New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_hsa_tpiA 2375824 =6 (0.120)42 (0.870) 33/274 0.6 87.8% coding (139/963 nt) menC O‑succinylbenzoyl‑CoA synthase
?NC_000913_3_hsa_tpiA = 2515613 NA (NA)intergenic (+170/+30) insL1/yfeA IS186 transposase/putative diguanylate cyclase

TCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAAT  >  NC_000913_3_hsa_tpiA/2515473‑2515760
                                                                                                                                            |                                                                                                                                                   
tCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAAt                                                                                                                                             >  1:446610/1‑149 (MQ=32)
   ggtgatgaCAGGTGGCCATTCGGGGGACTGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTGAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTcc                                                                                                                                          <  1:422851/142‑1 (MQ=11)
         tACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTCGCGCTTATGGGATAATTgccttgtt                                                                                                                                    <  2:586114/149‑6 (MQ=255)
              gTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGttttttt                                                                                                                               >  2:542961/1‑149 (MQ=40)
                    aTTCGTGGGACAGTACCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCt                                                                                                                         <  1:287903/149‑1 (MQ=255)
                          gggACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACCCCTGTTCAt                                                                                                                   >  2:199360/1‑149 (MQ=255)
                            gACAGTATCCATGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCa                                                                                                                 <  2:723349/149‑1 (MQ=255)
                                gTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATAAGCTa                                                                                                             >  1:274694/1‑149 (MQ=255)
                                 tATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGCCGTCGCATTCTTCAGAGGGTTCAACTCTTAAGTTAGCGCTTATGGGATTATTCCCCCGCTTTTTTACGCCTGTTAACTAGCTac                                                                                                            >  2:831877/1‑148 (MQ=14)
                                  aTCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAAt                                                                                                           <  1:362724/149‑1 (MQ=255)
                                    cccTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATgg                                                                                                         >  2:334540/1‑149 (MQ=255)
                                      cTGACAGCCTACAAAACGCAATTGAAGACAGCTAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCt                                                                                                       >  1:210186/1‑149 (MQ=255)
                                          cAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGGGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCgg                                                                                                   <  1:57001/149‑1 (MQ=255)
                                              cTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGa                                                                                               <  1:369096/149‑1 (MQ=255)
                                               tACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGGCGCATTCTTCAGATGGTTCAACCCTTAAGTTCGCGCTTGTGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCCGCTAATGGCGGCGGGcagc                                                                                              >  1:70684/1‑146 (MQ=18)
                                                aCAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCATCTAATGGCTGCGGGCGAcc                                                                                             >  2:506354/1‑149 (MQ=255)
                                                       gCAATTGAAGAACGCGAGGGATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCaaa                                                                                      <  1:204078/149‑1 (MQ=255)
                                                         aaTTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTc                                                                                                                                           <  1:294635/94‑1 (MQ=35)
                                                         aaTTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTc                                                                                                                                           >  2:294635/1‑94 (MQ=35)
                                                          aTTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATAc                                                                                   >  2:44827/1‑149 (MQ=255)
                                                           ttGGAGAACGCGAGGCATCGTTTTAACGAGGGACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATAcc                                                                                  <  2:95885/149‑1 (MQ=255)
                                                                gaaCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGGTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGc                                                                             >  1:439516/1‑149 (MQ=255)
                                                                 aaCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATgg                                                                                                         <  2:817874/120‑1 (MQ=255)
                                                                 aaCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATgg                                                                                                         >  1:817874/1‑120 (MQ=255)
                                                                 aaCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCtt                                                                               <  1:359512/146‑1 (MQ=255)
                                                                 aaCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCtt                                                                               >  2:359512/1‑146 (MQ=255)
                                                                 aaCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCa                                                                            <  1:474810/149‑1 (MQ=255)
                                                                  ccgcgAGGGATCGTCTTAACGGGGCACCGAGGCGTCGCATTCTTGAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAg                                                                           <  2:130607/148‑1 (MQ=255)
                                                                  aCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGttttt                                                                                                                                 <  2:363077/95‑1 (MQ=255)
                                                                  aCGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGttttt                                                                                                                                 >  1:363077/1‑95 (MQ=255)
                                                                         gCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCACCTAATGGCTGCGGGCGACCAATCACATACCCTTGCAGATCTTGc                                                                    >  2:246680/1‑149 (MQ=255)
                                                                          cATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCa                                                                   <  1:334540/149‑1 (MQ=255)
                                                                                 ttAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCACTCAAATACCCTTGAATATATTGCACCCCGAg                                                            >  2:146036/1‑149 (MQ=255)
                                                                                       aGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATg                                                      <  1:675580/149‑1 (MQ=255)
                                                                                         gCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCa                                                    >  2:39300/1‑149 (MQ=255)
                                                                                           aCCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAACTACCCTTGCACATATTGCACCCCCACCTTATGCCAt                                                  >  2:336548/1‑149 (MQ=37)
                                                                                               aGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGGTAGCGCTTATGGGATAATTCCCCGGTTGTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACAAATCAAATACCTTTCGAGATATTGCACGCCGGGCTTATGAAATAgcg                                              >  1:187651/1‑149 (MQ=37)
                                                                                                ggCGTCCCATTCTCCAGATGGTTCAACCCTCAAGTTAGCGCTTATGGGATAATTCCCCAGGTTTTTTACGCCTGTTAATCAGCTAACGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAgcgc                                             <  1:201445/149‑1 (MQ=255)
                                                                                                         ttcttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCctgctgctg                                    <  1:542961/149‑1 (MQ=255)
                                                                                                            ttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTc                                                                                                                                           <  1:633421/43‑1 (MQ=255)
                                                                                                            ttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTc                                                                                                                                           >  2:633421/1‑43 (MQ=255)
                                                                                                            ttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTTTTTTTTTTTATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGACCTTATGCAATACCGCCTGCTGCTTTTg                                 >  2:128948/1‑149 (MQ=38)
                                                                                                            ttcAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTg                                 >  2:47149/1‑149 (MQ=255)
                                                                                                                  tGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCCATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGtctc                           <  1:319695/149‑1 (MQ=255)
                                                                                                                     ttCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGAc                        >  1:46459/1‑149 (MQ=255)
                                                                                                                     ttCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAAAGGGGCCTGCTGCTGTTGCGTCTCGAc                        >  1:136324/1‑149 (MQ=255)
                                                                                                                        aaCCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCGGTTCCGTCTCGACaaa                     >  2:740883/1‑149 (MQ=255)
                                                                                                                           ccTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGACCTTATGCAATACGGCCTGCTGCTGTTCCGTCCCGACAAACTc                  >  2:811647/1‑149 (MQ=255)
                                                                                                                            cTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTcc                 >  1:520819/1‑149 (MQ=255)
                                                                                                                             ttAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGCCAAACTccc                >  2:239487/1‑148 (MQ=255)
                                                                                                                                         ttAGGGGATAATTCCCCGGATTTTTTAAGGATGTTAATCAGCTAAGGGCTGCGGGCGACCAATCGAATACCCTTGCCGATATTGCACCCGGAGCTTATGCAATAAAGCCTGCTGCTGTTGCGTCTCGACAAAATCCGCGACACACACaa   >  2:536491/1‑149 (MQ=255)
                                                                                                                                          tATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCaa   <  1:608622/149‑1 (MQ=255)
                                                                                                                                           aTGGGATAATTCCCCGGTTTTTTTACGCCTGTCAAACACTTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAAt  <  1:676466/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
TCTGGAGCTTACAGGTGGCCATTCGTGGGACAGTATCCCTGACAGCCTACAAAACGCAATTGAAGAACGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGATAATTCCCCGGTTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATCAAATACCCTTGCAGATATTGCACCCCGAGCTTATGCAATAGCGCCTGCTGCTGTTGCGTCTCGACAAACTCCGCGACCACACTCAAT  >  NC_000913_3_hsa_tpiA/2515473‑2515760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.