Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_pgi	1,166,727	G→C	E215Q (GAA→CAA)	ndh →	respiratory NADH dehydrogenase 2/cupric reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_pgi	1,166,727	0	G	C	100.0%	307.9 / NA	87	E215Q (GAA→CAA)	ndh	respiratory NADH dehydrogenase 2/cupric reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (38/49);  total (38/49)

GCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGAA  >  NC_000913_3_pae_pgi/1166581‑1166873                                                                                                                                                   |                                                                                                                                                   gcgcGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAg                                                                                                                                                 >  4:34323/1‑150 (MQ=255)   gcgAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGcc                                                                                                                                               >  4:50755/1‑149 (MQ=255)    cgAATGGCAAAGGCAACATTGCGATTGTCGGCGGCGGCGCGGCGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGCCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCgg                                                                                                                                              <  4:332210/150‑1 (MQ=255)     gAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCggg                                                                                                                                             <  4:421065/150‑1 (MQ=255)     gAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCggg                                                                                                                                             >  4:641544/1‑150 (MQ=255)         ggCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGaa                                                                                                                                         >  2:1216374/1‑150 (MQ=255)          gCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAAc                                                                                                                                        >  1:335021/1‑150 (MQ=255)          gCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAAc                                                                                                                                        <  4:300521/150‑1 (MQ=255)                 gAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTAtttt                                                                                                                                 <  1:961326/150‑1 (MQ=255)                   aCATTGCGATTGTCGGCGGCGGCGCGACGCGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGc                                                                                                                               <  1:269689/150‑1 (MQ=255)                         cGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGt                                                                                                                         <  4:328714/150‑1 (MQ=255)                         cGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGt                                                                                                                         <  1:94324/150‑1 (MQ=255)                            ttGTCGGCGGCGGCGCGACGGGTGTAGACCTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTAc                                                                                                                      <  2:386891/150‑1 (MQ=255)                              gTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCg                                                                                                                    <  2:485970/150‑1 (MQ=255)                               tCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGc                                                                                                                   <  2:293404/150‑1 (MQ=255)                                cggcggcggCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACAGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGcc                                                                                                                  <  3:537683/150‑1 (MQ=255)                                 ggcggcggcGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCa                                                                                                                 >  4:568696/1‑150 (MQ=255)                                  gcggcggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCAc                                                                                                                <  2:166914/150‑1 (MQ=255)                                    ggcggcgCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGt                                                                                                              <  2:38081/150‑1 (MQ=255)                                          gcgACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATctct                                                                                                        <  2:1133650/150‑1 (MQ=255)                                           cgACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTctg                                                                                                       <  3:237407/150‑1 (MQ=255)                                            gACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTctgc                                                                                                      <  3:509927/150‑1 (MQ=255)                                            gACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTctgc                                                                                                      <  4:754702/150‑1 (MQ=255)                                             aCGGGTGTAGATCTCGCTGGTGGCTGGCACATCAAGAACAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTctgct                                                                                                     >  2:940969/1‑150 (MQ=255)                                             aCGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTctgct                                                                                                     <  2:179051/150‑1 (MQ=255)                                                 gtgtAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCgg                                                                                                 <  1:476494/150‑1 (MQ=255)                                                   gtAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCGCAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGcc                                                                                               >  4:72533/1‑150 (MQ=255)                                                   gtAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGcc                                                                                               <  1:943073/150‑1 (MQ=255)                                                     aGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCca                                                                                             <  3:410194/150‑1 (MQ=255)                                                      gAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCcac                                                                                            >  2:943147/1‑150 (MQ=255)                                                        aCTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACaa                                                                                          <  2:1111372/150‑1 (MQ=255)                                                         ctctCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAAc                                                                                         <  2:379953/150‑1 (MQ=255)                                                          tctcCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAGAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACg                                                                                        <  4:524669/150‑1 (MQ=255)                                                            tcCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAg                                                                                      >  1:410508/1‑150 (MQ=255)                                                            tcCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAg                                                                                      >  4:646918/1‑150 (MQ=255)                                                            tcCGCTGAATTGCACAACGCGGACAAGCAACTGCACAGCTACGGATACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGTGAGAACGTATTTTTCCTGCGTTACCGCCACGTATCTCTGCTGCGACCCACAACGAg                                                                                      >  4:407717/1‑150 (MQ=255)                                                              cGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCt                                                                                    >  3:610455/1‑150 (MQ=255)                                                               gCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCGCGTATGTCTGCTGCGGCCCACAACGTGCTa                                                                                   >  2:604503/1‑150 (MQ=255)                                                                cTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTaa                                                                                  <  1:510226/150‑1 (MQ=255)                                                                 tGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAAc                                                                                 <  1:943147/150‑1 (MQ=255)                                                                   aaTTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGa                                                                               >  1:947073/1‑150 (MQ=255)                                                                     ttGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGaaa                                                                             <  3:422618/150‑1 (MQ=255)                                                                     ttGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGaaa                                                                             <  3:422732/150‑1 (MQ=255)                                                                     ttGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGaaa                                                                             >  1:407287/1‑150 (MQ=255)                                                                        cacaACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACtt                                                                          >  2:1108143/1‑150 (MQ=255)                                                                        cacaACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACtt                                                                          >  2:154407/1‑150 (MQ=255)                                                                           aaCGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGc                                                                       <  1:807097/150‑1 (MQ=255)                                                                             cgcgGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGt                                                                     >  4:183680/1‑150 (MQ=255)                                                                               cgGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTc                                                                   <  2:410508/150‑1 (MQ=255)                                                                               cgGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTc                                                                   <  1:1216374/150‑1 (MQ=255)                                                                                ggTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTcg                                                                  >  2:878331/1‑150 (MQ=255)                                                                                 gTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTcgc                                                                 >  3:638417/1‑150 (MQ=255)                                                                                    aaGCAACTGCACAGCTACGGTTACAAAGGTCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTg                                                              <  4:712847/150‑1 (MQ=255)                                                                                    aaGCAACTGCACAGCTACGGTTACAAAGGCCTGTCCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTg                                                              >  4:762890/1‑150 (MQ=255)                                                                                      gCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCt                                                            >  4:339518/1‑150 (MQ=255)                                                                                       cAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTg                                                           <  4:179695/150‑1 (MQ=255)                                                                                         aCTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGAc                                                         >  4:399359/1‑150 (MQ=255)                                                                                           tGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGc                                                       <  2:335021/150‑1 (MQ=255)                                                                                           tGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGc                                                       >  2:341686/1‑150 (MQ=255)                                                                                           tGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGc                                                       >  3:770065/1‑150 (MQ=255)                                                                                                 gCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCa                                                 >  4:756705/1‑150 (MQ=255)                                                                                                 gCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCa                                                 <  4:267753/150‑1 (MQ=255)                                                                                                  cTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCGAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCAt                                                <  2:1170237/150‑1 (MQ=255)                                                                                                   tACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATg                                               <  1:604503/150‑1 (MQ=255)                                                                                                      ggTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTc                                            >  1:382710/1‑150 (MQ=255)                                                                                                          aCAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCa                                        <  2:801649/150‑1 (MQ=255)                                                                                                                accTGACCAACGAAACCCTGAACGTAAAGATAGAACAATCGGGAGATCGTATTTTGCCTGCGTTACCGCCACGTATCTCTTCTGCGGCCCACAACGAGCTAACGAAACTTAGCGTTCGCTTTCTGACTCAAACCATGGTCTCCAGTGCtg                                  >  4:384561/2‑150 (MQ=255)                                                                                                                 ccTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTGTCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCtga                                 <  2:384540/150‑1 (MQ=255)                                                                                                                  cTGACCAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCTTGCTGACGCAAACCATGGTCACCAGTGCtgat                                <  3:384561/150‑1 (MQ=255)                                                                                                                       cAACGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAgg                           <  1:127471/150‑1 (MQ=255)                                                                                                                        aaCGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGGAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAggc                          >  2:762167/1‑150 (MQ=255)                                                                                                                         aCGAAGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTAGTTTTCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAggcg                         <  1:851774/150‑1 (MQ=255)                                                                                                                             aGCCCTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCt                     <  3:756705/150‑1 (MQ=255)                                                                                                                               cccTGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGc                   <  1:830057/150‑1 (MQ=255)                                                                                                                                  tGAACGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGcaca                >  3:616695/1‑150 (MQ=255)                                                                                                                                    aaCGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACt              >  1:911543/1‑150 (MQ=255)                                                                                                                                    aaCGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACt              >  3:310845/1‑150 (MQ=255)                                                                                                                                      cGTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTaa            <  3:184386/150‑1 (MQ=255)                                                                                                                                       gTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTaaa           <  1:1108143/150‑1 (MQ=255)                                                                                                                                       gTAACGCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTCATGAAGGCGGACTGCACACTaaa           >  2:725836/1‑150 (MQ=255)                                                                                                                                            gCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATgg      >  2:538567/1‑150 (MQ=255)                                                                                                                                            gCTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATgg      >  1:1062471/1‑150 (MQ=255)                                                                                                                                             cTGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGc     >  2:549133/1‑150 (MQ=255)                                                                                                                                             cTGGTACAAGCGGCGGGACGTATTTTGCCTGCTTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGTCGTTCGCTGGCTGACGCAAACCGTGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGc     <  4:187518/150‑1 (MQ=255)                                                                                                                                              tGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCg                                                                                                  <  2:582311/56‑1 (MQ=255)                                                                                                                                              tGGTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCg                                                                                                  >  1:582311/1‑56 (MQ=255)                                                                                                                                                gTACAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGaa  <  4:676212/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GCGCGAATGGCAAAGTGAACATTGCGATTGTCGGCGGCGGCGCGACGGGTGTAGAACTCTCCGCTGAATTGCACAACGCGGTCAAGCAACTGCACAGCTACGGTTACAAAGGCCTGACCAACGAAGCCCTGAACGTAACGCTGGTAGAAGCGGGAGAACGTATTTTGCCTGCGTTACCGCCACGTATCTCTGCTGCGGCCCACAACGAGCTAACGAAACTTGGCGTTCGCGTGCTGACGCAAACCATGGTCACCAGTGCTGATGAAGGCGGCCTGCACACTAAAGATGGCGAA  >  NC_000913_3_pae_pgi/1166581‑1166873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑