New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	= 2025238	51 (1.200)	4 (0.100)	4/266	5.7	7.7%	noncoding (76/87 nt)	dsrA	sRNA antisense regulator enhances translation of RpoS, Hfq‑dependent
	?	NC_000913_3_hsa_tpiA	= 2025243	49 (1.240)					noncoding (71/87 nt)	dsrA	sRNA antisense regulator enhances translation of RpoS, Hfq‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAA  >  NC_000913_3_hsa_tpiA/2025094‑2025386                                                                                                                                                 |                                                                                                                                                      aGCCAGAACGAGTTTGAACACGTTAGGGTCGATGTACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAg                                                                                                                                                   <  2:545024/149‑1 (MQ=255)            ttCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGTATGCACACTCAAAAAATCCCGCACCTGAGGGGGACGGGAt                                                                                                                                        >  1:100364/1‑149 (MQ=255)              cTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGa                                                                                                                                      >  1:407903/1‑149 (MQ=255)                gAACACGTTAGCGTCGATGGCCGTGAATATCATACATGGCGCGAATTGCCGGATGCATACAAACTGGATATTGATGACTTCAGCGCCACCGAAGCGAAACCATGAATGCACCAAAAAAAAACACCGACCCTGAGGAGGACgggcacaaa                                                                                                                                    >  2:421284/1‑143 (MQ=255)                gAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGaaa                                                                                                                                    <  1:741908/149‑1 (MQ=255)                    acGGTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTg                                                                                                                                <  1:23036/149‑1 (MQ=255)                     cGTTAGCGTCGATGGACGTGAATAGCATACATGGCGGGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTGCAGCGTCTCTGAAGTGAATCGGTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGc                                                                                                                               <  1:113280/149‑1 (MQ=255)                                    aCGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCAc                                                                                                                <  1:222549/149‑1 (MQ=255)                                           tatCATACATGGCGTGACGTGGCGGATGCCTTCGAACTGGATATTCATGGCTTCAGCGTCTCTTAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACACTGAGGTGGTGGGAGTGAAACTTGCTTACGCAAGCAGAACTTcaaga                                                                                                         >  2:343895/1‑147 (MQ=255)                                            atCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTaaaaaa                                                                                                        >  2:541543/1‑149 (MQ=255)                                              cATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCGTGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAAtt                                                                                                      >  1:618787/1‑149 (MQ=255)                                               aTACACGGCGAGATTTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGGCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTc                                                                                                     >  2:659314/1‑149 (MQ=255)                                                   atcgCGAGAACTGGCGGAAGCCCACGAACTGGACATCCATTACTTCAGCATCTCTCAAGTGAATCGTTGAACGCACAATAAAAAAATCCCCACCCTGAGGGGGTCGGGATCAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTa                                                                                                 <  2:46515/146‑1 (MQ=255)                                                   aTGGGGTGAATTGGAGGATGCCTTCGAACTGGATAGTCATGATTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTACAAAATTCGttt                                                                                                 <  2:525102/149‑2 (MQ=255)                                                    tGGCGTGAATGGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGGGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTac                                                                                                <  1:299334/149‑1 (MQ=255)                                                        gTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCCCTTCAAAAATTCGTTACAccg                                                                                            >  2:184085/1‑148 (MQ=255)                                                        gTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCa                                                                                            <  2:407903/149‑1 (MQ=255)                                                         tGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAg                                                                                           >  1:75760/1‑149 (MQ=255)                                                                  ggATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGCTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGa                                                                                  <  2:650054/149‑1 (MQ=255)                                                                   gATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGAt                                                                                 <  1:617035/149‑1 (MQ=255)                                                                     tGCCTTCGAAATGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGAtgt                                                                               <  1:308304/149‑1 (MQ=255)                                                                         ttCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGATTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGAtgcattc                                                                           >  2:548138/1‑144 (MQ=255)                                                                         ttCGAACTGGATATTCATGACTTCAGCGTCTCTAAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGAGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCTTTACAACAGGAAATCGAATATGTtc                                                                           >  2:20676/1‑149 (MQ=255)                                                                         ttCGAACTGAATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCCACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGACGCACTTAAAAAATTCGTTACACCCGGAAATCTGATGCGTtc                                                                           >  2:101482/1‑149 (MQ=255)                                                                               cTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCAcc                                                                     >  1:564231/1‑149 (MQ=255)                                                                                  gATATTCATGACTTCAGCGACTCTGAAGTGAATCGTTGACTGCACAATAAAAAAATCCCGACCCTGAGGGAGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTAAACCAGGAAATCTTATGTGTTCACCACCtcg                                                                  >  2:724059/1‑147 (MQ=255)                                                                                     atTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTCATGAGTTCTTAACCTTATcc                                                               >  1:665404/1‑149 (MQ=255)                                                                                     atTCATGAATTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATcc                                                               <  1:285854/149‑1 (MQ=255)                                                                                              ttCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAAttttt                                                      >  2:641306/1‑149 (MQ=255)                                                                                              ttCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAAttttt                                                      >  1:295579/1‑149 (MQ=255)                                                                                                   cGTCTCTGACGTGAGTGTGTAAATGAAGAATAAAAAAGTCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGc                                                 <  2:340818/149‑1 (MQ=255)                                                                                                       tctGAAGTGAATCGTTGAATGCCCAAAAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTAAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATAACCTCATCAGCAATTTTTTTCGCGGAc                                             >  2:151826/1‑149 (MQ=255)                                                                                                         tGAAGTGGATCGTTGAATGCAAAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACa‑a                                          <  1:637827/149‑1 (MQ=255)                                                                                                           aaGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGa                                        >  1:52569/1‑149 (MQ=255)                                                                                                                aaTCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCaa                                                           <  1:444154/126‑1 (MQ=255)                                                                                                                aaTCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCaa                                                           >  2:444154/1‑126 (MQ=255)                                                                                                                aaTCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAata                                   <  1:19428/149‑1 (MQ=255)                                                                                                                   cGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTc                                <  1:702097/149‑1 (MQ=255)                                                                                                                     ttGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACAACAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGc                              <  1:594956/149‑1 (MQ=255)                                                                                                                       gAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCa                            >  2:565518/1‑149 (MQ=255)                                                                                                                          tGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCACTGATACAGAAGAT‑TTCGCCatat                         >  2:117544/1‑149 (MQ=255)                                                                                                                             acaATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATatgaa                      <  2:295579/149‑1 (MQ=255)                                                                                                                                 tAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGATA‑AGAAAATATTCGCCATatgaatgct                  >  2:614692/1‑147 (MQ=255)                                                                                                                                    aaaaaTCCCGACCCTGAGGGGGTCGGGACGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTaa               <  1:172345/149‑1 (MQ=255)                                                                                                                                      aaaTCACGACCCTGAGGGGGTCAGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACg             <  2:225751/149‑1 (MQ=255)                                                                                                                                            cGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAACTTCGTTCAAACTAGAAATGTGCCGTGTGCATCACCTTATCCGCAATGTTTATCGCTGACA‑AGAAAGTATTCGCCATATGAATGATTAACGCTACtt       >  1:177894/1‑149 (MQ=255)                                                                                                                                            cGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACGCTACtt       >  1:513991/1‑149 (MQ=255)                                                                                                                                              aCCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACGCTACTTAt     <  2:703364/149‑1 (MQ=255)                                                                                                                                                ccTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACGCTACTTATGa   <  1:550799/149‑1 (MQ=255)                                                                                                                                                 cTGAGGGGGTCGGGCTGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACGCAACTTATGaa  >  2:177791/1‑149 (MQ=255)                                                                                                                                                 cTGAGGGGGTCGGGATGAAACTTGCCTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACGCTACTTATGaa  <  1:390134/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                      AGCCAGAACGATTCTGAACACGTTAGCGTCGATGGACGTGAATATCATACATGGCGTGAATTGGCGGATGCCTTCGAACTGGATATTCATGACTTCAGCGTCTCTGAAGTGAATCGTTGAATGCACAATAAAAAAATCCCGACCCTGAGGGGGTCGGGATGAAACTTGCTTAAGCAAGAAGCACTTAAAAAATTCGTTACACCAGGAAATCTGATGTGTTCATCACCTTATCCGCAATTTTTTTCGCTGACA‑AGAAAATATTCGCCATATGAATGATTAACGCTACTTATGAA  >  NC_000913_3_hsa_tpiA/2025094‑2025386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.