New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	3867586 =	35 (0.820)	4 (0.100)	4/274	5.8	9.9%	intergenic (‑164/‑142)	ibpA/yidQ	heat shock chaperone/DUF1375 family outer membrane protein
	?	NC_000913_3_hsa_tpiA	3867601 =	39 (0.960)					intergenic (‑179/‑127)	ibpA/yidQ	heat shock chaperone/DUF1375 family outer membrane protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGATCAACAGATGAATTTATTATGATAA  >  NC_000913_3_hsa_tpiA/3867443‑3867734                                                                                                                                                |                                                                                                                                                     aGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTAt                                                                                                                                                 >  1:498574/1‑149 (MQ=255)          gTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCATCATCGGCTACGATGTAGAAATGGGTCTGGACATGAACTTTTAAAGGCTTGTCAGCGGACTTTTTTTTTTACTTTATTGTCTATTTCacaccg                                                                                                                                        >  2:400466/1‑144 (MQ=255)                  ttCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAggggg                                                                                                                                >  1:586242/1‑149 (MQ=255)                  ttCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTAAggggg                                                                                                                                >  1:695736/1‑149 (MQ=255)                    ccACAAGGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGgca                                                                                                                              <  1:753453/149‑1 (MQ=255)                        aaTGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTACAGCTTAAGGGCCATTGGATAGACTGAAGGGacagaca                                                                                                                          >  1:725197/1‑146 (MQ=255)                         aTGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGCAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCCGTTTATGGACTATTGCATTAACTGAGCAGGCCGCACg                                                                                                                         >  2:401555/1‑149 (MQ=255)                             agagatcaggtAGCCAGAACACCCCTCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCTGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAAc                                                                                                                     <  2:95419/144‑1 (MQ=255)                                 gtcaggtAGCCAGAACACCCATCAGGCGCGTCCCCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTa                                                                                                                 <  1:9250/149‑1 (MQ=255)                                   caggtGGCCAGAACGCCCGTCAGGCGCGTCCTCATCGGTTACGAGGTAAAAATGGGTATGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTaaa                                                                                                               <  2:262701/149‑1 (MQ=255)                                   caggtAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTaaa                                                                                                               <  1:593190/149‑1 (MQ=255)                                        acacAGAACACCCAACAGGCGCTTCCCCATCGGCTACTAGATAAAAATGGGTCTGGAAACGAACTTTACAAGCCTCACCAGCAGACTTTCTCTTGCAGTTTATGGTCTACTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCg                                                                                                          <  2:220939/146‑1 (MQ=255)                                         acaaGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGa                                                                                                         <  2:267837/146‑1 (MQ=255)                                           cAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAGGGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATa                                                                                                       >  1:25548/1‑149 (MQ=255)                                             gAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATaac                                                                                                     >  2:633489/1‑147 (MQ=255)                                                 acCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAgccgcc                                                                                                 >  1:209585/1‑149 (MQ=255)                                                   ccATCCGTCGCCTCCGTATCTGCTACGAAGTAAAAATGGGTCTGGAAATGAACTTTTCAAGGCTTATGATCGGACTTTTTTTTTCAGTTTATTGTCTATTTGGTAGACTGAGGGAGCCACACGCAGCGTTAAAGTGCGATGCCTGCcac                                                                                               >  2:621306/1‑149 (MQ=255)                                                     aTCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTCATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAg                                                                                             >  2:630260/1‑149 (MQ=255)                                                          gcgcGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGaaa                                                                                                                      <  1:511870/119‑1 (MQ=255)                                                          gcgcGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGaaa                                                                                                                      >  2:511870/1‑119 (MQ=255)                                                            gcgTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACg                                                                                      >  1:183997/1‑149 (MQ=255)                                                               tCCTCATCGGCTACGATGTAAAAATGGTGCTGGAAATTAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTaa                                                                                   <  2:524830/149‑1 (MQ=255)                                                                                     aaTGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTTTAGTTTATGGTCTATTGCATAGACTGAGGGAGCAGCACGAAACGTTAAAGTACGATAGCCGCCACTGTGCGACGTCATGATCATGTTATTTGTAGCAAg                                                             >  2:597416/1‑149 (MQ=255)                                                                                             tGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTATATGCTCTATTGCATAGACTGAGGGGCCAGCACGCAACGTTAAAGTACGATAGCCGCCACAGTGCGACGCACTGATCTTGTTCTTCTCAGCAAGGGacccct                                                     >  2:708603/1‑144 (MQ=255)                                                                                               gcaaTGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATATCCAGAGGGGGCAGCACGAAACGTTAAAGTACGATAACCGCCACAGTGCGACGAAATCATGATGTTATTTTCAGCACCGGATCGCtat                                                   >  2:449747/3‑149 (MQ=255)                                                                                                 aaTGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGTAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTCCGACGTAATGATGATGTTCTATTCCGCAAGGGATCGCTATaa                                                 >  2:614343/1‑149 (MQ=255)                                                                                                    gAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACtc                                              >  1:164866/1‑149 (MQ=255)                                                                                                        ttttCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACtcatca                                          <  1:373113/149‑1 (MQ=255)                                                                                                              aGCCTTATCAGCGGACTTTTTTTTGCAGTTTAGGGTCTATTGCATAGACGGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCaa                                    <  2:58369/149‑1 (MQ=255)                                                                                                              aGCCCTAGCAGCGGCCTTTTTTTTGCAGTTTATGGCCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCaa                                    <  1:549898/149‑1 (MQ=255)                                                                                                                        gCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGAt                          >  1:259516/1‑149 (MQ=255)                                                                                                                             cTTTTTTTTGCAGTTTATGGTCTAGTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGATCAACa                     <  2:498574/149‑1 (MQ=255)                                                                                                                              ttttttttGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGCTCAACAg                    >  1:59582/1‑149 (MQ=255)                                                                                                                              ttttttttGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGATCAACAg                    >  1:574757/1‑149 (MQ=255)                                                                                                                                         gTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGATCAACAGATGAATttatt         <  1:616644/149‑1 (MQ=255)                                                                                                                                          gtgatagTCTATTGCATAGACAGAGCCGGCAGCACGAACGGGCAAAGTGGGATAGCCGCCACAGATCGACGGAATGAGGATGTTATTTTCAGCAAGGGACAGCTATAACTCATAAAGCCCACACCCACGATCAGCAGATGAATttagta        >  2:64173/7‑149 (MQ=255)                                                                                                                                                gTCTAGTGCATAGCCTGAGGGGGCAGAACTAAACGTTAAAGGGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGATCAACAGATGAATTTATTATGATaa  <  2:594914/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     AGCGAGAATGTAAGACCTTCCACAATGGACAGGTCAGGTAGCCAGAACACCCATCAGGCGCGTCCTCATCGGCTACGATGTAAAAATGGGTCTGGAAATGAACTTTTCAAGCCTTATCAGCGGACTTTTTTTTGCAGTTTATGGTCTATTGCATAGACTGAGGGGGCAGCACGAAACGTTAAAGTGCGATAGCCGCCACAGTGCGACGTAATGATGATGTTATTTTCAGCAAGGGATCGCTATAACTCATCAGGCCAACACCCACGATCAACAGATGAATTTATTATGATAA  >  NC_000913_3_hsa_tpiA/3867443‑3867734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.