Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	4,183,363	G→A	G713S (GGT→AGT)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	4,183,363	0	G	A	100.0%	228.3 / NA	66	G713S (GGT→AGT)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/39);  total (27/39)

CCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCA  >  NC_000913_3_hsa_tpiA/4183224‑4183508                                                                                                                                            |                                                                                                                                                  ccTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTg                                                                                                                                          >  1:238270/1‑149 (MQ=255)    gATCCCGTTCCTGGAACACGATGCCGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCgg                                                                                                                                       <  1:733395/149‑1 (MQ=255)      tCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTa                                                                                                                                     <  1:695674/149‑1 (MQ=255)              cTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACg                                                                                                                             >  2:232326/1‑149 (MQ=255)               tGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGCGTAACTGCGGTCGGTACACgt                                                                                                                            >  2:370929/1‑149 (MQ=255)                  aaCACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACgtggt                                                                                                                         <  1:232326/149‑1 (MQ=255)                       gATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGgtgt                                                                                                                    >  2:12650/1‑149 (MQ=255)                        aTGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTc                                                                                                                   <  1:827104/149‑1 (MQ=255)                           aCGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGtt                                                                                                                <  1:490024/149‑1 (MQ=255)                             gCCAACCGTGCATTGATGGGTTCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCa                                                                                                              <  2:870115/149‑1 (MQ=255)                               cAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGt                                                                                                            <  1:12650/149‑1 (MQ=255)                               cAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGt                                                                                                            <  2:123001/149‑1 (MQ=255)                                    gTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTg                                                                                                       <  1:592412/149‑1 (MQ=255)                                            aTGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTc                                                                                               <  1:448529/149‑1 (MQ=255)                                             tGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTcc                                                                                              >  1:595704/1‑149 (MQ=255)                                              gggTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGt                                                                                                            <  1:119838/134‑1 (MQ=255)                                              gggTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGt                                                                                                            >  2:119838/1‑134 (MQ=255)                                                gTGCGAACAGGCAACGGCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGt                                                                                           <  2:634176/149‑1 (MQ=255)                                                 tGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTa                                                                                          <  2:18353/149‑1 (MQ=255)                                                    gAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCg                                                                                       >  2:566558/1‑149 (MQ=255)                                                     aaCATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGt                                                                                      <  2:684984/149‑1 (MQ=255)                                                      aCATGCAACGTCAGGCCGTTCCGACTCTGCGCGTTGATAAGCCGCTGGTTGGTACTGGTAGGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGGGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGtt                                                                                     <  1:744866/149‑1 (MQ=255)                                                           cAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGCTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGCAGCTGCTTCCCGTATCGTTATCaa                                                                                >  2:790285/1‑149 (MQ=255)                                                            aaagtgaGGCCGTGCCGTCTCCGCGGGTTGATCAGCCGGTGGTTGGTGCTGGTATGGAACGTGCTGTTGCCGTTGACTTCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCaaa                                                                               <  2:482679/143‑1 (MQ=255)                                                            aaCGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCaaa                                                                               <  1:756163/149‑1 (MQ=255)                                                            aaCGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCaaa                                                                               <  2:459744/149‑1 (MQ=255)                                                            aaCGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAAAGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCaaa                                                                               <  2:346837/149‑1 (MQ=255)                                                                tCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGTATGCGTCCCGTATCGTTATCaacgcta                                                                           >  1:270932/1‑144 (MQ=255)                                                                       ggtCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGa                                                                    <  2:753473/147‑1 (MQ=255)                                                                        ttCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTCTTCCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGAc                                                                   <  1:852371/149‑1 (MQ=255)                                                                          ccGACTCTGCGCGGTGGTAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGGAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACga                                                                 <  2:328613/149‑1 (MQ=255)                                                                          ccGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACga                                                                 <  1:61358/149‑1 (MQ=255)                                                                           cGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACgag                                                                <  2:346331/149‑1 (MQ=255)                                                                            gACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGACCGAGCTTTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACgaga                                                               <  1:67737/149‑1 (MQ=255)                                                                                         gATAAGCCGATGGTTGGGACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGAGGTATCCGGGTGa                                                  >  2:323560/1‑149 (MQ=255)                                                                                          aTAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTGGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGaa                                                 <  2:180339/149‑1 (MQ=255)                                                                                             aGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCa                                              <  1:442554/149‑1 (MQ=255)                                                                                              gCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAg                                             <  2:238270/149‑1 (MQ=255)                                                                                                cGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGt                                           <  1:566558/149‑1 (MQ=255)                                                                                                cGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGCGGTGGTGTCGGGTAGTACGTGGATGCTTCCCGTATCGTTTTCAAGGTTCACGAAGACGAGATGTATCCTGGTGAAGCAGGt                                           >  2:576901/1‑149 (MQ=255)                                                                                                   tggttggtACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATc                                        <  2:595704/149‑1 (MQ=255)                                                                                                   tggttggtACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCCAATCGGTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATc                                        <  1:606772/149‑1 (MQ=255)                                                                                                     gttggtACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGa                                      <  1:276380/149‑1 (MQ=255)                                                                                                       tggtACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACa                                    <  2:470262/149‑1 (MQ=255)                                                                                                        ggtACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGATACCCGTTAATGTTTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACAt                                   <  1:639403/149‑1 (MQ=255)                                                                                                          tACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTTGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCt                                 <  2:17001/149‑1 (MQ=255)                                                                                                          tACTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCt                                 >  1:703822/1‑149 (MQ=255)                                                                                                           aCTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTa                                >  2:854183/1‑149 (MQ=255)                                                                                                            cTGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTAc                               >  2:704398/1‑149 (MQ=255)                                                                                                             tGGTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGGCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGAAGGTATCGACATCTACa                              >  2:370974/1‑149 (MQ=255)                                                                                                              ggTATGGAACGTGCTGTTGCCGTTGACTCCAGTGGACCTGCGGTAGCTAAACGAGCTACCCTCGTTCTCTACGTGTTTGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACaa                             <  2:96380/149‑1 (MQ=255)                                                                                                               gTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTCTCCGGGTGAAGCAGATCTCGACATCTACAAc                            >  2:635613/1‑149 (MQ=255)                                                                                                               gTATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCAGGGTGAAGCAGGTATCGACATCTACAAc                            >  2:475399/1‑149 (MQ=255)                                                                                                                tATGGAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACaaac                           <  1:830053/149‑3 (MQ=255)                                                                                                                   ggAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGa                        >  2:535596/1‑149 (MQ=255)                                                                                                                    gAACGTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGAc                       >  1:556062/1‑149 (MQ=255)                                                                                                                        gTGCTGTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCaaa                   <  1:576901/149‑1 (MQ=255)                                                                                                                         tGCTGTTGCCGTGGACTCCAGTGTAACTGCGGTAGCAACACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGCCAGGTCTCCGGGTGAAGCAGGTATCGCCATCTACCACCTGACCAAAt                  >  2:316621/1‑149 (MQ=255)                                                                                                                             gTTGCCGTTGACTCCAGTGTAACTGGGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACAATATacac              >  1:788843/1‑149 (MQ=255)                                                                                                                             gTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATc                                                                                        <  2:720862/75‑1 (MQ=255)                                                                                                                             gTTGCCGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATc                                                                                        >  1:720862/1‑75 (MQ=255)                                                                                                                                  cGTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGtt         >  2:194715/1‑149 (MQ=255)                                                                                                                                   gTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTc        >  1:266397/1‑149 (MQ=255)                                                                                                                                   gTTGACTCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTATCGTTCAGTACGTGGAAGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGCTATCGACCTCAACAACCTGACAACATACATCCGTTc        >  2:120573/1‑149 (MQ=255)                                                                                                                                         tCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAAcaa  >  2:349317/1‑147 (MQ=255)                                                                                                                                         tCCAGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCa  >  1:291597/1‑149 (MQ=255)                                                                                                                                            |                                                                                                                                                  CCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGAACGTGCTGTTGCCGTTGACTCCGGTGTAACTGCGGTAGCTAAACGTGGTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCA  >  NC_000913_3_hsa_tpiA/4183224‑4183508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑