Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 1,399,449 G→T S26R (AGC→AGA fnr ← oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,399,4490GT95.6% 467.3 / 9.2 135S26R (AGC→AGAfnroxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (2/4);  new base T (61/68);  total (63/72)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

Only 100 of 137 total aligned reads displayed.

TACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAG  >  NC_000913_3_pae_pgi/1399304‑1399594
                                                                                                                                                 |                                                                                                                                                 
tACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGa                                                                                                                                               >  4:391163/1‑150 (MQ=255)
   cGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGAtgc                                                                                                                                            <  3:212170/150‑1 (MQ=255)
     gAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGAtgctg                                                                                                                                          >  1:178055/1‑150 (MQ=255)
              gCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTgg                                                                                                                                 <  2:345736/150‑1 (MQ=255)
                   ttatgATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATg                                                                                                                            <  2:651270/146‑1 (MQ=255)
                     gCGATTTCTGTTCCTCACCAGCGTTAAACAGCGTCTAGTCTTTCTCTATACTATTGTTTCGGTTAATGTTATTAACAAGTTACTCTATCTCATGTTCGTTGAGTGTGATCGGGATGCGAAGCTGTCTGATGCTGCAATTCTGTCAATgta                                                                                                                          <  2:1620517/150‑3 (MQ=255)
                     gCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGa                                                                                                                          >  1:287681/1‑150 (MQ=255)
                     gCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTgg                                                                                                                                 <  4:232252/143‑1 (MQ=255)
                     gCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTgg                                                                                                                                 >  3:232252/1‑143 (MQ=255)
                               ttCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAAcc                                                                                                                >  1:1305748/1‑150 (MQ=255)
                               ttCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAAcc                                                                                                                >  3:153072/1‑150 (MQ=255)
                                       cAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACt                                                                                                        <  2:1651515/150‑1 (MQ=255)
                                               aaCAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATgcgc                                                                                                <  4:277413/150‑1 (MQ=255)
                                                 cAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTTTCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGACCGCGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGTATAGCACAACCGCCAGACTGAATGCGCCg                                                                                              <  1:1023302/150‑1 (MQ=255)
                                                 cAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCg                                                                                              >  1:1786521/1‑150 (MQ=255)
                                                       cTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAAt                                                                                        <  2:1446911/150‑1 (MQ=255)
                                                          gCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCg                                                                                     <  1:1153672/150‑1 (MQ=255)
                                                            cTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCt                                                                                   >  3:363729/1‑150 (MQ=255)
                                                             tttCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCtt                                                                                  >  2:487320/1‑150 (MQ=255)
                                                                  gAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAAt                                                                                                                             >  1:115201/1‑102 (MQ=255)
                                                                  gAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAAt                                                                                                                             <  2:115201/102‑1 (MQ=255)
                                                                  gAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCg                                                                             <  2:287681/150‑1 (MQ=255)
                                                                   aaTAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCgg                                                                            <  3:214268/150‑1 (MQ=255)
                                                                   aaTAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGGTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCgg                                                                            <  3:771607/150‑1 (MQ=255)
                                                                    aTAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCggg                                                                           <  2:178055/150‑1 (MQ=255)
                                                                      aGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGAt                                                                         <  2:37762/150‑1 (MQ=255)
                                                                        gCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCa                                                                       >  2:1205082/1‑150 (MQ=255)
                                                                        gCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCa                                                                       >  2:222477/1‑150 (MQ=255)
                                                                        gCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCa                                                                       >  3:7877/1‑150 (MQ=255)
                                                                           tcttcCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAg                                                                    <  1:1469050/150‑1 (MQ=255)
                                                                             ttcCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGc                                                                                                                                <  3:544462/88‑1 (MQ=255)
                                                                              tcCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTc                                                                 <  3:710969/150‑1 (MQ=255)
                                                                                cGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTg                                                               <  2:418648/150‑1 (MQ=255)
                                                                                 gCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATGATAGGTCTGc                                                              >  1:1324204/1‑150 (MQ=255)
                                                                                 gCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGc                                                              <  1:523728/150‑1 (MQ=255)
                                                                                 gCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGc                                                              <  1:488715/150‑1 (MQ=255)
                                                                                   tCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCATAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTc                                                            >  2:1354917/1‑150 (MQ=255)
                                                                                   tCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGtat                                                                                           >  2:1419903/1‑119 (MQ=255)
                                                                                   tCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGtat                                                                                           <  1:1419903/119‑1 (MQ=255)
                                                                                     aaTGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGa                                                                                                                          >  1:1457649/1‑86 (MQ=255)
                                                                                     aaTGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTGTAATTCGCTTTTCCGGGATCATAGGTCTGCTCaa                                                          >  3:1045831/1‑150 (MQ=255)
                                                                                     aaTGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCaa                                                          >  2:817516/1‑150 (MQ=255)
                                                                                     aaTGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCaa                                                          >  3:767338/1‑150 (MQ=255)
                                                                                       tGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATa                                                                     >  1:1694334/1‑137 (MQ=255)
                                                                                       tGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGc                                                        >  2:1719773/1‑150 (MQ=255)
                                                                                         atatTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCg                                                      <  2:1305748/150‑1 (MQ=255)
                                                                                            ttatCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATa                                                                                                                        >  1:1377911/1‑81 (MQ=255)
                                                                                             tatCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAAt                                                  >  2:1039068/1‑150 (MQ=255)
                                                                                              atCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAAtt                                                 <  4:363729/150‑1 (MQ=255)
                                                                                              atCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGCCTGCTCAAGCCGTAAtt                                                 <  3:661294/150‑1 (MQ=255)
                                                                                                  aGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACt                                                                                                        >  2:966215/1‑91 (MQ=255)
                                                                                                  aGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACt                                                                                                        <  1:966215/91‑1 (MQ=255)
                                                                                                  aGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGata                                             <  1:817516/150‑1 (MQ=255)
                                                                                                   gCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGatat                                            <  3:83351/150‑1 (MQ=255)
                                                                                                   gCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGatat                                            <  3:385445/150‑1 (MQ=255)
                                                                                                     tGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGAtgctgc                                                                                                                                         >  1:40748/1‑55 (MQ=255)
                                                                                                     tGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATAttt                                          >  4:963884/1‑150 (MQ=255)
                                                                                                     tGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATAttt                                          <  2:127150/150‑1 (MQ=255)
                                                                                                       aTCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGt                                        >  2:735449/1‑150 (MQ=255)
                                                                                                       aTCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGt                                        >  4:1076698/1‑150 (MQ=255)
                                                                                                         cAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTgg                                                                                                                                 >  1:1519695/1‑59 (MQ=255)
                                                                                                         cAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTgg                                                                                                                                 <  2:1519695/59‑1 (MQ=255)
                                                                                                                aTGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTaa                               <  1:977387/150‑1 (MQ=255)
                                                                                                                aTGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTaa                               <  4:48626/150‑1 (MQ=255)
                                                                                                                aTGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTaa                               >  1:1257969/1‑150 (MQ=255)
                                                                                                                 tGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAAc                              <  3:1076698/150‑1 (MQ=255)
                                                                                                                      gTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGc                                                                                                                                >  4:68655/1‑47 (MQ=255)
                                                                                                                      gTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGc                                                                                                                                <  3:68655/47‑1 (MQ=255)
                                                                                                                      gTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCaa                                                          <  2:846195/117‑1 (MQ=255)
                                                                                                                      gTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCaa                                                          >  1:846195/1‑117 (MQ=255)
                                                                                                                      gTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCtt                         >  1:1075833/1‑150 (MQ=255)
                                                                                                                       ttGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCttt                        >  4:1020001/1‑150 (MQ=255)
                                                                                                                       ttGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCttt                        <  2:1335892/150‑1 (MQ=255)
                                                                                                                        tGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCtttt                       <  1:222477/150‑1 (MQ=255)
                                                                                                                         gAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCttttt                      <  1:1039068/150‑1 (MQ=255)
                                                                                                                          aGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTa                     <  1:41590/150‑1 (MQ=255)
                                                                                                                            tgtgAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAgg                   <  1:238398/150‑1 (MQ=255)
                                                                                                                              tgAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGa                 >  2:765168/1‑150 (MQ=255)
                                                                                                                              tgAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGa                 >  2:1553331/1‑150 (MQ=255)
                                                                                                                               gAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAAtt                                   >  4:956563/1‑131 (MQ=255)
                                                                                                                               gAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAAtt                                   <  3:956563/131‑1 (MQ=255)
                                                                                                                               gAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAg                >  3:388901/1‑150 (MQ=255)
                                                                                                                               gAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAg                >  2:1160864/1‑150 (MQ=255)
                                                                                                                                aaCGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGc               <  1:1205082/150‑1 (MQ=255)
                                                                                                                                  cGGGATGCACAGCTGTCTGATGCTGCAATCCTGGAAATGGATAGCACACCCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCaa             <  4:751722/150‑1 (MQ=255)
                                                                                                                                     gATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAAcc                                                                                                                <  2:1382878/48‑1 (MQ=255)
                                                                                                                                     gATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCaagta          <  4:706899/150‑1 (MQ=255)
                                                                                                                                      aTGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCATAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCaagtaa         <  1:1354917/150‑1 (MQ=255)
                                                                                                                                      aTGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCaagtaa         <  1:735482/150‑1 (MQ=255)
                                                                                                                                      aTGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCaagtaa         <  2:1257969/150‑1 (MQ=255)
                                                                                                                                        gCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGa                                                                                                          >  1:512471/1‑51 (MQ=255)
                                                                                                                                        gCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGa                                                                                                          <  2:512471/51‑1 (MQ=255)
                                                                                                                                        gCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCaagtaagt       >  3:180011/1‑150 (MQ=255)
                                                                                                                                         cAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTc      <  4:180011/150‑1 (MQ=255)
                                                                                                                                         cAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTc      >  2:1665410/1‑150 (MQ=255)
                                                                                                                                          aaaGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATCTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCt     >  3:254151/1‑150 (MQ=255)
                                                                                                                                            aGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTaa   <  1:1569059/150‑1 (MQ=255)
                                                                                                                                            aGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTCCCGGGATCATAGGTCCGCCCAAGCCGTAATTGATATTTGTCACTTTTAACATCTTTTTAGGGAGCAAGTACGTCTaa   >  4:151558/1‑150 (MQ=255)
                                                                                                                                             gctgtctgATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTCACATCTTTTTAGGGAGCAAGTAAGTCTAAg  <  2:926189/150‑1 (MQ=255)
                                                                                                                                             gctgtctgATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTATTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAg  >  3:71066/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTCTAAG  >  NC_000913_3_pae_pgi/1399304‑1399594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: