Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 2,995,873 C→T pseudogene (148/261 nt) ygeN ← pseudogene, orgB family, part of T3SS PAI ETT2 remnant

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi2,995,8730CT100.0% 313.8 / NA 87pseudogene (148/261 nt)ygeNpseudogene, orgB family, part of T3SS PAI ETT2 remnant
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (47/40);  total (47/40)

TTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGC  >  NC_000913_3_pae_pgi/2995729‑2996018
                                                                                                                                                |                                                                                                                                                 
ttCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCa                                                                                                                                               >  3:146300/1‑149 (MQ=255)
ttCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCa                                                                                                                                               <  4:146300/149‑1 (MQ=255)
     aTCGTTTATCATGTTTATTCTTTTTTTTTTTTTTTTTAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATActc                                                                                                                                         >  2:120080/1‑150 (MQ=255)
     aTCGTTTATCATGTTTATTCTTTTTTTTTTTTTTTTTAACATAGAAAGCTTATCAAGAGTATAAAATTATCCATCTCATCAATAATGCCTTTTCTATAGCCATCATAATAACCTTGAATAAATTTTTCTTTTTTCTTTTTCTCAATActt                                                                                                                                         >  4:300512/1‑149 (MQ=255)
           tATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAAtt                                                                                                                                   >  2:830307/1‑150 (MQ=255)
                  tttATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGc                                                                                                                              >  4:71006/1‑148 (MQ=255)
                  tttATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGc                                                                                                                              <  3:71006/148‑1 (MQ=255)
                  tttATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCgg                                                                                                                            >  1:1501/1‑150 (MQ=255)
                    tATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCggtg                                                                                                                          <  3:300512/150‑1 (MQ=255)
                    tATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCggtg                                                                                                                          <  3:273313/150‑1 (MQ=255)
                      ttCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCggtggt                                                                                                                        >  3:177477/1‑150 (MQ=255)
                         ttttttttCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCggtggt                                                                                                                        <  4:561919/147‑1 (MQ=255)
                         ttttttttCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCggtggt                                                                                                                        >  3:561919/1‑147 (MQ=255)
                               ttCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCa                                                                                                                                               >  3:442080/1‑118 (MQ=255)
                               ttCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCa                                                                                                                                               <  4:442080/118‑1 (MQ=255)
                               ttCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCaagaag                                                                                                               >  1:41050/1‑150 (MQ=255)
                                       cTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGAtttttt                                                                                                       <  4:571816/150‑1 (MQ=255)
                                         gAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCt                                                                                                     <  4:223127/150‑1 (MQ=255)
                                               aGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAaga                                                                                               >  1:256382/1‑150 (MQ=255)
                                                gCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTTTATTTTCTTCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAagag                                                                                              >  2:919212/1‑150 (MQ=255)
                                                gCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAagag                                                                                              >  4:332383/1‑150 (MQ=255)
                                                 cAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAgaga                                                                                             <  1:830307/150‑1 (MQ=255)
                                                 cAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAgaga                                                                                             >  4:198173/1‑150 (MQ=255)
                                                        aTCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTAtt                                                                                      <  1:42755/150‑1 (MQ=255)
                                                          cAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTAtttc                                                                                    >  3:336990/1‑150 (MQ=255)
                                                           aaGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTAtttct                                                                                   >  1:16943/1‑150 (MQ=255)
                                                             gggTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAAttttt                                                                                                                                >  2:979226/1‑103 (MQ=255)
                                                             gggTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAAttttt                                                                                                                                <  1:979226/103‑1 (MQ=255)
                                                             gggTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTAtttcttt                                                                                 >  1:152813/1‑150 (MQ=255)
                                                               gTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCa                                                                               <  2:152813/150‑1 (MQ=255)
                                                                             aTCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCATTACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCt                                                                                                     <  4:302893/114‑1 (MQ=255)
                                                                             aTCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCt                                                                                                     >  3:302893/1‑114 (MQ=255)
                                                                              tctcATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAAttt                                                                >  1:436801/1‑150 (MQ=255)
                                                                              tctcATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAAttt                                                                <  4:336990/150‑1 (MQ=255)
                                                                               ctcATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAAtttt                                                               <  2:256382/150‑1 (MQ=255)
                                                                                  atcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCAt                                                                         >  3:115279/1‑137 (MQ=255)
                                                                                  atcaATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCAt                                                                         <  4:115279/137‑1 (MQ=255)
                                                                                     aataatGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCtttt                                                         >  4:344662/1‑150 (MQ=255)
                                                                                      ataatGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTAtttcttt                                                                                 <  3:133722/125‑1 (MQ=255)
                                                                                      ataatGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTAtttcttt                                                                                 >  4:133722/1‑125 (MQ=255)
                                                                                         atGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATc                                                     >  4:419484/1‑150 (MQ=255)
                                                                                           gCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTAtttcttt                                                                                 <  1:642296/120‑1 (MQ=255)
                                                                                           gCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTAtttcttt                                                                                 >  2:642296/1‑120 (MQ=255)
                                                                                           gCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATcac                                                   >  1:721415/1‑150 (MQ=255)
                                                                                            ccTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCAt                                                                         <  2:177737/127‑1 (MQ=255)
                                                                                            ccTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATcaca                                                  <  2:853002/150‑1 (MQ=255)
                                                                                            ccTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTCCTTTAAGAGAAACTATTTCTTTCAATCCAt                                                                         >  1:177737/1‑127 (MQ=255)
                                                                                              ttttGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCacaac                                                >  4:27390/1‑150 (MQ=255)
                                                                                               tttGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCacaaca                                               <  2:720986/150‑1 (MQ=255)
                                                                                                  gTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTc                                                             <  3:148698/133‑1 (MQ=255)
                                                                                                  gTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTc                                                             >  4:148698/1‑133 (MQ=255)
                                                                                                     taGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCAttt                                         <  3:27390/150‑1 (MQ=255)
                                                                                                     taGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAAAACCAttt                                         >  3:610808/1‑150 (MQ=255)
                                                                                                        ccATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCa                                      >  1:1000619/1‑150 (MQ=255)
                                                                                                          atcatAATAACCTTGAATAAATTTTTCTTCTATCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAAAAATTTTTCTTTTAATAACAACACCATTTGCAgg                                    >  4:249901/1‑150 (MQ=255)
                                                                                                           tcatAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCacaaca                                               <  4:138009/138‑1 (MQ=255)
                                                                                                           tcatAATAACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCacaaca                                               >  3:138009/1‑138 (MQ=255)
                                                                                                             ataataACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCt                                                                                                     <  1:867951/82‑1 (MQ=255)
                                                                                                             ataataACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCt                                                                                                     >  2:867951/1‑82 (MQ=255)
                                                                                                             ataataACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCttt                                                          >  1:124558/1‑125 (MQ=255)
                                                                                                             ataataACCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCttt                                                          <  2:124558/125‑1 (MQ=255)
                                                                                                              taataaCCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAAc                                                                                          >  1:363643/1‑92 (MQ=255)
                                                                                                              taataaCCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAAc                                                                                          <  2:363643/92‑1 (MQ=255)
                                                                                                              taataaCCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGAt                                <  3:419484/150‑1 (MQ=255)
                                                                                                               aataaCCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTAtttctt                                                                                  <  2:708614/99‑1 (MQ=255)
                                                                                                               aataaCCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTAtttctt                                                                                  >  1:708614/1‑99 (MQ=255)
                                                                                                                ataaCCTTGAATAAATTTTTCTTCTCTCTTTTTCTTTATATTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTATGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAggtttttt                              >  4:278293/1‑144 (MQ=255)
                                                                                                                 taaCCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGAtttt                             <  1:120080/150‑1 (MQ=255)
                                                                                                                   aCCTTGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTAt                           <  3:198173/150‑1 (MQ=255)
                                                                                                                      ttGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTAttt                                                                                     <  3:440723/89‑1 (MQ=255)
                                                                                                                      ttGAATAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTAttt                                                                                     >  4:440723/1‑89 (MQ=255)
                                                                                                                           tAAATTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCa                   <  4:177477/150‑1 (MQ=255)
                                                                                                                            aaaTTTTTCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCAt                  <  3:212790/150‑1 (MQ=255)
                                                                                                                                ttttCTTCTCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATctct              <  3:278293/150‑1 (MQ=255)
                                                                                                                                     ttctCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCacaa                                                 >  2:824937/1‑110 (MQ=255)
                                                                                                                                     ttctCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCacaa                                                 <  1:824937/110‑1 (MQ=255)
                                                                                                                                     ttctCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtttt         <  3:344662/150‑1 (MQ=255)
                                                                                                                                     ttctCTCTTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtttt         >  1:435911/1‑150 (MQ=255)
                                                                                                                                        tctctTTTTCTCTTTACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAttttttt      >  2:316392/1‑150 (MQ=255)
                                                                                                                                        tctctTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCa                                      >  2:380676/1‑118 (MQ=255)
                                                                                                                                        tctctTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCa                                      <  1:380676/118‑1 (MQ=255)
                                                                                                                                        tctctTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAttttttt      >  3:424639/1‑150 (MQ=255)
                                                                                                                                         ctctTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTAtttttttt     <  2:1000619/150‑1 (MQ=255)
                                                                                                                                          tctTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAAt                                                      >  4:509050/1‑100 (MQ=255)
                                                                                                                                          tctTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAAt                                                      <  3:509050/100‑1 (MQ=255)
                                                                                                                                          tctTTTTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTc    >  1:711785/1‑150 (MQ=255)
                                                                                                                                            ttttTCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGc  >  1:727519/1‑150 (MQ=255)
                                                                                                                                             ttttCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCAt                                                                         >  1:101827/1‑78 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TTCAGATCGTTTATCATGTTTATTCTTTTTTTTCAAGTTCTGAGCATAGCAAGCTTATCAAGGGTATAAAATTATCCATCTCATCAATAATGCCTTTTGTATAGCCATCATAATAACCTTGAATAAATTTTTCTTCTCTCTTTTCCTCAATACTCTGAATTTTTGCGGTGGTTTCAAGAAGGATTTTTTCTTTAAGAGAAACTATTTCTTTCAATCCATCAGAAATTTTTCTTTTAATCACAACACCATTTGCAGGAGATTTTATCAAGCTCATCTCTATTTTTTTTCGC  >  NC_000913_3_pae_pgi/2995729‑2996018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: