Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_pgi526,8000AG40.0% 28.9 / 20.8 36E1180E (GAA→GAG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (6/15);  new base G (11/3);  total (17/19)
Fisher's exact test for biased strand distribution p-value = 5.93e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATATTA  >  NC_000913_3_vch_pgi/526660‑526948
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gggCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatgataa                                                                                                                                              >  2:990/1‑147 (MQ=255)
    tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                                          <  2:137982/149‑1 (MQ=255)
    tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCCAAGCCGGCAATACGACGTGGGGCGGGGAGTCCGATAAATgg                                                                                                                                          >  2:150493/1‑149 (MQ=11)
    tGACGGTGGACCAACGGGCCAGACAGGTGGAGCCGGAATGCACACCGGCGCGAACAGCTCGTCTTTATCGCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAACACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgg                                                                                                                                          <  1:338426/149‑1 (MQ=17)
     gACGGTGGAGCAACTGGCCGGACAGGTGGGGCCGGAATACACATCGGCGCGAAAAGCTCATATTTATCACTGCGACCCCCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGCGGAATATGATGAATggg                                                                                                                                         <  2:345661/149‑1 (MQ=14)
         gTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCaa                                                                                                                                     >  1:343195/1‑149 (MQ=255)
             aGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAg                                                                                                                                 <  2:347984/149‑1 (MQ=255)
             aGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCGTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAg                                                                                                                                 <  1:208871/149‑1 (MQ=255)
              gCAACGGGCCAGACAGGTGGAGCCGGAATACACCCCGGCGCGAAAAGCTCCTCTATATCACTGCGACCAGCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGc                                                                                                                                <  2:389337/149‑1 (MQ=17)
                 aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATACGCCAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCtga                                                                                                                             >  1:90691/1‑147 (MQ=11)
                   tGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                                           >  1:87150/1‑149 (MQ=255)
                     gcacaaCAGGTGGAGCCGGAATCCACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGCAGACGGCAATACGGCGTGGCGGGGAGAGAGTCATGAAGGGGGCAACCAGCTTAATga                                                                                                                         >  2:374887/6‑149 (MQ=11)
                     gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                         <  1:990/149‑1 (MQ=255)
                        aggcAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATTTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                      <  2:381914/146‑1 (MQ=255)
                            cggTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCGATACGGCTTGGCGCCGGTATTTTTATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                                  <  1:290850/148‑1 (MQ=11)
                             ggTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATAGGGCAACAAGCTTAATGAGGAGAAcc                                                                                                                 >  2:54786/1‑149 (MQ=11)
                              gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCACCGAAGACGGCAATACGGCGTGGCGGGGGGAGTCGGAAGAATGGGGCAACCAGCCTAATGTGCAGAAccc                                                                                                                >  2:156022/1‑149 (MQ=11)
                               tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCg                                                                                                               >  1:362294/1‑149 (MQ=255)
                                ggAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGTGGACTGCCGCTGGCGCTTATCAGCGCAGACGGCAATACAGCCTCGCGCGTGGTAAATTCTGAATAGGACAACCAGCTTCGTTAGGAGAAccctc                                                                                                              >  2:45656/1‑147 (MQ=14)
                                   gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGGACCCGcatc                                                                                                           >  1:312935/1‑149 (MQ=11)
                                   gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGAGGACTGCCGCTGGCGCTTATCAGCGAAGACGCCAATACGGCGTGGCGCGGGGAGAATGATGAATGGCGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                           >  1:159601/1‑149 (MQ=11)
                                       gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                      <  2:330173/134‑1 (MQ=255)
                                       gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                      >  1:330173/1‑134 (MQ=255)
                                       gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCGTGTCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacct                                                                                                       >  1:308247/1‑146 (MQ=11)
                                             acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCa                                                                                                 >  1:140545/1‑149 (MQ=255)
                                                   gcgcGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGCAGACGGCTATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGGGGACCCGCATCATGTGTATCAGCCGTa                                                                                           >  1:74763/1‑149 (MQ=255)
                                                    cgcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAc                                                                                          <  1:375834/149‑1 (MQ=255)
                                                              tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                                <  1:320609/149‑1 (MQ=255)
                                                               cATCTTTGTCACTGCGACCCCCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCTGCATCATGTGTATCAGCCGTACCGTCTGCCAgg                                                                               <  1:36413/149‑1 (MQ=255)
                                                                            gcgcCCACCGGTGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGACAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                                                                  <  1:358659/149‑1 (MQ=255)
                                                                                      gggACTGCCGCTGGGGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAg                                                        <  1:305948/149‑1 (MQ=255)
                                                                                            gccgccCGCCCTAATCCGCGAAGACGGCCAGACGGCCTGGGGCCCGTAATAAAATGAATTGCGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTACCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGt                                                  <  2:336621/149‑1 (MQ=14)
                                                                                             ccGCTGGCGCTTATCAGCGAAGACGACAATACGGCGTGGAGCGCGGGATATGATGAATGGGGCAACCGGCTTAATGAGGAGAACACGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGGAGCATGATGAGGAATAAGCGCTGTa                                                 >  1:354111/1‑149 (MQ=17)
                                                                                              cGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTAc                                                <  1:63822/149‑1 (MQ=255)
                                                                                                  ggCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACtata                                            <  1:198504/149‑1 (MQ=255)
                                                                                                    cgcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAc                                          <  1:213663/149‑1 (MQ=255)
                                                                                                                                            atatGCTGAATTGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATATTa  <  1:24773/149‑1 (MQ=255)
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GGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTATATTA  >  NC_000913_3_vch_pgi/526660‑526948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑