Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 4,159,061 T→G L191R (CTG→CGG)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi4,159,0610TG98.2% 188.9 / ‑4.7 54L191R (CTG→CGG) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (30/23);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACT  >  NC_000913_3_pae_pgi/4158919‑4159203
                                                                                                                                              |                                                                                                                                              
gTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATg                                                                                                                                         >  3:92761/1‑150 (MQ=255)
  gATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGAGGGCCGACCTGGCAGGGGAAAAACGgctgctgct                                                                                                                                       >  2:181051/1‑150 (MQ=255)
   aTCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTg                                                                                                                                      >  2:158533/1‑150 (MQ=255)
    tCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTgg                                                                                                                                     <  4:141246/150‑1 (MQ=255)
      cTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGaa                                                                                                                                   >  3:133056/1‑150 (MQ=255)
       tCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAg                                                                                                                                  >  1:515073/1‑150 (MQ=255)
       gcgcgCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGGGGTGGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAg                                                                                                                                  <  1:545623/149‑1 (MQ=255)
          cgcTGGTGCAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTAGCTAGGAAGATCACCCGTGGGCGAGCCGCGAAGGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATg                                                                                                                               <  2:206569/150‑1 (MQ=255)
             tGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTc                                                                                                                            <  1:329119/150‑1 (MQ=255)
                         gCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGcgcg                                                                                                                <  3:317133/150‑1 (MQ=255)
                           gAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGAt                                                                                                              >  1:333021/1‑150 (MQ=255)
                            aaGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATc                                                                                                             <  3:244470/150‑1 (MQ=255)
                                 ttcattGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCa                                                                                                        >  2:661184/1‑150 (MQ=255)
                                 ttcattGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCa                                                                                                        >  3:37443/1‑150 (MQ=255)
                                    attGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATg                                                                                                     <  1:196843/150‑1 (MQ=255)
                                       gAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGt                                                                                                  >  2:207931/1‑150 (MQ=255)
                                              cGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtt                                                                                           >  2:89319/1‑150 (MQ=255)
                                              cGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtt                                                                                           >  2:353866/1‑150 (MQ=255)
                                                ttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAAcggc                                                                                                                                              <  1:268755/97‑1 (MQ=255)
                                                ttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAAcggc                                                                                                                                              <  1:268670/97‑1 (MQ=255)
                                                ttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAAcggc                                                                                                                                              >  2:268755/1‑97 (MQ=255)
                                                ttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGtttt                                                                                         <  4:256732/150‑1 (MQ=255)
                                                      gatgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGcc                                                                                   <  1:158533/150‑1 (MQ=255)
                                                        tgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgg                                                                                 <  4:92761/150‑1 (MQ=255)
                                                           gCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGGTTCTGTTTTGAAGCCGGGGc                                                                              >  2:260200/1‑150 (MQ=255)
                                                           gCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGTTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGc                                                                              >  3:169755/1‑150 (MQ=255)
                                                           gCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGGCGGGGc                                                                              <  1:440580/150‑1 (MQ=255)
                                                           gCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGc                                                                              <  1:28984/150‑1 (MQ=255)
                                                           gCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGc                                                                              >  2:440580/1‑150 (MQ=255)
                                                               aTGTTGCTGGCTATCTATGAAGATCACCCGTGGGTGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGAt                                                                          <  2:390316/150‑1 (MQ=255)
                                                                 gTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGa                                                                        <  2:270961/150‑1 (MQ=255)
                                                                     cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAgg                                                                                                          >  2:99725/1‑112 (MQ=255)
                                                                     cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAgg                                                                                                          <  1:99725/112‑1 (MQ=255)
                                                                        gCTATCTCTGAAGATCACCCGTGGGCGAGCCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATaca                                                                 <  4:40048/150‑1 (MQ=255)
                                                                        gCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATaca                                                                 >  2:237420/1‑150 (MQ=255)
                                                                               atGAAGATCACCCGTGCGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTcc                                                          >  2:358883/1‑150 (MQ=255)
                                                                                      tCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGAc                                                   >  3:248105/1‑150 (MQ=255)
                                                                                        aCCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCa                                                 >  2:548419/1‑150 (MQ=255)
                                                                                             tGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTg                                            >  1:213668/1‑150 (MQ=255)
                                                                                             tGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTg                                            <  4:381660/150‑1 (MQ=255)
                                                                                                     ccGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAActct                                    >  2:327401/1‑150 (MQ=255)
                                                                                                      cgcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTg                                   >  3:130760/1‑150 (MQ=255)
                                                                                                         gAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTACCGCGCGACCAGCCTGGAAACTCTgcgc                                >  3:378434/1‑150 (MQ=255)
                                                                                                             gCGTACCGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACa                            <  4:37443/150‑1 (MQ=255)
                                                                                                                   cGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtgg                      <  2:197156/150‑1 (MQ=255)
                                                                                                                   cGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtgg                      >  4:171919/1‑150 (MQ=255)
                                                                                                                   cGATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtgg                      <  1:328618/150‑1 (MQ=255)
                                                                                                                    gATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcg                                                         <  1:418633/114‑1 (MQ=255)
                                                                                                                    gATGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggc                     >  2:479375/1‑150 (MQ=255)
                                                                                                                    gATGGCCGATCTGGCAGGAGAAAAACGGCTGATGCTGGAAGATGGTCCCTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcg                                                         >  2:418633/1‑114 (MQ=255)
                                                                                                                     aTGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcg                    >  2:609007/1‑150 (MQ=255)
                                                                                                                      tGGCCGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcgg                   <  3:171919/150‑1 (MQ=255)
                                                                                                                         ccGATCTGGCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAg                >  2:674596/1‑150 (MQ=255)
                                                                                                                                 gCAGGGGAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCggg        >  4:285410/1‑150 (MQ=255)
                                                                                                                                 gCAGGGGAAAAACGGCGGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCGGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTCCGCAACATGGTGGCGGCAGGTAGCggg        >  4:427342/1‑150 (MQ=255)
                                                                                                                                    ggggAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATc     >  2:443338/1‑150 (MQ=255)
                                                                                                                                       gAAAAACGGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACt  <  1:674596/150‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                              
GTGATCCTCGCGCTGGTGAAAGAGAGCGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACT  >  NC_000913_3_pae_pgi/4158919‑4159203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑