New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi = 400196212 (0.620)3 (0.160) 3/282 2.5 17.3% coding (537/951 nt) corA magnesium/nickel/cobalt transporter
?NC_000913_3_vch_pgi = 4001989 17 (0.910)coding (564/951 nt) corA magnesium/nickel/cobalt transporter
Rejected: Frequency below/above cutoff threshold.

GTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCAACTGGAGCAGGCGCGTGAAATCCTGCGAGATATCGAATCCCTGCTGCC  >  NC_000913_3_vch_pgi/4001833‑4002108
                                                                                                                                 |                                                                                                                                                  
gTTCGAAACCAAAAGCGAACGGTTGGCAGTTGAAATTGAAAATTTCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGGACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCt                                                                                                                                 <  1:124478/149‑1 (MQ=255)
 ttCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGAGTATGACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTg                                                                                                                                >  1:235061/1‑149 (MQ=255)
           aaaTCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTcgc                                                                                                                      <  2:232723‑M2/149‑4 (MQ=255)
              tCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCCACTCTGGCGaactttccagccgatatcttccagttccgcca                                                                                                                    >  1:146099‑M1/1‑116 (MQ=255)
              tCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCCACTCTGGCGaactttccagccgatatcttccagttccgcca                                                                                                                    <  2:146099‑M1/148‑33 (MQ=255)
                  aCAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGATGGAAAGTTagccaatgtc                                                                                                               <  1:100800‑M2/149‑11 (MQ=255)
                    aGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTg                                                                                                             >  1:197462/1‑149 (MQ=255)
                            gATCAAATCGAAAATATCTATAGCGACCTGGAGTAGTCGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGGGTACGTCGGGGCGCTTTCCACTCTGGCGGATCTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATAc                                                                                                     <  2:96245/149‑1 (MQ=255)
                              tGAAATTGAAAATATCTATAGCGATCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCACCCCTGGCTGAATTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATAccc                                                                                                   <  2:226824/149‑1 (MQ=255)
                                                      ccTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGc                                                                           <  1:232723/149‑1 (MQ=255)
                                                                ttGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGcgcg                                                                 >  2:289069/1‑149 (MQ=255)
                                                                     ccGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTAc                                                            >  2:99110/1‑149 (MQ=255)
                                                                               aTGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCa                                                  >  2:10765/1‑149 (MQ=255)
                                                                                        cATCAGGGCGATGAGTACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCCGTGTCTGATGGATACCCAGCGCGCGCTGAACTTCCTGCTGCGTAAAGCGCGTTCACCGGGCGGCCCACTgcacca                                         >  2:130438/1‑144 (MQ=255)
                                                                                                   tGAGTACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTcgc                                                                                                                      >  2:103144‑M2/1‑58 (MQ=255)
                                                                                                   tGAGTACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTcgc                                                                                                                      <  1:103144‑M2/61‑4 (MQ=255)
                                                                                                       gacgacgAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCAACTGGAGCAGGCGCGTGAAATCCt                          <  2:301500/148‑1 (MQ=255)
                                                                                                        acgacgAGGCGCTCTCCCCTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCAACTGGAGCAGGCGCGTGAAATCCTg                         <  1:267157/149‑1 (MQ=255)
                                                                                                                        gacattggctaactttccatccgatatcttccagttccgccagagtggagagcgcctcgtcgtactcatcgccctgatgcccttccataatcacccggctcaactgctccaggtcgctatagatattttcaatttcatctgccaactgt         <  1:100800‑M1/144‑140 (MQ=255)
                                                                                                                             tGGCGGAACTGGAAGATATCGGCTGGAAAGTTcgccagagtggagagcgcctcgtcgtactcatcgccctgatgcccttccataatcacccggctcaactgctccaggtcgctatagatattttcaatttcatctgccaactgttcga     <  2:146099‑M2/148‑117 (MQ=255)
                                                                                                                             tGGCGGAACTGGAAGATATCGGCTGGAAAGTTcgccagagtggagagcgcctcgtcgtactcatcgccctgatgcccttccataatcacccggctcaactgctccaggtcgctatagatattttcaatttcatctgccaactgttcga     >  1:146099‑M2/1‑32 (MQ=255)
                                                                                                                               gCGaactttccagccgatatcttccagttccgccagagtggagagcgcctcgtcgtactcatcgccctgatgcccttccataatcacccggctcaactgctccaggtcgctatagatattttcaatttcatctgccaactgttcgattt  <  2:232723‑M1/149‑147 (MQ=255)
                                                                                                                               gCGaactttccagccgatatcttccagttccgccagagtggagagcgcctcgtcgtactca                                                                                          >  2:103144‑M1/1‑3 (MQ=255)
                                                                                                                               gCGaactttccagccgatatcttccagttccgccagagtggagagcgcctcgtcgtactca                                                                                          <  1:103144‑M1/61‑59 (MQ=255)
                                                                                                                                 |                                                                                                                                                  
GTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAAAATATCTATAGCGACCTGGAGCAGTTGAGCCGGGTGATTATGGAAGGGCATCAGGGCGATGAGTACGACGAGGCGCTCTCCACTCTGGCGGAACTGGAAGATATCGGCTGGAAAGTTCGCCTGTGTCTGATGGATACCCAGCGCGCGCTCAACTTCCTGGTGCGTAAAGCGCGTTTACCGGGTGGGCAACTGGAGCAGGCGCGTGAAATCCTGCGAGATATCGAATCCCTGCTGCC  >  NC_000913_3_vch_pgi/4001833‑4002108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.