Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 3,907,683 G→T G228G (GGC→GGA pstB ← phosphate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi3,907,6830GT99.2% 416.2 / NA 117G228G (GGC→GGApstBphosphate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (56/60);  minor base C (0/1);  total (56/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

TGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGA  >  NC_000913_3_pae_pgi/3907536‑3907823
                                                                                                                                                   |                                                                                                                                            
tGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTcc                                                                                                                                            <  2:867730/150‑1 (MQ=255)
tGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTcc                                                                                                                                            <  2:250492/150‑1 (MQ=255)
  aaCTGGCCGGAAATATGTTTATTAAGATTGAGGCTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGCGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCa                                                                                                                                          <  3:127154/150‑1 (MQ=255)
  aaCTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCa                                                                                                                                          <  2:158274/150‑1 (MQ=255)
  aaCTGGCCGGAAATATGTTTATTAAGATTGAGACTGTACATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCa                                                                                                                                          >  3:335413/1‑150 (MQ=255)
     tGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGt                                                                                                                                       <  2:783912/150‑1 (MQ=255)
                  gTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCgt                                                                                                                          >  2:920134/1‑150 (MQ=255)
                   tttATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGcc                                                                                                                            <  2:944978/147‑1 (MQ=255)
                    ttattaAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCgtgt                                                                                                                        <  1:274454/150‑1 (MQ=255)
                      attaAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTgg                                                                                                                      >  2:676574/1‑150 (MQ=255)
                       ttaAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGt                                                                                                                     <  1:39610/150‑1 (MQ=255)
                        taAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTc                                                                                                                    >  3:278121/1‑150 (MQ=255)
                          aGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCgg                                                                                                                  <  4:137150/150‑1 (MQ=255)
                            aTTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGaa                                                                                                                >  4:304995/1‑150 (MQ=255)
                                  aCTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAAcgc                                                                                                          <  1:853635/150‑1 (MQ=255)
                                   cTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAAcgcg                                                                                                         <  1:920134/150‑1 (MQ=255)
                                      tCCATAACGCACTCCTGAATCAACCGTAACAACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAg                                                                                                      >  1:130694/1‑150 (MQ=255)
                                        cATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGcc                                                                                                    >  1:952730/1‑150 (MQ=255)
                                         aTAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACAt                                                                                                                                   >  1:616694/1‑118 (MQ=255)
                                         aTAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACAt                                                                                                                                   <  2:616694/118‑1 (MQ=255)
                                            aCGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCctgct                                                                                                >  3:190446/1‑150 (MQ=255)
                                             cGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTc                                                                                                                    >  4:386824/1‑129 (MQ=255)
                                             cGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTc                                                                                                                    <  3:387001/129‑1 (MQ=255)
                                             cGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTc                                                                                                                    >  4:387001/1‑129 (MQ=255)
                                             cGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTc                                                                                                                    <  3:386824/129‑1 (MQ=255)
                                                   ccTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCAtgtt                                                                                         >  2:404227/1‑150 (MQ=255)
                                                   ccTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCAtgtt                                                                                         <  3:304995/150‑1 (MQ=255)
                                                     tGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGa                                                                                                                 <  1:323241/124‑1 (MQ=255)
                                                     tGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGa                                                                                                                 >  2:323241/1‑124 (MQ=255)
                                                      gAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCa                                                                                                                                          <  4:333577/98‑1 (MQ=255)
                                                      gAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCa                                                                                                                                          >  3:333577/1‑98 (MQ=255)
                                                       aaTCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTgg                                                                                     <  4:348164/150‑1 (MQ=255)
                                                       aaTCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCCCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTgg                                                                                     <  2:358998/150‑1 (MQ=255)
                                                        aTCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCAtgttgt                                                                                       >  1:982941/1‑147 (MQ=255)
                                                        aTCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCAtgttgt                                                                                       <  2:982941/147‑1 (MQ=255)
                                                         tCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCCGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGt                                                                                   <  1:223044/150‑1 (MQ=255)
                                                          cAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAAcgcg                                                                                                         <  1:268405/127‑1 (MQ=255)
                                                          cAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAAcgcg                                                                                                         >  2:268405/1‑127 (MQ=255)
                                                              cGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTc                                                                                                                    >  2:271820/1‑112 (MQ=255)
                                                               gTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGAt                                                                             >  2:624045/1‑150 (MQ=255)
                                                                 aaCGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGt                                                                                   >  1:561692/1‑142 (MQ=255)
                                                                 aaCGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGt                                                                                   <  2:561692/142‑1 (MQ=255)
                                                                    gACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATcacca                                                                        >  2:946270/1‑150 (MQ=255)
                                                                        ggTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGgt                                                                    >  1:631501/1‑150 (MQ=255)
                                                                         gTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACgg                                                                     >  4:222750/1‑148 (MQ=255)
                                                                         gTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACgg                                                                     <  3:222750/148‑1 (MQ=255)
                                                                                aGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCt                                                            >  2:498678/1‑150 (MQ=255)
                                                                                  tcttctGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACCCAACCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCCTGTTGTGGGTGACGATCACCACGGTGTAATCCTGc                                                          >  4:49545/1‑150 (MQ=255)
                                                                                  tcttctGTTTGTTTCTTCGCGGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGt                                                                                                                     <  1:631068/91‑1 (MQ=255)
                                                                                   cttctGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCt                                                         <  1:676574/150‑1 (MQ=255)
                                                                                    ttctGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCtt                                                        <  2:952730/150‑1 (MQ=255)
                                                                                      ctGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCa                                                      >  1:874783/1‑150 (MQ=255)
                                                                                         tttgtttCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTa                                                                                                                                      <  2:290858/67‑1 (MQ=255)
                                                                                         tttgtttCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTa                                                                                                                                      >  1:290858/1‑67 (MQ=255)
                                                                                         tttgtttCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTgg                                                                                     <  4:355765/116‑1 (MQ=255)
                                                                                         tttgtttCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTgg                                                                                     >  3:355765/1‑116 (MQ=255)
                                                                                         tttgtttCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGtt                                                   <  4:335413/150‑1 (MQ=255)
                                                                                         tttgtttCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGtt                                                   >  2:168198/1‑150 (MQ=255)
                                                                                           tgtttCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCg                                                 <  3:103614/150‑1 (MQ=255)
                                                                                            gtttCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCgg                                                <  2:715439/150‑1 (MQ=255)
                                                                                             tttCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAAcg                                                                                                           >  2:358263/1‑90 (MQ=255)
                                                                                             tttCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAAcg                                                                                                           <  1:358263/90‑1 (MQ=255)
                                                                                             tttCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCt                                                            <  2:58138/137‑1 (MQ=255)
                                                                                             tttCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCt                                                            >  1:58138/1‑137 (MQ=255)
                                                                                                cttcGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACGGCGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGAt                                            <  4:278121/150‑1 (MQ=255)
                                                                                                cttcGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGAt                                            <  1:498678/150‑1 (MQ=255)
                                                                                                 ttcGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACg                                                                      <  1:553545/123‑1 (MQ=255)
                                                                                                  tcGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGaaca                                                                                                              >  4:307000/1‑82 (MQ=255)
                                                                                                  tcGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGaaca                                                                                                              <  3:307000/82‑1 (MQ=255)
                                                                                                  tcGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCg                                                 <  2:37478/143‑1 (MQ=255)
                                                                                                  tcGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCg                                                 >  1:37478/1‑143 (MQ=255)
                                                                                                  tcGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCa                                          <  1:893982/150‑1 (MQ=255)
                                                                                                   cGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAg                                         <  1:404227/150‑1 (MQ=255)
                                                                                                    gCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGc                                        >  2:649801/1‑150 (MQ=255)
                                                                                                        gCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGt                                                                                   >  2:655316/1‑103 (MQ=255)
                                                                                                         cTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGtgtg                                                                                                                       <  3:375855/66‑1 (MQ=255)
                                                                                                             gTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGgtgt                                                                  <  3:561684/115‑1 (MQ=255)
                                                                                                             gTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGgtgt                                                                  >  4:561684/1‑115 (MQ=255)
                                                                                                                aaCAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACg                                                                      <  1:143098/108‑1 (MQ=255)
                                                                                                                aaCAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACg                                                                      >  2:143098/1‑108 (MQ=255)
                                                                                                                  cAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCgg                                                <  1:679028/128‑1 (MQ=255)
                                                                                                                  cAGATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCgg                                                >  2:679028/1‑128 (MQ=255)
                                                                                                                    gATCGTCCGTGTTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCgg                        <  2:130694/150‑1 (MQ=255)
                                                                                                                        gTCCGTGTTGCTGAACTCAGTCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTggg                                                                                    >  4:276077/1‑86 (MQ=255)
                                                                                                                            gtgtTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCctgc                                                                                                 <  4:574301/69‑1 (MQ=255)
                                                                                                                            gtgtTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCctgc                                                                                                 >  3:574301/1‑69 (MQ=255)
                                                                                                                              gtTGCTGAACTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAgg              <  3:30353/150‑1 (MQ=255)
                                                                                                                                     aaCTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAgc       <  4:190446/150‑1 (MQ=255)
                                                                                                                                       cTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATc                                                              >  2:455804/1‑93 (MQ=255)
                                                                                                                                       cTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATc                                                              <  1:455804/93‑1 (MQ=255)
                                                                                                                                       cTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAgcgc     <  1:624045/150‑1 (MQ=255)
                                                                                                                                       cTCAATCAATTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAgcgc     <  1:168198/150‑1 (MQ=255)
                                                                                                                                        tcaatcaaTTCTCCCAGGTACATAAACGCCGTGTGGTCGGaa                                                                                                                >  2:589904/1‑42 (MQ=255)
                                                                                                                                        tcaatcaaTTCTCCCAGGTACATAAACGCCGTGTGGTCGGaa                                                                                                                <  1:589904/42‑1 (MQ=255)
                                                                                                                                        tcaatcaaTTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGcc    >  2:830526/1‑150 (MQ=255)
                                                                                                                                          aatcaatTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGt                                                                                   <  4:215641/69‑1 (MQ=255)
                                                                                                                                          aatcaatTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGt                                                                                   >  3:215641/1‑69 (MQ=255)
                                                                                                                                          aatcaatTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGa  >  1:147978/1‑150 (MQ=255)
                                                                                                                                           atcaatTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGctct                                     <  2:278528/114‑1 (MQ=255)
                                                                                                                                           atcaatTCTCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGctct                                     >  1:278528/1‑114 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
TGAACTGGCCGGAAATATGTTTATTAAGATTGAGACTGTCCATAACGCACTCCTGAATCAACCGTAACGACCGGTGATGTAGTCTTCTGTTTGTTTCTTCGCTGGCTTGGTGAACAGATCGTCCGTGTTGCTGAACTCAATCAATTCGCCCAGGTACATAAACGCCGTGTGGTCGGAACAACGCGCAGCCTGCTGCATGTTGTGGGTGACGATCACCACGGTGTAATCCTGCTTCAGTTCGGTGATCAGCTCTTCAATACGCCCGGTAGAGATAGGGTCGAGCGCCGA  >  NC_000913_3_pae_pgi/3907536‑3907823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: