 (MQ=255)
                                                                                                                       tCGATACGTTTATCGTTGGTTTTCACCGTGATTTTCGCACGGGAGCCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTCCTTCGCCGATTTGTGGCACTTCAATACGGAACAGTTCGAGCAGCATTGCCGGCTTGGTACGAGTGACg                           <  4:390324/150‑1 (MQ=255)
                                                                                                                       tCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACg                           <  1:918298/150‑1 (MQ=255)
                                                                                                                       tCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACg                           >  3:288304/1‑150 (MQ=255)
                                                                                                                           tACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGCAACGAGTGACGAACa                       >  2:422158/1‑150 (MQ=255)
                                                                                                                            aCGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAg                      <  2:1109594/150‑1 (MQ=255)
                                                                                                                                 tATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGc                                                                                         >  3:428033/1‑78 (MQ=255)
                                                                                                                                 tATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGc                                                                                         <  4:428033/78‑1 (MQ=255)
                                                                                                                                 tATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTgcg                 >  2:486862/1‑150 (MQ=255)
                                                                                                                                  aTCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCATTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGATCAGTTgcgc                <  3:590055/150‑1 (MQ=255)
                                                                                                                                     gTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGcc             >  2:292056/1‑150 (MQ=255)
                                                                                                                                      ttGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCa            >  1:288232/1‑150 (MQ=255)
                                                                                                                                      ttGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCa            >  1:288105/1‑150 (MQ=255)
                                                                                                                                         gTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCAcgc         >  3:493260/1‑150 (MQ=255)
                                                                                                                                           tttCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGtt                                                           <  1:1003041/98‑1 (MQ=255)
                                                                                                                                           tttCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGtt                                                           >  2:1003041/1‑98 (MQ=255)
                                                                                                                                            ttCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAAt                                                                                                    >  2:1160988/1‑56 (MQ=255)
                                                                                                                                             tCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGCTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCtt     <  3:639779/150‑1 (MQ=255)
                                                                                                                                              cACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTc    <  3:559759/150‑1 (MQ=255)
                                                                                                                                              cACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTc    <  3:353034/150‑1 (MQ=255)
                                                                                                                                                ccGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCg  >  3:696392/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCG  >  NC_000913_3_pae_pgi/3316671‑3316964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: