Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi2,596,8730C.21.3% 576.0 / 184.9 212intergenic (‑136/+32)ypfJ/purCputative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase
Reads supporting (aligned to +/- strand):  ref base C (83/83);  new base . (24/21);  total (107/105)
Fisher's exact test for biased strand distribution p-value = 7.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 213 total aligned reads displayed.

CCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCCACA  >  NC_000913_3_pae_pgi/2596724‑2597020
                                                                                                                                                     |                                                                                                                                                    
ccTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGc                                                                                                                                                      <  2:358361/150‑1 (MQ=255)
    gCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGc                                                                                                                                                  <  2:928320/150‑1 (MQ=255)
      cAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑g                                                                                                                                               <  4:164661/150‑1 (MQ=255)
        aCGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCa                                                                                                                                             <  3:309922/150‑1 (MQ=255)
         cGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCAc                                                                                                                                            <  3:120291/150‑1 (MQ=255)
         cGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCAc                                                                                                                                            >  2:2401472/1‑150 (MQ=255)
         cGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCAc                                                                                                                                            <  1:608600/150‑1 (MQ=255)
             tAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTG‑CAGCTGGCACGGCa                                                                                                                                        <  3:1098622/150‑1 (MQ=255)
              aGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTG‑CAGCTGGCACGGCaa                                                                                                                                       <  1:1385824/150‑1 (MQ=255)
               gATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTG‑CAGCTGGCACGGCAAg                                                                                                                                      <  4:1366706/150‑1 (MQ=255)
                 tACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGCAAAGCGGGATGAAATTGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGTGCGGTTGTCTTGCCGTGCCAGCT‑GCTCGTCAAgtt                                                                                                                                    <  2:958142/150‑3 (MQ=255)
                    cTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACgg                                                                                                                                          >  4:706849/1‑141 (MQ=255)
                    cTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACgg                                                                                                                                          <  3:706849/141‑1 (MQ=255)
                    cTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAgatgat                                                                                                                                 <  1:803366/150‑1 (MQ=255)
                     tCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTG‑CAGCT‑gg                                                                                                                                              >  3:1043945/1‑134 (MQ=255)
                     tCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTG‑CAGCT‑gg                                                                                                                                              <  4:104021/135‑2 (MQ=255)
                      caacaaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTG‑CAGCTGGCACGGCAAgacaaccg                                                                                                                               <  1:1423062/150‑7 (MQ=255)
                         caaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACa                                                                                                                            <  4:1143914/150‑1 (MQ=255)
                          aaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGAGGGCTGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCCCGGCAAGATGATGAACAg                                                                                                                           >  2:2040472/1‑150 (MQ=255)
                            tatatTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGaa                                                                                                                         >  1:2016411/1‑150 (MQ=255)
                              tatTCAGTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAAGAAAGCGGAATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTTCCGTGCCAGCT‑GCTCGGCAAGAAAATGAACAGaaaa                                                                                                                       <  4:1060756/150‑1 (MQ=255)
                                ttCATTATAGAAATGGTGTTAGGTGGTTCAGGGGAAGATTACGACATGAAAGCGGGTTGTAATGGCGGAGGAAGGAGCGTGCAATGCAGAGATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAGCAGAAAAAt                                                                                                                     <  2:1977361/150‑1 (MQ=255)
                                ttCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAAt                                                                                                                     <  2:220521/150‑1 (MQ=255)
                                   aTTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAgatgat                                                                                                                                 >  2:1956057/1‑135 (MQ=255)
                                    ttATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCaa                                                                                                                                       <  4:297537/128‑1 (MQ=255)
                                       taGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCa                                                                                                                   >  1:101039/1‑145 (MQ=255)
                                       taGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCa                                                                                                              >  1:2450245/1‑150 (MQ=255)
                                            agaaaggTAGGTGGTACAGAGGAAGATAAAAAAAGGAAAGCGGGATGAAATGGCGAAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑ACACGGCAAAAAAAAAAAAAAAAAAATCAGTCCAGCTGt                                                                                                         <  2:1908531/144‑1 (MQ=255)
                                                   tAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGt                                                                                                                 <  2:1016900/135‑1 (MQ=255)
                                                           tCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGcggcgg                                                                                          <  3:1037280/150‑1 (MQ=255)
                                                            cAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCggcggg                                                                                         >  3:1264165/1‑150 (MQ=255)
                                                            cAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCggcggg                                                                                         <  3:1478848/150‑1 (MQ=255)
                                                             agagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCggcgggc                                                                                        >  2:2149416/1‑150 (MQ=255)
                                                                ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTG‑CAGCTGGCACGGc                                                                                                                                         >  4:415626/1‑98 (MQ=255)
                                                                 gAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTTCCGTGCCAGCT‑GCACGGCAAGATGATGATCAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACg                                                                                    <  2:1056020/150‑1 (MQ=255)
                                                                        aCCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCAt                                                                             <  4:956025/150‑1 (MQ=255)
                                                                         ccACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACAccc                                                                                                   <  2:421138/127‑1 (MQ=255)
                                                                          cacaGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTTTTGCCGTGTCAGCT‑GGACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACTCCCAGGCGGCGGGCGACGGCTTCATAg                                                                           <  1:725213/150‑1 (MQ=255)
                                                                          cacaGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAg                                                                           >  2:1544386/1‑150 (MQ=255)
                                                                           acaGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAAt                                                                                                                     <  4:772130/107‑1 (MQ=255)
                                                                           acaGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAAt                                                                                                                     >  3:772130/1‑107 (MQ=255)
                                                                           acaGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAgg                                                                          >  4:1227864/1‑150 (MQ=255)
                                                                             aGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAg                                                                                                 <  4:266931/125‑1 (MQ=255)
                                                                             aGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAg                                                                                                 >  3:266931/1‑125 (MQ=255)
                                                                              ggAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGc                                                                                                                                         <  1:1045339/84‑1 (MQ=255)
                                                                              ggAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCtt                                                                       <  4:1264165/150‑1 (MQ=255)
                                                                                    cGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCa                                                                 <  1:213454/150‑1 (MQ=255)
                                                                                      ggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAgatg                                                                                                                                   <  1:899380/82‑1 (MQ=255)
                                                                                       gATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAgatgat                                                                                                                                 >  2:326403/1‑83 (MQ=255)
                                                                                       gATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGc                                                              >  3:442856/1‑150 (MQ=255)
                                                                                        aTGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGcc                                                             >  3:1124222/1‑150 (MQ=255)
                                                                                          gAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCAc                                                           >  1:2022755/1‑150 (MQ=255)
                                                                                               ggCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTATCTTGCCGTG‑CAGCTGGCACGGCaa                                                                                                                                       >  4:245463/1‑69 (MQ=25)
                                                                                                 cGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAga                                                                                                                                     >  1:298007/1‑69 (MQ=255)
                                                                                                   gaggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACgg                                                                                   <  2:579210/117‑1 (MQ=255)
                                                                                                    aggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTg                                                 >  3:1111632/1‑150 (MQ=255)
                                                                                                    aggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTG‑CAGCTGGCACGGCaa                                                                                                                                       >  4:519675/1‑64 (MQ=37)
                                                                                                    aggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTG‑CAGCTGGCACGGCaa                                                                                                                                       <  3:519675/64‑1 (MQ=37)
                                                                                                        aTGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCgg                                             <  2:2076692/150‑1 (MQ=255)
                                                                                                              gTTCAATGCATATATTCCGCCTCCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACgg                                       <  1:1887051/150‑1 (MQ=255)
                                                                                                               ttCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCa                                                            <  1:1835059/128‑1 (MQ=255)
                                                                                                               ttCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCa                                                            >  2:1835059/1‑128 (MQ=255)
                                                                                                               ttCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGt                                      >  2:2342854/1‑150 (MQ=255)
                                                                                                               ttCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCACTGGCGGAAAAggg                                      >  3:24316/1‑149 (MQ=255)
                                                                                                                 cAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCt                                    <  2:860062/150‑1 (MQ=255)
                                                                                                                    tGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTg                                 <  3:1227864/150‑1 (MQ=255)
                                                                                                                     gCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTcc                                                                                                               <  2:1803280/71‑1 (MQ=255)
                                                                                                                     gCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTcc                                                                                                               >  1:1803280/1‑71 (MQ=255)
                                                                                                                      cATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTc                               <  1:961048/150‑1 (MQ=255)
                                                                                                                       atatatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGt                                      >  1:1322476/1‑142 (MQ=255)
                                                                                                                       atatatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGt                                      <  2:1322476/142‑1 (MQ=255)
                                                                                                                        tatatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCa                                                                                                  <  2:238525/81‑1 (MQ=255)
                                                                                                                        tatatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCa                                                                                                  >  1:238525/1‑81 (MQ=255)
                                                                                                                         atatTCCGAGAGCGGTTGTCTTGCCGTG‑CAGCTGGCACGGCaa                                                                                                                                       <  4:444348/43‑1 (MQ=17)
                                                                                                                           atTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCAttt                          >  1:2175070/1‑150 (MQ=255)
                                                                                                                            ttCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATa                                                                            >  2:1399287/1‑99 (MQ=255)
                                                                                                                            ttCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCAtttt                         >  3:924590/1‑150 (MQ=255)
                                                                                                                               cGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAgg                                                               <  4:1446323/109‑1 (MQ=255)
                                                                                                                               cGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTtctc                      >  1:1098050/1‑150 (MQ=255)
                                                                                                                               cGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTtctc                      <  2:1098050/150‑1 (MQ=255)
                                                                                                                                 agagCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTAGATCAGGCAACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCa                    >  1:989958/1‑150 (MQ=255)
                                                                                                                                     cGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGt                >  2:1622306/1‑150 (MQ=255)
                                                                                                                                         tGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCt            <  1:1544386/150‑1 (MQ=255)
                                                                                                                                           tCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCttt          <  2:257678/150‑1 (MQ=255)
                                                                                                                                            cTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAgg                                                                                                <  1:526501/63‑1 (MQ=255)
                                                                                                                                            cTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTg         >  1:1591664/1‑150 (MQ=255)
                                                                                                                                            cTTGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGc                                                                         <  1:195350/86‑1 (MQ=38)
                                                                                                                                            cTTGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAgg                                                               >  1:263007/1‑96 (MQ=39)
                                                                                                                                            cTTGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAgg                                                               <  2:263007/96‑1 (MQ=39)
                                                                                                                                             ttGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGc                                               <  1:343088/111‑1 (MQ=255)
                                                                                                                                              tGCCGTG‑CAGCTGGCACGGCAAGATGATGAACGGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTc       >  3:471293/1‑150 (MQ=255)
                                                                                                                                              tGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATa                                                                            <  2:1868146/81‑1 (MQ=38)
                                                                                                                                              tGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATa                                                                            >  1:1868146/1‑81 (MQ=38)
                                                                                                                                              tGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTc       <  3:282184/150‑1 (MQ=255)
                                                                                                                                              tGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTc       >  2:1283269/1‑150 (MQ=255)
                                                                                                                                              tGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTc       <  2:786944/150‑1 (MQ=255)
                                                                                                                                              tGCCGTG‑CAGCTGGCACGGCAAGATGATGAACAGAAAAAGCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTc       >  3:1444333/1‑150 (MQ=255)
                                                                                                                                               gCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGc                                                              >  1:390651/1‑94 (MQ=255)
                                                                                                                                               gCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTcc      >  1:770846/1‑150 (MQ=255)
                                                                                                                                                  gTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCcac   >  3:550307/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCT‑GCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCCACA  >  NC_000913_3_pae_pgi/2596724‑2597020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑