Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	4,111,530	+T	intergenic (+845/+67)	cdh → / ← yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	4,111,530	1	.	T	100.0%	155.7 / NA	45	intergenic (+845/+67)	cdh/yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (25/20);  total (25/20)

ACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCC‑GCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTT  >  NC_000913_3_hsa_tpiA/4111389‑4111669                                                                                                                                               |                                                                                                                                            aCCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCg                                                                                                                                       >  1:465335/1‑149 (MQ=255)   cTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCa                                                                                                                                     >  1:340579/1‑149 (MQ=255)   cTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCa                                                                                                                                     >  2:238076/1‑149 (MQ=255)         cgcgTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGgcag                                                                                                                               >  1:336418/1‑149 (MQ=255)         cgcgTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGgcag                                                                                                                               >  1:75986/1‑149 (MQ=255)                   gTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCtgtg                                                                                                                     >  2:525901/1‑149 (MQ=255)                              cTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTGTag                                                                                                          >  1:7069/1‑149 (MQ=255)                               tCCCCCAGACTGTGCTTCCCCCCGTTCATCTTCCATTTTCCCCCAACGAAGAACTGCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATaga                                                                                                         <  2:408782/149‑1 (MQ=255)                                 ccccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGTTCTGTGAAACAGTATAgaga                                                                                                       >  1:75253/1‑149 (MQ=255)                                 ccccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAgaga                                                                                                       <  1:330906/149‑1 (MQ=255)                                                  gCCCGTTCATCTTCCAGTTTCCCCCAAAGAAGAACGTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGTCCTGCCCCGCAGGGCAGCAGGTCTGTGTAACAGTATAGAGATTCATCGGCACAAAGgc                                                                                      >  2:83562/1‑149 (MQ=255)                                                     cGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgcttt                                                                                   <  2:652782/149‑1 (MQ=255)                                                      gTTCATCTTCCAGTTTCCCCCAACGCAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAATgctttg                                                                                  >  1:651926/1‑149 (MQ=255)                                                          aTCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAAgctttgctgt                                                                              >  2:256357/1‑147 (MQ=255)                                                           tCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGAGTCATCGGCACAAAGGCTTTGCtttt                                                                             >  1:709099/1‑149 (MQ=255)                                                            cTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCttttt                                                                            <  1:281447/149‑1 (MQ=255)                                                            cTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCttttt                                                                            <  2:256284/149‑1 (MQ=255)                                                             ttCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGTGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTg                                                                           >  1:126048/1‑149 (MQ=255)                                                               ccAGTTTCCCCCAACGAAGAACTTCCTGGAGTGCGCCATTTTCATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTc                                                                         <  1:277845/149‑1 (MQ=255)                                                               ccAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTc                                                                         >  1:188373/1‑149 (MQ=255)                                                                 aGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTTTCCACGCTTGTAAGCGTATAAAGGACGATGGCCTGCCCCGCTGGGCAGCAGGTCTGTGGAACAGTATAGAGATTCATCGGTACAAAGGCTTTGCTTTTTCTCAt                                                                       >  1:607978/1‑149 (MQ=255)                                                                      accccACTGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTAtt                                                                  <  2:75986/148‑1 (MQ=255)                                                                        cccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCa                                                                >  1:311859/1‑149 (MQ=255)                                                                         ccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCaa                                                               <  1:597499/149‑1 (MQ=255)                                                                          cAACGAAGAACTTCCTGGAGGGCGCCCATTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCaaa                                                              <  1:17324/149‑1 (MQ=255)                                                                          cAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAGGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCaaa                                                              >  2:122181/1‑149 (MQ=255)                                                                              gcagaaCTTCCTGGCGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGACGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGCCACAAACGCTCTGCTTTTCGTCATTTACTCAAACCtt                                                          >  2:678432/3‑149 (MQ=255)                                                                                 gaaCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCaa                                                       >  2:68603/1‑149 (MQ=255)                                                                                  aaCTTCCTGGAGGGCGCCATTCTAATTCTCCACGCTTATCCGAGACCCTGACAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAg                                                      <  1:525901/149‑1 (MQ=255)                                                                                     ttCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGa                                                   >  2:383601/1‑149 (MQ=255)                                                                                        cTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTc                                                <  2:336418/149‑1 (MQ=255)                                                                                          ggAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAg                                              >  1:89841/1‑149 (MQ=255)                                                                                           gAGGGCGCCATTTTAATTCTCCACGCTTATAAGTGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGa                                             <  2:126048/149‑1 (MQ=255)                                                                                                    aTTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAg                                    <  2:188373/149‑1 (MQ=255)                                                                                                         aaTTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCACCTTaa                               >  2:71124/1‑149 (MQ=255)                                                                                                           ttCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATc                             >  1:3670/1‑149 (MQ=255)                                                                                                            tctcCACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCACGCGATTCAGATAGCGCCAGCTTAATCg                            <  1:54003/149‑1 (MQ=255)                                                                                                               ccACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGtt                         <  2:89841/149‑1 (MQ=255)                                                                                                                cACGCTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGGAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTc                        <  1:238076/149‑1 (MQ=255)                                                                                                                    cTTATAAGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACa                    <  1:250005/149‑1 (MQ=255)                                                                                                                          aGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGAt                                            <  2:125696/119‑1 (MQ=255)                                                                                                                          aGCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGAt                                            >  1:125696/1‑119 (MQ=255)                                                                                                                           gCGAATAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAgg             >  1:89700/1‑149 (MQ=255)                                                                                                                                tAAAGGAAGATGGCCTGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCaac        <  1:336039/149‑3 (MQ=255)                                                                                                                                      aaGATGGCCTGCCCCGCTGGGCTGCAGCTCTGTGAAACAGTATAGAGATTCATCAGTACAAAGGCTTTGCTTCTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAAGAGCGAAGGTCTACCCCttt  <  2:607978/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                            ACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCC‑GCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTT  >  NC_000913_3_hsa_tpiA/4111389‑4111669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑