New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi = 417180322 (1.000)4 (0.190)
+TTGATGT
4/276 2.5 16.7% intergenic (+47/‑254) rrfB/murB 5S ribosomal RNA of rrnB operon/UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding
?NC_000913_3_vch_pgi = 4213155 20 (0.910)noncoding (116/120 nt) rrfE 5S ribosomal RNA of rrnE operon
Rejected: Frequency below/above cutoff threshold.

GCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAA  >  NC_000913_3_vch_pgi/4213013‑4213290
                                                                                                                                              |                                                                                                                                       
gCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGACGGTAGAGTGGGGTCTCCCCATGCGAGCGAAGGGAACTACCAGccatca                                                                                                                                   <  2:223455‑M2/149‑7 (MQ=255)
      gctgTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAg                                                                                                                             <  1:119359/146‑1 (MQ=17)
          tCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGaaaa                                                                                                                         >  1:310256/1‑149 (MQ=35)
          tCTGATAAAACAGAATTTGCCTGGCGGCAGTAAAGTTTTGAGCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGCTGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGaaaa                                                                                                                         >  1:384615/1‑149 (MQ=11)
            ggaTAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGacatcaaataaaacaaaa                                                                                                                       >  2:256867‑M2/2‑131 (MQ=255)
                   aCAGGATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGCCTCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTc                                                                                                                <  2:343191/149‑1 (MQ=255)
                     ggactttGCCTGGCGGCAGCAGTGCGGTGGTCCCACCTGACCCCATACCGAATTCAGAAGTGAAACGCCGTATCGCCTATGGTAGTGTGTGGTCTCCCCATGCGAGTGTAGGGAACTGCCAGTCATGAAATTAGAAAACCCCCGGTCCa                                                                                                              <  2:242626/145‑1 (MQ=14)
                                          gcgcGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGgcatcaaataaaacgaaaggctcagtctttcgactgagcctttcgttt                                                                                         >  1:324995‑M2/1‑101 (MQ=255)
                                                    cccACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTACCGCCGATGGTAGTGAGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAAAAAAACCCAGGTCACAAAGGCCGGGGTTTTTTGCATATCAatcaat                                                                               >  2:185107/1‑145 (MQ=17)
                                                      cACCTGACCCCATGCCGAACTCAGAAGTGAAACGACGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTGGAAAGACCCCGGTCCATAAGGCCGGGGTTTTTTGCGTATCAATTTTTTg                                                                             >  2:308419/1‑149 (MQ=18)
                                                        ccTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCa                                                                           <  1:198775/149‑1 (MQ=255)
                                                               ccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCat                                                                                         <  1:230182/128‑1 (MQ=255)
                                                               ccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCat                                                                                         >  2:230182/1‑128 (MQ=255)
                                                               ccATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATAAATTATTTGAATGATGaa                                                                    >  1:261255/1‑149 (MQ=255)
                                                                  agcagAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGGGTGTGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAggg                                                                 <  2:110425/145‑1 (MQ=255)
                                                                                 tGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGgcatcagataaaacgaaaggcccagtctttcgactgagcctttcgt                                                                                           <  1:143174‑M2/108‑47 (MQ=255)
                                                                                 tGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGgcatcagataaaacgaaaggcccagtctttcgactgagcctttcgt                                                                                           >  2:143174‑M2/1‑62 (MQ=255)
                                                                                    aaCGCCGTAGGGTGCGTGGTAGTCGGGGGTCTCCCCAGGCGAGAGTAGGGAACTGCCAGGAATCAAATTAGAAAATCCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTg                                               <  2:382433/149‑1 (MQ=37)
                                                                                                    tGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTAAGTTCTGTATATCAAAATtcgtc                               >  1:386952/1‑149 (MQ=255)
                                                                                                             ggggTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCaaa                      >  1:47640/1‑149 (MQ=255)
                                                                                                             ggggTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCaaa                      <  1:342339/149‑1 (MQ=255)
                                                                                                                       cATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTACAAAAGGCCGGGGTTTTTTGCATATCAATTATTTGCCTGATAAAGGGACTCTCATGTGAGTTCTTTATATCCAAATGCATCGCAGTCAAATTACtttggg            >  1:43240/1‑144 (MQ=37)
                                                                                                                              gagTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGCATCTCATGTCAGTTCTGTATATCCAACTCCGACCTATTCAAATTACTGGTGCCGATcgt     >  2:33190/1‑147 (MQ=255)
                                                                                                                                gTAGGGAACTGCGAGGCATCGAATTAGAAAACCCCCGGTCCATAAGGCCGGTGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGa   <  2:285811/149‑1 (MQ=255)
                                                                                                                                 tAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATACGCCAGGGGTTTTTTGCATATCAGTTATTTGCATGATGAAGGGAATCTCAGGTCAGGTCTGTATATCCAAATTCGTAGTATTGTAATTCCTATTAGCGATCTTgta  >  2:57063/1‑147 (MQ=255)
                                                                                                                                              |                                                                                                                                       
GCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAA  >  NC_000913_3_vch_pgi/4213013‑4213290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.