New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_hsa_tpiA	1805292 =	33 (0.670)	5 (0.100)	5/276	6.3	12.6%	intergenic (+20/+33)	yniD/ydiY	uncharacterized protein/acid‑inducible putative outer membrane protein
	?	NC_000913_3_hsa_tpiA	1805317 =	37 (0.760)					intergenic (+45/+8)	yniD/ydiY	uncharacterized protein/acid‑inducible putative outer membrane protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GAGATATGCAGGACACTGGTATGCCGACTAAACGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTC  >  NC_000913_3_hsa_tpiA/1805145‑1805438                                                                                                                                                    |                                                                                                                                                   gagaTATGCAGGACACTGGTATGCCGACTAAACGCTTTGATAAAAAACACTGGGAGAGGCTTGTGCTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGCCAAACGAAGACAGc                                                                                                                                                   >  1:604815/1‑149 (MQ=255) gagaTATGCAGGACACTGGTATGCCGACTAAACGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGc                                                                                                                                                   <  2:806728/149‑1 (MQ=255)          aGGACACTGGTATGCCGACTAAACGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCa                                                                                                                                          <  2:267572/149‑1 (MQ=255)                         cGACTAAACGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCa                                                                                                                           <  1:56888/149‑1 (MQ=255)                               aaCGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCatatctc                                                                                                                     <  2:604815/149‑7 (MQ=255)                                    tttGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTAc                                                                                                                >  1:492565/1‑149 (MQ=255)                                       gATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATg                                                                                                             >  1:267647/1‑149 (MQ=255)                                         tAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCt                                                                                                           >  1:52782/1‑149 (MQ=255)                                                     gAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGa                                                                                               >  1:270644/1‑149 (MQ=255)                                                       aGATGGTGGTGGTGCTACTCGGAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACCCGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGGTa                                                                                             >  2:322646/1‑149 (MQ=255)                                                            gtggtggtgCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGtt                                                                                        >  2:652950/1‑149 (MQ=255)                                                                             aaTCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATg                                                                       >  1:464463/1‑149 (MQ=255)                                                                               tcaggggCGCCATTATGTTGCTACTTCGGGCAGCAATGATTTGGAGCTGAGCAGGGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCTGTATGtt                                                                     <  2:107536/144‑1 (MQ=255)                                                                                        cTAGGTTGTTGCTACGTTGGGCAGCACTGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAg                                                            >  2:416845/1‑149 (MQ=255)                                                                                               gttgCTACGTTGGGCAGGAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCAGGCAGATAATTTTACATGCTGTAGCCAAGCGATAAGGTTGTTCTACGGTCGGTATGTTCCGGTGCAGACTCTgg                                                     <  2:289252/149‑1 (MQ=255)                                                                                                ttgCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGa                                                    >  1:786539/1‑149 (MQ=255)                                                                                                ttgCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGa                                                    <  2:651384/149‑1 (MQ=255)                                                                                                 tgatagcttGGGCAGCAAGGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAg                                                   <  2:394640/142‑1 (MQ=255)                                                                                                   cTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGa                                                    >  1:336773/1‑146 (MQ=255)                                                                                                   cTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGa                                                    <  2:336773/146‑1 (MQ=255)                                                                                                                caATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCAtg                                    >  2:467509/1‑149 (MQ=255)                                                                                                                   tGATTGGGGGCGGAGGAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGa                                 <  2:106982/149‑1 (MQ=255)                                                                                                                       ttGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGtt                             <  2:447380/149‑1 (MQ=255)                                                                                                                       ttGGGGCTGAGCAGGGACAAACGAAGCCAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGtt                             <  1:257776/149‑1 (MQ=255)                                                                                                                           ggcggggCAGTGACAAACGAGGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTaa                         <  2:666140/143‑1 (MQ=255)                                                                                                                             cTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGAt                                                                                              >  2:27933/1‑78 (MQ=255)                                                                                                                             cTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGAt                                                                                              <  1:27933/78‑1 (MQ=255)                                                                                                                                   aGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCtt                 <  2:790038/149‑1 (MQ=255)                                                                                                                                   aGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCAGTATGTTCCGGTGCAGAGCCTGCAATTTGATATGTCCATGTGACGTTGTAAGCCACCtt                 <  2:840532/149‑1 (MQ=255)                                                                                                                                         aaaCGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTcc           >  2:297860/1‑149 (MQ=255)                                                                                                                                         aaaCGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGGATGTTCCGGTGCAGACTCTGGCGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTcc           >  1:269079/1‑149 (MQ=255)                                                                                                                                                 aCAGCCAGACCGCATAACGCGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGtt   <  1:138944/149‑1 (MQ=255)                                                                                                                                                  cAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTc  <  1:509450/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GAGATATGCAGGACACTGGTATGCCGACTAAACGCTTTGATAAAAAACACTGGAAGATGGTGGTGGTGCTACTGGCAATCTGTGGCGCTATGTTGTTGCTACGTTGGGCAGCAATGATTTGGGGCTGAGCAGTGACAAACGAAGACAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGTTCCGGTGCAGACTCTGGAGGTTCAGAGTTCCATGTGACGTTGTAAGCCACCTTCAGTCCAAAATGTTC  >  NC_000913_3_hsa_tpiA/1805145‑1805438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.