| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_vch_pgi | 3,442,845 | G→A | A418V (GCT→GTT) | secY ← | preprotein translocase membrane subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_vch_pgi | 3,442,845 | 0 | G | A | 91.7% | 22.6 / NA | 12 | A418V (GCT→GTT) | secY | preprotein translocase membrane subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (5/6); minor base C (1/0); total (6/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCA > NC_000913_3_vch_pgi/3442715‑3442989 | aCGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAACCATAAAGTCCATAATCa < 2:184405/149‑1 (MQ=255) tCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAACCATAAAGTCCATAATCACGACAACAACGATAAg > 1:90109/1‑149 (MQ=255) cGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAACCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCAc < 1:20046/149‑1 (MQ=255) ggCGAGCAATTAGCGGGGGTAGCGTTTTAGGTTCGCCTTCTTCAAGGCAGACTCGTACTGACGGGACATGTGCAGAGTTGGGTGTTGGACCGTGAAGTCCATGATCGCGACAACAACGATGAGCAGTGAGGTCCCACCGAAGTAGAACg < 2:142709/149‑1 (MQ=255) cAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAACCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACtt > 2:106689/1‑149 (MQ=255) ccTTTCACGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAACCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCAt < 1:475/149‑1 (MQ=255) tttCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAACCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGa > 1:24682/1‑149 (MQ=255) cAGAGTCATACGGACGGGAGATCATCAGAGTTTGTACTTGAACCATAAAGTCCACAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAg < 2:220793/149‑1 (MQ=255) ggACATCATCAGAGTTTGCACTTGACCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCCCCGCAGTAGAACGGTACTTTCATTGCATCCCGCCTGAACTCCGGGCACAGGCAGATAAAGGTAATACACAGCCCac > 2:48254/1‑149 (MQ=255) catcaGAGTTTGCACTTGAACCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCaccaacca < 2:90109/149‑1 (MQ=255) cTTGAACCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATAAAGGGAACCAACCCGGGTCAGGCGGGTc > 2:38682/1‑149 (MQ=255) ttGAACCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCAACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGCCAGATAAAGGTAATATACAACGAACCAACAAGGGTCAGGCGGGGCa > 2:54713/1‑149 (MQ=255) | ACGGAAGCACGAACTTTCATTTTTACTCTCCGTAACTTCTCGGGCGACCAATTATCGGCCGTAGCCTTTCAGGTTCGCCTTCTTCAATGCAGACTCATACTGACTGGACATCATCAGAGTTTGCACTTGAGCCATAAAGTCCATAATCACGACAACAACGATAAGCAGTGAGGTCCCACCGAAGTAGAACGGTACTTTCATTGCATCACGCATGAACTCCGGGATCAGGCAGATAAAGGTAATATACAGCGCACCAACCAGGGTCAGGCGGGTCA > NC_000913_3_vch_pgi/3442715‑3442989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |