Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_vch_pgi526,7920AC34.1% 32.2 / 38.5 45S1178R (AGC→CGC) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (7/22);  new base C (3/12);  total (11/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.90e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTA  >  NC_000913_3_vch_pgi/526649‑526922
                                                                                                                                                |                                                                                                                                  
ttGCGGAGTGCAGGGGGACGGGGGAGTCACTGGCCAGACAGGTGGAGCCGGAATACTCACCGGCGCGAAAAGC‑TCATCTTTATAACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGcgcggg                                                                                                                               <  2:31985/149‑3 (MQ=11)
     cAGGGCGGGCTGACGGTGGAGCAACTGGCCAGCCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAata                                                                                                                          <  1:77837/149‑1 (MQ=255)
      aGTGCGGGCTGACGTTGGCTCAACTGGCCAGACTGGTGGAGTCGTAATACACTCCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAatat                                                                                                                         <  1:177261/149‑1 (MQ=17)
        tGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGCGCCGGAATACACACCGGCGCGAAAAAC‑TAATATTTATAAATGAGACCACCGGGGACTGACGCTGGCGCTTATCAGCGAAAACGGCAATACGGAGTGGGGCGGGGGGTatga                                                                                                                       >  1:20153/1‑149 (MQ=11)
        tGCGGGCGGAGGGTGGAGCAGTGGGCCAGACAGGTGGCGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatga                                                                                                                       <  2:328561/149‑1 (MQ=17)
         gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                      <  2:117256/149‑1 (MQ=255)
         gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                      <  1:268763/149‑1 (MQ=255)
            ggCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGaa                                                                                                                   <  2:197317/149‑1 (MQ=255)
             gCTGACGGTGGGGCAACTGGCCAGACAGGTGGAGCAGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAAt                                                                                                                  <  1:56699/149‑1 (MQ=11)
               tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgg                                                                                                                <  1:159478/149‑1 (MQ=255)
               tGACGGGGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                <  1:7495/149‑1 (MQ=255)
                gccGGTGGAGTAACGGGCCAGACAGGTGGAGGCGGAATACACACCGGTGCGGAAAGC‑TCATCTTTATCACTGGGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                               <  2:361811/147‑1 (MQ=11)
                gccGGTGGAGCAACTGGCCATACAGTTGGATCCGGAATACCCACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                               <  1:43727/147‑1 (MQ=11)
                gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATGCACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                               <  1:301842/149‑1 (MQ=255)
                gACGGCGGAGCAACTGGCCAGACCGGTGGAGCCGGCATGCAC‑CCCGAGAAACCGCCTTCACCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGGGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                               <  1:108561/149‑1 (MQ=14)
                     tGGAGTAACGGGCCATACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGATCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAc                                                                                                          <  1:83628/149‑1 (MQ=11)
                                gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATga                                                                                               <  1:236183/149‑1 (MQ=255)
                                  cAGACCGGTGGAGCCGGACTACACACCGGCGCGAAAAGC‑TCATCTTTATCGCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAAGACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagt                                                                                             <  1:203361/149‑2 (MQ=11)
                                   agagagGTGGACGCGGAATACCCACAGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                                            <  2:210619/149‑1 (MQ=17)
                                   aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGTCGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                                            <  1:151219/149‑1 (MQ=255)
                                     aCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGgaga                                                                                          >  1:330040/1‑149 (MQ=255)
                                      cAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGaa                                                                                         >  1:110628/1‑149 (MQ=255)
                                         gTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatg                                                                                                                        >  1:168095/1‑115 (MQ=255)
                                         gTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatg                                                                                                                        <  2:168095/115‑1 (MQ=11)
                                         gTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGAGCGCACCGGGGACGGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                      <  1:303678/149‑1 (MQ=255)
                                          tGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATAGGATGAATGGGGCAACCAGCTTAATGAGGAGGACCCg                                                                                     >  1:666/1‑149 (MQ=255)
                                            gAGCGGGACTACACACCGGCGCGAAAAGC‑TCCTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCCTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGAAGTATTATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGca                                                                                   <  2:162855/149‑1 (MQ=11)
                                              gCCGGAATACAGACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                 >  1:237225/1‑149 (MQ=255)
                                               cctgAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatca                                                                                <  1:273951/146‑1 (MQ=255)
                                                 ggAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtg                                                                              <  2:242039/149‑1 (MQ=255)
                                                      agaacccGGCGCGAAAAGG‑TCATTTTTATTACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTAt                                                                         <  2:378743/144‑1 (MQ=255)
                                                       cacacCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATc                                                                        <  1:85781/149‑1 (MQ=255)
                                                                            aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGGGGAATATGATGAATGGGGCGACCAGCTTAATGAGGAGAACCCGCATCATGTGTGTCGGGCGTGACCTCTACCAggg                                                    >  1:35646/1‑149 (MQ=17)
                                                                              cTTTATCACTGCGACCCCCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgca                                                  <  1:162881/149‑1 (MQ=255)
                                                                                 tATCACTGCGACCACCGGGGACTGCCGCGGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagca                                               <  1:23786/149‑1 (MQ=255)
                                                                                   tCACTGCGACCACCGGGGACTGCCGCCGGCGCTTATCCGAGAAGACGGCAATACAGCGTGGAGCGCGGACTATGATGAATGGGGCAACCGGCTAACAGCGGAGAACCCGCATCATGTGCGTCAGCCGTACCGACTGCCAGGgcagcgcc                                             >  2:203006/1‑146 (MQ=255)
                                                                                        gCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGGACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                                        <  1:318290/149‑1 (MQ=255)
                                                                                             caccGGGGACTGCCGCTGGCGCTTATAAGCGAAGATAGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGa                                   >  1:384860/1‑149 (MQ=255)
                                                                                                     aCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGc                           <  1:324170/149‑1 (MQ=255)
                                                                                                         ccGCTGGAGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                       <  1:183052/149‑1 (MQ=255)
                                                                                                               gcgcTCATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATaa                 <  1:27948/149‑1 (MQ=255)
                                                                                                               gcgcTAATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGCATATGCTGAATGGGGCAACCAGCATAACGCGCATAACACGCATCATGTGGATCTGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATaa                 <  1:286575/149‑1 (MQ=17)
                                                                                                                 gcTTATCCGCGAAGAAGGCAATCCGCCACGAAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCTAGATTAGGAATCAGGGCTGTACTATAAcc               >  2:177234/1‑149 (MQ=17)
                                                                                                                              gACGGCAATACGGCGTGGACCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGtacta  <  1:199532/149‑1 (MQ=255)
                                                                                                                                 ggCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTa                                                                                                   <  1:212391/49‑1 (MQ=255)
                                                                                                                                 ggCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTa                                                                                                   >  2:212391/1‑49 (MQ=255)
                                                                                                                                                |                                                                                                                                  
TTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGC‑TCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTA  >  NC_000913_3_vch_pgi/526649‑526922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑