Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	1,774,576	G→A	G263E (GGG→GAG)	ydiB →	quinate/shikimate 5‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	1,774,576	0	G	A	100.0%	301.9 / NA	90	G263E (GGG→GAG)	ydiB	quinate/shikimate 5‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/54);  total (36/54)

ATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGATCTCGTCATTG  >  NC_000913_3_hsa_tpiA/1774430‑1774719                                                                                                                                                   |                                                                                                                                                atCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCGAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGc                                                                                                                                              <  2:10761/150‑1 (MQ=255) atCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGc                                                                                                                                              <  2:667289/150‑1 (MQ=255) atCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGc                                                                                                                                              <  1:1039174/150‑1 (MQ=255) atCATTGGTTAACGATATCAGTCTGTTACATCCGGGACTTCTGGTGACTGAATGCGTGTATAACCCGCATATGACGAAGTGATTGCAGCAGGCGCAACATGCTGGGTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGc                                                                                                                                              <  1:669367/150‑1 (MQ=255)     ttGGTTAATGGTATCAGTCTGTAACCTCCGGGACTTCTGGTCACTTAATGCGTGTATAACCCGCATATGACTAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGaa                                                                                                                                          <  2:1026640/150‑1 (MQ=255)     ttGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgagg                                                                                                                                               <  1:252436/145‑1 (MQ=255)     ttGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAgagg                                                                                                                                               >  2:252436/1‑145 (MQ=255)       ggTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACa                                                                                                                                        <  2:444922/150‑1 (MQ=255)        gTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCACCAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAg                                                                                                                                       >  1:163373/1‑150 (MQ=255)         ttAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGt                                                                                                                                      <  1:994305/150‑1 (MQ=255)          tAATGATATCAGTCTGTTACATCCGGTACTTCTGGTCACTGAATGCGTTTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGGAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGtt                                                                                                                                     <  2:62375/150‑1 (MQ=255)           aaTGATCTCATTCTGTTACATCCGGGACTTCTGGTCACTGAATGCCTGGATAAACCGCATATGACGAAATTATTGCAGCAGGCGCAACAAGCTGGTTACAAAACGATTGATGGACACGGCATGTTGTTGCGGCAAGAGGCTGAACAGTTc                                                                                                                                    <  1:441047/150‑1 (MQ=255)            aTGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGAGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTTTGGCCAGACGCTGAACAGTTca                                                                                                                                   >  1:948739/1‑150 (MQ=255)             tGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTcac                                                                                                                                  >  1:1017644/1‑150 (MQ=255)                 atCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTa                                                                                                                              >  2:374793/1‑150 (MQ=255)                  tCAGTCTGTTACATCCGGGACTTCTGGTCAATGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTAt                                                                                                                             <  2:473141/150‑1 (MQ=255)                       cTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACt                                                                                                                        <  1:640637/150‑1 (MQ=255)                             cATCCGGGACTTCTGGTCACTGAATGCGTGTATACCCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCaaa                                                                                                                  <  1:763216/150‑1 (MQ=255)                             cATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCaaa                                                                                                                  <  2:122970/150‑1 (MQ=255)                               tCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGa                                                                                                                <  2:681935/150‑1 (MQ=255)                               tCCGGGACTTATGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGa                                                                                                                <  2:1147450/150‑1 (MQ=255)                                 cGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGAtt                                                                                                              <  1:1010500/150‑1 (MQ=255)                                  gggACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATg                                                                                                                            <  2:456359/135‑1 (MQ=255)                                  gggACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATg                                                                                                                            >  1:456359/1‑135 (MQ=255)                                    gACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTcc                                                                                                           <  1:607862/150‑1 (MQ=255)                                    gACTTCTGGTCACTGAAGCCTTGTATAACCCGCATATTACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTcc                                                                                                           <  1:8672/150‑1 (MQ=255)                                       ttCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCctc                                                                                                        >  1:1081736/1‑150 (MQ=255)                                           ggTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTGACATTATGGACTGGCAAAGATTTCCCTCTGGa                                                                                                    >  1:79230/1‑150 (MQ=255)                                           ggTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTCGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGa                                                                                                    >  1:627650/1‑150 (MQ=255)                                            gTCACTGAATGCGTGTATAACCCGCATATGACAAAGTTATTGCATCAGTCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGaa                                                                                                   <  1:503560/150‑1 (MQ=255)                                               aCTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAatat                                                                                                <  1:433479/150‑1 (MQ=255)                                                 tGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGt                                                                                              >  1:524132/1‑150 (MQ=255)                                                 tGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGt                                                                                              <  1:927633/150‑1 (MQ=255)                                                  taaTGCGTGTATAACCGGCATATGACGAACTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGtt                                                                                             <  2:989642/149‑1 (MQ=255)                                                   aaTGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACatgatg                                                                                                                            >  1:1053738/1‑118 (MQ=255)                                                   aaTGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATg                                                                                                                            <  2:1053738/118‑1 (MQ=255)                                                    aTGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGa                                                                                                                >  2:1174598/1‑129 (MQ=255)                                                    aTGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGa                                                                                                                <  1:1174598/129‑1 (MQ=255)                                                     tgatggtACAACCCGCATATAGCGAAGCTATTGCACCCGGGGTCACTAGCTTGTCGCAAAACGATTGATGGATACGGCATGTTGTTGTTGCAAGAGACTGAACAGTATCCATTATGTACTGTCAAAGCTGTCCATCTGGAATATGTTaat                                                                                          <  2:151868/145‑2 (MQ=255)                                                     tGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCACTCTGGAATCTGTTgaa                                                                                          >  1:492881/1‑150 (MQ=255)                                                       cGTGTATAACCCGCATATGACGAAGTTATCGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGTACTGGCAAAGATTTCCCTCTGGAATATGTTAAACa                                                                                        <  2:10984/150‑1 (MQ=255)                                                            ataACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCa                                                                                   <  1:1108383/150‑1 (MQ=255)                                                             taACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAACACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCAt                                                                                  >  1:821076/1‑150 (MQ=255)                                                                cccGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATggg                                                                               >  1:538155/1‑150 (MQ=255)                                                                 ccGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGaa                                                                                                                                          <  1:11832/90‑1 (MQ=255)                                                                 ccGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGaa                                                                                                                                          >  2:12939/1‑90 (MQ=255)                                                                 ccGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGaa                                                                                                                                          >  2:11832/1‑90 (MQ=255)                                                                 ccGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGaa                                                                                                                                          <  1:12939/90‑1 (MQ=255)                                                                 ccGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATgggg                                                                              >  1:385303/1‑150 (MQ=255)                                                                 ccGCATATGACGAAGTTATTGCAGCAGGCGAAACAATCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATgggg                                                                              >  2:1142187/1‑150 (MQ=255)                                                                    gatgaggcGAAGTTAGTGCAGTAGTTCAAACAAGCTGGTTGCAAAACGATTGATGGATCCGGCATGTTGTTGTGGCAAGAGGCTTAACAGTTCACATTATGGACTGGCAATGATTTCCCTCTGGAATATGTTAAACAGTTCATGGGGTTc                                                                           <  2:233115/143‑1 (MQ=255)                                                                       atGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGt                                                                        <  2:538155/150‑1 (MQ=255)                                                                         gACGAAGTTATTGCAGCAGGGGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGc                                                                      <  2:750064/150‑1 (MQ=255)                                                                            gAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTg                                                                   >  1:344224/1‑150 (MQ=255)                                                                            gAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTg                                                                   >  2:19509/1‑150 (MQ=255)                                                                            gAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGCTCATGGGGTTCGGTGCCTg                                                                   >  2:445614/1‑150 (MQ=255)                                                                             aaGTTATTGCACCAGGCGCAACAAGCTGGTTTCAAAACTATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTCCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGa                                                                  <  1:66031/150‑1 (MQ=255)                                                                               gTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACa                                                                <  2:1081736/150‑1 (MQ=255)                                                                                 tctgtaagcggGCGCAATTAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAgg                                                              <  2:821076/144‑1 (MQ=255)                                                                                 tATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAgg                                                              <  1:374793/150‑1 (MQ=255)                                                                                         cagGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACcgc                                                      <  2:140414/150‑1 (MQ=255)                                                                                                   agggtGGGTGCAAAGCGATTGATGGGTACGGCATGTTGTTGTGGCAAGAGGGTGAACAGTTCACATTATGGACTGGCAAAGAGTGCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAg                                            <  1:1009614/146‑1 (MQ=255)                                                                                                   aaGCTAGTTGCAAAACGATTGATGGATACTGCATGTTGTGGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAg                                            <  1:14024/150‑1 (MQ=255)                                                                                                          ttGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTaaaa                                     >  1:613967/1‑150 (MQ=255)                                                                                                            gCAAAACGAATGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTGAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTaaaaaa                                   <  2:524132/150‑1 (MQ=255)                                                                                                             cAAAACGATTGATGGATGCGGCATGTGGGTGTGGAAAGAGGCGGAACAGTTGATATTATGGACGGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAAt                                  <  2:124783/150‑1 (MQ=255)                                                                                                             cAAAACGATTGATGCATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAAt                                  >  1:1024593/1‑150 (MQ=255)                                                                                                              aaaaCGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATg                                 >  2:138717/1‑150 (MQ=255)                                                                                                               aaaCGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGa                                <  2:1103573/150‑1 (MQ=255)                                                                                                               aaaCGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGa                                <  2:1017644/150‑1 (MQ=255)                                                                                                                aaCGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCACAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGCCCGGCTGACCGCGTGCAGAAAGGGTAAAAAATGaa                               >  2:638260/1‑150 (MQ=255)                                                                                                                aaCGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGATGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGaa                               >  2:1037092/1‑150 (MQ=255)                                                                                                                 aCGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGAtt                                                                                                              >  2:1020615/1‑70 (MQ=255)                                                                                                                 aCGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTTCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGaaa                              >  2:190940/1‑150 (MQ=255)                                                                                                                 aCGATTGATGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATAGACTGGCAAAGAtt                                                                                                              <  1:1020615/70‑1 (MQ=255)                                                                                                                        aTGGATGCGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTaa                       <  2:684849/150‑1 (MQ=255)                                                                                                                         tGGATACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGAGTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTGCGGTGCCTGACAGGCTGACCGCGTGCACAAAGGGGAAAAGATTAAAACCGTAAc                      >  2:444559/1‑150 (MQ=255)                                                                                                                            aTACGGCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCGTCTGGAATATGTTAAACAGGTCATGTGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGt                   <  2:118853/150‑1 (MQ=255)                                                                                                                                ggCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAgg                                                              >  2:205058/1‑103 (MQ=255)                                                                                                                                ggCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAgg                                                              <  1:205058/103‑1 (MQ=255)                                                                                                                                ggCATGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTaaaa               >  2:82033/1‑150 (MQ=255)                                                                                                                                   aTGTTGTTGTGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGAt            <  1:509775/150‑1 (MQ=255)                                                                                                                                      ttgttgtGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGGAAAAAATGAAAACCGTAACTGTAAACGAtctc         >  2:365515/1‑150 (MQ=255)                                                                                                                                        gttgtGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCACTGAGATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGATCTCGt       >  1:821240/1‑150 (MQ=255)                                                                                                                                         ttgtGGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGATCTCGTc      >  1:395106/1‑150 (MQ=255)                                                                                                                                          tgtgGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGATCTCGTCa     <  1:138717/150‑1 (MQ=255)                                                                                                                                           gtgGCAAGAGGCTGAACAGTTCACATTATGGACTGGTAAGAATTTCCTGCTGTAAGATGTTAAACAGGTCATGGGGTTCGGTGCCTGCCAGGCTGACCGCGTGCAGAAAGGGT‑AAAAATGAAAACCGTAACTGTAAAAGATCTCGTCAtt   <  2:1010836/150‑1 (MQ=255)                                                                                                                                           gtgGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGATCTCGTCAt    <  2:344224/150‑1 (MQ=255)                                                                                                                                            tgGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTaaaaaa                                   <  2:613964/118‑1 (MQ=255)                                                                                                                                            tgGCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTaaaaaa                                   >  1:613964/1‑118 (MQ=255)                                                                                                                                            tgGCAAGAGGCTGAACAGTTCACATTATGGACTGGAAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGGGCCTGACAGGCTGACCGCGTGCAGAAAGGGAAAAAAATGAAAACCGTAACTGTAAAAACTCTCGTCAtt   >  2:604675/1‑150 (MQ=255)                                                                                                                                             ggCAAGAGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGATCTCGTCATTg  <  2:862057/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                ATCATTGGTTAATGATATCAGTCTGTTACATCCGGGACTTCTGGTCACTGAATGCGTGTATAACCCGCATATGACGAAGTTATTGCAGCAGGCGCAACAAGCTGGTTGCAAAACGATTGATGGATACGGCATGTTGTTGTGGCAAGGGGCTGAACAGTTCACATTATGGACTGGCAAAGATTTCCCTCTGGAATATGTTAAACAGGTCATGGGGTTCGGTGCCTGACAGGCTGACCGCGTGCAGAAAGGGTAAAAAATGAAAACCGTAACTGTAAAAGATCTCGTCATTG  >  NC_000913_3_hsa_tpiA/1774430‑1774719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑