New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi 2505963 =27 (1.400)3 (0.160) 3/278 2.5 11.1% coding (670/1086 nt) ypdF Xaa‑Pro aminopeptidase
?NC_000913_3_vch_pgi 2505985 = 22 (1.190)coding (648/1086 nt) ypdF Xaa‑Pro aminopeptidase
Rejected: Frequency below/above cutoff threshold.

AGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTC  >  NC_000913_3_vch_pgi/2505837‑2506131
                                                                                                                                                    |                                                                                                                                                  
aGCGCACGCCGGGGCGAATCGCGGAGGTTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTGAACGGCAGGTGAGATTGGGGGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCGGAGCAGTAGCCCTGATAc                                                                                                                                                    >  2:259438/1‑149 (MQ=255)
                  tCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGAAAGGTGCGCGTCATATCAGAGCAGTCGCCCTGCTACAGCGCACCGAAATCgaga                                                                                                                                  >  1:147328/1‑149 (MQ=255)
                      ggAGATGGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGa                                                                                                                              <  2:55754/149‑1 (MQ=255)
                             gctgctGGCTTTGCCTGCCGGACAATTTGATCGGCGTTAATCAGCCGGTTACATTCGGCGCTCAGCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTc                                                                                                                       <  2:273911/149‑1 (MQ=255)
                               tgcGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAg                                                                                                                                                  <  1:63585/120‑1 (MQ=255)
                               tgcGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAg                                                                                                                                                  >  2:63585/1‑120 (MQ=255)
                               tgcGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGc                                                                                                                     >  2:265373/1‑149 (MQ=255)
                                      tgtgCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGAAGTAGCCCTGATACAGCCCACCGAAATCGAGAGTGACAAACTCCCCCGCTGc                                                                                                              >  2:149474/1‑149 (MQ=255)
                                            tGCAGGACGATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAAt                                                                                                        <  1:118773/149‑1 (MQ=255)
                                             gCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATc                                                                                                       <  1:282836/149‑1 (MQ=255)
                                             gCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATc                                                                                                       <  1:282876/149‑1 (MQ=255)
                                                ggACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGTGGGAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTg                                                                                                    >  1:334445/1‑149 (MQ=255)
                                                     aTTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACCCGCCCGCTGCAACAGTCTTGTCCCt                                                                                               >  2:79748/1‑149 (MQ=255)
                                                                 ttAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTg                                                                                                               <  1:298626/121‑1 (MQ=255)
                                                                 ttAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTg                                                                                                               >  2:298626/1‑121 (MQ=255)
                                                                       agcagGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAg                                                                             >  1:221172/1‑149 (MQ=255)
                                                                              gagaTTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCa                                                                      <  1:268561/149‑1 (MQ=255)
                                                                                         agcgggcgagtttgtcactctcgatttcggtgcgctgTATCAGAGCAGTAGCCCTGATACa                                                                                                                                                   <  1:99120‑M1/24‑1 (MQ=255)
                                                                                         agcgggcgagtttgtcactctcgatttcggtgcgctgTATCAGAGCAGTAGCCCTGATACa                                                                                                                                                   >  2:99120‑M1/38‑61 (MQ=255)
                                                                                          tCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTg                                                          >  1:315/1‑149 (MQ=255)
                                                                                               ccTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCCGATACAGCGCACCGCGAACCAGACTGACAAACTCGCCCGCTGCAACAATCTTTTCACTAGCTTTGCCCTACGGCAGCGCCCCACGCCAGCCACTGGCGAc                                                     >  2:165961/1‑149 (MQ=255)
                                                                                                cTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCCCAGAATTCCAGGGTGACAAACTCGCCCGATGCAAAAATCTTACCGCAGGCTTTGCCGTGCGCCCGCTCCCCGCGCCCGCCCCTGGCcaca                                                    >  2:237076/1‑149 (MQ=255)
                                                                                                                            tgtatcagggctactgctctgataCAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCt                                                                                                                >  2:99120‑M2/25‑61 (MQ=255)
                                                                                                                            tgtatcagggctactgctctgataCAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCt                                                                                                                <  1:99120‑M2/37‑1 (MQ=255)
                                                                                                                              tatcagggctactgctctgataCAGCGCAACGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGTGccc                      >  1:288093‑M2/23‑149 (MQ=255)
                                                                                                                                  agagCAGTCGCCCTGATACAGCGCAACGAGTAAGATTGGGAGAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCCGCAGCGCCACACGCCAGCCACTGGCGCGAATGGTGTCAAAAGGGGCTTTTTCTGCTCCctgct                  >  2:244255/1‑149 (MQ=255)
                                                                                                                                  AGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCctgct                  >  1:265373‑M1/1‑149 (MQ=255)
                                                                                                                                             ccTGATACAGCGCACCGTAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGACGCGTTTTCTCCGCCCTGCTGGCGCTTAAAc       <  1:331019/149‑1 (MQ=255)
                                                                                                                                                  taCAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTc  <  2:171566‑M2/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGCGCACGCCGGGGCGAATCGCGGAGATTGCTGCGAGCTGTGCCTGCAGGACAATTTGATAGACGTTAAACAGCAGGTGAGATTCGGCGCTCACCCCTTCGCCATTCACCAGCAAGGTGCGCGTCATATCAGAGCAGTAGCCCTGATACAGCGCACCGAAATCGAGAGTGACAAACTCGCCCGCTGCAACAATCTTGTCGCTGGCTTTGCCGTGCGGCAGCGCCCCACGCCAGCCACTGGCGACAATGGTGTCAAAAGAGGCTTTTTCTGCGCCCTGCTGGCGCATAAACCACTC  >  NC_000913_3_vch_pgi/2505837‑2506131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.