| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_pgi | 225,465 | 0 | A | . | 50.0% | 3.3 / 23.1 | 11 | intergenic (+8/‑35) | ileV/alaV | tRNA‑Ile/tRNA‑Ala |
| Reads supporting (aligned to +/- strand): ref base A (2/3); new base . (4/1); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGTCCACTCAGGCCTACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTG > NC_000913_3_pae_pgi/225439‑225611 | aGTCAACTCAGGACTACCAACTTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACAAGTTATGAGGCTAAAGCTAAGCTGGGAGAGCACCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATCGCTCCACCCTACCTGTAGTGGt > 2:136520/1‑150 (MQ=11) gcTCGGGCCTACCAAATGTGCACG‑GAAGATGTGAAGAGGTTTTAACTACATGTTATGGGGCTAGAGCTCAGTTGGGAGAGCGCGTGCTTTGCACGCGGGAGGTCTTCGGTTAGATCCCGCATTGCTCCACATTCTCTGTAGTGGTtaaat < 2:230194/149‑1 (MQ=11) aGGCCTACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATaaaa < 2:104397/150‑1 (MQ=32) aGGCCTACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATaaaa > 2:540006/1‑150 (MQ=32) ctcttcAAATTTGCCGT‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAAt > 2:479353/6‑150 (MQ=11) ttcAAATTTGC‑CGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGtt > 1:428610/3‑136 (MQ=255) ttcAAATTTGC‑CGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGtt < 2:428610/134‑1 (MQ=255) tcAAATTTGC‑CGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACtt > 1:135343/2‑150 (MQ=255) tcAAATTTGC‑CGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACtt > 1:663928/2‑150 (MQ=255) tcAAATTTGC‑CGTGCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACtt > 1:99895/2‑150 (MQ=255) tGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAgtg < 1:177498/150‑1 (MQ=32) | AGTCCACTCAGGCCTACCAAATTTGCACG‑GCAAATTTGAAGAGGTTTTAACTACATGTTATGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCTGCGGTTCGATCCCGCATAGCTCCACCATCTCTGTAGTGGTTAAATAAAAAATACTTCAGAGTG > NC_000913_3_pae_pgi/225439‑225611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |